This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 105.17 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 418,065 (13.1%) Reads that were too short: 46,629 (1.5%) Reads written (passing filters): 371,436 (11.7%) Total basepairs processed: 958,672,960 bp Total written (filtered): 81,075,758 bp (8.5%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 418065 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3077 777.6 0 3077 7 58712 777.6 0 58712 8 141 777.6 0 141 9 1896 777.6 0 1896 10 34 777.6 0 34 11 18 777.6 0 18 12 27 777.6 0 27 13 16 777.6 0 16 14 22 777.6 0 22 15 26 777.6 0 26 16 41 777.6 0 41 17 26 777.6 0 26 18 26 777.6 0 26 19 24 777.6 0 24 20 35 777.6 0 35 21 34 777.6 0 34 22 22 777.6 0 22 23 24 777.6 0 24 24 20 777.6 0 20 25 12 777.6 0 12 26 19 777.6 0 19 27 25 777.6 0 25 28 27 777.6 0 27 29 19 777.6 0 19 30 15 777.6 0 15 31 28 777.6 0 28 32 22 777.6 0 22 33 26 777.6 0 26 34 23 777.6 0 23 35 35 777.6 0 35 36 26 777.6 0 26 37 28 777.6 0 28 38 14 777.6 0 14 39 17 777.6 0 17 40 32 777.6 0 32 41 58 777.6 0 58 42 54 777.6 0 54 43 51 777.6 0 51 44 52 777.6 0 52 45 45 777.6 0 45 46 39 777.6 0 39 47 43 777.6 0 43 48 21 777.6 0 21 49 193 777.6 0 193 50 296 777.6 0 296 51 350 777.6 0 350 52 389 777.6 0 389 53 73 777.6 0 73 54 57 777.6 0 57 55 83 777.6 0 83 56 72 777.6 0 72 57 39 777.6 0 39 58 58 777.6 0 58 59 309 777.6 0 309 60 288 777.6 0 288 61 958 777.6 0 958 62 145 777.6 0 145 63 94 777.6 0 94 64 72 777.6 0 72 65 224 777.6 0 224 66 176 777.6 0 176 67 269 777.6 0 269 68 488 777.6 0 488 69 843 777.6 0 843 70 875 777.6 0 875 71 1694 777.6 0 1694 72 3157 777.6 0 3157 73 833 777.6 0 833 74 710 777.6 0 710 75 1934 777.6 0 1934 76 1187 777.6 0 1187 77 833 777.6 0 833 78 1719 777.6 0 1719 79 1115 777.6 0 1115 80 2178 777.6 0 2178 81 1052 777.6 0 1052 82 1875 777.6 0 1875 83 1915 777.6 0 1915 84 3246 777.6 0 3246 85 62963 777.6 0 62963 86 69758 777.6 0 69758 87 94808 777.6 0 94808 88 380 777.6 0 380 89 19 777.6 0 19 90 20 777.6 0 20 91 9 777.6 0 9 92 5 777.6 0 5 93 9 777.6 0 9 94 12 777.6 0 12 95 18 777.6 0 18 96 18 777.6 0 18 97 14 777.6 0 14 98 14 777.6 0 14 99 12 777.6 0 12 100 11 777.6 0 11 101 9 777.6 0 9 102 14 777.6 0 14 103 14 777.6 0 14 104 12 777.6 0 12 105 15 777.6 0 15 106 6 777.6 0 6 107 10 777.6 0 10 108 11 777.6 0 11 109 9 777.6 0 9 110 10 777.6 0 10 111 8 777.6 0 8 112 8 777.6 0 8 113 12 777.6 0 12 114 17 777.6 0 17 115 14 777.6 0 14 116 13 777.6 0 13 117 12 777.6 0 12 118 14 777.6 0 14 119 4 777.6 0 4 120 7 777.6 0 7 121 20 777.6 0 20 122 44 777.6 0 44 123 24 777.6 0 24 124 18 777.6 0 18 125 13 777.6 0 13 126 11 777.6 0 11 127 17 777.6 0 17 128 26 777.6 0 26 129 28 777.6 0 28 130 87 777.6 0 87 131 26 777.6 0 26 132 35 777.6 0 35 133 39 777.6 0 39 134 30 777.6 0 30 135 48 777.6 0 48 136 37 777.6 0 37 137 28 777.6 0 28 138 26 777.6 0 26 139 48 777.6 0 48 140 40 777.6 0 40 141 23 777.6 0 23 142 28 777.6 0 28 143 37 777.6 0 37 144 55 777.6 0 55 145 32 777.6 0 32 146 40 777.6 0 40 147 35 777.6 0 35 148 74 777.6 0 74 149 609 777.6 0 609 150 660 777.6 0 660 151 436 777.6 0 436 152 554 777.6 0 554 153 533 777.6 0 533 154 560 777.6 0 560 155 420 777.6 0 420 156 103 777.6 0 103 157 76 777.6 0 76 158 102 777.6 0 102 159 129 777.6 0 129 160 153 777.6 0 153 161 244 777.6 0 244 162 482 777.6 0 482 163 121 777.6 0 121 164 179 777.6 0 179 165 400 777.6 0 400 166 195 777.6 0 195 167 387 777.6 0 387 168 359 777.6 0 359 169 251 777.6 0 251 170 377 777.6 0 377 171 193 777.6 0 193 172 379 777.6 0 379 173 742 777.6 0 742 174 1146 777.6 0 1146 175 13051 777.6 0 13051 176 8771 777.6 0 8771 177 15168 777.6 0 15168 178 67 777.6 0 67 179 23 777.6 