This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.19 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 21,906 (0.7%) Reads that were too short: 8,469 (0.3%) Reads written (passing filters): 13,437 (0.4%) Total basepairs processed: 958,672,960 bp Total written (filtered): 2,503,243 bp (0.3%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 21906 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 78 777.6 0 78 7 55 777.6 0 55 8 1680 777.6 0 1680 9 115 777.6 0 115 10 13 777.6 0 13 11 20 777.6 0 20 12 14 777.6 0 14 13 10 777.6 0 10 14 8 777.6 0 8 15 13 777.6 0 13 16 6 777.6 0 6 17 18 777.6 0 18 18 12 777.6 0 12 19 5 777.6 0 5 20 9 777.6 0 9 21 13 777.6 0 13 22 5 777.6 0 5 23 9 777.6 0 9 24 4 777.6 0 4 25 4 777.6 0 4 26 8 777.6 0 8 27 13 777.6 0 13 28 16 777.6 0 16 29 11 777.6 0 11 30 2 777.6 0 2 31 6 777.6 0 6 32 12 777.6 0 12 33 8 777.6 0 8 34 12 777.6 0 12 35 11 777.6 0 11 36 7 777.6 0 7 37 9 777.6 0 9 38 13 777.6 0 13 39 102 777.6 0 102 40 14 777.6 0 14 41 11 777.6 0 11 42 8 777.6 0 8 43 15 777.6 0 15 44 7 777.6 0 7 45 7 777.6 0 7 46 12 777.6 0 12 47 11 777.6 0 11 48 16 777.6 0 16 49 10 777.6 0 10 50 12 777.6 0 12 51 6 777.6 0 6 52 20 777.6 0 20 53 423 777.6 0 423 54 45 777.6 0 45 55 13 777.6 0 13 56 7 777.6 0 7 57 8 777.6 0 8 58 14 777.6 0 14 59 18 777.6 0 18 60 7 777.6 0 7 61 12 777.6 0 12 62 12 777.6 0 12 63 40 777.6 0 40 64 92 777.6 0 92 65 84 777.6 0 84 66 8 777.6 0 8 67 16 777.6 0 16 68 15 777.6 0 15 69 16 777.6 0 16 70 19 777.6 0 19 71 30 777.6 0 30 72 211 777.6 0 211 73 512 777.6 0 512 74 511 777.6 0 511 75 655 777.6 0 655 76 10 777.6 0 10 77 3 777.6 0 3 78 14 777.6 0 14 79 16 777.6 0 16 80 3 777.6 0 3 81 11 777.6 0 11 82 10 777.6 0 10 83 17 777.6 0 17 84 38 777.6 0 38 85 36 777.6 0 36 86 36 777.6 0 36 87 60 777.6 0 60 88 124 777.6 0 124 89 17 777.6 0 17 90 12 777.6 0 12 91 17 777.6 0 17 92 8 777.6 0 8 93 10 777.6 0 10 94 22 777.6 0 22 95 14 777.6 0 14 96 16 777.6 0 16 97 4 777.6 0 4 98 9 777.6 0 9 99 16 777.6 0 16 100 6 777.6 0 6 101 9 777.6 0 9 102 8 777.6 0 8 103 9 777.6 0 9 104 12 777.6 0 12 105 6 777.6 0 6 106 6 777.6 0 6 107 3 777.6 0 3 108 20 777.6 0 20 109 12 777.6 0 12 110 15 777.6 0 15 111 12 777.6 0 12 112 5 777.6 0 5 113 7 777.6 0 7 114 9 777.6 0 9 115 8 777.6 0 8 116 12 777.6 0 12 117 12 777.6 0 12 118 14 777.6 0 14 119 8 777.6 0 8 120 17 777.6 0 17 121 20 777.6 0 20 122 21 777.6 0 21 123 21 777.6 0 21 124 18 777.6 0 18 125 13 777.6 0 13 126 12 777.6 0 12 127 16 777.6 0 16 128 18 777.6 0 18 129 27 777.6 0 27 130 41 777.6 0 41 131 21 777.6 0 21 132 24 777.6 0 24 133 25 777.6 0 25 134 22 777.6 0 22 135 52 777.6 0 52 136 186 777.6 0 186 137 84 777.6 0 84 138 61 777.6 0 61 139 29 777.6 0 29 140 21 777.6 0 21 141 34 777.6 0 34 142 30 777.6 0 30 143 27 777.6 0 27 144 22 777.6 0 22 145 43 777.6 0 43 146 501 777.6 0 501 147 109 777.6 0 109 148 119 777.6 0 119 149 99 777.6 0 99 150 27 777.6 0 27 151 11 777.6 0 11 152 15 777.6 0 15 153 37 777.6 0 37 154 21 777.6 0 21 155 48 777.6 0 48 156 76 777.6 0 76 157 135 777.6 0 135 158 421 777.6 0 421 159 204 777.6 0 204 160 239 777.6 0 239 161 53 777.6 0 53 162 97 777.6 0 97 163 42 777.6 0 42 164 37 777.6 0 37 165 34 777.6 0 34 166 68 777.6 0 68 167 125 777.6 0 125 168 89 777.6 0 89 169 53 777.6 0 53 170 42 777.6 0 42 171 95 777.6 0 95 172 87 777.6 0 87 173 64 777.6 0 64 174 414 777.6 0 414 175 223 777.6 0 223 176 126 777.6 0 126 177 42 777.6 0 42 178 23 777.6 0 23 179 