This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 104.76 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 90,093 (2.8%) Reads that were too short: 16,405 (0.5%) Reads written (passing filters): 73,688 (2.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 20,248,677 bp (2.1%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 90093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1305 777.6 0 1305 7 4043 777.6 0 4043 8 1254 777.6 0 1254 9 56130 777.6 0 56130 10 81 777.6 0 81 11 390 777.6 0 390 12 19 777.6 0 19 13 1 777.6 0 1 14 4 777.6 0 4 15 3 777.6 0 3 16 4 777.6 0 4 17 5 777.6 0 5 18 3 777.6 0 3 19 3 777.6 0 3 20 1 777.6 0 1 21 1 777.6 0 1 22 4 777.6 0 4 23 3 777.6 0 3 24 9 777.6 0 9 25 2 777.6 0 2 26 6 777.6 0 6 27 5 777.6 0 5 28 2 777.6 0 2 29 7 777.6 0 7 30 5 777.6 0 5 31 5 777.6 0 5 32 5 777.6 0 5 33 2 777.6 0 2 34 4 777.6 0 4 35 2 777.6 0 2 36 2 777.6 0 2 37 10 777.6 0 10 38 4 777.6 0 4 39 198 777.6 0 198 40 10 777.6 0 10 41 3 777.6 0 3 42 7 777.6 0 7 43 8 777.6 0 8 44 6 777.6 0 6 45 11 777.6 0 11 46 3 777.6 0 3 47 4 777.6 0 4 48 4 777.6 0 4 49 16 777.6 0 16 50 4 777.6 0 4 51 9 777.6 0 9 52 2 777.6 0 2 53 10 777.6 0 10 54 11 777.6 0 11 55 4 777.6 0 4 56 14 777.6 0 14 57 2 777.6 0 2 58 6 777.6 0 6 59 18 777.6 0 18 60 11 777.6 0 11 61 44 777.6 0 44 62 71 777.6 0 71 63 70 777.6 0 70 64 87 777.6 0 87 65 3 777.6 0 3 66 13 777.6 0 13 67 12 777.6 0 12 68 9 777.6 0 9 69 9 777.6 0 9 70 13 777.6 0 13 71 11 777.6 0 11 72 9 777.6 0 9 73 10 777.6 0 10 74 11 777.6 0 11 75 17 777.6 0 17 76 33 777.6 0 33 77 35 777.6 0 35 78 595 777.6 0 595 79 517 777.6 0 517 80 496 777.6 0 496 81 588 777.6 0 588 82 125 777.6 0 125 83 92 777.6 0 92 84 7 777.6 0 7 85 5 777.6 0 5 86 7 777.6 0 7 87 6 777.6 0 6 88 7 777.6 0 7 89 5 777.6 0 5 90 6 777.6 0 6 91 5 777.6 0 5 92 15 777.6 0 15 93 10 777.6 0 10 94 15 777.6 0 15 95 12 777.6 0 12 96 11 777.6 0 11 97 12 777.6 0 12 98 13 777.6 0 13 99 15 777.6 0 15 100 33 777.6 0 33 101 16 777.6 0 16 102 21 777.6 0 21 103 13 777.6 0 13 104 23 777.6 0 23 105 4 777.6 0 4 106 4 777.6 0 4 107 10 777.6 0 10 108 13 777.6 0 13 109 39 777.6 0 39 110 25 777.6 0 25 111 11 777.6 0 11 112 16 777.6 0 16 113 14 777.6 0 14 114 18 777.6 0 18 115 14 777.6 0 14 116 699 777.6 0 699 117 147 777.6 0 147 118 158 777.6 0 158 119 28 777.6 0 28 120 20 777.6 0 20 121 24 777.6 0 24 122 51 777.6 0 51 123 37 777.6 0 37 124 27 777.6 0 27 125 28 777.6 0 28 126 15 777.6 0 15 127 31 777.6 0 31 128 47 777.6 0 47 129 20 777.6 0 20 130 34 777.6 0 34 131 41 777.6 0 41 132 27 777.6 0 27 133 27 777.6 0 27 134 18 777.6 0 18 135 41 777.6 0 41 136 60 777.6 0 60 137 66 777.6 0 66 138 55 777.6 0 55 139 38 777.6 0 38 140 34 777.6 0 34 141 84 777.6 0 84 142 59 777.6 0 59 143 35 777.6 0 35 144 33 777.6 0 33 145 30 777.6 0 30 146 25 777.6 0 25 147 40 777.6 0 40 148 13 777.6 0 13 149 19 777.6 0 19 150 25 777.6 0 25 151 34 777.6 0 34 152 23 777.6 0 23 153 33 777.6 0 33 154 27 777.6 0 27 155 52 777.6 0 52 156 41 777.6 0 41 157 57 777.6 0 57 158 50 777.6 0 50 159 46 777.6 0 46 160 46 777.6 0 46 161 45 777.6 0 45 162 79 777.6 0 79 163 52 777.6 0 52 164 62 777.6 0 62 165 43 777.6 0 43 166 60 777.6 0 60 167 74 777.6 0 74 168 107 777.6 0 107 169 38 777.6 0 38 170 112 777.6 0 112 171 63 777.6 0 63 172 87 777.6 0 87 173 54 777.6 0 54 174 17 777.6 0 17 175 31 777.6 0 31 176 31 777.6 0 31 177 75 777.6 0 75 178 74 777.6 0 74 179 73 777.6 0 73 180 95 777.6 0 95 181 902 777.6 0 902 182 119 777.6 0 119 183 430 777.6 0 430 184 62 