This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.16 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 136,628 (4.3%) Reads that were too short: 111,510 (3.5%) Reads written (passing filters): 25,118 (0.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 5,719,029 bp (0.6%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 136628 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 76 777.6 0 76 7 2407 777.6 0 2407 8 128 777.6 0 128 9 15 777.6 0 15 10 15 777.6 0 15 11 12 777.6 0 12 12 12 777.6 0 12 13 14 777.6 0 14 14 16 777.6 0 16 15 18 777.6 0 18 16 36 777.6 0 36 17 25 777.6 0 25 18 20 777.6 0 20 19 45 777.6 0 45 20 93 777.6 0 93 21 30 777.6 0 30 22 16 777.6 0 16 23 14 777.6 0 14 24 12 777.6 0 12 25 18 777.6 0 18 26 6 777.6 0 6 27 11 777.6 0 11 28 24 777.6 0 24 29 29 777.6 0 29 30 34 777.6 0 34 31 15 777.6 0 15 32 15 777.6 0 15 33 21 777.6 0 21 34 17 777.6 0 17 35 16 777.6 0 16 36 11 777.6 0 11 37 17 777.6 0 17 38 17 777.6 0 17 39 49 777.6 0 49 40 7 777.6 0 7 41 16 777.6 0 16 42 15 777.6 0 15 43 12 777.6 0 12 44 19 777.6 0 19 45 12 777.6 0 12 46 22 777.6 0 22 47 13 777.6 0 13 48 29 777.6 0 29 49 35 777.6 0 35 50 30 777.6 0 30 51 48 777.6 0 48 52 19 777.6 0 19 53 17 777.6 0 17 54 23 777.6 0 23 55 37 777.6 0 37 56 39 777.6 0 39 57 22 777.6 0 22 58 36 777.6 0 36 59 62 777.6 0 62 60 104 777.6 0 104 61 199 777.6 0 199 62 342 777.6 0 342 63 68 777.6 0 68 64 86 777.6 0 86 65 197 777.6 0 197 66 74 777.6 0 74 67 76 777.6 0 76 68 146 777.6 0 146 69 77 777.6 0 77 70 166 777.6 0 166 71 128 777.6 0 128 72 170 777.6 0 170 73 159 777.6 0 159 74 269 777.6 0 269 75 6873 777.6 0 6873 76 3558 777.6 0 3558 77 5987 777.6 0 5987 78 136 777.6 0 136 79 17 777.6 0 17 80 16 777.6 0 16 81 10 777.6 0 10 82 12 777.6 0 12 83 15 777.6 0 15 84 8 777.6 0 8 85 10 777.6 0 10 86 4 777.6 0 4 87 13 777.6 0 13 88 15 777.6 0 15 89 3 777.6 0 3 90 10 777.6 0 10 91 15 777.6 0 15 92 7 777.6 0 7 93 9 777.6 0 9 94 6 777.6 0 6 95 12 777.6 0 12 96 11 777.6 0 11 97 5 777.6 0 5 98 5 777.6 0 5 99 15 777.6 0 15 100 6 777.6 0 6 101 7 777.6 0 7 102 5 777.6 0 5 103 11 777.6 0 11 104 8 777.6 0 8 105 12 777.6 0 12 106 11 777.6 0 11 107 7 777.6 0 7 108 10 777.6 0 10 109 2 777.6 0 2 110 9 777.6 0 9 111 9 777.6 0 9 112 8 777.6 0 8 113 5 777.6 0 5 114 11 777.6 0 11 115 11 777.6 0 11 116 6 777.6 0 6 117 7 777.6 0 7 118 8 777.6 0 8 119 9 777.6 0 9 120 14 777.6 0 14 121 18 777.6 0 18 122 8 777.6 0 8 123 10 777.6 0 10 124 15 777.6 0 15 125 164 777.6 0 164 126 117 777.6 0 117 127 76 777.6 0 76 128 22 777.6 0 22 129 56 777.6 0 56 130 21 777.6 0 21 131 6 777.6 0 6 132 16 777.6 0 16 133 12 777.6 0 12 134 16 777.6 0 16 135 33 777.6 0 33 136 33 777.6 0 33 137 55 777.6 0 55 138 51 777.6 0 51 139 79 777.6 0 79 140 24 777.6 0 24 141 38 777.6 0 38 142 21 777.6 0 21 143 15 777.6 0 15 144 21 777.6 0 21 145 52 777.6 0 52 146 11 777.6 0 11 147 6 777.6 0 6 148 8 777.6 0 8 149 9 777.6 0 9 150 3 777.6 0 3 151 2 777.6 0 2 152 5 777.6 0 5 153 7 777.6 0 7 154 4 777.6 0 4 155 9 777.6 0 9 156 6 777.6 0 6 157 11 777.6 0 11 158 5 777.6 0 5 159 12 777.6 0 12 160 11 777.6 0 11 161 11 777.6 0 11 162 21 777.6 0 21 163 30 777.6 0 30 164 14 777.6 0 14 165 20 777.6 0 20 166 20 777.6 0 20 167 46 777.6 0 46 168 46 777.6 0 46 169 14 777.6 0 14 170 13 777.6 0 13 171 9 777.6 0 9 172 7 777.6 0 7 173 6 777.6 0 6 174 5 777.6 0 5 175 9 777.6 0 9 176 3 777.6 0 3 177 4 777.6 0 4 178 12 777.6 0 12 179 10 777.6 0 10 180 21 777.6 0 21 181 20 777.6 0 20 182 23 777.6 0 23 183 27 777.6 0 27 184 17 777.6 0 17 185 9 777.6 0 9 186 29 777.6 0 29 187 14 777.6 0 14 188 29 777.6 0 29 189 12 777.6 0 12 190 6 777.6 0 6 191 14 777.6 0 14 192 22 777.6 