27 777.6 0 27 180 155 777.6 0 155 181 409 777.6 0 409 182 323 777.6 0 323 183 68 777.6 0 68 184 53 777.6 0 53 185 79 777.6 0 79 186 609 777.6 0 609 187 420 777.6 0 420 188 295 777.6 0 295 189 156 777.6 0 156 190 16 777.6 0 16 191 18 777.6 0 18 192 49 777.6 0 49 193 12 777.6 0 12 194 18 777.6 0 18 195 19 777.6 0 19 196 8 777.6 0 8 197 9 777.6 0 9 198 9 777.6 0 9 199 17 777.6 0 17 200 17 777.6 0 17 201 16 777.6 0 16 202 35 777.6 0 35 203 29 777.6 0 29 204 93 777.6 0 93 205 75 777.6 0 75 206 32 777.6 0 32 207 37 777.6 0 37 208 26 777.6 0 26 209 21 777.6 0 21 210 20 777.6 0 20 211 114 777.6 0 114 212 22 777.6 0 22 213 18 777.6 0 18 214 33 777.6 0 33 215 29 777.6 0 29 216 24 777.6 0 24 217 30 777.6 0 30 218 22 777.6 0 22 219 17 777.6 0 17 220 15 777.6 0 15 221 22 777.6 0 22 222 39 777.6 0 39 223 20 777.6 0 20 224 21 777.6 0 21 225 34 777.6 0 34 226 76 777.6 0 76 227 193 777.6 0 193 228 78 777.6 0 78 229 94 777.6 0 94 230 13 777.6 0 13 231 23 777.6 0 23 232 16 777.6 0 16 233 21 777.6 0 21 234 14 777.6 0 14 235 11 777.6 0 11 236 18 777.6 0 18 237 36 777.6 0 36 238 30 777.6 0 30 239 9 777.6 0 9 240 15 777.6 0 15 241 20 777.6 0 20 242 36 777.6 0 36 243 18 777.6 0 18 244 22 777.6 0 22 245 14 777.6 0 14 246 44 777.6 0 44 247 97 777.6 0 97 248 86 777.6 0 86 249 78 777.6 0 78 250 178 777.6 0 178 251 1623 777.6 0 1623 252 662 777.6 0 662 253 1987 777.6 0 1987 254 188 777.6 0 188 255 86 777.6 0 86 256 54 777.6 0 54 257 89 777.6 0 89 258 41 777.6 0 41 259 30 777.6 0 30 260 21 777.6 0 21 261 35 777.6 0 35 262 32 777.6 0 32 263 89 777.6 0 89 264 59 777.6 0 59 265 36 777.6 0 36 266 25 777.6 0 25 267 43 777.6 0 43 268 25 777.6 0 25 269 39 777.6 0 39 270 18 777.6 0 18 271 15 777.6 0 15 272 13 777.6 0 13 273 16 777.6 0 16 274 18 777.6 0 18 275 14 777.6 0 14 276 17 777.6 0 17 277 20 777.6 0 20 278 30 777.6 0 30 279 36 777.6 0 36 280 41 777.6 0 41 281 61 777.6 0 61 282 126 777.6 0 126 283 32 777.6 0 32 284 19 777.6 0 19 285 42 777.6 0 42 286 26 777.6 0 26 287 42 777.6 0 42 288 20 777.6 0 20 289 14 777.6 0 14 290 16 777.6 0 16 291 48 777.6 0 48 292 42 777.6 0 42 293 49 777.6 0 49 294 31 777.6 0 31 295 66 777.6 0 66 296 23 777.6 0 23 297 29 777.6 0 29 298 25 777.6 0 25 299 142 777.6 0 142 300 116 777.6 0 116 301 30 777.6 0 30 Finished in 0.60 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 13,437 Reads with adapters: 1,733 (12.9%) Reads that were too short: 21 (0.2%) Reads written (passing filters): 1,712 (12.7%) Total basepairs processed: 2,503,243 bp Total written (filtered): 469,565 bp (18.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1733 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 21 0.0 3 0 17 1 3 17 1651 0.0 3 1604 43 3 1 18 9 0.0 3 2 6 1 19 11 0.0 3 0 0 8 3 20 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 37 1 0.0 3 1 38 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 1 49 4 0.0 3 0 0 0 4 51 1 0.0 3 0 0 0 1 52 1 0.0 3 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 106 7 0.0 3 0 0 0 7 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 1,712 Reads with adapters: 58 (3.4%) Reads written (passing filters): 1,712 (100.0%) Total basepairs processed: 469,565 bp Total written (filtered): 469,384 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 58 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.0% C: 17.2% G: 25.9% T: 25.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 53 26.8 0 53 4 3 6.7 0 3 5 2 1.7 0 2