This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 100.73 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 112,972 (3.5%) Reads that were too short: 10,307 (0.3%) Reads written (passing filters): 102,665 (3.2%) Total basepairs processed: 958,672,960 bp Total written (filtered): 25,432,269 bp (2.7%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 112972 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 419 777.6 0 419 7 719 777.6 0 719 8 45743 777.6 0 45743 9 10093 777.6 0 10093 10 31 777.6 0 31 11 19 777.6 0 19 12 13 777.6 0 13 13 23 777.6 0 23 14 4 777.6 0 4 15 9 777.6 0 9 16 8 777.6 0 8 17 83 777.6 0 83 18 13 777.6 0 13 19 6 777.6 0 6 20 5 777.6 0 5 21 10 777.6 0 10 22 12 777.6 0 12 23 6 777.6 0 6 24 8 777.6 0 8 25 49 777.6 0 49 26 27 777.6 0 27 27 8 777.6 0 8 28 12 777.6 0 12 29 8 777.6 0 8 30 14 777.6 0 14 31 175 777.6 0 175 32 132 777.6 0 132 33 18 777.6 0 18 34 14 777.6 0 14 35 11 777.6 0 11 36 4 777.6 0 4 37 5 777.6 0 5 38 20 777.6 0 20 39 35 777.6 0 35 40 23 777.6 0 23 41 20 777.6 0 20 42 14 777.6 0 14 43 11 777.6 0 11 44 18 777.6 0 18 45 13 777.6 0 13 46 6 777.6 0 6 47 33 777.6 0 33 48 148 777.6 0 148 49 56 777.6 0 56 50 25 777.6 0 25 51 42 777.6 0 42 52 13 777.6 0 13 53 15 777.6 0 15 54 8 777.6 0 8 55 83 777.6 0 83 56 304 777.6 0 304 57 49 777.6 0 49 58 9 777.6 0 9 59 7 777.6 0 7 60 25 777.6 0 25 61 69 777.6 0 69 62 18 777.6 0 18 63 9 777.6 0 9 64 14 777.6 0 14 65 30 777.6 0 30 66 268 777.6 0 268 67 60 777.6 0 60 68 9 777.6 0 9 69 16 777.6 0 16 70 20 777.6 0 20 71 63 777.6 0 63 72 98 777.6 0 98 73 13 777.6 0 13 74 9 777.6 0 9 75 23 777.6 0 23 76 65 777.6 0 65 77 57 777.6 0 57 78 86 777.6 0 86 79 77 777.6 0 77 80 67 777.6 0 67 81 91 777.6 0 91 82 156 777.6 0 156 83 96 777.6 0 96 84 167 777.6 0 167 85 200 777.6 0 200 86 1275 777.6 0 1275 87 484 777.6 0 484 88 528 777.6 0 528 89 216 777.6 0 216 90 503 777.6 0 503 91 50 777.6 0 50 92 97 777.6 0 97 93 141 777.6 0 141 94 167 777.6 0 167 95 57 777.6 0 57 96 60 777.6 0 60 97 69 777.6 0 69 98 117 777.6 0 117 99 129 777.6 0 129 100 184 777.6 0 184 101 413 777.6 0 413 102 563 777.6 0 563 103 8871 777.6 0 8871 104 12616 777.6 0 12616 105 5030 777.6 0 5030 106 313 777.6 0 313 107 175 777.6 0 175 108 221 777.6 0 221 109 29 777.6 0 29 110 33 777.6 0 33 111 71 777.6 0 71 112 110 777.6 0 110 113 30 777.6 0 30 114 34 777.6 0 34 115 33 777.6 0 33 116 28 777.6 0 28 117 16 777.6 0 16 118 42 777.6 0 42 119 204 777.6 0 204 120 121 777.6 0 121 121 153 777.6 0 153 122 59 777.6 0 59 123 30 777.6 0 30 124 64 777.6 0 64 125 267 777.6 0 267 126 210 777.6 0 210 127 43 777.6 0 43 128 71 777.6 0 71 129 55 777.6 0 55 130 48 777.6 0 48 131 31 777.6 0 31 132 97 777.6 0 97 133 280 777.6 0 280 134 328 777.6 0 328 135 92 777.6 0 92 136 92 777.6 0 92 137 103 777.6 0 103 138 208 777.6 0 208 139 691 777.6 0 691 140 246 777.6 0 246 141 190 777.6 0 190 142 63 777.6 0 63 143 138 777.6 0 138 144 155 777.6 0 155 145 237 777.6 0 237 146 66 777.6 0 66 147 51 777.6 0 51 148 49 777.6 0 49 149 150 777.6 0 150 150 378 777.6 0 378 151 91 777.6 0 91 152 108 777.6 0 108 153 136 777.6 0 136 154 111 777.6 0 111 155 105 777.6 0 105 156 78 777.6 0 78 157 86 777.6 0 86 158 119 777.6 0 119 159 265 777.6 0 265 160 177 777.6 0 177 161 82 777.6 0 82 162 54 777.6 0 54 163 57 777.6 0 57 164 117 777.6 0 117 165 134 777.6 0 134 166 98 777.6 0 98 167 51 777.6 0 51 168 60 777.6 0 60 169 50 777.6 0 50 170 42 777.6 0 42 171 110 777.6 0 110 172 234 777.6 0 234 173 159 777.6 0 159 174 32 777.6 0 32 175 20 777.6 0 20 176 14 777.6 0 14 177 18 777.6 0 18 178 83 777.6 0 83 179 87 777.6 0 87 180 