This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 104.91 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 410,744 (12.9%) Reads that were too short: 20,393 (0.6%) Reads written (passing filters): 390,351 (12.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 84,580,494 bp (8.8%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 410744 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2299 777.6 0 2299 7 164468 777.6 0 164468 8 228 777.6 0 228 9 1542 777.6 0 1542 10 37 777.6 0 37 11 10 777.6 0 10 12 11 777.6 0 11 13 18 777.6 0 18 14 30 777.6 0 30 15 36 777.6 0 36 16 13 777.6 0 13 17 12 777.6 0 12 18 12 777.6 0 12 19 10 777.6 0 10 20 10 777.6 0 10 21 14 777.6 0 14 22 7 777.6 0 7 23 10 777.6 0 10 24 25 777.6 0 25 25 18 777.6 0 18 26 24 777.6 0 24 27 73 777.6 0 73 28 172 777.6 0 172 29 61 777.6 0 61 30 10 777.6 0 10 31 14 777.6 0 14 32 10 777.6 0 10 33 13 777.6 0 13 34 11 777.6 0 11 35 19 777.6 0 19 36 136 777.6 0 136 37 24 777.6 0 24 38 11 777.6 0 11 39 15 777.6 0 15 40 10 777.6 0 10 41 21 777.6 0 21 42 12 777.6 0 12 43 21 777.6 0 21 44 11 777.6 0 11 45 14 777.6 0 14 46 21 777.6 0 21 47 14 777.6 0 14 48 15 777.6 0 15 49 20 777.6 0 20 50 30 777.6 0 30 51 13 777.6 0 13 52 11 777.6 0 11 53 11 777.6 0 11 54 13 777.6 0 13 55 10 777.6 0 10 56 8 777.6 0 8 57 28 777.6 0 28 58 85 777.6 0 85 59 121 777.6 0 121 60 15 777.6 0 15 61 14 777.6 0 14 62 8 777.6 0 8 63 14 777.6 0 14 64 16 777.6 0 16 65 16 777.6 0 16 66 12 777.6 0 12 67 15 777.6 0 15 68 7 777.6 0 7 69 12 777.6 0 12 70 19 777.6 0 19 71 18 777.6 0 18 72 13 777.6 0 13 73 18 777.6 0 18 74 18 777.6 0 18 75 12 777.6 0 12 76 15 777.6 0 15 77 16 777.6 0 16 78 30 777.6 0 30 79 27 777.6 0 27 80 28 777.6 0 28 81 158 777.6 0 158 82 36 777.6 0 36 83 43 777.6 0 43 84 30 777.6 0 30 85 45 777.6 0 45 86 48 777.6 0 48 87 275 777.6 0 275 88 67 777.6 0 67 89 62 777.6 0 62 90 290 777.6 0 290 91 572 777.6 0 572 92 241 777.6 0 241 93 59 777.6 0 59 94 42 777.6 0 42 95 123 777.6 0 123 96 515 777.6 0 515 97 821 777.6 0 821 98 202 777.6 0 202 99 36 777.6 0 36 100 62 777.6 0 62 101 65 777.6 0 65 102 164 777.6 0 164 103 289 777.6 0 289 104 354 777.6 0 354 105 432 777.6 0 432 106 727 777.6 0 727 107 2319 777.6 0 2319 108 24452 777.6 0 24452 109 9420 777.6 0 9420 110 18770 777.6 0 18770 111 129 777.6 0 129 112 66 777.6 0 66 113 78 777.6 0 78 114 58 777.6 0 58 115 75 777.6 0 75 116 50 777.6 0 50 117 122 777.6 0 122 118 242 777.6 0 242 119 103 777.6 0 103 120 145 777.6 0 145 121 170 777.6 0 170 122 112 777.6 0 112 123 97 777.6 0 97 124 127 777.6 0 127 125 119 777.6 0 119 126 87 777.6 0 87 127 281 777.6 0 281 128 441 777.6 0 441 129 189 777.6 0 189 130 99 777.6 0 99 131 141 777.6 0 141 132 162 777.6 0 162 133 445 777.6 0 445 134 8344 777.6 0 8344 135 1403 777.6 0 1403 136 2293 777.6 0 2293 137 212 777.6 0 212 138 272 777.6 0 272 139 137 777.6 0 137 140 239 777.6 0 239 141 325 777.6 0 325 142 390 777.6 0 390 143 310 777.6 0 310 144 388 777.6 0 388 145 365 777.6 0 365 146 722 777.6 0 722 147 1516 777.6 0 1516 148 485 777.6 0 485 149 741 777.6 0 741 150 765 777.6 0 765 151 626 777.6 0 626 152 693 777.6 0 693 153 1012 777.6 0 1012 154 2281 777.6 0 2281 155 9417 777.6 0 9417 156 3291 777.6 0 3291 157 3809 777.6 0 3809 158 1249 777.6 0 1249 159 1741 777.6 0 1741 160 25155 777.6 0 25155 161 42482 777.6 0 42482 162 38321 777.6 0 38321 163 299 777.6 0 299 164 247 777.6 0 247 165 166 777.6 0 166 166 316 777.6 0 316 167 228 777.6 0 228 168 118 777.6 0 118 169 70 777.6 0 70 170 105 777.6 0 105 171 186 777.6 0 186 172 62 777.6 0 62 173 66 777.6 0 66 174 224 777.6 0 224 175 573 777.6 0 573 176 358 777.6 0 358 177 269 777.6 0 269 178 100 777.6 0 100 179 68 777.6 0 68 180 106 777.6 0 106 181 127 