This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.07 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 111,347 (3.5%) Reads that were too short: 38,749 (1.2%) Reads written (passing filters): 72,598 (2.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 13,122,660 bp (1.4%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 111347 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5986 777.6 0 5986 7 492 777.6 0 492 8 15508 777.6 0 15508 9 50 777.6 0 50 10 7 777.6 0 7 11 7 777.6 0 7 12 4 777.6 0 4 13 5 777.6 0 5 14 8 777.6 0 8 15 5 777.6 0 5 16 6 777.6 0 6 17 12 777.6 0 12 18 13 777.6 0 13 19 8 777.6 0 8 20 5 777.6 0 5 21 35 777.6 0 35 22 6 777.6 0 6 23 24 777.6 0 24 24 15 777.6 0 15 25 11 777.6 0 11 26 480 777.6 0 480 27 41 777.6 0 41 28 11 777.6 0 11 29 15 777.6 0 15 30 11 777.6 0 11 31 9 777.6 0 9 32 19 777.6 0 19 33 23 777.6 0 23 34 28 777.6 0 28 35 17 777.6 0 17 36 13 777.6 0 13 37 9 777.6 0 9 38 6 777.6 0 6 39 4 777.6 0 4 40 10 777.6 0 10 41 8 777.6 0 8 42 12 777.6 0 12 43 11 777.6 0 11 44 14 777.6 0 14 45 5 777.6 0 5 46 8 777.6 0 8 47 8 777.6 0 8 48 12 777.6 0 12 49 5 777.6 0 5 50 9 777.6 0 9 51 10 777.6 0 10 52 6 777.6 0 6 53 18 777.6 0 18 54 34 777.6 0 34 55 13 777.6 0 13 56 10 777.6 0 10 57 14 777.6 0 14 58 9 777.6 0 9 59 18 777.6 0 18 60 144 777.6 0 144 61 8 777.6 0 8 62 12 777.6 0 12 63 13 777.6 0 13 64 13 777.6 0 13 65 20 777.6 0 20 66 16 777.6 0 16 67 15 777.6 0 15 68 30 777.6 0 30 69 18 777.6 0 18 70 115 777.6 0 115 71 25 777.6 0 25 72 4 777.6 0 4 73 11 777.6 0 11 74 9 777.6 0 9 75 13 777.6 0 13 76 24 777.6 0 24 77 14 777.6 0 14 78 17 777.6 0 17 79 88 777.6 0 88 80 105 777.6 0 105 81 127 777.6 0 127 82 78 777.6 0 78 83 33 777.6 0 33 84 45 777.6 0 45 85 26 777.6 0 26 86 65 777.6 0 65 87 146 777.6 0 146 88 26 777.6 0 26 89 43 777.6 0 43 90 50 777.6 0 50 91 18 777.6 0 18 92 30 777.6 0 30 93 14 777.6 0 14 94 13 777.6 0 13 95 42 777.6 0 42 96 50 777.6 0 50 97 253 777.6 0 253 98 29 777.6 0 29 99 27 777.6 0 27 100 23 777.6 0 23 101 37 777.6 0 37 102 28 777.6 0 28 103 38 777.6 0 38 104 29 777.6 0 29 105 8 777.6 0 8 106 16 777.6 0 16 107 38 777.6 0 38 108 27 777.6 0 27 109 60 777.6 0 60 110 14 777.6 0 14 111 14 777.6 0 14 112 36 777.6 0 36 113 41 777.6 0 41 114 45 777.6 0 45 115 56 777.6 0 56 116 53 777.6 0 53 117 110 777.6 0 110 118 159 777.6 0 159 119 135 777.6 0 135 120 33 777.6 0 33 121 29 777.6 0 29 122 30 777.6 0 30 123 38 777.6 0 38 124 25 777.6 0 25 125 27 777.6 0 27 126 22 777.6 0 22 127 38 777.6 0 38 128 63 777.6 0 63 129 58 777.6 0 58 130 45 777.6 0 45 131 115 777.6 0 115 132 109 777.6 0 109 133 186 777.6 0 186 134 69 777.6 0 69 135 64 777.6 0 64 136 55 777.6 0 55 137 40 777.6 0 40 138 86 777.6 0 86 139 40 777.6 0 40 140 68 777.6 0 68 141 138 777.6 0 138 142 69 777.6 0 69 143 45 777.6 0 45 144 18 777.6 0 18 145 29 777.6 0 29 146 44 777.6 0 44 147 83 777.6 0 83 148 68 777.6 0 68 149 48 777.6 0 48 150 45 777.6 0 45 151 34 777.6 0 34 152 30 777.6 0 30 153 36 777.6 0 36 154 50 777.6 0 50 155 59 777.6 0 59 156 118 777.6 0 118 157 48 777.6 0 48 158 88 777.6 0 88 159 59 777.6 0 59 160 94 777.6 0 94 161 127 777.6 0 127 162 96 777.6 0 96 163 117 777.6 0 117 164 210 777.6 0 210 165 326 777.6 0 326 166 183 777.6 0 183 167 229 777.6 0 229 168 267 777.6 0 267 169 130 777.6 0 130 170 171 777.6 0 171 171 180 777.6 0 180 172 165 777.6 0 165 173 244 777.6 0 244 174 216 777.6 0 216 175 302 777.6 0 302 176 907 777.6 0 907 177 7698 777.6 0 7698 178 16654 777.6 0 16654 179 2297 777.6 0 2297 180 6694 777.6 0 6694 181 1577 777.6 0 1577 182 1471 777.6 0 1471 183 400 777.6 0 400 184 72 777.6 0 72 185 88 777.6 0 88 186 77 777.6 0 77 187 