This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 109.37 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 565,613 (17.8%) Reads that were too short: 6,409 (0.2%) Reads written (passing filters): 559,204 (17.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 138,620,179 bp (14.5%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 565613 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 925 777.6 0 925 7 13543 777.6 0 13543 8 1909 777.6 0 1909 9 9213 777.6 0 9213 10 53 777.6 0 53 11 54 777.6 0 54 12 247 777.6 0 247 13 22 777.6 0 22 14 16 777.6 0 16 15 32 777.6 0 32 16 22 777.6 0 22 17 70 777.6 0 70 18 38 777.6 0 38 19 96 777.6 0 96 20 58 777.6 0 58 21 115 777.6 0 115 22 275 777.6 0 275 23 67 777.6 0 67 24 147 777.6 0 147 25 134 777.6 0 134 26 111 777.6 0 111 27 256 777.6 0 256 28 144 777.6 0 144 29 267 777.6 0 267 30 329 777.6 0 329 31 216 777.6 0 216 32 262 777.6 0 262 33 401 777.6 0 401 34 622 777.6 0 622 35 1095 777.6 0 1095 36 1132 777.6 0 1132 37 1759 777.6 0 1759 38 2008 777.6 0 2008 39 51969 777.6 0 51969 40 42382 777.6 0 42382 41 38887 777.6 0 38887 42 653 777.6 0 653 43 837 777.6 0 837 44 1189 777.6 0 1189 45 2040 777.6 0 2040 46 4992 777.6 0 4992 47 800 777.6 0 800 48 1050 777.6 0 1050 49 2492 777.6 0 2492 50 1284 777.6 0 1284 51 968 777.6 0 968 52 2132 777.6 0 2132 53 1519 777.6 0 1519 54 3247 777.6 0 3247 55 1809 777.6 0 1809 56 2931 777.6 0 2931 57 3173 777.6 0 3173 58 4907 777.6 0 4907 59 72991 777.6 0 72991 60 144427 777.6 0 144427 61 127915 777.6 0 127915 62 670 777.6 0 670 63 25 777.6 0 25 64 19 777.6 0 19 65 8 777.6 0 8 66 2 777.6 0 2 67 4 777.6 0 4 68 2 777.6 0 2 69 3 777.6 0 3 70 7 777.6 0 7 71 10 777.6 0 10 72 88 777.6 0 88 73 402 777.6 0 402 74 126 777.6 0 126 75 69 777.6 0 69 76 4 777.6 0 4 77 4 777.6 0 4 78 10 777.6 0 10 79 20 777.6 0 20 80 8 777.6 0 8 81 29 777.6 0 29 82 46 777.6 0 46 83 51 777.6 0 51 84 31 777.6 0 31 85 10 777.6 0 10 86 15 777.6 0 15 87 12 777.6 0 12 88 33 777.6 0 33 89 14 777.6 0 14 90 19 777.6 0 19 91 31 777.6 0 31 92 54 777.6 0 54 93 10 777.6 0 10 94 8 777.6 0 8 95 33 777.6 0 33 96 10 777.6 0 10 97 24 777.6 0 24 98 63 777.6 0 63 99 96 777.6 0 96 100 18 777.6 0 18 101 122 777.6 0 122 102 200 777.6 0 200 103 241 777.6 0 241 104 22 777.6 0 22 105 24 777.6 0 24 106 14 777.6 0 14 107 5 777.6 0 5 108 12 777.6 0 12 109 16 777.6 0 16 110 14 777.6 0 14 111 138 777.6 0 138 112 86 777.6 0 86 113 418 777.6 0 418 114 11 777.6 0 11 115 11 777.6 0 11 116 11 777.6 0 11 117 162 777.6 0 162 118 37 777.6 0 37 119 47 777.6 0 47 120 44 777.6 0 44 121 23 777.6 0 23 122 28 777.6 0 28 123 18 777.6 0 18 124 35 777.6 0 35 125 255 777.6 0 255 126 71 777.6 0 71 127 78 777.6 0 78 128 49 777.6 0 49 129 49 777.6 0 49 130 67 777.6 0 67 131 31 777.6 0 31 132 47 777.6 0 47 133 35 777.6 0 35 134 31 777.6 0 31 