0 23 180 16 777.6 0 16 181 23 777.6 0 23 182 19 777.6 0 19 183 30 777.6 0 30 184 23 777.6 0 23 185 16 777.6 0 16 186 37 777.6 0 37 187 13 777.6 0 13 188 9 777.6 0 9 189 20 777.6 0 20 190 40 777.6 0 40 191 26 777.6 0 26 192 15 777.6 0 15 193 26 777.6 0 26 194 15 777.6 0 15 195 26 777.6 0 26 196 70 777.6 0 70 197 21 777.6 0 21 198 31 777.6 0 31 199 16 777.6 0 16 200 28 777.6 0 28 201 37 777.6 0 37 202 40 777.6 0 40 203 55 777.6 0 55 204 35 777.6 0 35 205 43 777.6 0 43 206 319 777.6 0 319 207 58 777.6 0 58 208 65 777.6 0 65 209 114 777.6 0 114 210 233 777.6 0 233 211 605 777.6 0 605 212 1211 777.6 0 1211 213 23364 777.6 0 23364 214 4550 777.6 0 4550 215 11427 777.6 0 11427 216 63 777.6 0 63 217 21 777.6 0 21 218 44 777.6 0 44 219 22 777.6 0 22 220 27 777.6 0 27 221 16 777.6 0 16 222 10 777.6 0 10 223 28 777.6 0 28 224 17 777.6 0 17 225 21 777.6 0 21 226 24 777.6 0 24 227 23 777.6 0 23 228 32 777.6 0 32 229 21 777.6 0 21 230 42 777.6 0 42 231 294 777.6 0 294 232 167 777.6 0 167 233 393 777.6 0 393 234 66 777.6 0 66 235 36 777.6 0 36 236 27 777.6 0 27 237 16 777.6 0 16 238 32 777.6 0 32 239 11 777.6 0 11 240 18 777.6 0 18 241 33 777.6 0 33 242 38 777.6 0 38 243 78 777.6 0 78 244 20 777.6 0 20 245 11 777.6 0 11 246 14 777.6 0 14 247 12 777.6 0 12 248 11 777.6 0 11 249 19 777.6 0 19 250 43 777.6 0 43 251 107 777.6 0 107 252 112 777.6 0 112 253 91 777.6 0 91 254 90 777.6 0 90 255 175 777.6 0 175 256 338 777.6 0 338 257 145 777.6 0 145 258 213 777.6 0 213 259 26 777.6 0 26 260 6 777.6 0 6 261 14 777.6 0 14 262 20 777.6 0 20 263 22 777.6 0 22 264 13 777.6 0 13 265 8 777.6 0 8 266 23 777.6 0 23 267 17 777.6 0 17 268 9 777.6 0 9 269 11 777.6 0 11 270 19 777.6 0 19 271 29 777.6 0 29 272 24 777.6 0 24 273 6 777.6 0 6 274 14 777.6 0 14 275 9 777.6 0 9 276 10 777.6 0 10 277 42 777.6 0 42 278 589 777.6 0 589 279 20 777.6 0 20 280 7 777.6 0 7 281 11 777.6 0 11 282 49 777.6 0 49 283 30 777.6 0 30 284 7 777.6 0 7 285 28 777.6 0 28 286 15 777.6 0 15 287 11 777.6 0 11 288 10 777.6 0 10 289 4 777.6 0 4 290 15 777.6 0 15 291 27 777.6 0 27 292 25 777.6 0 25 293 21 777.6 0 21 294 18 777.6 0 18 295 11 777.6 0 11 296 21 777.6 0 21 297 64 777.6 0 64 298 80 777.6 0 80 299 75 777.6 0 75 300 22 777.6 0 22 301 7 777.6 0 7 Finished in 19.14 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 371,436 Reads with adapters: 61,525 (16.6%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 61,507 (16.6%) Total basepairs processed: 81,075,758 bp Total written (filtered): 16,852,600 bp (20.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 61525 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 7 0.0 3 0 0 7 19 295 0.0 3 0 275 9 6 5 20 60973 0.0 4 60034 900 31 2 6 21 120 0.0 4 12 102 3 0 3 22 44 0.0 4 32 0 10 0 2 23 31 0.0 4 1 0 0 29 1 24 5 0.0 4 1 0 0 1 3 41 2 0.0 4 0 0 0 0 2 42 1 0.0 4 1 49 20 0.0 4 0 0 0 0 20 59 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 127 6 0.0 4 0 0 0 0 6 129 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 180 2 0.0 4 0 0 0 0 2 181 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.59 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 61,507 Reads with adapters: 600 (1.0%) Reads written (passing filters): 61,507 (100.0%) Total basepairs processed: 16,852,600 bp Total written (filtered): 16,817,180 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 600 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 20.7% C: 0.7% G: 77.7% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8 961.0 0 8 4 1 240.3 0 1 11 464 0.0 1 2 462 12 7 0.0 1 0 7 152 3 0.0 1 0 3 171 2 0.0 1 2 210 11 0.0 1 9 2 211 1 0.0 1 1 261 101 0.0 1 89 12 262 2 0.0 1 2