This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.31 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 102,882 (3.2%) Reads that were too short: 10,884 (0.3%) Reads written (passing filters): 91,998 (2.9%) Total basepairs processed: 958,672,960 bp Total written (filtered): 22,752,031 bp (2.4%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 102882 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 299 777.6 0 299 7 771 777.6 0 771 8 39291 777.6 0 39291 9 10467 777.6 0 10467 10 17 777.6 0 17 11 5 777.6 0 5 12 10 777.6 0 10 13 8 777.6 0 8 14 4 777.6 0 4 15 7 777.6 0 7 16 5 777.6 0 5 17 4 777.6 0 4 18 7 777.6 0 7 19 5 777.6 0 5 20 7 777.6 0 7 21 8 777.6 0 8 22 8 777.6 0 8 23 8 777.6 0 8 24 4 777.6 0 4 25 7 777.6 0 7 26 6 777.6 0 6 27 5 777.6 0 5 28 3 777.6 0 3 29 14 777.6 0 14 30 1 777.6 0 1 31 8 777.6 0 8 32 13 777.6 0 13 33 39 777.6 0 39 34 19 777.6 0 19 35 22 777.6 0 22 36 6 777.6 0 6 37 3 777.6 0 3 38 13 777.6 0 13 39 23 777.6 0 23 40 20 777.6 0 20 41 5 777.6 0 5 42 5 777.6 0 5 43 3 777.6 0 3 44 11 777.6 0 11 45 10 777.6 0 10 46 13 777.6 0 13 47 6 777.6 0 6 48 14 777.6 0 14 49 6 777.6 0 6 50 11 777.6 0 11 51 12 777.6 0 12 52 36 777.6 0 36 53 19 777.6 0 19 54 67 777.6 0 67 55 8 777.6 0 8 56 17 777.6 0 17 57 65 777.6 0 65 58 7 777.6 0 7 59 11 777.6 0 11 60 8 777.6 0 8 61 1016 777.6 0 1016 62 73 777.6 0 73 63 62 777.6 0 62 64 18 777.6 0 18 65 35 777.6 0 35 66 19 777.6 0 19 67 40 777.6 0 40 68 10 777.6 0 10 69 11 777.6 0 11 70 11 777.6 0 11 71 30 777.6 0 30 72 22 777.6 0 22 73 6 777.6 0 6 74 8 777.6 0 8 75 20 777.6 0 20 76 47 777.6 0 47 77 27 777.6 0 27 78 18 777.6 0 18 79 14 777.6 0 14 80 12 777.6 0 12 81 18 777.6 0 18 82 75 777.6 0 75 83 36 777.6 0 36 84 53 777.6 0 53 85 92 777.6 0 92 86 597 777.6 0 597 87 224 777.6 0 224 88 233 777.6 0 233 89 89 777.6 0 89 90 106 777.6 0 106 91 27 777.6 0 27 92 31 777.6 0 31 93 48 777.6 0 48 94 64 777.6 0 64 95 34 777.6 0 34 96 34 777.6 0 34 97 40 777.6 0 40 98 90 777.6 0 90 99 82 777.6 0 82 100 174 777.6 0 174 101 344 777.6 0 344 102 592 777.6 0 592 103 9284 777.6 0 9284 104 13289 777.6 0 13289 105 5309 777.6 0 5309 106 276 777.6 0 276 107 124 777.6 0 124 108 193 777.6 0 193 109 18 777.6 0 18 110 18 777.6 0 18 111 36 777.6 0 36 112 33 777.6 0 33 113 23 777.6 0 23 114 16 777.6 0 16 115 21 777.6 0 21 116 12 777.6 0 12 117 4 777.6 0 4 118 26 777.6 0 26 119 159 777.6 0 159 120 78 777.6 0 78 121 70 777.6 0 70 122 32 777.6 0 32 123 27 777.6 0 27 124 39 777.6 0 39 125 142 777.6 0 142 126 170 777.6 0 170 127 40 777.6 0 40 128 52 777.6 0 52 129 33 777.6 0 33 130 24 777.6 0 24 131 20 777.6 0 20 132 69 777.6 0 69 133 197 777.6 0 197 134 246 777.6 0 246 135 74 777.6 0 74 136 61 777.6 0 61 137 70 777.6 0 70 138 107 777.6 0 107 139 523 777.6 0 523 140 178 777.6 0 178 141 98 777.6 0 98 142 33 777.6 0 33 143 103 777.6 0 103 144 120 777.6 0 120 145 187 777.6 0 187 146 38 777.6 0 38 147 36 777.6 0 36 148 32 777.6 0 32 149 94 777.6 0 94 150 316 777.6 0 316 151 87 777.6 0 87 152 65 777.6 0 65 153 111 777.6 0 111 154 65 777.6 0 65 155 62 777.6 0 62 156 59 777.6 0 59 157 64 777.6 0 64 158 66 777.6 0 66 159 185 777.6 0 185 160 142 777.6 0 142 161 64 777.6 0 64 162 29 777.6 0 29 163 43 777.6 0 43 164 98 777.6 0 98 165 129 777.6 0 129 166 60 777.6 0 60 167 40 777.6 0 40 168 53 777.6 0 53 169 40 777.6 0 40 170 39 777.6 0 39 171 119 777.6 0 119 172 311 777.6 0 311 173 144 777.6 0 144 174 34 777.6 0 34 175 11 777.6 0 11 176 19 777.6 0 19 177 18 777.6 0 18 178 93 777.6 0 93 179 63 