This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.16 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 96,849 (3.0%) Reads that were too short: 37,821 (1.2%) Reads written (passing filters): 59,028 (1.9%) Total basepairs processed: 958,672,960 bp Total written (filtered): 10,110,935 bp (1.1%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 96849 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 67 777.6 0 67 7 60 777.6 0 60 8 136 777.6 0 136 9 8120 777.6 0 8120 10 30 777.6 0 30 11 12 777.6 0 12 12 7 777.6 0 7 13 17 777.6 0 17 14 13 777.6 0 13 15 12 777.6 0 12 16 13 777.6 0 13 17 2 777.6 0 2 18 9 777.6 0 9 19 12 777.6 0 12 20 17 777.6 0 17 21 14 777.6 0 14 22 10 777.6 0 10 23 6 777.6 0 6 24 9 777.6 0 9 25 5 777.6 0 5 26 14 777.6 0 14 27 9 777.6 0 9 28 24 777.6 0 24 29 11 777.6 0 11 30 10 777.6 0 10 31 8 777.6 0 8 32 8 777.6 0 8 33 12 777.6 0 12 34 12 777.6 0 12 35 18 777.6 0 18 36 23 777.6 0 23 37 13 777.6 0 13 38 54 777.6 0 54 39 145 777.6 0 145 40 35 777.6 0 35 41 9 777.6 0 9 42 57 777.6 0 57 43 11 777.6 0 11 44 4 777.6 0 4 45 35 777.6 0 35 46 4 777.6 0 4 47 7 777.6 0 7 48 10 777.6 0 10 49 15 777.6 0 15 50 8 777.6 0 8 51 16 777.6 0 16 52 15 777.6 0 15 53 73 777.6 0 73 54 112 777.6 0 112 55 45 777.6 0 45 56 16 777.6 0 16 57 35 777.6 0 35 58 14 777.6 0 14 59 12 777.6 0 12 60 46 777.6 0 46 61 12 777.6 0 12 62 10 777.6 0 10 63 8 777.6 0 8 64 19 777.6 0 19 65 9 777.6 0 9 66 208 777.6 0 208 67 18 777.6 0 18 68 13 777.6 0 13 69 8 777.6 0 8 70 11 777.6 0 11 71 11 777.6 0 11 72 15 777.6 0 15 73 10 777.6 0 10 74 15 777.6 0 15 75 7 777.6 0 7 76 23 777.6 0 23 77 15 777.6 0 15 78 34 777.6 0 34 79 34 777.6 0 34 80 62 777.6 0 62 81 50 777.6 0 50 82 109 777.6 0 109 83 56 777.6 0 56 84 65 777.6 0 65 85 45 777.6 0 45 86 56 777.6 0 56 87 106 777.6 0 106 88 92 777.6 0 92 89 89 777.6 0 89 90 24 777.6 0 24 91 376 777.6 0 376 92 118 777.6 0 118 93 208 777.6 0 208 94 118 777.6 0 118 95 103 777.6 0 103 96 26 777.6 0 26 97 40 777.6 0 40 98 92 777.6 0 92 99 130 777.6 0 130 100 38 777.6 0 38 101 82 777.6 0 82 102 136 777.6 0 136 103 335 777.6 0 335 104 324 777.6 0 324 105 373 777.6 0 373 106 49 777.6 0 49 107 86 777.6 0 86 108 169 777.6 0 169 109 212 777.6 0 212 110 35 777.6 0 35 111 222 777.6 0 222 112 197 777.6 0 197 113 436 777.6 0 436 114 155 777.6 0 155 115 178 777.6 0 178 116 84 777.6 0 84 117 109 777.6 0 109 118 87 777.6 0 87 119 78 777.6 0 78 120 165 777.6 0 165 121 199 777.6 0 199 122 118 777.6 0 118 123 243 777.6 0 243 124 186 777.6 0 186 125 1373 777.6 0 1373 126 1108 777.6 0 1108 127 943 777.6 0 943 128 130 777.6 0 130 129 112 777.6 0 112 130 324 777.6 0 324 131 92 777.6 0 92 132 185 777.6 0 185 133 186 777.6 0 186 134 283 777.6 0 283 135 252 777.6 0 252 136 115 777.6 0 115 137 195 777.6 0 195 138 326 777.6 0 326 139 556 777.6 0 556 140 230 777.6 0 230 141 143 777.6 0 143 142 109 777.6 0 109 143 251 777.6 0 251 144 503 777.6 0 503 145 299 777.6 0 299 146 352 777.6 0 352 147 254 777.6 0 254 148 156 777.6 0 156 149 149 777.6 0 149 150 1940 777.6 0 1940 151 531 777.6 0 531 152 996 777.6 0 996 153 167 777.6 0 167 154 336 777.6 0 336 155 361 777.6 0 361 156 764 777.6 0 764 157 702 777.6 0 702 158 497 777.6 0 497 159 958 777.6 0 958 160 6751 777.6 0 6751 161 3174 777.6 0 3174 162 3832 777.6 0 3832 163 336 777.6 0 336 164 671 777.6 0 671 165 366 777.6 0 366 166 514 777.6 0 514 167 218 777.6 0 218 168 281 777.6 0 281 169 229 777.6 0 229 170 205 777.6 0 205 171 283 777.6 0 283 172 382 777.6 0 382 173 184 777.6 0 184 174 102 777.6 0 102 175 182 777.6 0 182 176 152 777.6 0 152 177 214 777.6 0 214 178 265 777.6 0 265 179 260 777.6 0 260 180 707 777.6 0 707 181 969 