109 777.6 0 109 188 109 777.6 0 109 189 52 777.6 0 52 190 24 777.6 0 24 191 52 777.6 0 52 192 35 777.6 0 35 193 68 777.6 0 68 194 139 777.6 0 139 195 391 777.6 0 391 196 541 777.6 0 541 197 485 777.6 0 485 198 516 777.6 0 516 199 123 777.6 0 123 200 459 777.6 0 459 201 231 777.6 0 231 202 201 777.6 0 201 203 224 777.6 0 224 204 68 777.6 0 68 205 19 777.6 0 19 206 48 777.6 0 48 207 51 777.6 0 51 208 30 777.6 0 30 209 25 777.6 0 25 210 25 777.6 0 25 211 24 777.6 0 24 212 33 777.6 0 33 213 46 777.6 0 46 214 58 777.6 0 58 215 167 777.6 0 167 216 68 777.6 0 68 217 137 777.6 0 137 218 53 777.6 0 53 219 47 777.6 0 47 220 102 777.6 0 102 221 55 777.6 0 55 222 63 777.6 0 63 223 138 777.6 0 138 224 186 777.6 0 186 225 165 777.6 0 165 226 88 777.6 0 88 227 62 777.6 0 62 228 60 777.6 0 60 229 81 777.6 0 81 230 51 777.6 0 51 231 76 777.6 0 76 232 70 777.6 0 70 233 24 777.6 0 24 234 189 777.6 0 189 235 130 777.6 0 130 236 409 777.6 0 409 237 189 777.6 0 189 238 104 777.6 0 104 239 755 777.6 0 755 240 509 777.6 0 509 241 870 777.6 0 870 242 299 777.6 0 299 243 148 777.6 0 148 244 35 777.6 0 35 245 56 777.6 0 56 246 105 777.6 0 105 247 74 777.6 0 74 248 100 777.6 0 100 249 114 777.6 0 114 250 268 777.6 0 268 251 63 777.6 0 63 252 57 777.6 0 57 253 82 777.6 0 82 254 145 777.6 0 145 255 122 777.6 0 122 256 93 777.6 0 93 257 168 777.6 0 168 258 203 777.6 0 203 259 812 777.6 0 812 260 1572 777.6 0 1572 261 2164 777.6 0 2164 262 683 777.6 0 683 263 342 777.6 0 342 264 773 777.6 0 773 265 837 777.6 0 837 266 956 777.6 0 956 267 366 777.6 0 366 268 84 777.6 0 84 269 62 777.6 0 62 270 48 777.6 0 48 271 31 777.6 0 31 272 17 777.6 0 17 273 44 777.6 0 44 274 84 777.6 0 84 275 169 777.6 0 169 276 101 777.6 0 101 277 71 777.6 0 71 278 111 777.6 0 111 279 42 777.6 0 42 280 144 777.6 0 144 281 192 777.6 0 192 282 92 777.6 0 92 283 77 777.6 0 77 284 69 777.6 0 69 285 69 777.6 0 69 286 256 777.6 0 256 287 193 777.6 0 193 288 465 777.6 0 465 289 327 777.6 0 327 290 260 777.6 0 260 291 1631 777.6 0 1631 292 4021 777.6 0 4021 293 11340 777.6 0 11340 294 523 777.6 0 523 295 336 777.6 0 336 296 226 777.6 0 226 297 285 777.6 0 285 298 191 777.6 0 191 299 95 777.6 0 95 300 571 777.6 0 571 301 185 777.6 0 185 Finished in 2.96 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 72,598 Reads with adapters: 17,108 (23.6%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 17,077 (23.5%) Total basepairs processed: 13,122,660 bp Total written (filtered): 4,703,613 bp (35.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17108 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 3 2 16 110 0.0 3 0 95 11 4 17 10846 0.0 3 10481 330 27 8 18 32 0.0 3 2 22 7 1 19 112 0.0 3 7 55 37 13 20 5916 0.0 3 5658 208 20 30 21 17 0.0 3 3 13 0 1 22 6 0.0 3 0 0 3 3 23 9 0.0 3 0 0 0 9 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 60 6 0.0 3 0 0 0 6 63 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 4 0.0 3 0 0 0 4 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 112 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 1 125 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 1 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.76 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 17,077 Reads with adapters: 534 (3.1%) Reads written (passing filters): 17,077 (100.0%) Total basepairs processed: 4,703,613 bp Total written (filtered): 4,701,897 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 534 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.7% C: 11.0% G: 30.1% T: 19.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 422 266.8 0 422 4 110 66.7 0 110 5 2 16.7 0 2