135 26 777.6 0 26 136 39 777.6 0 39 137 36 777.6 0 36 138 34 777.6 0 34 139 29 777.6 0 29 140 11 777.6 0 11 141 16 777.6 0 16 142 24 777.6 0 24 143 67 777.6 0 67 144 12 777.6 0 12 145 14 777.6 0 14 146 21 777.6 0 21 147 21 777.6 0 21 148 34 777.6 0 34 149 15 777.6 0 15 150 16 777.6 0 16 151 20 777.6 0 20 152 23 777.6 0 23 153 20 777.6 0 20 154 12 777.6 0 12 155 31 777.6 0 31 156 61 777.6 0 61 157 94 777.6 0 94 158 94 777.6 0 94 159 204 777.6 0 204 160 78 777.6 0 78 161 128 777.6 0 128 162 105 777.6 0 105 163 69 777.6 0 69 164 83 777.6 0 83 165 26 777.6 0 26 166 23 777.6 0 23 167 24 777.6 0 24 168 58 777.6 0 58 169 210 777.6 0 210 170 108 777.6 0 108 171 66 777.6 0 66 172 50 777.6 0 50 173 13 777.6 0 13 174 5 777.6 0 5 175 11 777.6 0 11 176 42 777.6 0 42 177 43 777.6 0 43 178 59 777.6 0 59 179 48 777.6 0 48 180 14 777.6 0 14 181 45 777.6 0 45 182 27 777.6 0 27 183 143 777.6 0 143 184 67 777.6 0 67 185 61 777.6 0 61 186 29 777.6 0 29 187 38 777.6 0 38 188 110 777.6 0 110 189 65 777.6 0 65 190 34 777.6 0 34 191 17 777.6 0 17 192 23 777.6 0 23 193 41 777.6 0 41 194 43 777.6 0 43 195 50 777.6 0 50 196 74 777.6 0 74 197 112 777.6 0 112 198 314 777.6 0 314 199 246 777.6 0 246 200 608 777.6 0 608 201 48 777.6 0 48 202 40 777.6 0 40 203 39 777.6 0 39 204 65 777.6 0 65 205 165 777.6 0 165 206 32 777.6 0 32 207 31 777.6 0 31 208 49 777.6 0 49 209 44 777.6 0 44 210 14 777.6 0 14 211 17 777.6 0 17 212 30 777.6 0 30 213 48 777.6 0 48 214 54 777.6 0 54 215 57 777.6 0 57 216 55 777.6 0 55 217 54 777.6 0 54 218 34 777.6 0 34 219 31 777.6 0 31 220 34 777.6 0 34 221 51 777.6 0 51 222 86 777.6 0 86 223 105 777.6 0 105 224 130 777.6 0 130 225 190 777.6 0 190 226 623 777.6 0 623 227 157 777.6 0 157 228 860 777.6 0 860 229 247 777.6 0 247 230 103 777.6 0 103 231 9 777.6 0 9 232 23 777.6 0 23 233 23 777.6 0 23 234 24 777.6 0 24 235 11 777.6 0 11 236 17 777.6 0 17 237 23 777.6 0 23 238 17 777.6 0 17 239 19 777.6 0 19 240 14 777.6 0 14 241 22 777.6 0 22 242 38 777.6 0 38 243 45 777.6 0 45 244 25 777.6 0 25 245 21 777.6 0 21 246 21 777.6 0 21 247 21 777.6 0 21 248 37 777.6 0 37 249 26 777.6 0 26 250 51 777.6 0 51 251 46 777.6 0 46 252 65 777.6 0 65 253 49 777.6 0 49 254 78 777.6 0 78 255 52 777.6 0 52 256 51 777.6 0 51 257 38 777.6 0 38 258 29 777.6 0 29 259 26 777.6 0 26 260 23 777.6 0 23 261 28 777.6 0 28 262 15 777.6 0 15 263 50 777.6 0 50 264 41 777.6 0 41 265 26 777.6 0 26 266 19 777.6 0 19 267 18 777.6 0 18 268 10 777.6 0 10 269 26 777.6 0 26 270 42 777.6 0 42 271 33 777.6 0 33 272 36 777.6 0 36 273 63 777.6 0 63 274 74 777.6 0 74 275 126 777.6 0 126 276 75 777.6 0 75 277 34 777.6 0 34 278 32 777.6 0 32 279 31 777.6 0 31 280 26 777.6 0 26 281 45 777.6 0 45 282 44 777.6 0 44 283 38 777.6 0 38 284 41 777.6 0 41 285 22 777.6 0 22 286 9 777.6 0 9 287 15 777.6 0 15 