This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.94 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 120,156 (3.8%) Reads that were too short: 38,165 (1.2%) Reads written (passing filters): 81,991 (2.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 21,696,762 bp (2.3%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 120156 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1423 777.6 0 1423 7 4328 777.6 0 4328 8 720 777.6 0 720 9 56446 777.6 0 56446 10 63 777.6 0 63 11 1323 777.6 0 1323 12 35 777.6 0 35 13 3 777.6 0 3 14 2 777.6 0 2 15 3 777.6 0 3 16 4 777.6 0 4 17 2 777.6 0 2 18 4 777.6 0 4 19 5 777.6 0 5 20 2 777.6 0 2 21 4 777.6 0 4 22 4 777.6 0 4 23 5 777.6 0 5 24 11 777.6 0 11 25 1 777.6 0 1 26 3 777.6 0 3 27 11 777.6 0 11 28 11 777.6 0 11 29 29 777.6 0 29 30 8 777.6 0 8 31 4 777.6 0 4 32 4 777.6 0 4 33 6 777.6 0 6 34 6 777.6 0 6 35 1 777.6 0 1 36 7 777.6 0 7 37 14 777.6 0 14 38 9 777.6 0 9 39 9 777.6 0 9 40 5 777.6 0 5 41 5 777.6 0 5 42 2 777.6 0 2 43 10 777.6 0 10 44 7 777.6 0 7 45 9 777.6 0 9 46 6 777.6 0 6 47 8 777.6 0 8 48 9 777.6 0 9 49 6 777.6 0 6 50 4 777.6 0 4 51 4 777.6 0 4 52 15 777.6 0 15 53 32 777.6 0 32 54 8 777.6 0 8 55 14 777.6 0 14 56 11 777.6 0 11 57 7 777.6 0 7 58 6 777.6 0 6 59 15 777.6 0 15 60 12 777.6 0 12 61 53 777.6 0 53 62 182 777.6 0 182 63 99 777.6 0 99 64 140 777.6 0 140 65 14 777.6 0 14 66 10 777.6 0 10 67 21 777.6 0 21 68 6 777.6 0 6 69 21 777.6 0 21 70 11 777.6 0 11 71 12 777.6 0 12 72 18 777.6 0 18 73 21 777.6 0 21 74 44 777.6 0 44 75 28 777.6 0 28 76 54 777.6 0 54 77 81 777.6 0 81 78 798 777.6 0 798 79 539 777.6 0 539 80 674 777.6 0 674 81 704 777.6 0 704 82 190 777.6 0 190 83 182 777.6 0 182 84 74 777.6 0 74 85 90 777.6 0 90 86 25 777.6 0 25 87 96 777.6 0 96 88 48 777.6 0 48 89 42 777.6 0 42 90 44 777.6 0 44 91 34 777.6 0 34 92 109 777.6 0 109 93 40 777.6 0 40 94 71 777.6 0 71 95 80 777.6 0 80 96 37 777.6 0 37 97 24 777.6 0 24 98 49 777.6 0 49 99 18 777.6 0 18 100 35 777.6 0 35 101 17 777.6 0 17 102 20 777.6 0 20 103 13 777.6 0 13 104 38 777.6 0 38 105 12 777.6 0 12 106 10 777.6 0 10 107 15 777.6 0 15 108 26 777.6 0 26 109 81 777.6 0 81 110 55 777.6 0 55 111 38 777.6 0 38 112 16 777.6 0 16 113 40 777.6 0 40 114 32 777.6 0 32 115 49 777.6 0 49 116 559 777.6 0 559 117 210 777.6 0 210 118 203 777.6 0 203 119 62 777.6 0 62 120 55 777.6 0 55 121 89 777.6 0 89 122 95 777.6 0 95 123 95 777.6 0 95 124 119 777.6 0 119 125 89 777.6 0 89 126 91 777.6 0 91 127 62 777.6 0 62 128 88 777.6 0 88 129 64 777.6 0 64 130 104 777.6 0 104 131 93 777.6 0 93 132 43 777.6 0 43 133 64 777.6 0 64 134 59 777.6 0 59 135 100 777.6 0 100 136 80 777.6 0 80 137 93 777.6 0 93 138 91 777.6 0 91 139 104 777.6 0 104 140 63 777.6 0 63 141 133 777.6 0 133 142 137 777.6 0 137 143 73 777.6 0 73 144 98 777.6 0 98 145 85 777.6 0 85 146 80 777.6 0 80 147 80 777.6 0 80 148 52 777.6 0 52 149 61 777.6 0 61 150 56 777.6 0 56 151 79 777.6 0 79 152 101 777.6 0 101 153 80 777.6 0 80 154 65 777.6 0 65 155 123 777.6 0 123 156 122 777.6 0 122 157 138 777.6 0 138 158 134 777.6 0 134 159 107 777.6 0 107 160 128 777.6 0 128 161 98 777.6 0 98 162 172 777.6 0 172 163 119 777.6 0 119 164 127 777.6 0 127 165 111 777.6 0 111 166 126 777.6 0 126 167 180 777.6 0 180 168 201 777.6 0 201 169 102 777.6 0 102 170 118 777.6 0 118 171 177 777.6 0 177 172 122 777.6 0 122 173 89 777.6 0 89 174 54 777.6 0 54 175 85 777.6 0 85 176 94 777.6 0 94 177 183 777.6 0 183 178 170 777.6 0 170 179 205 777.6 0 205 180 154 777.6 0 154 181 1031 777.6 0 1031 182 195 777.6 0 195 183 455 777.6 0 455 184 131 777.6 0 131 185 154 777.6 0 154 186 160 777.6 0 160 187 264 777.6 0 264 188 144 777.6 0 144 189 166 777.6 0 166 190 