This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 104.06 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 75,076 (2.4%) Reads that were too short: 16,794 (0.5%) Reads written (passing filters): 58,282 (1.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 13,497,652 bp (1.4%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 75076 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 437 777.6 0 437 7 7602 777.6 0 7602 8 396 777.6 0 396 9 23191 777.6 0 23191 10 51 777.6 0 51 11 14 777.6 0 14 12 7 777.6 0 7 13 6 777.6 0 6 14 6 777.6 0 6 15 11 777.6 0 11 16 13 777.6 0 13 17 9 777.6 0 9 18 6 777.6 0 6 19 13 777.6 0 13 20 9 777.6 0 9 21 8 777.6 0 8 22 13 777.6 0 13 23 16 777.6 0 16 24 8 777.6 0 8 25 19 777.6 0 19 26 34 777.6 0 34 27 40 777.6 0 40 28 518 777.6 0 518 29 468 777.6 0 468 30 711 777.6 0 711 31 174 777.6 0 174 32 34 777.6 0 34 33 34 777.6 0 34 34 12 777.6 0 12 35 5 777.6 0 5 36 18 777.6 0 18 37 19 777.6 0 19 38 90 777.6 0 90 39 12 777.6 0 12 40 11 777.6 0 11 41 16 777.6 0 16 42 11 777.6 0 11 43 11 777.6 0 11 44 11 777.6 0 11 45 9 777.6 0 9 46 9 777.6 0 9 47 9 777.6 0 9 48 17 777.6 0 17 49 9 777.6 0 9 50 17 777.6 0 17 51 16 777.6 0 16 52 12 777.6 0 12 53 15 777.6 0 15 54 15 777.6 0 15 55 13 777.6 0 13 56 9 777.6 0 9 57 12 777.6 0 12 58 21 777.6 0 21 59 92 777.6 0 92 60 8 777.6 0 8 61 4 777.6 0 4 62 12 777.6 0 12 63 29 777.6 0 29 64 17 777.6 0 17 65 23 777.6 0 23 66 21 777.6 0 21 67 45 777.6 0 45 68 291 777.6 0 291 69 267 777.6 0 267 70 180 777.6 0 180 71 7 777.6 0 7 72 12 777.6 0 12 73 10 777.6 0 10 74 11 777.6 0 11 75 17 777.6 0 17 76 9 777.6 0 9 77 6 777.6 0 6 78 8 777.6 0 8 79 24 777.6 0 24 80 99 777.6 0 99 81 91 777.6 0 91 82 47 777.6 0 47 83 27 777.6 0 27 84 55 777.6 0 55 85 52 777.6 0 52 86 70 777.6 0 70 87 19 777.6 0 19 88 100 777.6 0 100 89 380 777.6 0 380 90 19 777.6 0 19 91 14 777.6 0 14 92 7 777.6 0 7 93 12 777.6 0 12 94 50 777.6 0 50 95 496 777.6 0 496 96 262 777.6 0 262 97 65 777.6 0 65 98 28 777.6 0 28 99 37 777.6 0 37 100 201 777.6 0 201 101 364 777.6 0 364 102 162 777.6 0 162 103 126 777.6 0 126 104 39 777.6 0 39 105 30 777.6 0 30 106 42 777.6 0 42 107 38 777.6 0 38 108 12 777.6 0 12 109 25 777.6 0 25 110 51 777.6 0 51 111 37 777.6 0 37 112 28 777.6 0 28 113 29 777.6 0 29 114 26 777.6 0 26 115 77 777.6 0 77 116 81 777.6 0 81 117 154 777.6 0 154 118 144 777.6 0 144 119 119 777.6 0 119 120 106 777.6 0 106 121 88 777.6 0 88 122 105 777.6 0 105 123 76 777.6 0 76 124 101 777.6 0 101 125 196 777.6 0 196 126 160 777.6 0 160 127 113 777.6 0 113 128 53 777.6 0 53 129 37 777.6 0 37 130 84 777.6 0 84 131 164 777.6 0 164 132 243 777.6 0 243 133 272 777.6 0 272 134 219 777.6 0 219 135 270 777.6 0 270 136 230 777.6 0 230 137 389 777.6 0 389 138 194 777.6 0 194 139 172 777.6 0 172 140 158 777.6 0 158 141 101 777.6 0 101 142 56 777.6 0 56 143 26 777.6 0 26 144 94 777.6 0 94 145 93 777.6 0 93 146 226 777.6 0 226 147 134 777.6 0 134 148 67 777.6 0 67 149 58 777.6 0 58 150 82 777.6 0 82 151 180 777.6 0 180 152 200 777.6 0 200 153 90 777.6 0 90 154 236 777.6 0 236 155 995 777.6 0 995 156 175 777.6 0 175 157 352 777.6 0 352 158 186 777.6 0 186 159 89 777.6 0 89 160 85 777.6 0 85 161 114 777.6 0 114 162 