This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 101.96 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 77,418 (2.4%) Reads that were too short: 1,871 (0.1%) Reads written (passing filters): 75,547 (2.4%) Total basepairs processed: 958,672,960 bp Total written (filtered): 19,639,047 bp (2.0%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 77418 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 598 777.6 0 598 7 13802 777.6 0 13802 8 14897 777.6 0 14897 9 27 777.6 0 27 10 4 777.6 0 4 11 46 777.6 0 46 12 60 777.6 0 60 13 4 777.6 0 4 14 7 777.6 0 7 15 8 777.6 0 8 16 4 777.6 0 4 17 5 777.6 0 5 18 16 777.6 0 16 19 10 777.6 0 10 20 5 777.6 0 5 21 18 777.6 0 18 22 9 777.6 0 9 23 5 777.6 0 5 24 10 777.6 0 10 25 15 777.6 0 15 26 12 777.6 0 12 27 18 777.6 0 18 28 71 777.6 0 71 29 14 777.6 0 14 30 2 777.6 0 2 31 3 777.6 0 3 32 8 777.6 0 8 33 7 777.6 0 7 34 6 777.6 0 6 35 13 777.6 0 13 36 11 777.6 0 11 37 40 777.6 0 40 38 83 777.6 0 83 39 25 777.6 0 25 40 174 777.6 0 174 41 25 777.6 0 25 42 31 777.6 0 31 43 240 777.6 0 240 44 48 777.6 0 48 45 35 777.6 0 35 46 20 777.6 0 20 47 68 777.6 0 68 48 46 777.6 0 46 49 105 777.6 0 105 50 110 777.6 0 110 51 130 777.6 0 130 52 221 777.6 0 221 53 486 777.6 0 486 54 938 777.6 0 938 55 18054 777.6 0 18054 56 5957 777.6 0 5957 57 11626 777.6 0 11626 58 56 777.6 0 56 59 10 777.6 0 10 60 2 777.6 0 2 61 3 777.6 0 3 62 3 777.6 0 3 63 38 777.6 0 38 64 2 777.6 0 2 65 3 777.6 0 3 66 4 777.6 0 4 67 3 777.6 0 3 68 19 777.6 0 19 69 6 777.6 0 6 70 9 777.6 0 9 71 6 777.6 0 6 72 12 777.6 0 12 73 12 777.6 0 12 74 6 777.6 0 6 75 10 777.6 0 10 76 4 777.6 0 4 77 6 777.6 0 6 78 24 777.6 0 24 79 11 777.6 0 11 80 16 777.6 0 16 81 27 777.6 0 27 82 48 777.6 0 48 83 71 777.6 0 71 84 149 777.6 0 149 85 2703 777.6 0 2703 86 1131 777.6 0 1131 87 1272 777.6 0 1272 88 11 777.6 0 11 89 3 777.6 0 3 90 2 777.6 0 2 91 4 777.6 0 4 92 3 777.6 0 3 93 23 777.6 0 23 94 2 777.6 0 2 95 2 777.6 0 2 96 3 777.6 0 3 97 1 777.6 0 1 98 3 777.6 0 3 99 5 777.6 0 5 100 5 777.6 0 5 101 4 777.6 0 4 102 34 777.6 0 34 103 15 777.6 0 15 104 18 777.6 0 18 105 2 777.6 0 2 106 5 777.6 0 5 107 2 777.6 0 2 108 6 777.6 0 6 109 2 777.6 0 2 110 5 777.6 0 5 111 1 777.6 0 1 112 1 777.6 0 1 113 2 777.6 0 2 114 5 777.6 0 5 115 1 777.6 0 1 116 4 777.6 0 4 117 4 777.6 0 4 118 13 777.6 0 13 119 7 777.6 0 7 120 14 777.6 0 14 121 2 777.6 0 2 122 6 777.6 0 6 123 4 777.6 0 4 124 6 777.6 0 6 125 3 777.6 0 3 126 7 777.6 0 7 127 6 777.6 0 6 128 10 777.6 0 10 129 31 777.6 0 31 130 7 777.6 0 7 131 6 777.6 0 6 132 13 777.6 0 13 133 28 777.6 0 28 134 13 777.6 0 13 135 5 777.6 0 5 136 12 777.6 0 12 137 26 777.6 0 26 138 125 777.6 0 125 139 79 777.6 0 79 140 56 777.6 0 56 141 3 777.6 0 3 142 5 777.6 0 5 143 5 777.6 0 5 144 17 777.6 0 17 145 2 777.6 0 2 146 4 777.6 0 4 147 16 777.6 0 16 148 33 777.6 0 33 149 70 777.6 0 70 150 14 777.6 0 14 151 38 777.6 0 38 152 3 777.6 0 3 153 5 777.6 0 5 154 3 777.6 0 3 155 3 777.6 0 3 156 7 777.6 0 7 157 43 777.6 0 43 158 46 777.6 0 46 159 45 777.6 0 45 160 4 777.6 0 4 161 7 777.6 0 7 162 9 777.6 0 9 163 20 777.6 0 20 164 33 777.6 0 33 165 8 777.6 0 8 166 9 777.6 0 9 167 16 777.6 0 16 168 20 777.6 0 20 169 22 777.6 0 22 170 5 777.6 0 5 171 9 777.6 0 9 172 6 777.6 0 6 173 3 777.6 0 3 174 7 777.6 0 7 175 6 777.6 0 6 176 4 777.6 