423 777.6 0 423 191 218 777.6 0 218 192 166 777.6 0 166 193 108 777.6 0 108 194 61 777.6 0 61 195 121 777.6 0 121 196 172 777.6 0 172 197 243 777.6 0 243 198 123 777.6 0 123 199 87 777.6 0 87 200 75 777.6 0 75 201 115 777.6 0 115 202 129 777.6 0 129 203 135 777.6 0 135 204 129 777.6 0 129 205 136 777.6 0 136 206 101 777.6 0 101 207 198 777.6 0 198 208 91 777.6 0 91 209 190 777.6 0 190 210 212 777.6 0 212 211 114 777.6 0 114 212 377 777.6 0 377 213 189 777.6 0 189 214 189 777.6 0 189 215 157 777.6 0 157 216 245 777.6 0 245 217 547 777.6 0 547 218 367 777.6 0 367 219 240 777.6 0 240 220 179 777.6 0 179 221 419 777.6 0 419 222 237 777.6 0 237 223 193 777.6 0 193 224 125 777.6 0 125 225 1021 777.6 0 1021 226 3679 777.6 0 3679 227 2889 777.6 0 2889 228 244 777.6 0 244 229 215 777.6 0 215 230 269 777.6 0 269 231 125 777.6 0 125 232 116 777.6 0 116 233 149 777.6 0 149 234 105 777.6 0 105 235 167 777.6 0 167 236 131 777.6 0 131 237 662 777.6 0 662 238 924 777.6 0 924 239 268 777.6 0 268 240 154 777.6 0 154 241 168 777.6 0 168 242 134 777.6 0 134 243 129 777.6 0 129 244 138 777.6 0 138 245 159 777.6 0 159 246 191 777.6 0 191 247 467 777.6 0 467 248 180 777.6 0 180 249 293 777.6 0 293 250 243 777.6 0 243 251 209 777.6 0 209 252 140 777.6 0 140 253 151 777.6 0 151 254 96 777.6 0 96 255 109 777.6 0 109 256 130 777.6 0 130 257 130 777.6 0 130 258 139 777.6 0 139 259 154 777.6 0 154 260 737 777.6 0 737 261 794 777.6 0 794 262 1315 777.6 0 1315 263 605 777.6 0 605 264 229 777.6 0 229 265 448 777.6 0 448 266 161 777.6 0 161 267 144 777.6 0 144 268 180 777.6 0 180 269 171 777.6 0 171 270 279 777.6 0 279 271 303 777.6 0 303 272 306 777.6 0 306 273 226 777.6 0 226 274 127 777.6 0 127 275 120 777.6 0 120 276 297 777.6 0 297 277 310 777.6 0 310 278 324 777.6 0 324 279 330 777.6 0 330 280 211 777.6 0 211 281 252 777.6 0 252 282 309 777.6 0 309 283 403 777.6 0 403 284 279 777.6 0 279 285 1137 777.6 0 1137 286 942 777.6 0 942 287 4092 777.6 0 4092 288 545 777.6 0 545 289 422 777.6 0 422 290 430 777.6 0 430 291 357 777.6 0 357 292 129 777.6 0 129 293 126 777.6 0 126 294 221 777.6 0 221 295 302 777.6 0 302 296 611 777.6 0 611 297 182 777.6 0 182 298 215 777.6 0 215 299 89 777.6 0 89 300 73 777.6 0 73 301 55 777.6 0 55 Finished in 3.95 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 81,991 Reads with adapters: 58,108 (70.9%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 58,075 (70.8%) Total basepairs processed: 21,696,762 bp Total written (filtered): 15,970,136 bp (73.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58108 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 5 0.0 3 0 0 3 2 16 271 0.0 3 0 244 20 7 17 57555 0.0 3 55425 1897 175 58 18 135 0.0 3 10 107 6 12 19 27 0.0 3 1 0 12 14 20 58 0.0 3 1 0 0 57 45 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 3 0.0 3 0 0 0 3 111 1 0.0 3 0 0 0 1 112 4 0.0 3 0 0 0 4 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 132 4 0.0 3 0 0 0 4 145 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 5 0.0 3 0 0 0 5 212 1 0.0 3 0 0 0 1 220 8 0.0 3 0 0 1 7 223 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 1 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.70 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 58,075 Reads with adapters: 5,850 (10.1%) Reads written (passing filters): 58,075 (100.0%) Total basepairs processed: 15,970,136 bp Total written (filtered): 15,952,169 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5850 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.5% C: 7.8% G: 33.6% T: 12.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5442 907.4 0 5442 4 405 226.9 0 405 5 1 56.7 0 1 6 1 14.2 0 1 10 1 0.1 1 0 1