This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 105.60 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 408,841 (12.8%) Reads that were too short: 18,017 (0.6%) Reads written (passing filters): 390,824 (12.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 67,120,308 bp (7.0%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 408841 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 448 777.6 0 448 7 29633 777.6 0 29633 8 9163 777.6 0 9163 9 18 777.6 0 18 10 5 777.6 0 5 11 58 777.6 0 58 12 11 777.6 0 11 13 7 777.6 0 7 14 12 777.6 0 12 15 5 777.6 0 5 16 5 777.6 0 5 17 11 777.6 0 11 18 11 777.6 0 11 19 5 777.6 0 5 20 10 777.6 0 10 21 6 777.6 0 6 22 4 777.6 0 4 23 5 777.6 0 5 24 7 777.6 0 7 25 3 777.6 0 3 26 16 777.6 0 16 27 76 777.6 0 76 28 11 777.6 0 11 29 4 777.6 0 4 30 1 777.6 0 1 31 7 777.6 0 7 32 8 777.6 0 8 33 11 777.6 0 11 34 30 777.6 0 30 35 13 777.6 0 13 36 5 777.6 0 5 37 15 777.6 0 15 38 11 777.6 0 11 39 3 777.6 0 3 40 7 777.6 0 7 41 20 777.6 0 20 42 7 777.6 0 7 43 38 777.6 0 38 44 20 777.6 0 20 45 6 777.6 0 6 46 4 777.6 0 4 47 1 777.6 0 1 48 10 777.6 0 10 49 1 777.6 0 1 50 1 777.6 0 1 51 5 777.6 0 5 52 5 777.6 0 5 53 8 777.6 0 8 54 6 777.6 0 6 55 5 777.6 0 5 56 6 777.6 0 6 57 7 777.6 0 7 58 12 777.6 0 12 59 9 777.6 0 9 60 9 777.6 0 9 61 7 777.6 0 7 62 7 777.6 0 7 63 20 777.6 0 20 64 87 777.6 0 87 65 77 777.6 0 77 66 83 777.6 0 83 67 5 777.6 0 5 68 8 777.6 0 8 69 43 777.6 0 43 70 17 777.6 0 17 71 29 777.6 0 29 72 17 777.6 0 17 73 14 777.6 0 14 74 9 777.6 0 9 75 18 777.6 0 18 76 2 777.6 0 2 77 7 777.6 0 7 78 2 777.6 0 2 79 25 777.6 0 25 80 24 777.6 0 24 81 67 777.6 0 67 82 48 777.6 0 48 83 55 777.6 0 55 84 81 777.6 0 81 85 95 777.6 0 95 86 31 777.6 0 31 87 18 777.6 0 18 88 88 777.6 0 88 89 140 777.6 0 140 90 167 777.6 0 167 91 101 777.6 0 101 92 27 777.6 0 27 93 165 777.6 0 165 94 116 777.6 0 116 95 268 777.6 0 268 96 77 777.6 0 77 97 41 777.6 0 41 98 46 777.6 0 46 99 97 777.6 0 97 100 151 777.6 0 151 101 598 777.6 0 598 102 1098 777.6 0 1098 103 194 777.6 0 194 104 204 777.6 0 204 105 220 777.6 0 220 106 185 777.6 0 185 107 218 777.6 0 218 108 62 777.6 0 62 109 99 777.6 0 99 110 287 777.6 0 287 111 525 777.6 0 525 112 219 777.6 0 219 113 160 777.6 0 160 114 157 777.6 0 157 115 513 777.6 0 513 116 115 777.6 0 115 117 53 777.6 0 53 118 91 777.6 0 91 119 135 777.6 0 135 120 103 777.6 0 103 121 115 777.6 0 115 122 303 777.6 0 303 123 288 777.6 0 288 124 515 777.6 0 515 125 746 777.6 0 746 126 194 777.6 0 194 127 320 777.6 0 320 128 187 777.6 0 187 129 154 777.6 0 154 130 303 777.6 0 303 131 352 777.6 0 352 132 433 777.6 0 433 133 161 777.6 0 161 134 201 777.6 0 201 135 287 777.6 0 287 136 473 777.6 0 473 137 660 777.6 0 660 138 1376 777.6 0 1376 139 2111 777.6 0 2111 140 3972 777.6 0 3972 141 6334 777.6 0 6334 142 123168 777.6 0 123168 143 137947 777.6 0 137947 144 39336 777.6 0 39336 145 415 777.6 0 415 146 236 777.6 0 236 147 433 777.6 0 433 148 209 777.6 0 209 149 276 777.6 0 276 150 93 777.6 0 93 151 57 777.6 0 57 152 92 777.6 0 92 153 100 777.6 0 100 154 78 777.6 0 78 155 548 777.6 0 548 156 244 777.6 0 244 157 440 777.6 0 440 158 316 777.6 0 316 159 174 777.6 0 174 160 301 777.6 0 301 161 469 777.6 0 469 162 7654 777.6 0 7654 163 1652 777.6 0 1652 164 3318 777.6 0 3318 165 158 777.6 0 158 166 121 777.6 0 121 167 460 777.6 0 460 168 288 777.6 0 288 169 433 777.6 0 433 170 129 777.6 0 129 171 75 777.6 0 75 172 51 777.6 0 51 173 73 777.6 0 73 174 86 777.6 0 86 175 148 777.6 0 148 176 113 777.6 0 113 177 162 777.6 0 162 178 193 777.6 0 193 179 166 777.6 0 166 180 194 777.6 0 194 181 104 777.6 0 104 182 103 777.6 0 