This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 107.27 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 864,721 (27.2%) Reads that were too short: 106,395 (3.3%) Reads written (passing filters): 758,326 (23.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 207,690,237 bp (21.7%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 864721 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 172 777.6 0 172 7 9933 777.6 0 9933 8 2143 777.6 0 2143 9 169 777.6 0 169 10 23 777.6 0 23 11 45 777.6 0 45 12 24 777.6 0 24 13 29 777.6 0 29 14 22 777.6 0 22 15 24 777.6 0 24 16 60 777.6 0 60 17 23 777.6 0 23 18 44 777.6 0 44 19 145 777.6 0 145 20 620373 777.6 0 620373 21 47735 777.6 0 47735 22 208 777.6 0 208 23 61 777.6 0 61 24 49 777.6 0 49 25 73 777.6 0 73 26 1032 777.6 0 1032 27 19 777.6 0 19 28 34 777.6 0 34 29 37 777.6 0 37 30 41 777.6 0 41 31 71 777.6 0 71 32 156 777.6 0 156 33 13026 777.6 0 13026 34 708 777.6 0 708 35 91 777.6 0 91 36 50 777.6 0 50 37 41 777.6 0 41 38 76 777.6 0 76 39 71 777.6 0 71 40 70 777.6 0 70 41 96 777.6 0 96 42 66 777.6 0 66 43 59 777.6 0 59 44 103 777.6 0 103 45 45 777.6 0 45 46 85 777.6 0 85 47 313 777.6 0 313 48 65 777.6 0 65 49 76 777.6 0 76 50 78 777.6 0 78 51 86 777.6 0 86 52 77 777.6 0 77 53 157 777.6 0 157 54 94 777.6 0 94 55 135 777.6 0 135 56 185 777.6 0 185 57 170 777.6 0 170 58 200 777.6 0 200 59 171 777.6 0 171 60 246 777.6 0 246 61 258 777.6 0 258 62 264 777.6 0 264 63 276 777.6 0 276 64 239 777.6 0 239 65 215 777.6 0 215 66 271 777.6 0 271 67 353 777.6 0 353 68 308 777.6 0 308 69 412 777.6 0 412 70 400 777.6 0 400 71 534 777.6 0 534 72 400 777.6 0 400 73 485 777.6 0 485 74 438 777.6 0 438 75 1922 777.6 0 1922 76 1251 777.6 0 1251 77 1049 777.6 0 1049 78 994 777.6 0 994 79 6160 777.6 0 6160 80 1558 777.6 0 1558 81 6151 777.6 0 6151 82 474 777.6 0 474 83 423 777.6 0 423 84 458 777.6 0 458 85 1612 777.6 0 1612 86 479 777.6 0 479 87 339 777.6 0 339 88 758 777.6 0 758 89 293 777.6 0 293 90 814 777.6 0 814 91 257 777.6 0 257 92 309 777.6 0 309 93 261 777.6 0 261 94 280 777.6 0 280 95 209 777.6 0 209 96 199 777.6 0 199 97 231 777.6 0 231 98 215 777.6 0 215 99 194 777.6 0 194 100 196 777.6 0 196 101 214 777.6 0 214 102 202 777.6 0 202 103 221 777.6 0 221 104 206 777.6 0 206 105 185 777.6 0 185 106 172 777.6 0 172 107 195 777.6 0 195 108 196 777.6 0 196 109 340 777.6 0 340 110 164 777.6 0 164 111 181 777.6 0 181 112 194 777.6 0 194 113 184 777.6 0 184 114 191 777.6 0 191 115 208 777.6 0 208 116 229 777.6 0 229 117 247 777.6 0 247 118 200 777.6 0 200 119 200 777.6 0 200 120 206 777.6 0 206 121 216 777.6 0 216 122 519 777.6 0 519 123 449 777.6 0 449 124 1075 777.6 0 1075 125 197 777.6 0 197 126 199 777.6 0 199 127 186 777.6 0 186 128 201 777.6 0 201 129 213 777.6 0 213 130 1280 777.6 0 1280 131 982 777.6 0 982 132 780 777.6 0 780 133 187 777.6 0 187 134 225 777.6 0 225 135 221 777.6 0 221 136 1825 777.6 0 1825 137 1291 777.6 0 1291 138 2588 777.6 0 2588 139 450 777.6 0 450 140 274 777.6 0 274 141 216 777.6 0 216 142 147 777.6 0 147 143 111 777.6 0 111 144 132 777.6 0 132 145 166 777.6 0 166 146 157 777.6 0 157 147 175 777.6 0 175 148 167 777.6 0 167 149 134 777.6 0 134 150 138 777.6 0 138 151 139 777.6 0 139 152 185 777.6 0 185 153 191 777.6 0 191 154 133 777.6 0 133 155 142 777.6 0 142 156 141 777.6 0 141 157 193 777.6 0 193 158 211 777.6 