777.6 0 127 182 238 777.6 0 238 183 274 777.6 0 274 184 193 777.6 0 193 185 91 777.6 0 91 186 138 777.6 0 138 187 133 777.6 0 133 188 152 777.6 0 152 189 64 777.6 0 64 190 50 777.6 0 50 191 101 777.6 0 101 192 229 777.6 0 229 193 289 777.6 0 289 194 309 777.6 0 309 195 253 777.6 0 253 196 43 777.6 0 43 197 47 777.6 0 47 198 53 777.6 0 53 199 204 777.6 0 204 200 235 777.6 0 235 201 91 777.6 0 91 202 122 777.6 0 122 203 139 777.6 0 139 204 213 777.6 0 213 205 146 777.6 0 146 206 193 777.6 0 193 207 240 777.6 0 240 208 186 777.6 0 186 209 153 777.6 0 153 210 84 777.6 0 84 211 71 777.6 0 71 212 88 777.6 0 88 213 74 777.6 0 74 214 116 777.6 0 116 215 206 777.6 0 206 216 155 777.6 0 155 217 151 777.6 0 151 218 219 777.6 0 219 219 217 777.6 0 217 220 560 777.6 0 560 221 318 777.6 0 318 222 553 777.6 0 553 223 267 777.6 0 267 224 74 777.6 0 74 225 116 777.6 0 116 226 164 777.6 0 164 227 180 777.6 0 180 228 185 777.6 0 185 229 256 777.6 0 256 230 283 777.6 0 283 231 526 777.6 0 526 232 383 777.6 0 383 233 183 777.6 0 183 234 306 777.6 0 306 235 367 777.6 0 367 236 412 777.6 0 412 237 193 777.6 0 193 238 86 777.6 0 86 239 65 777.6 0 65 240 82 777.6 0 82 241 97 777.6 0 97 242 51 777.6 0 51 243 66 777.6 0 66 244 57 777.6 0 57 245 67 777.6 0 67 246 109 777.6 0 109 247 147 777.6 0 147 248 314 777.6 0 314 249 156 777.6 0 156 250 61 777.6 0 61 251 102 777.6 0 102 252 214 777.6 0 214 253 208 777.6 0 208 254 366 777.6 0 366 255 316 777.6 0 316 256 388 777.6 0 388 257 359 777.6 0 359 258 269 777.6 0 269 259 186 777.6 0 186 260 181 777.6 0 181 261 418 777.6 0 418 262 213 777.6 0 213 263 191 777.6 0 191 264 80 777.6 0 80 265 107 777.6 0 107 266 101 777.6 0 101 267 158 777.6 0 158 268 447 777.6 0 447 269 239 777.6 0 239 270 104 777.6 0 104 271 48 777.6 0 48 272 58 777.6 0 58 273 346 777.6 0 346 274 72 777.6 0 72 275 151 777.6 0 151 276 138 777.6 0 138 277 144 777.6 0 144 278 266 777.6 0 266 279 242 777.6 0 242 280 554 777.6 0 554 281 760 777.6 0 760 282 367 777.6 0 367 283 302 777.6 0 302 284 115 777.6 0 115 285 116 777.6 0 116 286 138 777.6 0 138 287 197 777.6 0 197 288 295 777.6 0 295 289 437 777.6 0 437 290 154 777.6 0 154 291 58 777.6 0 58 292 48 777.6 0 48 293 114 777.6 0 114 294 379 777.6 0 379 295 317 777.6 0 317 296 196 777.6 0 196 297 150 777.6 0 150 298 26 777.6 0 26 299 15 777.6 0 15 300 36 777.6 0 36 301 50 777.6 0 50 Finished in 20.59 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 390,351 Reads with adapters: 1,847 (0.5%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 1,831 (0.5%) Total basepairs processed: 84,580,494 bp Total written (filtered): 479,053 bp (0.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1847 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 19 6 0.0 3 0 5 1 20 1641 0.0 4 1626 12 0 2 1 21 2 0.0 4 0 2 44 18 0.0 4 0 0 0 0 18 54 1 0.0 4 0 0 0 0 1 55 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 89 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 0 2 105 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 2 0.0 4 0 0 0 0 2 127 148 0.0 4 0 0 0 0 148 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 153 3 0.0 4 0 0 0 0 3 154 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 243 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 1,831 Reads with adapters: 20 (1.1%) Reads written (passing filters): 1,831 (100.0%) Total basepairs processed: 479,053 bp Total written (filtered): 478,603 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 20 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.0% C: 0.0% G: 95.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 2 0.0 0 0 2 10 11 0.0 1 0 11 11 6 0.0 1 0 6 256 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.