103 183 158 777.6 0 158 184 258 777.6 0 258 185 260 777.6 0 260 186 144 777.6 0 144 187 143 777.6 0 143 188 151 777.6 0 151 189 306 777.6 0 306 190 453 777.6 0 453 191 149 777.6 0 149 192 117 777.6 0 117 193 142 777.6 0 142 194 66 777.6 0 66 195 84 777.6 0 84 196 91 777.6 0 91 197 142 777.6 0 142 198 382 777.6 0 382 199 185 777.6 0 185 200 32 777.6 0 32 201 22 777.6 0 22 202 41 777.6 0 41 203 137 777.6 0 137 204 356 777.6 0 356 205 370 777.6 0 370 206 223 777.6 0 223 207 166 777.6 0 166 208 86 777.6 0 86 209 95 777.6 0 95 210 239 777.6 0 239 211 173 777.6 0 173 212 181 777.6 0 181 213 190 777.6 0 190 214 389 777.6 0 389 215 423 777.6 0 423 216 316 777.6 0 316 217 300 777.6 0 300 218 260 777.6 0 260 219 263 777.6 0 263 220 69 777.6 0 69 221 70 777.6 0 70 222 81 777.6 0 81 223 207 777.6 0 207 224 598 777.6 0 598 225 245 777.6 0 245 226 159 777.6 0 159 227 254 777.6 0 254 228 160 777.6 0 160 229 145 777.6 0 145 230 175 777.6 0 175 231 86 777.6 0 86 232 84 777.6 0 84 233 84 777.6 0 84 234 214 777.6 0 214 235 260 777.6 0 260 236 220 777.6 0 220 237 177 777.6 0 177 238 93 777.6 0 93 239 87 777.6 0 87 240 64 777.6 0 64 241 39 777.6 0 39 242 53 777.6 0 53 243 122 777.6 0 122 244 266 777.6 0 266 245 418 777.6 0 418 246 277 777.6 0 277 247 132 777.6 0 132 248 46 777.6 0 46 249 21 777.6 0 21 250 27 777.6 0 27 251 83 777.6 0 83 252 154 777.6 0 154 253 331 777.6 0 331 254 236 777.6 0 236 255 227 777.6 0 227 256 249 777.6 0 249 257 227 777.6 0 227 258 248 777.6 0 248 259 129 777.6 0 129 260 78 777.6 0 78 261 89 777.6 0 89 262 207 777.6 0 207 263 370 777.6 0 370 264 361 777.6 0 361 265 209 777.6 0 209 266 255 777.6 0 255 267 135 777.6 0 135 268 190 777.6 0 190 269 100 777.6 0 100 270 75 777.6 0 75 271 52 777.6 0 52 272 100 777.6 0 100 273 262 777.6 0 262 274 188 777.6 0 188 275 131 777.6 0 131 276 108 777.6 0 108 277 75 777.6 0 75 278 82 777.6 0 82 279 315 777.6 0 315 280 122 777.6 0 122 281 146 777.6 0 146 282 110 777.6 0 110 283 131 777.6 0 131 284 288 777.6 0 288 285 237 777.6 0 237 286 229 777.6 0 229 287 132 777.6 0 132 288 67 777.6 0 67 289 47 777.6 0 47 290 30 777.6 0 30 291 88 777.6 0 88 292 492 777.6 0 492 293 309 777.6 0 309 294 244 777.6 0 244 295 158 777.6 0 158 296 74 777.6 0 74 297 82 777.6 0 82 298 57 777.6 0 57 299 55 777.6 0 55 300 208 777.6 0 208 301 304 777.6 0 304 Finished in 17.47 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 390,824 Reads with adapters: 9,615 (2.5%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 9,609 (2.5%) Total basepairs processed: 67,120,308 bp Total written (filtered): 2,602,321 bp (3.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9615 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 0 1 2 19 22 0.0 3 0 21 1 20 9357 0.0 4 9219 133 5 21 23 0.0 4 2 20 0 1 22 4 0.0 4 0 0 4 23 5 0.0 4 0 0 0 4 1 28 1 0.0 4 0 0 0 0 1 37 3 0.0 4 0 0 0 0 3 38 3 0.0 4 0 0 0 0 3 41 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 187 0.0 4 0 0 0 0 187 135 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 1 169 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.40 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 9,609 Reads with adapters: 238 (2.5%) Reads written (passing filters): 9,609 (100.0%) Total basepairs processed: 2,602,321 bp Total written (filtered): 2,598,921 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 238 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 0.4% G: 94.1% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 150.1 0 1 4 2 37.5 0 2 10 12 0.0 1 0 12 11 218 0.0 1 1 217 13 1 0.0 1 0 1 190 2 0.0 1 0 2 209 1 0.0 1 1 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.