0 211 159 184 777.6 0 184 160 153 777.6 0 153 161 161 777.6 0 161 162 119 777.6 0 119 163 122 777.6 0 122 164 115 777.6 0 115 165 180 777.6 0 180 166 168 777.6 0 168 167 188 777.6 0 188 168 143 777.6 0 143 169 109 777.6 0 109 170 123 777.6 0 123 171 135 777.6 0 135 172 123 777.6 0 123 173 147 777.6 0 147 174 105 777.6 0 105 175 131 777.6 0 131 176 128 777.6 0 128 177 105 777.6 0 105 178 116 777.6 0 116 179 109 777.6 0 109 180 119 777.6 0 119 181 115 777.6 0 115 182 148 777.6 0 148 183 112 777.6 0 112 184 111 777.6 0 111 185 141 777.6 0 141 186 161 777.6 0 161 187 189 777.6 0 189 188 153 777.6 0 153 189 148 777.6 0 148 190 229 777.6 0 229 191 122 777.6 0 122 192 132 777.6 0 132 193 127 777.6 0 127 194 177 777.6 0 177 195 125 777.6 0 125 196 155 777.6 0 155 197 184 777.6 0 184 198 131 777.6 0 131 199 364 777.6 0 364 200 328 777.6 0 328 201 296 777.6 0 296 202 214 777.6 0 214 203 195 777.6 0 195 204 155 777.6 0 155 205 150 777.6 0 150 206 118 777.6 0 118 207 120 777.6 0 120 208 125 777.6 0 125 209 145 777.6 0 145 210 172 777.6 0 172 211 155 777.6 0 155 212 269 777.6 0 269 213 176 777.6 0 176 214 257 777.6 0 257 215 175 777.6 0 175 216 179 777.6 0 179 217 158 777.6 0 158 218 151 777.6 0 151 219 178 777.6 0 178 220 195 777.6 0 195 221 241 777.6 0 241 222 238 777.6 0 238 223 340 777.6 0 340 224 462 777.6 0 462 225 448 777.6 0 448 226 855 777.6 0 855 227 1231 777.6 0 1231 228 968 777.6 0 968 229 904 777.6 0 904 230 740 777.6 0 740 231 919 777.6 0 919 232 641 777.6 0 641 233 550 777.6 0 550 234 544 777.6 0 544 235 435 777.6 0 435 236 682 777.6 0 682 237 810 777.6 0 810 238 1884 777.6 0 1884 239 764 777.6 0 764 240 1439 777.6 0 1439 241 1355 777.6 0 1355 242 560 777.6 0 560 243 410 777.6 0 410 244 937 777.6 0 937 245 747 777.6 0 747 246 1643 777.6 0 1643 247 750 777.6 0 750 248 1384 777.6 0 1384 249 1079 777.6 0 1079 250 1058 777.6 0 1058 251 11929 777.6 0 11929 252 7821 777.6 0 7821 253 52137 777.6 0 52137 254 415 777.6 0 415 255 107 777.6 0 107 256 99 777.6 0 99 257 82 777.6 0 82 258 73 777.6 0 73 259 93 777.6 0 93 260 94 777.6 0 94 261 64 777.6 0 64 262 93 777.6 0 93 263 99 777.6 0 99 264 110 777.6 0 110 265 90 777.6 0 90 266 100 777.6 0 100 267 103 777.6 0 103 268 201 777.6 0 201 269 92 777.6 0 92 270 85 777.6 0 85 271 76 777.6 0 76 272 83 777.6 0 83 273 76 777.6 0 76 274 84 777.6 0 84 275 59 777.6 0 59 276 83 777.6 0 83 277 62 777.6 0 62 278 86 777.6 0 86 279 84 777.6 0 84 280 119 777.6 0 119 281 77 777.6 0 77 282 69 777.6 0 69 283 88 777.6 0 88 284 68 777.6 0 68 285 105 777.6 0 105 286 63 777.6 0 63 287 95 777.6 0 95 288 92 777.6 0 92 289 114 777.6 0 114 290 82 777.6 0 82 291 87 777.6 0 87 292 74 777.6 0 74 293 101 777.6 0 101 294 73 777.6 0 73 295 119 777.6 0 119 296 55 777.6 0 55 297 167 777.6 0 167 298 259 777.6 0 259 299 1485 777.6 0 1485 300 73 777.6 0 73 301 45 777.6 0 45 Finished in 36.41 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 758,326 Reads with adapters: 10,486 (1.4%) Reads that were too short: 165 (0.0%) Reads written (passing filters): 10,321 (1.4%) Total basepairs processed: 207,690,237 bp Total written (filtered): 2,807,552 bp (1.