This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 105.72 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 417,436 (13.1%) Reads that were too short: 5,039 (0.2%) Reads written (passing filters): 412,397 (12.9%) Total basepairs processed: 958,672,960 bp Total written (filtered): 77,981,983 bp (8.1%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 417436 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 115 777.6 0 115 7 203 777.6 0 203 8 4189 777.6 0 4189 9 8398 777.6 0 8398 10 7 777.6 0 7 11 18 777.6 0 18 12 19 777.6 0 19 13 17 777.6 0 17 14 16 777.6 0 16 15 19 777.6 0 19 16 11 777.6 0 11 17 25 777.6 0 25 18 26 777.6 0 26 19 22 777.6 0 22 20 13 777.6 0 13 21 14 777.6 0 14 22 14 777.6 0 14 23 15 777.6 0 15 24 22 777.6 0 22 25 74 777.6 0 74 26 63 777.6 0 63 27 65 777.6 0 65 28 19 777.6 0 19 29 16 777.6 0 16 30 25 777.6 0 25 31 17 777.6 0 17 32 31 777.6 0 31 33 19 777.6 0 19 34 23 777.6 0 23 35 54 777.6 0 54 36 132 777.6 0 132 37 3124 777.6 0 3124 38 31 777.6 0 31 39 13 777.6 0 13 40 14 777.6 0 14 41 17 777.6 0 17 42 20 777.6 0 20 43 16 777.6 0 16 44 21 777.6 0 21 45 24 777.6 0 24 46 10 777.6 0 10 47 14 777.6 0 14 48 9 777.6 0 9 49 11 777.6 0 11 50 20 777.6 0 20 51 12 777.6 0 12 52 9 777.6 0 9 53 23 777.6 0 23 54 13 777.6 0 13 55 21 777.6 0 21 56 84 777.6 0 84 57 31 777.6 0 31 58 66 777.6 0 66 59 7 777.6 0 7 60 57 777.6 0 57 61 26 777.6 0 26 62 21 777.6 0 21 63 17 777.6 0 17 64 14 777.6 0 14 65 25 777.6 0 25 66 16 777.6 0 16 67 23 777.6 0 23 68 33 777.6 0 33 69 50 777.6 0 50 70 486 777.6 0 486 71 289 777.6 0 289 72 893 777.6 0 893 73 12 777.6 0 12 74 15 777.6 0 15 75 15 777.6 0 15 76 62 777.6 0 62 77 46 777.6 0 46 78 37 777.6 0 37 79 44 777.6 0 44 80 52 777.6 0 52 81 54 777.6 0 54 82 293 777.6 0 293 83 430 777.6 0 430 84 269 777.6 0 269 85 80 777.6 0 80 86 600 777.6 0 600 87 35 777.6 0 35 88 14 777.6 0 14 89 22 777.6 0 22 90 27 777.6 0 27 91 29 777.6 0 29 92 47 777.6 0 47 93 141 777.6 0 141 94 130 777.6 0 130 95 217 777.6 0 217 96 628 777.6 0 628 97 314 777.6 0 314 98 417 777.6 0 417 99 422 777.6 0 422 100 221 777.6 0 221 101 417 777.6 0 417 102 287 777.6 0 287 103 339 777.6 0 339 104 102 777.6 0 102 105 156 777.6 0 156 106 225 777.6 0 225 107 351 777.6 0 351 108 786 777.6 0 786 109 1234 777.6 0 1234 110 2141 777.6 0 2141 111 3605 777.6 0 3605 112 7656 777.6 0 7656 113 151442 777.6 0 151442 114 93161 777.6 0 93161 115 77705 777.6 0 77705 116 422 777.6 0 422 117 84 777.6 0 84 118 128 777.6 0 128 119 84 777.6 0 84 120 98 777.6 0 98 121 221 777.6 0 221 122 376 777.6 0 376 123 101 777.6 0 101 124 129 777.6 0 129 125 200 777.6 0 200 126 141 777.6 0 141 127 173 777.6 0 173 128 208 777.6 0 208 129 219 777.6 0 219 130 299 777.6 0 299 131 173 777.6 0 173 132 253 777.6 0 253 133 610 777.6 0 610 134 6259 777.6 0 6259 135 20950 777.6 0 20950 136 4229 777.6 0 4229 137 8523 777.6 0 8523 138 87 777.6 0 87 139 184 777.6 0 184 140 244 777.6 0 244 141 51 777.6 0 51 142 33 777.6 0 33 143 195 777.6 0 195 144 87 777.6 0 87 145 125 777.6 0 125 146 22 777.6 0 22 147 46 777.6 0 46 148 46 777.6 0 46 149 17 777.6 0 17 150 49 777.6 0 49 151 8 777.6 0 8 152 19 777.6 0 19 153 13 777.6 0 13 154 22 777.6 0 22 155 22 777.6 0 22 156 35 777.6 0 35 157 32 777.6 0 32 158 148 777.6 0 148 159 98 777.6 0 98 160 110 777.6 0 110 161 25 777.6 0 25 162 28 777.6 0 28 163 26 777.6 0 26 164 46 777.6 0 46 165 29 777.6 0 29 166 36 777.6 0 36 167 33 777.6 0 33 168 53 777.6 