This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.75 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 632,571 (19.9%) Reads that were too short: 152,536 (4.8%) Reads written (passing filters): 480,035 (15.1%) Total basepairs processed: 958,672,960 bp Total written (filtered): 109,307,409 bp (11.4%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 632571 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1655 777.6 0 1655 7 2283 777.6 0 2283 8 3923 777.6 0 3923 9 239987 777.6 0 239987 10 398 777.6 0 398 11 8 777.6 0 8 12 9 777.6 0 9 13 8 777.6 0 8 14 5 777.6 0 5 15 19 777.6 0 19 16 14 777.6 0 14 17 14 777.6 0 14 18 5 777.6 0 5 19 7 777.6 0 7 20 8 777.6 0 8 21 3 777.6 0 3 22 8 777.6 0 8 23 5 777.6 0 5 24 9 777.6 0 9 25 9 777.6 0 9 26 11 777.6 0 11 27 6 777.6 0 6 28 6 777.6 0 6 29 7 777.6 0 7 30 6 777.6 0 6 31 3 777.6 0 3 32 9 777.6 0 9 33 6 777.6 0 6 34 8 777.6 0 8 35 2 777.6 0 2 36 3 777.6 0 3 37 5 777.6 0 5 38 10 777.6 0 10 39 7 777.6 0 7 40 2 777.6 0 2 41 12 777.6 0 12 42 6 777.6 0 6 43 3 777.6 0 3 44 4 777.6 0 4 45 4 777.6 0 4 46 4 777.6 0 4 47 9 777.6 0 9 48 7 777.6 0 7 49 6 777.6 0 6 50 5 777.6 0 5 51 9 777.6 0 9 52 6 777.6 0 6 53 15 777.6 0 15 54 36 777.6 0 36 55 12 777.6 0 12 56 28 777.6 0 28 57 726 777.6 0 726 58 10 777.6 0 10 59 5 777.6 0 5 60 6 777.6 0 6 61 3 777.6 0 3 62 4 777.6 0 4 63 14 777.6 0 14 64 33 777.6 0 33 65 58 777.6 0 58 66 17 777.6 0 17 67 15 777.6 0 15 68 17 777.6 0 17 69 19 777.6 0 19 70 91 777.6 0 91 71 402 777.6 0 402 72 933 777.6 0 933 73 1379 777.6 0 1379 74 2257 777.6 0 2257 75 879 777.6 0 879 76 656 777.6 0 656 77 1203 777.6 0 1203 78 2830 777.6 0 2830 79 1508 777.6 0 1508 80 1630 777.6 0 1630 81 1508 777.6 0 1508 82 1464 777.6 0 1464 83 1426 777.6 0 1426 84 2275 777.6 0 2275 85 4172 777.6 0 4172 86 1367 777.6 0 1367 87 1812 777.6 0 1812 88 3635 777.6 0 3635 89 1017 777.6 0 1017 90 2970 777.6 0 2970 91 1315 777.6 0 1315 92 938 777.6 0 938 93 1347 777.6 0 1347 94 3080 777.6 0 3080 95 1106 777.6 0 1106 96 891 777.6 0 891 97 448 777.6 0 448 98 516 777.6 0 516 99 377 777.6 0 377 100 2231 777.6 0 2231 101 1466 777.6 0 1466 102 962 777.6 0 962 103 752 777.6 0 752 104 1257 777.6 0 1257 105 834 777.6 0 834 106 1033 777.6 0 1033 107 943 777.6 0 943 108 387 777.6 0 387 109 493 777.6 0 493 110 1963 777.6 0 1963 111 1249 777.6 0 1249 112 1397 777.6 0 1397 113 1267 777.6 0 1267 114 1219 777.6 0 1219 115 738 777.6 0 738 116 1915 777.6 0 1915 117 1206 777.6 0 1206 118 2750 777.6 0 2750 119 1962 777.6 0 1962 120 3420 777.6 0 3420 121 1708 777.6 0 1708 122 1420 777.6 0 1420 123 1534 777.6 0 1534 124 1365 777.6 0 1365 125 1551 777.6 0 1551 126 1345 777.6 0 1345 127 1122 777.6 0 1122 128 1297 777.6 0 1297 129 1153 777.6 0 1153 130 1027 777.6 0 1027 131 2574 777.6 0 2574 132 1340 777.6 