This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 101.37 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 150,565 (4.7%) Reads that were too short: 119,265 (3.7%) Reads written (passing filters): 31,300 (1.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 7,363,369 bp (0.8%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 150565 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 357 777.6 0 357 7 10596 777.6 0 10596 8 2548 777.6 0 2548 9 44 777.6 0 44 10 34 777.6 0 34 11 45 777.6 0 45 12 30 777.6 0 30 13 25 777.6 0 25 14 26 777.6 0 26 15 13 777.6 0 13 16 19 777.6 0 19 17 23 777.6 0 23 18 39 777.6 0 39 19 27 777.6 0 27 20 28 777.6 0 28 21 38 777.6 0 38 22 57 777.6 0 57 23 48 777.6 0 48 24 23 777.6 0 23 25 29 777.6 0 29 26 21 777.6 0 21 27 22 777.6 0 22 28 19 777.6 0 19 29 20 777.6 0 20 30 26 777.6 0 26 31 25 777.6 0 25 32 38 777.6 0 38 33 40 777.6 0 40 34 45 777.6 0 45 35 24 777.6 0 24 36 34 777.6 0 34 37 40 777.6 0 40 38 56 777.6 0 56 39 64 777.6 0 64 40 79 777.6 0 79 41 45 777.6 0 45 42 30 777.6 0 30 43 42 777.6 0 42 44 28 777.6 0 28 45 31 777.6 0 31 46 68 777.6 0 68 47 283 777.6 0 283 48 66 777.6 0 66 49 65 777.6 0 65 50 89 777.6 0 89 51 36 777.6 0 36 52 24 777.6 0 24 53 68 777.6 0 68 54 22 777.6 0 22 55 25 777.6 0 25 56 45 777.6 0 45 57 25 777.6 0 25 58 40 777.6 0 40 59 27 777.6 0 27 60 26 777.6 0 26 61 35 777.6 0 35 62 27 777.6 0 27 63 53 777.6 0 53 64 35 777.6 0 35 65 34 777.6 0 34 66 51 777.6 0 51 67 42 777.6 0 42 68 37 777.6 0 37 69 93 777.6 0 93 70 58 777.6 0 58 71 209 777.6 0 209 72 52 777.6 0 52 73 60 777.6 0 60 74 124 777.6 0 124 75 1533 777.6 0 1533 76 792 777.6 0 792 77 503 777.6 0 503 78 164 777.6 0 164 79 57 777.6 0 57 80 97 777.6 0 97 81 50 777.6 0 50 82 82 777.6 0 82 83 36 777.6 0 36 84 24 777.6 0 24 85 36 777.6 0 36 86 36 777.6 0 36 87 26 777.6 0 26 88 24 777.6 0 24 89 22 777.6 0 22 90 41 777.6 0 41 91 43 777.6 0 43 92 71 777.6 0 71 93 20 777.6 0 20 94 25 777.6 0 25 95 23 777.6 0 23 96 43 777.6 0 43 97 57 777.6 0 57 98 20 777.6 0 20 99 36 777.6 0 36 100 37 777.6 0 37 101 52 777.6 0 52 102 25 777.6 0 25 103 37 777.6 0 37 104 38 777.6 0 38 105 11 777.6 0 11 106 23 777.6 0 23 107 24 777.6 0 24 108 28 777.6 0 28 109 19 777.6 0 19 110 30 777.6 0 30 111 43 777.6 0 43 112 36 777.6 0 36 113 25 777.6 0 25 114 37 777.6 0 37 115 25 777.6 0 25 116 43 777.6 0 43 117 82 777.6 0 82 118 80 777.6 0 80 119 42 777.6 0 42 120 57 777.6 0 57 121 69 777.6 0 69 122 92 777.6 0 92 123 110 777.6 0 110 124 55 777.6 0 55 125 68 777.6 0 68 126 73 777.6 0 73 127 82 777.6 0 82 128 68 777.6 0 68 129 76 777.6 0 76 130 855 777.6 0 855 131 666 777.6 0 666 132 587 777.6 0 587 133 106 777.6 0 106 134 75 777.6 0 75 135 125 777.6 0 125 136 1364 777.6 0 1364 137 1075 777.6 0 1075 138 2411 777.6 0 2411 139 71 777.6 0 71 140 19 777.6 0 19 141 34 777.6 0 34 142 37 777.6 0 37 143 19 777.6 0 19 144 14 777.6 0 14 145 29 777.6 0 29 146 33 777.6 0 33 147 67 777.6 0 67 148 25 777.6 0 25 149 27 777.6 0 27 150 19 777.6 0 19 151 28 777.6 0 28 152 20 777.6 0 20 153 15 777.6 0 15 154 22 777.6 0 22 155 22 777.6 0 22 156 25 777.6 0 25 157 22 777.6 0 22 158 28 777.6 0 28 159 28 777.6 0 28 160 26 777.6 0 26 161 38 777.6 0 38 162 41 777.6 0 41 163 36 777.6 0 36 164 42 777.6 0 42 165 48 777.6 0 48 166 32 777.6 0 32 167 67 777.6 0 67 168 45 777.6 0 45 169 14 777.6 0 14 170 30 777.6 0 30 171 37 777.6 0 37 172 41 777.6 0 41 173 23 777.6 0 23 174 31 777.6 0 31 175 63 