0 4 177 6 777.6 0 6 178 8 777.6 0 8 179 3 777.6 0 3 180 8 777.6 0 8 181 10 777.6 0 10 182 7 777.6 0 7 183 20 777.6 0 20 184 20 777.6 0 20 185 13 777.6 0 13 186 11 777.6 0 11 187 45 777.6 0 45 188 42 777.6 0 42 189 78 777.6 0 78 190 52 777.6 0 52 191 13 777.6 0 13 192 131 777.6 0 131 193 1 777.6 0 1 194 3 777.6 0 3 195 2 777.6 0 2 196 1 777.6 0 1 198 2 777.6 0 2 199 5 777.6 0 5 200 13 777.6 0 13 201 3 777.6 0 3 202 1 777.6 0 1 203 1 777.6 0 1 204 2 777.6 0 2 205 4 777.6 0 4 206 13 777.6 0 13 207 14 777.6 0 14 208 3 777.6 0 3 209 3 777.6 0 3 210 4 777.6 0 4 211 5 777.6 0 5 212 4 777.6 0 4 213 6 777.6 0 6 214 8 777.6 0 8 215 18 777.6 0 18 216 7 777.6 0 7 217 10 777.6 0 10 218 25 777.6 0 25 219 225 777.6 0 225 220 22 777.6 0 22 221 12 777.6 0 12 222 4 777.6 0 4 223 5 777.6 0 5 224 9 777.6 0 9 225 11 777.6 0 11 226 2 777.6 0 2 227 6 777.6 0 6 228 3 777.6 0 3 229 3 777.6 0 3 230 11 777.6 0 11 231 7 777.6 0 7 232 1 777.6 0 1 233 5 777.6 0 5 234 6 777.6 0 6 235 6 777.6 0 6 236 4 777.6 0 4 237 1 777.6 0 1 238 4 777.6 0 4 239 6 777.6 0 6 240 6 777.6 0 6 241 4 777.6 0 4 242 1 777.6 0 1 243 3 777.6 0 3 244 3 777.6 0 3 245 6 777.6 0 6 246 10 777.6 0 10 247 5 777.6 0 5 248 2 777.6 0 2 249 3 777.6 0 3 250 4 777.6 0 4 251 24 777.6 0 24 252 6 777.6 0 6 253 11 777.6 0 11 254 15 777.6 0 15 255 11 777.6 0 11 256 3 777.6 0 3 257 4 777.6 0 4 258 12 777.6 0 12 259 48 777.6 0 48 260 2 777.6 0 2 261 1 777.6 0 1 262 6 777.6 0 6 263 7 777.6 0 7 264 8 777.6 0 8 265 11 777.6 0 11 266 9 777.6 0 9 267 7 777.6 0 7 268 11 777.6 0 11 269 3 777.6 0 3 270 1 777.6 0 1 271 8 777.6 0 8 272 5 777.6 0 5 273 5 777.6 0 5 274 15 777.6 0 15 275 21 777.6 0 21 276 170 777.6 0 170 277 103 777.6 0 103 278 188 777.6 0 188 279 24 777.6 0 24 280 13 777.6 0 13 281 50 777.6 0 50 282 47 777.6 0 47 283 19 777.6 0 19 284 12 777.6 0 12 285 21 777.6 0 21 286 9 777.6 0 9 287 16 777.6 0 16 288 14 777.6 0 14 289 16 777.6 0 16 290 6 777.6 0 6 291 9 777.6 0 9 292 8 777.6 0 8 293 8 777.6 0 8 294 10 777.6 0 10 295 10 777.6 0 10 296 56 777.6 0 56 297 11 777.6 0 11 298 16 777.6 0 16 299 78 777.6 0 78 300 169 777.6 0 169 301 6 777.6 0 6 Finished in 4.35 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 75,547 Reads with adapters: 15,714 (20.8%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 15,713 (20.8%) Total basepairs processed: 19,639,047 bp Total written (filtered): 4,286,665 bp (21.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15714 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 50 0.0 3 0 45 1 2 2 20 15510 0.0 4 15299 205 5 1 21 31 0.0 4 4 26 1 22 7 0.0 4 4 1 2 23 73 0.0 4 68 0 0 5 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 29 1 0.0 4 0 0 1 59 1 0.0 4 0 0 0 0 1 76 1 0.0 4 1 123 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 29 0.0 4 0 0 0 0 29 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 15,713 Reads with adapters: 238 (1.5%) Reads written (passing filters): 15,713 (100.0%) Total basepairs processed: 4,286,665 bp Total written (filtered): 4,283,565 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 238 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 1.7% G: 97.1% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 4 61.4 0 4 10 6 0.0 1 0 6 11 222 0.0 1 1 221 12 4 0.0 1 0 4 267 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.