0 1340 133 1364 777.6 0 1364 134 1143 777.6 0 1143 135 1143 777.6 0 1143 136 1247 777.6 0 1247 137 1228 777.6 0 1228 138 1320 777.6 0 1320 139 1376 777.6 0 1376 140 1637 777.6 0 1637 141 1252 777.6 0 1252 142 1396 777.6 0 1396 143 1216 777.6 0 1216 144 1282 777.6 0 1282 145 2047 777.6 0 2047 146 1609 777.6 0 1609 147 2054 777.6 0 2054 148 1213 777.6 0 1213 149 1640 777.6 0 1640 150 1184 777.6 0 1184 151 1215 777.6 0 1215 152 2061 777.6 0 2061 153 2295 777.6 0 2295 154 1768 777.6 0 1768 155 1306 777.6 0 1306 156 1090 777.6 0 1090 157 1604 777.6 0 1604 158 1691 777.6 0 1691 159 1209 777.6 0 1209 160 1243 777.6 0 1243 161 995 777.6 0 995 162 1245 777.6 0 1245 163 1264 777.6 0 1264 164 1125 777.6 0 1125 165 1086 777.6 0 1086 166 4104 777.6 0 4104 167 1941 777.6 0 1941 168 2763 777.6 0 2763 169 1550 777.6 0 1550 170 1598 777.6 0 1598 171 4407 777.6 0 4407 172 1537 777.6 0 1537 173 1750 777.6 0 1750 174 1343 777.6 0 1343 175 1337 777.6 0 1337 176 1068 777.6 0 1068 177 1462 777.6 0 1462 178 1774 777.6 0 1774 179 2191 777.6 0 2191 180 9222 777.6 0 9222 181 17584 777.6 0 17584 182 3729 777.6 0 3729 183 6211 777.6 0 6211 184 1981 777.6 0 1981 185 1775 777.6 0 1775 186 1121 777.6 0 1121 187 1190 777.6 0 1190 188 1122 777.6 0 1122 189 1545 777.6 0 1545 190 1303 777.6 0 1303 191 1488 777.6 0 1488 192 1161 777.6 0 1161 193 1240 777.6 0 1240 194 730 777.6 0 730 195 1047 777.6 0 1047 196 1141 777.6 0 1141 197 1391 777.6 0 1391 198 2192 777.6 0 2192 199 2385 777.6 0 2385 200 1797 777.6 0 1797 201 1434 777.6 0 1434 202 993 777.6 0 993 203 1706 777.6 0 1706 204 1517 777.6 0 1517 205 1370 777.6 0 1370 206 1158 777.6 0 1158 207 1091 777.6 0 1091 208 1124 777.6 0 1124 209 957 777.6 0 957 210 1153 777.6 0 1153 211 1069 777.6 0 1069 212 1097 777.6 0 1097 213 2112 777.6 0 2112 214 2805 777.6 0 2805 215 2408 777.6 0 2408 216 1092 777.6 0 1092 217 1136 777.6 0 1136 218 1538 777.6 0 1538 219 1263 777.6 0 1263 220 804 777.6 0 804 221 821 777.6 0 821 222 1126 777.6 0 1126 223 1448 777.6 0 1448 224 1070 777.6 0 1070 225 913 777.6 0 913 226 852 777.6 0 852 227 805 777.6 0 805 228 1066 777.6 0 1066 229 1109 777.6 0 1109 230 981 777.6 0 981 231 1092 777.6 0 1092 232 962 777.6 0 962 233 1382 777.6 0 1382 234 1402 777.6 0 1402 235 1657 777.6 0 1657 236 929 777.6 0 929 237 1304 777.6 0 1304 238 1305 777.6 0 1305 239 1843 777.6 0 1843 240 1561 777.6 0 1561 241 1529 777.6 0 1529 242 1751 777.6 0 1751 243 1533 777.6 0 1533 244 1515 777.6 0 1515 245 847 777.6 0 847 246 1071 777.6 0 1071 247 1018 777.6 0 1018 248 1418 777.6 0 1418 249 1385 777.6 0 1385 250 1364 777.6 0 1364 251 1231 777.6 0 1231 252 1630 777.6 0 1630 253 1438 777.6 0 1438 254 1077 777.6 0 1077 255 527 777.6 0 527 256 520 777.6 0 520 257 780 777.6 0 780 258 826 777.6 0 826 259 906 777.6 0 906 