This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.65 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 151,387 (4.8%) Reads that were too short: 59,551 (1.9%) Reads written (passing filters): 91,836 (2.9%) Total basepairs processed: 958,672,960 bp Total written (filtered): 21,174,339 bp (2.2%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 151387 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 838 777.6 0 838 7 48719 777.6 0 48719 8 146 777.6 0 146 9 7 777.6 0 7 10 6 777.6 0 6 11 13 777.6 0 13 12 11 777.6 0 11 13 338 777.6 0 338 14 123 777.6 0 123 15 109 777.6 0 109 16 28 777.6 0 28 17 2 777.6 0 2 18 1 777.6 0 1 19 2 777.6 0 2 20 2 777.6 0 2 22 2 777.6 0 2 23 2 777.6 0 2 24 7 777.6 0 7 25 22 777.6 0 22 26 22 777.6 0 22 27 24 777.6 0 24 28 1 777.6 0 1 29 4 777.6 0 4 30 5 777.6 0 5 31 5 777.6 0 5 32 4 777.6 0 4 33 8 777.6 0 8 34 8 777.6 0 8 35 4 777.6 0 4 36 2 777.6 0 2 37 1 777.6 0 1 38 28 777.6 0 28 39 4 777.6 0 4 41 3 777.6 0 3 42 3 777.6 0 3 43 4 777.6 0 4 44 4 777.6 0 4 45 1 777.6 0 1 46 3 777.6 0 3 47 2 777.6 0 2 48 3 777.6 0 3 49 9 777.6 0 9 50 7 777.6 0 7 51 3 777.6 0 3 52 4 777.6 0 4 53 7 777.6 0 7 54 8 777.6 0 8 55 4 777.6 0 4 56 6 777.6 0 6 57 7 777.6 0 7 58 5 777.6 0 5 59 6 777.6 0 6 60 9 777.6 0 9 61 16 777.6 0 16 62 23 777.6 0 23 63 4 777.6 0 4 64 30 777.6 0 30 65 4 777.6 0 4 66 8 777.6 0 8 67 1 777.6 0 1 68 1 777.6 0 1 69 4 777.6 0 4 70 2 777.6 0 2 71 4 777.6 0 4 72 5 777.6 0 5 73 10 777.6 0 10 74 22 777.6 0 22 75 37 777.6 0 37 76 148 777.6 0 148 77 27 777.6 0 27 78 91 777.6 0 91 79 137 777.6 0 137 80 425 777.6 0 425 81 141 777.6 0 141 82 336 777.6 0 336 83 155 777.6 0 155 84 281 777.6 0 281 85 214 777.6 0 214 86 105 777.6 0 105 87 119 777.6 0 119 88 516 777.6 0 516 89 1348 777.6 0 1348 90 152 777.6 0 152 91 97 777.6 0 97 92 338 777.6 0 338 93 300 777.6 0 300 94 124 777.6 0 124 95 129 777.6 0 129 96 186 777.6 0 186 97 80 777.6 0 80 98 307 777.6 0 307 99 632 777.6 0 632 100 127 777.6 0 127 101 32 777.6 0 32 102 389 777.6 0 389 103 100 777.6 0 100 104 204 777.6 0 204 105 458 777.6 0 458 106 225 777.6 0 225 107 53 777.6 0 53 108 79 777.6 0 79 109 219 777.6 0 219 110 229 777.6 0 229 111 246 777.6 0 246 112 308 777.6 0 308 113 162 777.6 0 162 114 268 777.6 0 268 115 123 777.6 0 123 116 86 777.6 0 86 117 121 777.6 0 121 118 398 777.6 0 398 119 197 777.6 0 197 120 317 777.6 0 317 121 351 777.6 0 351 122 255 777.6 0 255 123 239 777.6 0 239 124 214 777.6 0 214 125 191 777.6 0 191 126 202 777.6 0 202 127 212 777.6 0 212 128 156 777.6 0 156 129 295 777.6 0 295 130 296 777.6 0 296 131 179 777.6 0 179 132 239 777.6 0 239 133 319 777.6 0 319 134 266 777.6 0 266 135 259 777.6 0 259 136 207 777.6 0 207 137 249 777.6 0 249 138 295 777.6 0 295 139 514 777.6 0 514 140 694 777.6 0 694 141 311 777.6 0 311 142 259 777.6 0 259 143 229 777.6 0 229 144 372 777.6 0 372 145 260 777.6 0 260 146 369 777.6 0 369 147 391 777.6 0 391 148 337 777.6 0 337 149 221 777.6 0 221 150 132 777.6 0 132 151 211 777.6 0 211 152 343 777.6 0 343 153 332 777.6 0 332 154 233 777.6 0 233 155 255 777.6 0 255 156 328 777.6 0 328 157 347 777.6 0 347 158 294 777.6 0 294 159 392 777.6 0 392 160 917 777.6 0 917 161 700 777.6 0 700 162 750 777.6 0 750 163 325 777.6 0 325 164 292 777.6 0 292 165 269 777.6 0 269 166 197 777.6 0 197 167 225 777.6 0 225 168 309 777.6 0 309 169 404 777.6 0 404 170 189 777.6 0 189 171 187 777.6 0 187 172 350 777.6 0 350 173 494 777.6 0 494 174 156 777.6 0 156 175 169 777.6 0 169 176 203 777.6 0 203 177 317 777.6 0 317 178 334 777.6 0 334 179 368 777.6 0 368 180 399 777.6 0 399 181 358 777.6 0 358 