This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.98 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 72,121 (2.3%) Reads that were too short: 1,766 (0.1%) Reads written (passing filters): 70,355 (2.2%) Total basepairs processed: 958,672,960 bp Total written (filtered): 20,079,135 bp (2.1%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 72121 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4494 777.6 0 4494 7 1042 777.6 0 1042 8 10482 777.6 0 10482 9 48266 777.6 0 48266 10 61 777.6 0 61 11 9 777.6 0 9 12 6 777.6 0 6 13 9 777.6 0 9 14 6 777.6 0 6 15 7 777.6 0 7 16 3 777.6 0 3 17 4 777.6 0 4 18 12 777.6 0 12 19 6 777.6 0 6 20 13 777.6 0 13 21 4 777.6 0 4 22 8 777.6 0 8 23 11 777.6 0 11 24 7 777.6 0 7 25 4 777.6 0 4 26 8 777.6 0 8 27 5 777.6 0 5 28 14 777.6 0 14 29 6 777.6 0 6 30 13 777.6 0 13 31 9 777.6 0 9 32 6 777.6 0 6 33 7 777.6 0 7 34 9 777.6 0 9 35 12 777.6 0 12 36 20 777.6 0 20 37 26 777.6 0 26 38 57 777.6 0 57 39 23 777.6 0 23 40 39 777.6 0 39 41 922 777.6 0 922 42 92 777.6 0 92 43 21 777.6 0 21 44 94 777.6 0 94 45 71 777.6 0 71 46 6 777.6 0 6 47 6 777.6 0 6 48 6 777.6 0 6 49 8 777.6 0 8 50 4 777.6 0 4 51 4 777.6 0 4 52 6 777.6 0 6 53 14 777.6 0 14 54 4 777.6 0 4 55 3 777.6 0 3 56 10 777.6 0 10 57 14 777.6 0 14 58 114 777.6 0 114 59 40 777.6 0 40 60 64 777.6 0 64 61 8 777.6 0 8 62 9 777.6 0 9 63 19 777.6 0 19 64 7 777.6 0 7 65 3 777.6 0 3 66 12 777.6 0 12 67 5 777.6 0 5 68 6 777.6 0 6 69 10 777.6 0 10 70 29 777.6 0 29 71 14 777.6 0 14 72 10 777.6 0 10 73 6 777.6 0 6 74 6 777.6 0 6 75 12 777.6 0 12 76 373 777.6 0 373 77 203 777.6 0 203 78 211 777.6 0 211 79 11 777.6 0 11 80 270 777.6 0 270 81 41 777.6 0 41 82 44 777.6 0 44 83 20 777.6 0 20 84 7 777.6 0 7 86 1 777.6 0 1 87 12 777.6 0 12 88 45 777.6 0 45 89 2 777.6 0 2 90 20 777.6 0 20 91 10 777.6 0 10 92 4 777.6 0 4 93 3 777.6 0 3 94 7 777.6 0 7 95 10 777.6 0 10 96 22 777.6 0 22 97 90 777.6 0 90 98 153 777.6 0 153 99 11 777.6 0 11 100 88 777.6 0 88 101 132 777.6 0 132 102 255 777.6 0 255 103 22 777.6 0 22 104 16 777.6 0 16 105 10 777.6 0 10 106 4 777.6 0 4 107 5 777.6 0 5 108 10 777.6 0 10 109 15 777.6 0 15 110 149 777.6 0 149 111 127 777.6 0 127 112 445 777.6 0 445 113 3 777.6 0 3 114 4 777.6 0 4 115 4 777.6 0 4 116 8 777.6 0 8 117 5 777.6 0 5 118 22 777.6 0 22 119 5 777.6 0 5 120 2 777.6 0 2 121 8 777.6 0 8 122 6 777.6 0 6 123 6 777.6 0 6 124 5 777.6 0 5 125 6 777.6 0 6 126 2 777.6 0 2 127 27 777.6 0 27 128 3 777.6 0 3 129 5 777.6 0 5 130 2 777.6 0 2 131 8 777.6 0 8 132 8 777.6 0 8 133 7 777.6 0 7 134 12 777.6 0 12 135 7 777.6 0 7 136 11 777.6 0 11 137 7 777.6 0 7 138 14 777.6 0 14 139 10 777.6 0 10 140 6 777.6 0 6 141 8 777.6 0 8 142 15 777.6 0 15 143 8 777.6 0 8 144 3 777.6 0 3 145 1 777.6 0 1 146 4 777.6 0 4 147 14 777.6 0 14 148 6 777.6 0 6 150 5 777.6 0 5 151 13 777.6 0 13 152 8 777.6 0 8 153 3 777.6 0 3 154 4 777.6 0 4 155 2 777.6 0 2 156 4 777.6 0 4 157 37 777.6 0 37 158 17 777.6 0 17 159 13 777.6 0 13 160 8 777.6 0 8 161 7 777.6 0 7 162 12 777.6 0 12 163 10 777.6 0 10 164 6 777.6 0 6 165 6 777.6 0 6 166 4 777.6 0 4 167 18 777.6 0 18 168 200 777.6 0 200 169 63 777.6 0 63 170 13 777.6 0 13 171 12 777.6 0 12 172 5 777.6 0 5 173 4 777.6 0 4 174 3 777.6 0 3 175 3 777.6 0 3 176 6 777.6 0 6 177 9 777.6 0 9 178 8 777.6 0 8 179 6 777.6 0 6 180 6 777.6 0 6 181 8 777.6 0 8 182 19 777.6 0 19 183 14 777.6 0 14 184 25 777.6 0 25 185 8 777.6 0 8 186 5 777.6 0 5 187 11 777.6 0 11 188 14 777.6 0 14 189 10 