260 1333 777.6 0 1333 261 2397 777.6 0 2397 262 2351 777.6 0 2351 263 2255 777.6 0 2255 264 1703 777.6 0 1703 265 1258 777.6 0 1258 266 1187 777.6 0 1187 267 4316 777.6 0 4316 268 5300 777.6 0 5300 269 13689 777.6 0 13689 270 1669 777.6 0 1669 271 1120 777.6 0 1120 272 1329 777.6 0 1329 273 1150 777.6 0 1150 274 1138 777.6 0 1138 275 905 777.6 0 905 276 1001 777.6 0 1001 277 1462 777.6 0 1462 278 1607 777.6 0 1607 279 1454 777.6 0 1454 280 2282 777.6 0 2282 281 2859 777.6 0 2859 282 1727 777.6 0 1727 283 1116 777.6 0 1116 284 1182 777.6 0 1182 285 910 777.6 0 910 286 1102 777.6 0 1102 287 982 777.6 0 982 288 959 777.6 0 959 289 1083 777.6 0 1083 290 1159 777.6 0 1159 291 1446 777.6 0 1446 292 1309 777.6 0 1309 293 1224 777.6 0 1224 294 1043 777.6 0 1043 295 823 777.6 0 823 296 1059 777.6 0 1059 297 1357 777.6 0 1357 298 1061 777.6 0 1061 299 1841 777.6 0 1841 300 3382 777.6 0 3382 301 1619 777.6 0 1619 Finished in 20.47 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 480,035 Reads with adapters: 247,090 (51.5%) Reads that were too short: 71 (0.0%) Reads written (passing filters): 247,019 (51.5%) Total basepairs processed: 109,307,409 bp Total written (filtered): 67,919,768 bp (62.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 247090 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 19 0.0 2 0 0 0 19 15 86 0.0 3 0 0 77 9 16 2166 0.0 3 0 2049 87 30 17 243773 0.0 3 234809 7900 817 247 18 559 0.0 3 29 477 28 25 19 127 0.0 3 2 1 90 34 20 130 0.0 3 1 0 1 128 21 31 0.0 3 1 0 1 29 23 2 0.0 3 0 0 0 2 24 50 0.0 3 0 0 1 49 25 2 0.0 3 1 0 0 1 27 5 0.0 3 1 0 0 4 28 3 0.0 3 1 0 1 1 30 1 0.0 3 1 33 3 0.0 3 0 0 0 3 34 1 0.0 3 1 35 3 0.0 3 2 0 0 1 36 2 0.0 3 0 0 0 2 37 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 1 1 41 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 0 4 47 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 5 0.0 3 0 0 0 5 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 6 0.0 3 0 0 0 6 72 2 0.0 3 0 0 0 2 77 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 1 121 1 0.0 3 0 0 0 1 122 4 0.0 3 0 0 0 4 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 3 0.0 3 0 0 0 3 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 285 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 11.21 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 247,019 Reads with adapters: 10,215 (4.1%) Reads written (passing filters): 247,019 (100.0%) Total basepairs processed: 67,919,768 bp Total written (filtered): 67,887,563 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 10215 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.1% C: 8.2% G: 37.9% T: 14.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8679 3859.7 0 8679 4 1524 964.9 0 1524 5 4 241.2 0 4 6 7 60.3 0 7 10 1 0.2 1 0 1