0 22 193 117 777.6 0 117 194 77 777.6 0 77 195 48 777.6 0 48 196 14 777.6 0 14 197 21 777.6 0 21 198 64 777.6 0 64 199 90 777.6 0 90 200 10 777.6 0 10 201 20 777.6 0 20 202 17 777.6 0 17 203 79 777.6 0 79 204 147 777.6 0 147 205 60 777.6 0 60 206 21 777.6 0 21 207 22 777.6 0 22 208 34 777.6 0 34 209 16 777.6 0 16 210 34 777.6 0 34 211 76 777.6 0 76 212 40 777.6 0 40 213 39 777.6 0 39 214 38 777.6 0 38 215 112 777.6 0 112 216 37 777.6 0 37 217 83 777.6 0 83 218 87 777.6 0 87 219 180 777.6 0 180 220 41 777.6 0 41 221 75 777.6 0 75 222 56 777.6 0 56 223 433 777.6 0 433 224 274 777.6 0 274 225 339 777.6 0 339 226 80 777.6 0 80 227 51 777.6 0 51 228 79 777.6 0 79 229 174 777.6 0 174 230 119 777.6 0 119 231 128 777.6 0 128 232 200 777.6 0 200 233 358 777.6 0 358 234 474 777.6 0 474 235 745 777.6 0 745 236 1144 777.6 0 1144 237 567 777.6 0 567 238 9532 777.6 0 9532 239 6074 777.6 0 6074 240 5929 777.6 0 5929 241 267 777.6 0 267 242 359 777.6 0 359 243 431 777.6 0 431 244 414 777.6 0 414 245 670 777.6 0 670 246 848 777.6 0 848 247 883 777.6 0 883 248 7470 777.6 0 7470 249 8520 777.6 0 8520 250 56637 777.6 0 56637 251 4047 777.6 0 4047 252 72 777.6 0 72 253 52 777.6 0 52 254 51 777.6 0 51 255 13 777.6 0 13 256 12 777.6 0 12 257 17 777.6 0 17 258 11 777.6 0 11 259 6 777.6 0 6 260 9 777.6 0 9 261 7 777.6 0 7 262 22 777.6 0 22 263 10 777.6 0 10 264 53 777.6 0 53 265 9 777.6 0 9 266 9 777.6 0 9 267 23 777.6 0 23 268 26 777.6 0 26 269 25 777.6 0 25 270 9 777.6 0 9 271 13 777.6 0 13 272 12 777.6 0 12 273 12 777.6 0 12 274 5 777.6 0 5 275 18 777.6 0 18 276 11 777.6 0 11 277 16 777.6 0 16 278 44 777.6 0 44 279 90 777.6 0 90 280 153 777.6 0 153 281 123 777.6 0 123 282 493 777.6 0 493 283 220 777.6 0 220 284 694 777.6 0 694 285 24 777.6 0 24 286 13 777.6 0 13 287 21 777.6 0 21 288 39 777.6 0 39 289 21 777.6 0 21 290 9 777.6 0 9 291 10 777.6 0 10 292 4 777.6 0 4 293 19 777.6 0 19 294 101 777.6 0 101 295 35 777.6 0 35 296 138 777.6 0 138 297 103 777.6 0 103 298 12 777.6 0 12 299 18 777.6 0 18 300 34 777.6 0 34 301 29 777.6 0 29 Finished in 1.22 s (49 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 25,118 Reads with adapters: 2,655 (10.6%) Reads that were too short: 45 (0.2%) Reads written (passing filters): 2,610 (10.4%) Total basepairs processed: 5,719,029 bp Total written (filtered): 686,431 bp (12.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2655 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 1 2 16 24 0.0 3 0 15 3 6 17 2324 0.0 3 2152 129 33 10 18 15 0.0 3 2 11 1 1 19 9 0.0 3 1 1 2 5 20 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 5 0.0 3 0 0 0 5 62 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 91 16 0.0 3 0 0 0 16 92 4 0.0 3 0 0 0 4 93 5 0.0 3 0 0 0 5 94 1 0.0 3 0 0 0 1 96 13 0.0 3 0 0 0 13 98 50 0.0 3 1 0 0 49 99 9 0.0 3 0 0 0 9 100 12 0.0 3 0 0 0 12 101 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 68 0.0 3 0 0 0 68 117 8 0.0 3 0 0 0 8 119 20 0.0 3 0 0 1 19 120 1 0.0 3 0 0 0 1 124 3 0.0 3 0 0 0 3 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 210 1 0.0 3 0 0 0 1 211 3 0.0 3 0 0 0 3 212 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 18 0.0 3 0 0 2 16 226 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 2,610 Reads with adapters: 582 (22.3%) Reads written (passing filters): 2,610 (100.0%) Total basepairs processed: 686,431 bp Total written (filtered): 684,669 bp (99.7%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 582 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.8% C: 72.7% G: 3.4% T: 7.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 567 40.8 0 567 4 14 10.2 0 14 5 1 2.5 0 1