0 53 169 26 777.6 0 26 170 22 777.6 0 22 171 17 777.6 0 17 172 15 777.6 0 15 173 35 777.6 0 35 174 7 777.6 0 7 175 18 777.6 0 18 176 7 777.6 0 7 177 76 777.6 0 76 178 68 777.6 0 68 179 1229 777.6 0 1229 180 855 777.6 0 855 181 32 777.6 0 32 182 232 777.6 0 232 183 16 777.6 0 16 184 36 777.6 0 36 185 22 777.6 0 22 186 16 777.6 0 16 187 6 777.6 0 6 188 16 777.6 0 16 189 17 777.6 0 17 190 35 777.6 0 35 191 34 777.6 0 34 192 18 777.6 0 18 193 14 777.6 0 14 194 14 777.6 0 14 195 17 777.6 0 17 196 19 777.6 0 19 197 29 777.6 0 29 198 8 777.6 0 8 199 12 777.6 0 12 200 20 777.6 0 20 201 19 777.6 0 19 202 52 777.6 0 52 203 63 777.6 0 63 204 41 777.6 0 41 205 56 777.6 0 56 206 88 777.6 0 88 207 27 777.6 0 27 208 14 777.6 0 14 209 33 777.6 0 33 210 27 777.6 0 27 211 56 777.6 0 56 212 45 777.6 0 45 213 32 777.6 0 32 214 41 777.6 0 41 215 60 777.6 0 60 216 57 777.6 0 57 217 58 777.6 0 58 218 24 777.6 0 24 219 21 777.6 0 21 220 21 777.6 0 21 221 48 777.6 0 48 222 22 777.6 0 22 223 88 777.6 0 88 224 73 777.6 0 73 225 135 777.6 0 135 226 118 777.6 0 118 227 51 777.6 0 51 228 30 777.6 0 30 229 34 777.6 0 34 230 17 777.6 0 17 231 15 777.6 0 15 232 17 777.6 0 17 233 27 777.6 0 27 234 67 777.6 0 67 235 51 777.6 0 51 236 37 777.6 0 37 237 20 777.6 0 20 238 13 777.6 0 13 239 17 777.6 0 17 240 17 777.6 0 17 241 26 777.6 0 26 242 26 777.6 0 26 243 35 777.6 0 35 244 45 777.6 0 45 245 63 777.6 0 63 246 27 777.6 0 27 247 17 777.6 0 17 248 7 777.6 0 7 249 6 777.6 0 6 250 25 777.6 0 25 251 31 777.6 0 31 252 38 777.6 0 38 253 46 777.6 0 46 254 76 777.6 0 76 255 70 777.6 0 70 256 35 777.6 0 35 257 33 777.6 0 33 258 24 777.6 0 24 259 28 777.6 0 28 260 29 777.6 0 29 261 29 777.6 0 29 262 40 777.6 0 40 263 59 777.6 0 59 264 39 777.6 0 39 265 70 777.6 0 70 266 45 777.6 0 45 267 19 777.6 0 19 268 19 777.6 0 19 269 13 777.6 0 13 270 63 777.6 0 63 271 227 777.6 0 227 272 120 777.6 0 120 273 758 777.6 0 758 274 38 777.6 0 38 275 57 777.6 0 57 276 19 777.6 0 19 277 29 777.6 0 29 278 51 777.6 0 51 279 40 777.6 0 40 280 49 777.6 0 49 281 95 777.6 0 95 282 147 777.6 0 147 283 79 777.6 0 79 284 40 777.6 0 40 285 25 777.6 0 25 286 23 777.6 0 23 287 11 777.6 0 11 288 12 777.6 0 12 289 10 777.6 0 10 290 23 777.6 0 23 291 45 777.6 0 45 292 36 777.6 0 36 293 18 777.6 0 18 294 11 777.6 0 11 295 21 777.6 0 21 296 26 777.6 0 26 297 76 777.6 0 76 298 66 777.6 0 66 299 39 777.6 0 39 300 47 777.6 0 47 301 5 777.6 0 5 Finished in 19.80 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 412,397 Reads with adapters: 8,728 (2.1%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 8,723 (2.1%) Total basepairs processed: 77,981,983 bp Total written (filtered): 2,372,506 bp (3.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8728 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 1 19 34 0.0 3 0 28 2 0 4 20 8650 0.0 4 8526 120 4 21 27 0.0 4 10 17 22 1 0.0 4 0 0 1 23 3 0.0 4 0 0 0 0 3 29 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 8,723 Reads with adapters: 137 (1.6%) Reads written (passing filters): 8,723 (100.0%) Total basepairs processed: 2,372,506 bp Total written (filtered): 2,360,400 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 137 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 0.7% G: 65.0% T: 32.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 136.3 0 2 4 1 34.1 0 1 6 2 2.1 0 2 9 19 0.0 0 0 19 10 42 0.0 1 1 41 11 29 0.0 1 0 29 266 40 0.0 1 39 1 267 2 0.0 1 2