777.6 0 63 176 49 777.6 0 49 177 38 777.6 0 38 178 34 777.6 0 34 179 26 777.6 0 26 180 16 777.6 0 16 181 36 777.6 0 36 182 41 777.6 0 41 183 28 777.6 0 28 184 41 777.6 0 41 185 30 777.6 0 30 186 54 777.6 0 54 187 83 777.6 0 83 188 42 777.6 0 42 189 50 777.6 0 50 190 21 777.6 0 21 191 14 777.6 0 14 192 15 777.6 0 15 193 26 777.6 0 26 194 47 777.6 0 47 195 27 777.6 0 27 196 27 777.6 0 27 197 20 777.6 0 20 198 18 777.6 0 18 199 27 777.6 0 27 200 38 777.6 0 38 201 25 777.6 0 25 202 24 777.6 0 24 203 32 777.6 0 32 204 24 777.6 0 24 205 21 777.6 0 21 206 34 777.6 0 34 207 34 777.6 0 34 208 39 777.6 0 39 209 54 777.6 0 54 210 41 777.6 0 41 211 42 777.6 0 42 212 45 777.6 0 45 213 64 777.6 0 64 214 68 777.6 0 68 215 71 777.6 0 71 216 77 777.6 0 77 217 75 777.6 0 75 218 53 777.6 0 53 219 95 777.6 0 95 220 68 777.6 0 68 221 57 777.6 0 57 222 119 777.6 0 119 223 104 777.6 0 104 224 294 777.6 0 294 225 358 777.6 0 358 226 716 777.6 0 716 227 995 777.6 0 995 228 668 777.6 0 668 229 633 777.6 0 633 230 564 777.6 0 564 231 720 777.6 0 720 232 374 777.6 0 374 233 187 777.6 0 187 234 201 777.6 0 201 235 269 777.6 0 269 236 380 777.6 0 380 237 686 777.6 0 686 238 1962 777.6 0 1962 239 662 777.6 0 662 240 1681 777.6 0 1681 241 1687 777.6 0 1687 242 668 777.6 0 668 243 388 777.6 0 388 244 1091 777.6 0 1091 245 765 777.6 0 765 246 1765 777.6 0 1765 247 635 777.6 0 635 248 1484 777.6 0 1484 249 864 777.6 0 864 250 1110 777.6 0 1110 251 14798 777.6 0 14798 252 10086 777.6 0 10086 253 69058 777.6 0 69058 254 398 777.6 0 398 255 40 777.6 0 40 256 52 777.6 0 52 257 44 777.6 0 44 258 30 777.6 0 30 259 16 777.6 0 16 260 25 777.6 0 25 261 27 777.6 0 27 262 49 777.6 0 49 263 52 777.6 0 52 264 57 777.6 0 57 265 41 777.6 0 41 266 30 777.6 0 30 267 61 777.6 0 61 268 137 777.6 0 137 269 25 777.6 0 25 270 24 777.6 0 24 271 18 777.6 0 18 272 12 777.6 0 12 273 34 777.6 0 34 274 22 777.6 0 22 275 25 777.6 0 25 276 25 777.6 0 25 277 35 777.6 0 35 278 25 777.6 0 25 279 20 777.6 0 20 280 60 777.6 0 60 281 29 777.6 0 29 282 29 777.6 0 29 283 35 777.6 0 35 284 38 777.6 0 38 285 58 777.6 0 58 286 38 777.6 0 38 287 45 777.6 0 45 288 36 777.6 0 36 289 25 777.6 0 25 290 43 777.6 0 43 291 40 777.6 0 40 292 51 777.6 0 51 293 57 777.6 0 57 294 42 777.6 0 42 295 72 777.6 0 72 296 39 777.6 0 39 297 38 777.6 0 38 298 59 777.6 0 59 299 73 777.6 0 73 300 30 777.6 0 30 301 14 777.6 0 14 Finished in 1.77 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 31,300 Reads with adapters: 2,680 (8.6%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 2,672 (8.5%) Total basepairs processed: 7,363,369 bp Total written (filtered): 729,119 bp (9.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2680 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 13 0.0 3 0 12 1 20 2639 0.0 4 2599 38 1 0 1 21 10 0.0 4 2 8 22 2 0.0 4 0 0 1 1 23 1 0.0 4 0 0 0 1 24 2 0.0 4 2 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 99 2 0.0 4 0 0 0 0 2 100 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 1 158 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 2,672 Reads with adapters: 59 (2.2%) Reads written (passing filters): 2,672 (100.0%) Total basepairs processed: 729,119 bp Total written (filtered): 728,478 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 59 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 6 1 0.7 0 1 10 3 0.0 1 0 3 11 55 0.0 1 0 55 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.