288 18 777.6 0 18 289 23 777.6 0 23 290 108 777.6 0 108 291 191 777.6 0 191 292 74 777.6 0 74 293 49 777.6 0 49 294 40 777.6 0 40 295 92 777.6 0 92 296 82 777.6 0 82 297 50 777.6 0 50 298 17 777.6 0 17 299 155 777.6 0 155 300 71 777.6 0 71 301 31 777.6 0 31 Finished in 27.13 s (49 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 559,204 Reads with adapters: 15,314 (2.7%) Reads that were too short: 517 (0.1%) Reads written (passing filters): 14,797 (2.6%) Total basepairs processed: 138,620,179 bp Total written (filtered): 4,002,102 bp (2.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15314 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 77 0.0 3 0 67 6 4 17 14062 0.0 3 13557 420 68 17 18 33 0.0 3 3 26 2 2 19 26 0.0 3 1 0 12 13 20 8 0.0 3 0 0 0 8 24 1 0.0 3 0 0 0 1 41 9 0.0 3 0 0 0 9 48 11 0.0 3 7 3 1 50 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 1 0.0 3 1 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 104 1 0.0 3 1 107 4 0.0 3 0 0 0 4 108 3 0.0 3 0 0 1 2 110 2 0.0 3 0 0 0 2 112 15 0.0 3 0 0 0 15 113 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 4 0.0 3 0 0 0 4 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 9 0.0 3 0 0 0 9 132 184 0.0 3 0 0 0 184 133 17 0.0 3 0 0 0 17 134 6 0.0 3 0 0 0 6 135 119 0.0 3 0 0 2 117 136 10 0.0 3 0 0 0 10 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 9 0.0 3 0 0 0 9 159 2 0.0 3 0 0 0 2 160 136 0.0 3 0 0 0 136 161 1 0.0 3 0 0 0 1 163 7 0.0 3 0 0 0 7 164 1 0.0 3 0 0 1 165 2 0.0 3 0 0 0 2 167 5 0.0 3 0 0 0 5 174 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 8 0.0 3 0 0 0 8 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 6 0.0 3 0 0 0 6 192 17 0.0 3 0 0 0 17 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 199 3 0.0 3 0 0 0 3 200 2 0.0 3 0 0 0 2 206 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 1 209 2 0.0 3 0 0 0 2 210 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 233 8 0.0 3 0 0 0 8 234 316 0.0 3 0 0 8 308 235 14 0.0 3 0 0 0 14 243 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 247 4 0.0 3 0 0 0 4 249 18 0.0 3 0 0 1 17 250 54 0.0 3 0 0 2 52 253 2 0.0 3 0 0 0 2 256 5 0.0 3 0 0 0 5 258 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 274 2 0.0 3 0 0 0 2 275 11 0.0 3 0 0 0 11 276 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 2 0.0 3 0 0 0 2 285 7 0.0 3 0 0 0 7 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 14,797 Reads with adapters: 1,111 (7.5%) Reads written (passing filters): 14,797 (100.0%) Total basepairs processed: 4,002,102 bp Total written (filtered): 3,998,716 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1111 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.8% C: 51.1% G: 8.1% T: 13.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1059 231.2 0 1059 4 51 57.8 0 51 5 1 14.5 0 1