152 777.6 0 152 181 106 777.6 0 106 182 70 777.6 0 70 183 66 777.6 0 66 184 159 777.6 0 159 185 222 777.6 0 222 186 68 777.6 0 68 187 43 777.6 0 43 188 43 777.6 0 43 189 168 777.6 0 168 190 144 777.6 0 144 191 132 777.6 0 132 192 32 777.6 0 32 193 25 777.6 0 25 194 33 777.6 0 33 195 26 777.6 0 26 196 18 777.6 0 18 197 50 777.6 0 50 198 29 777.6 0 29 199 22 777.6 0 22 200 75 777.6 0 75 201 70 777.6 0 70 202 53 777.6 0 53 203 43 777.6 0 43 204 74 777.6 0 74 205 225 777.6 0 225 206 317 777.6 0 317 207 313 777.6 0 313 208 175 777.6 0 175 209 134 777.6 0 134 210 150 777.6 0 150 211 138 777.6 0 138 212 113 777.6 0 113 213 117 777.6 0 117 214 115 777.6 0 115 215 122 777.6 0 122 216 105 777.6 0 105 217 71 777.6 0 71 218 117 777.6 0 117 219 176 777.6 0 176 220 212 777.6 0 212 221 80 777.6 0 80 222 48 777.6 0 48 223 136 777.6 0 136 224 359 777.6 0 359 225 298 777.6 0 298 226 146 777.6 0 146 227 176 777.6 0 176 228 72 777.6 0 72 229 59 777.6 0 59 230 46 777.6 0 46 231 65 777.6 0 65 232 19 777.6 0 19 233 34 777.6 0 34 234 42 777.6 0 42 235 153 777.6 0 153 236 43 777.6 0 43 237 27 777.6 0 27 238 14 777.6 0 14 239 10 777.6 0 10 240 22 777.6 0 22 241 27 777.6 0 27 242 29 777.6 0 29 243 26 777.6 0 26 244 55 777.6 0 55 245 64 777.6 0 64 246 104 777.6 0 104 247 25 777.6 0 25 248 29 777.6 0 29 249 20 777.6 0 20 250 28 777.6 0 28 251 43 777.6 0 43 252 37 777.6 0 37 253 84 777.6 0 84 254 383 777.6 0 383 255 623 777.6 0 623 256 157 777.6 0 157 257 61 777.6 0 61 258 57 777.6 0 57 259 196 777.6 0 196 260 147 777.6 0 147 261 302 777.6 0 302 262 310 777.6 0 310 263 383 777.6 0 383 264 81 777.6 0 81 265 221 777.6 0 221 266 112 777.6 0 112 267 56 777.6 0 56 268 31 777.6 0 31 269 41 777.6 0 41 270 12 777.6 0 12 271 12 777.6 0 12 272 19 777.6 0 19 273 20 777.6 0 20 274 60 777.6 0 60 275 30 777.6 0 30 276 51 777.6 0 51 277 21 777.6 0 21 278 60 777.6 0 60 279 164 777.6 0 164 280 89 777.6 0 89 281 178 777.6 0 178 282 49 777.6 0 49 283 39 777.6 0 39 284 47 777.6 0 47 285 56 777.6 0 56 286 147 777.6 0 147 287 31 777.6 0 31 288 19 777.6 0 19 289 21 777.6 0 21 290 25 777.6 0 25 291 38 777.6 0 38 292 31 777.6 0 31 293 32 777.6 0 32 294 24 777.6 0 24 295 35 777.6 0 35 296 37 777.6 0 37 297 115 777.6 0 115 298 124 777.6 0 124 299 64 777.6 0 64 300 43 777.6 0 43 301 263 777.6 0 263 Finished in 4.89 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 102,665 Reads with adapters: 46,503 (45.3%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 46,471 (45.3%) Total basepairs processed: 25,432,269 bp Total written (filtered): 12,825,096 bp (50.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 46503 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 18 0.0 3 0 0 13 5 16 404 0.0 3 0 360 22 22 17 45591 0.0 3 43901 1427 209 54 18 130 0.0 3 10 95 13 12 19 219 0.0 3 0 1 178 40 20 87 0.0 3 1 1 0 85 21 9 0.0 3 6 1 1 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 37 1 0.0 3 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 1 56 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 217 11 0.0 3 0 0 0 11 221 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.11 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 46,471 Reads with adapters: 2,625 (5.6%) Reads written (passing filters): 46,471 (100.0%) Total basepairs processed: 12,825,096 bp Total written (filtered): 12,817,043 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2625 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.6% C: 7.0% G: 30.9% T: 11.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2463 726.1 0 2463 4 147 181.5 0 147 5 14 45.4 0 14 6 1 11.3 0 1