777.6 0 62 185 59 777.6 0 59 186 68 777.6 0 68 187 132 777.6 0 132 188 75 777.6 0 75 189 54 777.6 0 54 190 331 777.6 0 331 191 128 777.6 0 128 192 90 777.6 0 90 193 37 777.6 0 37 194 24 777.6 0 24 195 54 777.6 0 54 196 45 777.6 0 45 197 127 777.6 0 127 198 46 777.6 0 46 199 34 777.6 0 34 200 34 777.6 0 34 201 40 777.6 0 40 202 60 777.6 0 60 203 68 777.6 0 68 204 94 777.6 0 94 205 70 777.6 0 70 206 70 777.6 0 70 207 49 777.6 0 49 208 31 777.6 0 31 209 72 777.6 0 72 210 104 777.6 0 104 211 65 777.6 0 65 212 62 777.6 0 62 213 78 777.6 0 78 214 98 777.6 0 98 215 68 777.6 0 68 216 77 777.6 0 77 217 216 777.6 0 216 218 147 777.6 0 147 219 108 777.6 0 108 220 51 777.6 0 51 221 118 777.6 0 118 222 78 777.6 0 78 223 88 777.6 0 88 224 52 777.6 0 52 225 309 777.6 0 309 226 542 777.6 0 542 227 584 777.6 0 584 228 149 777.6 0 149 229 89 777.6 0 89 230 66 777.6 0 66 231 38 777.6 0 38 232 56 777.6 0 56 233 53 777.6 0 53 234 71 777.6 0 71 235 81 777.6 0 81 236 63 777.6 0 63 237 220 777.6 0 220 238 234 777.6 0 234 239 88 777.6 0 88 240 44 777.6 0 44 241 59 777.6 0 59 242 43 777.6 0 43 243 47 777.6 0 47 244 50 777.6 0 50 245 75 777.6 0 75 246 77 777.6 0 77 247 108 777.6 0 108 248 71 777.6 0 71 249 105 777.6 0 105 250 106 777.6 0 106 251 49 777.6 0 49 252 71 777.6 0 71 253 64 777.6 0 64 254 65 777.6 0 65 255 94 777.6 0 94 256 71 777.6 0 71 257 51 777.6 0 51 258 81 777.6 0 81 259 87 777.6 0 87 260 723 777.6 0 723 261 638 777.6 0 638 262 829 777.6 0 829 263 488 777.6 0 488 264 191 777.6 0 191 265 352 777.6 0 352 266 126 777.6 0 126 267 88 777.6 0 88 268 90 777.6 0 90 269 77 777.6 0 77 270 112 777.6 0 112 271 130 777.6 0 130 272 160 777.6 0 160 273 98 777.6 0 98 274 74 777.6 0 74 275 56 777.6 0 56 276 110 777.6 0 110 277 122 777.6 0 122 278 135 777.6 0 135 279 139 777.6 0 139 280 109 777.6 0 109 281 137 777.6 0 137 282 152 777.6 0 152 283 205 777.6 0 205 284 145 777.6 0 145 285 1744 777.6 0 1744 286 436 777.6 0 436 287 1098 777.6 0 1098 288 160 777.6 0 160 289 131 777.6 0 131 290 191 777.6 0 191 291 142 777.6 0 142 292 75 777.6 0 75 293 30 777.6 0 30 294 100 777.6 0 100 295 99 777.6 0 99 296 209 777.6 0 209 297 182 777.6 0 182 298 154 777.6 0 154 299 85 777.6 0 85 300 58 777.6 0 58 301 40 777.6 0 40 Finished in 4.66 s (63 us/read; 0.95 M reads/minute). === Summary === Total reads processed: 73,688 Reads with adapters: 5,163 (7.0%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 5,155 (7.0%) Total basepairs processed: 20,248,677 bp Total written (filtered): 1,326,184 bp (6.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5163 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 14 0.0 3 0 13 0 1 20 3480 0.0 4 3422 54 2 2 21 10 0.0 4 1 7 0 0 2 22 9 0.0 4 2 5 1 0 1 23 864 0.0 4 849 12 0 3 24 1 0.0 4 0 1 40 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 87 2 0.0 4 1 0 0 0 1 106 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 769 0.0 4 0 0 1 0 768 128 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 5,155 Reads with adapters: 43 (0.8%) Reads written (passing filters): 5,155 (100.0%) Total basepairs processed: 1,326,184 bp Total written (filtered): 1,322,477 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 43 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.7% C: 4.7% G: 88.4% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 80.5 0 2 5 1 5.0 0 1 10 5 0.0 1 0 5 11 22 0.0 1 0 22 238 2 0.0 1 2 260 1 0.0 1 1 262 1 0.0 1 1 267 8 0.0 1 7 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.