777.6 0 969 182 2964 777.6 0 2964 183 455 777.6 0 455 184 237 777.6 0 237 185 212 777.6 0 212 186 227 777.6 0 227 187 140 777.6 0 140 188 220 777.6 0 220 189 260 777.6 0 260 190 302 777.6 0 302 191 233 777.6 0 233 192 119 777.6 0 119 193 84 777.6 0 84 194 56 777.6 0 56 195 83 777.6 0 83 196 188 777.6 0 188 197 212 777.6 0 212 198 97 777.6 0 97 199 80 777.6 0 80 200 95 777.6 0 95 201 357 777.6 0 357 202 248 777.6 0 248 203 116 777.6 0 116 204 124 777.6 0 124 205 163 777.6 0 163 206 144 777.6 0 144 207 255 777.6 0 255 208 313 777.6 0 313 209 341 777.6 0 341 210 167 777.6 0 167 211 407 777.6 0 407 212 1983 777.6 0 1983 213 1239 777.6 0 1239 214 1254 777.6 0 1254 215 244 777.6 0 244 216 411 777.6 0 411 217 430 777.6 0 430 218 342 777.6 0 342 219 631 777.6 0 631 220 203 777.6 0 203 221 387 777.6 0 387 222 367 777.6 0 367 223 235 777.6 0 235 224 243 777.6 0 243 225 813 777.6 0 813 226 345 777.6 0 345 227 327 777.6 0 327 228 287 777.6 0 287 229 236 777.6 0 236 230 143 777.6 0 143 231 125 777.6 0 125 232 187 777.6 0 187 233 239 777.6 0 239 234 134 777.6 0 134 235 96 777.6 0 96 236 142 777.6 0 142 237 238 777.6 0 238 238 163 777.6 0 163 239 526 777.6 0 526 240 234 777.6 0 234 241 343 777.6 0 343 242 371 777.6 0 371 243 398 777.6 0 398 244 284 777.6 0 284 245 410 777.6 0 410 246 584 777.6 0 584 247 366 777.6 0 366 248 209 777.6 0 209 249 177 777.6 0 177 250 254 777.6 0 254 251 447 777.6 0 447 252 350 777.6 0 350 253 368 777.6 0 368 254 640 777.6 0 640 255 1870 777.6 0 1870 256 1523 777.6 0 1523 257 844 777.6 0 844 258 280 777.6 0 280 259 161 777.6 0 161 260 136 777.6 0 136 261 106 777.6 0 106 262 147 777.6 0 147 263 204 777.6 0 204 264 273 777.6 0 273 265 285 777.6 0 285 266 680 777.6 0 680 267 691 777.6 0 691 268 408 777.6 0 408 269 535 777.6 0 535 270 381 777.6 0 381 271 780 777.6 0 780 272 384 777.6 0 384 273 517 777.6 0 517 274 301 777.6 0 301 275 357 777.6 0 357 276 451 777.6 0 451 277 361 777.6 0 361 278 335 777.6 0 335 279 201 777.6 0 201 280 337 777.6 0 337 281 284 777.6 0 284 282 647 777.6 0 647 283 384 777.6 0 384 284 401 777.6 0 401 285 160 777.6 0 160 286 276 777.6 0 276 287 611 777.6 0 611 288 459 777.6 0 459 289 242 777.6 0 242 290 250 777.6 0 250 291 308 777.6 0 308 292 206 777.6 0 206 293 145 777.6 0 145 294 124 777.6 0 124 295 142 777.6 0 142 296 182 777.6 0 182 297 130 777.6 0 130 298 268 777.6 0 268 299 194 777.6 0 194 300 125 777.6 0 125 301 52 777.6 0 52 Finished in 2.37 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 59,028 Reads with adapters: 8,343 (14.1%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 8,316 (14.1%) Total basepairs processed: 10,110,935 bp Total written (filtered): 2,284,534 bp (22.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8343 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 53 0.0 3 0 49 1 3 17 8202 0.0 3 7899 258 34 11 18 23 0.0 3 2 19 2 19 11 0.0 3 2 0 6 3 20 4 0.0 3 0 0 0 4 28 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 5 0.0 3 0 1 0 4 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 54 2 0.0 3 1 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 74 2 0.0 3 1 0 0 1 76 1 0.0 3 0 0 1 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 1 88 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 8,316 Reads with adapters: 228 (2.7%) Reads written (passing filters): 8,316 (100.0%) Total basepairs processed: 2,284,534 bp Total written (filtered): 2,283,793 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 228 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.2% C: 12.7% G: 23.7% T: 11.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 172 129.9 0 172 4 55 32.5 0 55 5 1 8.1 0 1