777.6 0 10 190 4 777.6 0 4 191 5 777.6 0 5 192 8 777.6 0 8 193 13 777.6 0 13 194 17 777.6 0 17 195 28 777.6 0 28 196 12 777.6 0 12 197 5 777.6 0 5 198 16 777.6 0 16 199 20 777.6 0 20 200 64 777.6 0 64 201 47 777.6 0 47 202 4 777.6 0 4 203 12 777.6 0 12 204 53 777.6 0 53 205 44 777.6 0 44 206 42 777.6 0 42 207 19 777.6 0 19 208 14 777.6 0 14 209 4 777.6 0 4 210 8 777.6 0 8 211 9 777.6 0 9 212 17 777.6 0 17 213 26 777.6 0 26 214 41 777.6 0 41 215 30 777.6 0 30 216 30 777.6 0 30 217 32 777.6 0 32 218 29 777.6 0 29 219 24 777.6 0 24 220 21 777.6 0 21 221 23 777.6 0 23 222 33 777.6 0 33 223 29 777.6 0 29 224 46 777.6 0 46 225 28 777.6 0 28 226 13 777.6 0 13 227 18 777.6 0 18 228 20 777.6 0 20 229 26 777.6 0 26 230 24 777.6 0 24 231 8 777.6 0 8 232 7 777.6 0 7 233 11 777.6 0 11 234 8 777.6 0 8 235 9 777.6 0 9 236 13 777.6 0 13 237 18 777.6 0 18 238 20 777.6 0 20 239 14 777.6 0 14 240 18 777.6 0 18 241 29 777.6 0 29 242 13 777.6 0 13 243 10 777.6 0 10 244 12 777.6 0 12 245 12 777.6 0 12 246 7 777.6 0 7 247 10 777.6 0 10 248 13 777.6 0 13 249 27 777.6 0 27 250 15 777.6 0 15 251 8 777.6 0 8 252 6 777.6 0 6 253 20 777.6 0 20 254 21 777.6 0 21 255 37 777.6 0 37 256 21 777.6 0 21 257 26 777.6 0 26 258 19 777.6 0 19 259 18 777.6 0 18 260 17 777.6 0 17 261 3 777.6 0 3 262 10 777.6 0 10 263 23 777.6 0 23 264 32 777.6 0 32 265 26 777.6 0 26 266 14 777.6 0 14 267 10 777.6 0 10 268 18 777.6 0 18 269 16 777.6 0 16 270 4 777.6 0 4 271 3 777.6 0 3 272 9 777.6 0 9 273 16 777.6 0 16 274 18 777.6 0 18 275 10 777.6 0 10 276 21 777.6 0 21 277 19 777.6 0 19 278 13 777.6 0 13 279 17 777.6 0 17 280 15 777.6 0 15 281 30 777.6 0 30 282 17 777.6 0 17 283 11 777.6 0 11 284 9 777.6 0 9 285 16 777.6 0 16 286 11 777.6 0 11 287 17 777.6 0 17 288 32 777.6 0 32 289 21 777.6 0 21 290 11 777.6 0 11 291 5 777.6 0 5 292 7 777.6 0 7 293 6 777.6 0 6 294 18 777.6 0 18 295 19 777.6 0 19 296 13 777.6 0 13 297 9 777.6 0 9 298 7 777.6 0 7 299 9 777.6 0 9 300 8 777.6 0 8 301 7 777.6 0 7 Finished in 3.80 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 70,355 Reads with adapters: 51,167 (72.7%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 51,165 (72.7%) Total basepairs processed: 20,079,135 bp Total written (filtered): 13,911,728 bp (69.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 51167 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 9 0.0 3 0 0 7 2 19 181 0.0 3 0 160 3 5 13 20 50154 0.0 4 49504 640 8 2 21 698 0.0 4 575 109 4 9 1 22 10 0.0 4 0 2 8 23 27 0.0 4 1 0 0 24 2 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 1 27 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 38 2 0.0 4 1 0 0 0 1 119 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 30 0.0 4 0 0 0 0 30 128 47 0.0 4 0 0 0 0 47 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.11 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 51,165 Reads with adapters: 688 (1.3%) Reads written (passing filters): 51,165 (100.0%) Total basepairs processed: 13,911,728 bp Total written (filtered): 13,892,838 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 688 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 8.4% G: 87.4% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 799.5 0 4 4 10 199.9 0 10 5 2 50.0 0 2 6 2 12.5 0 2 9 126 0.2 0 3 123 10 248 0.0 1 6 242 11 246 0.0 1 1 245 13 1 0.0 1 0 1 150 3 0.0 1 0 3 175 1 0.0 1 0 1 208 2 0.0 1 0 2 266 40 0.0 1 38 2 267 2 0.0 1 2 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.