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10486 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 63 0.0 3 0 55 5 3 17 9857 0.0 3 9472 310 66 9 18 17 0.0 3 0 14 0 3 19 20 0.0 3 1 0 10 9 20 10 0.0 3 2 0 0 8 29 1 0.0 3 1 37 1 0.0 3 1 53 2 0.0 3 0 0 0 2 55 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 6 0.0 3 0 0 0 6 63 2 0.0 3 0 0 0 2 64 17 0.0 3 0 0 0 17 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 73 2 0.0 3 0 0 0 2 74 6 0.0 3 0 0 0 6 80 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 82 2 0.0 3 0 0 0 2 83 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 1 1 94 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 99 3 0.0 3 0 0 1 2 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 103 3 0.0 3 0 0 1 2 104 3 0.0 3 0 0 0 3 105 2 0.0 3 0 0 0 2 106 4 0.0 3 0 0 0 4 107 1 0.0 3 0 0 0 1 108 6 0.0 3 0 0 0 6 110 1 0.0 3 0 0 0 1 111 3 0.0 3 0 0 0 3 113 3 0.0 3 0 0 0 3 114 4 0.0 3 0 0 0 4 115 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 1 1 123 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 3 0.0 3 0 0 0 3 128 3 0.0 3 0 0 0 3 129 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 134 5 0.0 3 0 0 0 5 135 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 2 0.0 3 0 0 0 2 145 3 0.0 3 0 0 0 3 146 1 0.0 3 0 0 0 1 147 3 0.0 3 0 0 0 3 148 4 0.0 3 0 0 1 3 149 5 0.0 3 0 0 0 5 150 9 0.0 3 0 0 1 8 151 14 0.0 3 0 0 0 14 152 11 0.0 3 0 0 0 11 153 11 0.0 3 0 0 1 10 154 8 0.0 3 0 0 0 8 155 9 0.0 3 0 0 1 8 156 4 0.0 3 0 0 0 4 157 3 0.0 3 0 0 0 3 158 8 0.0 3 0 0 1 7 159 2 0.0 3 0 0 0 2 160 3 0.0 3 0 0 0 3 161 5 0.0 3 0 0 0 5 162 2 0.0 3 0 0 0 2 163 3 0.0 3 0 0 0 3 165 2 0.0 3 0 0 1 1 166 3 0.0 3 0 0 0 3 167 3 0.0 3 0 0 0 3 168 2 0.0 3 0 0 0 2 169 3 0.0 3 0 0 0 3 170 2 0.0 3 0 0 0 2 171 13 0.0 3 0 0 0 13 172 15 0.0 3 0 0 0 15 173 21 0.0 3 0 0 0 21 174 13 0.0 3 0 0 1 12 175 13 0.0 3 0 0 1 12 176 5 0.0 3 0 0 0 5 177 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 179 5 0.0 3 0 0 0 5 180 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 182 4 0.0 3 0 0 0 4 183 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 196 2 0.0 3 0 0 0 2 197 4 0.0 3 0 0 0 4 198 3 0.0 3 0 0 0 3 199 4 0.0 3 0 0 0 4 200 3 0.0 3 0 0 0 3 201 2 0.0 3 0 0 0 2 203 6 0.0 3 0 0 0 6 204 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 206 4 0.0 3 0 0 0 4 207 1 0.0 3 0 0 0 1 208 5 0.0 3 0 0 0 5 209 2 0.0 3 0 0 0 2 210 2 0.0 3 0 0 0 2 211 2 0.0 3 0 0 0 2 212 2 0.0 3 0 0 0 2 213 4 0.0 3 0 0 1 3 214 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 224 2 0.0 3 0 0 0 2 226 4 0.0 3 0 0 0 4 227 3 0.0 3 0 0 0 3 233 2 0.0 3 0 0 0 2 236 3 0.0 3 0 0 0 3 237 3 0.0 3 0 0 0 3 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 245 2 0.0 3 0 0 0 2 247 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 256 2 0.0 3 0 0 0 2 257 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 264 2 0.0 3 0 0 0 2 265 3 0.0 3 0 0 0 3 266 1 0.0 3 0 0 0 1 267 2 0.0 3 0 0 0 2 268 7 0.0 3 0 0 0 7 269 3 0.0 3 0 0 0 3 270 1 0.0 3 0 0 0 1 272 3 0.0 3 0 0 0 3 273 4 0.0 3 0 0 0 4 274 6 0.0 3 0 0 0 6 275 1 0.0 3 0 0 0 1 276 2 0.0 3 0 0 0 2 277 2 0.0 3 0 0 0 2 278 3 0.0 3 0 0 0 3 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 10,321 Reads with adapters: 937 (9.1%) Reads written (passing filters): 10,321 (100.0%) Total basepairs processed: 2,807,552 bp Total written (filtered): 2,804,659 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 937 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.8% C: 52.4% G: 8.5% T: 16.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 856 161.3 0 856 4 80 40.3 0 80 5 1 10.1 0 1