182 520 777.6 0 520 183 453 777.6 0 453 184 438 777.6 0 438 185 355 777.6 0 355 186 341 777.6 0 341 187 2105 777.6 0 2105 188 1210 777.6 0 1210 189 1865 777.6 0 1865 190 511 777.6 0 511 191 392 777.6 0 392 192 198 777.6 0 198 193 233 777.6 0 233 194 183 777.6 0 183 195 159 777.6 0 159 196 347 777.6 0 347 197 692 777.6 0 692 198 306 777.6 0 306 199 186 777.6 0 186 200 232 777.6 0 232 201 454 777.6 0 454 202 765 777.6 0 765 203 616 777.6 0 616 204 404 777.6 0 404 205 409 777.6 0 409 206 405 777.6 0 405 207 311 777.6 0 311 208 366 777.6 0 366 209 866 777.6 0 866 210 381 777.6 0 381 211 451 777.6 0 451 212 506 777.6 0 506 213 321 777.6 0 321 214 306 777.6 0 306 215 284 777.6 0 284 216 391 777.6 0 391 217 380 777.6 0 380 218 354 777.6 0 354 219 334 777.6 0 334 220 382 777.6 0 382 221 439 777.6 0 439 222 663 777.6 0 663 223 528 777.6 0 528 224 282 777.6 0 282 225 276 777.6 0 276 226 303 777.6 0 303 227 351 777.6 0 351 228 276 777.6 0 276 229 571 777.6 0 571 230 742 777.6 0 742 231 567 777.6 0 567 232 647 777.6 0 647 233 611 777.6 0 611 234 502 777.6 0 502 235 261 777.6 0 261 236 215 777.6 0 215 237 360 777.6 0 360 238 386 777.6 0 386 239 1817 777.6 0 1817 240 973 777.6 0 973 241 551 777.6 0 551 242 475 777.6 0 475 243 406 777.6 0 406 244 394 777.6 0 394 245 410 777.6 0 410 246 394 777.6 0 394 247 207 777.6 0 207 248 185 777.6 0 185 249 211 777.6 0 211 250 338 777.6 0 338 251 520 777.6 0 520 252 402 777.6 0 402 253 435 777.6 0 435 254 406 777.6 0 406 255 465 777.6 0 465 256 276 777.6 0 276 257 214 777.6 0 214 258 218 777.6 0 218 259 264 777.6 0 264 260 311 777.6 0 311 261 286 777.6 0 286 262 277 777.6 0 277 263 332 777.6 0 332 264 366 777.6 0 366 265 611 777.6 0 611 266 436 777.6 0 436 267 270 777.6 0 270 268 235 777.6 0 235 269 293 777.6 0 293 270 487 777.6 0 487 271 420 777.6 0 420 272 451 777.6 0 451 273 486 777.6 0 486 274 391 777.6 0 391 275 1193 777.6 0 1193 276 1064 777.6 0 1064 277 1854 777.6 0 1854 278 726 777.6 0 726 279 678 777.6 0 678 280 1189 777.6 0 1189 281 927 777.6 0 927 282 1238 777.6 0 1238 283 911 777.6 0 911 284 1492 777.6 0 1492 285 984 777.6 0 984 286 530 777.6 0 530 287 559 777.6 0 559 288 770 777.6 0 770 289 2072 777.6 0 2072 290 445 777.6 0 445 291 470 777.6 0 470 292 664 777.6 0 664 293 538 777.6 0 538 294 495 777.6 0 495 295 756 777.6 0 756 296 655 777.6 0 655 297 524 777.6 0 524 298 1158 777.6 0 1158 299 896 777.6 0 896 300 3407 777.6 0 3407 301 1931 777.6 0 1931 Finished in 4.08 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 91,836 Reads with adapters: 49,327 (53.7%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 49,304 (53.7%) Total basepairs processed: 21,174,339 bp Total written (filtered): 13,655,185 bp (64.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49327 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 14 0.0 3 0 0 12 2 16 518 0.0 3 0 468 29 21 17 48437 0.0 3 46868 1353 180 36 18 136 0.0 3 12 110 7 7 19 122 0.0 3 0 0 82 40 20 54 0.0 3 3 0 0 51 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 1 27 1 0.0 3 1 29 1 0.0 3 1 30 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.27 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 49,304 Reads with adapters: 5,194 (10.5%) Reads written (passing filters): 49,304 (100.0%) Total basepairs processed: 13,655,185 bp Total written (filtered): 13,639,381 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5194 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.9% C: 63.0% G: 6.2% T: 8.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4989 770.4 0 4989 4 193 192.6 0 193 5 7 48.1 0 7 6 5 12.0 0 5