777.6 0 63 180 139 777.6 0 139 181 60 777.6 0 60 182 57 777.6 0 57 183 65 777.6 0 65 184 163 777.6 0 163 185 178 777.6 0 178 186 67 777.6 0 67 187 24 777.6 0 24 188 23 777.6 0 23 189 179 777.6 0 179 190 124 777.6 0 124 191 113 777.6 0 113 192 24 777.6 0 24 193 13 777.6 0 13 194 18 777.6 0 18 195 13 777.6 0 13 196 20 777.6 0 20 197 25 777.6 0 25 198 24 777.6 0 24 199 22 777.6 0 22 200 58 777.6 0 58 201 73 777.6 0 73 202 54 777.6 0 54 203 25 777.6 0 25 204 41 777.6 0 41 205 220 777.6 0 220 206 380 777.6 0 380 207 363 777.6 0 363 208 191 777.6 0 191 209 68 777.6 0 68 210 81 777.6 0 81 211 140 777.6 0 140 212 126 777.6 0 126 213 133 777.6 0 133 214 122 777.6 0 122 215 141 777.6 0 141 216 167 777.6 0 167 217 78 777.6 0 78 218 118 777.6 0 118 219 211 777.6 0 211 220 212 777.6 0 212 221 104 777.6 0 104 222 49 777.6 0 49 223 129 777.6 0 129 224 276 777.6 0 276 225 318 777.6 0 318 226 170 777.6 0 170 227 195 777.6 0 195 228 103 777.6 0 103 229 68 777.6 0 68 230 46 777.6 0 46 231 72 777.6 0 72 232 25 777.6 0 25 233 42 777.6 0 42 234 55 777.6 0 55 235 136 777.6 0 136 236 45 777.6 0 45 237 25 777.6 0 25 238 15 777.6 0 15 239 16 777.6 0 16 240 27 777.6 0 27 241 32 777.6 0 32 242 33 777.6 0 33 243 20 777.6 0 20 244 50 777.6 0 50 245 60 777.6 0 60 246 75 777.6 0 75 247 47 777.6 0 47 248 24 777.6 0 24 249 20 777.6 0 20 250 23 777.6 0 23 251 42 777.6 0 42 252 31 777.6 0 31 253 58 777.6 0 58 254 285 777.6 0 285 255 689 777.6 0 689 256 189 777.6 0 189 257 101 777.6 0 101 258 130 777.6 0 130 259 497 777.6 0 497 260 448 777.6 0 448 261 562 777.6 0 562 262 379 777.6 0 379 263 411 777.6 0 411 264 110 777.6 0 110 265 214 777.6 0 214 266 163 777.6 0 163 267 70 777.6 0 70 268 26 777.6 0 26 269 9 777.6 0 9 270 15 777.6 0 15 271 9 777.6 0 9 272 11 777.6 0 11 273 32 777.6 0 32 274 84 777.6 0 84 275 38 777.6 0 38 276 43 777.6 0 43 277 42 777.6 0 42 278 104 777.6 0 104 279 99 777.6 0 99 280 109 777.6 0 109 281 100 777.6 0 100 282 47 777.6 0 47 283 58 777.6 0 58 284 65 777.6 0 65 285 61 777.6 0 61 286 95 777.6 0 95 287 32 777.6 0 32 288 20 777.6 0 20 289 5 777.6 0 5 290 10 777.6 0 10 291 11 777.6 0 11 292 9 777.6 0 9 293 10 777.6 0 10 294 18 777.6 0 18 295 12 777.6 0 12 296 8 777.6 0 8 297 7 777.6 0 7 298 13 777.6 0 13 299 16 777.6 0 16 300 18 777.6 0 18 301 98 777.6 0 98 Finished in 5.20 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 91,998 Reads with adapters: 10,997 (12.0%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 10,993 (11.9%) Total basepairs processed: 22,752,031 bp Total written (filtered): 2,978,490 bp (13.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10997 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 36 0.0 3 0 35 0 1 20 10807 0.0 4 10599 191 11 2 4 21 33 0.0 4 6 26 0 0 1 24 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 127 109 0.0 4 0 0 0 0 109 152 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 218 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.46 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 10,993 Reads with adapters: 158 (1.4%) Reads written (passing filters): 10,993 (100.0%) Total basepairs processed: 2,978,490 bp Total written (filtered): 2,976,620 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 158 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 3.2% G: 91.8% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 171.8 0 1 6 3 2.7 0 3 9 19 0.0 0 0 19 10 73 0.0 1 4 69 11 60 0.0 1 0 60 138 1 0.0 1 1 150 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.