75 777.6 0 75 163 57 777.6 0 57 164 159 777.6 0 159 165 120 777.6 0 120 166 144 777.6 0 144 167 433 777.6 0 433 168 95 777.6 0 95 169 130 777.6 0 130 170 178 777.6 0 178 171 66 777.6 0 66 172 68 777.6 0 68 173 100 777.6 0 100 174 440 777.6 0 440 175 180 777.6 0 180 176 176 777.6 0 176 177 212 777.6 0 212 178 174 777.6 0 174 179 311 777.6 0 311 180 1554 777.6 0 1554 181 1201 777.6 0 1201 182 469 777.6 0 469 183 207 777.6 0 207 184 172 777.6 0 172 185 212 777.6 0 212 186 199 777.6 0 199 187 139 777.6 0 139 188 55 777.6 0 55 189 29 777.6 0 29 190 102 777.6 0 102 191 201 777.6 0 201 192 241 777.6 0 241 193 239 777.6 0 239 194 943 777.6 0 943 195 1043 777.6 0 1043 196 171 777.6 0 171 197 263 777.6 0 263 198 405 777.6 0 405 199 399 777.6 0 399 200 472 777.6 0 472 201 324 777.6 0 324 202 2385 777.6 0 2385 203 1415 777.6 0 1415 204 83 777.6 0 83 205 173 777.6 0 173 206 163 777.6 0 163 207 84 777.6 0 84 208 59 777.6 0 59 209 47 777.6 0 47 210 60 777.6 0 60 211 73 777.6 0 73 212 127 777.6 0 127 213 182 777.6 0 182 214 171 777.6 0 171 215 95 777.6 0 95 216 74 777.6 0 74 217 98 777.6 0 98 218 150 777.6 0 150 219 163 777.6 0 163 220 91 777.6 0 91 221 57 777.6 0 57 222 59 777.6 0 59 223 117 777.6 0 117 224 142 777.6 0 142 225 83 777.6 0 83 226 86 777.6 0 86 227 182 777.6 0 182 228 124 777.6 0 124 229 139 777.6 0 139 230 104 777.6 0 104 231 54 777.6 0 54 232 40 777.6 0 40 233 30 777.6 0 30 234 57 777.6 0 57 235 123 777.6 0 123 236 127 777.6 0 127 237 94 777.6 0 94 238 168 777.6 0 168 239 142 777.6 0 142 240 110 777.6 0 110 241 51 777.6 0 51 242 40 777.6 0 40 243 62 777.6 0 62 244 55 777.6 0 55 245 98 777.6 0 98 246 114 777.6 0 114 247 154 777.6 0 154 248 130 777.6 0 130 249 468 777.6 0 468 250 77 777.6 0 77 251 56 777.6 0 56 252 77 777.6 0 77 253 87 777.6 0 87 254 72 777.6 0 72 255 77 777.6 0 77 256 126 777.6 0 126 257 345 777.6 0 345 258 255 777.6 0 255 259 256 777.6 0 256 260 205 777.6 0 205 261 36 777.6 0 36 262 78 777.6 0 78 263 202 777.6 0 202 264 208 777.6 0 208 265 106 777.6 0 106 266 151 777.6 0 151 267 173 777.6 0 173 268 166 777.6 0 166 269 113 777.6 0 113 270 27 777.6 0 27 271 47 777.6 0 47 272 53 777.6 0 53 273 88 777.6 0 88 274 221 777.6 0 221 275 139 777.6 0 139 276 189 777.6 0 189 277 111 777.6 0 111 278 145 777.6 0 145 279 95 777.6 0 95 280 123 777.6 0 123 281 69 777.6 0 69 282 38 777.6 0 38 283 32 777.6 0 32 284 42 777.6 0 42 285 71 777.6 0 71 286 116 777.6 0 116 287 149 777.6 0 149 288 118 777.6 0 118 289 38 777.6 0 38 290 43 777.6 0 43 291 154 777.6 0 154 292 946 777.6 0 946 293 298 777.6 0 298 294 172 777.6 0 172 295 122 777.6 0 122 296 79 777.6 0 79 297 86 777.6 0 86 298 95 777.6 0 95 299 236 777.6 0 236 300 168 777.6 0 168 301 715 777.6 0 715 Finished in 3.23 s (55 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 58,282 Reads with adapters: 5,220 (9.0%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 5,208 (8.9%) Total basepairs processed: 13,497,652 bp Total written (filtered): 1,418,413 bp (10.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5220 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 25 0.0 3 0 23 0 2 20 5088 0.0 4 5007 78 2 1 21 13 0.0 4 1 12 22 1 0.0 4 1 24 1 0.0 4 1 25 2 0.0 4 0 0 0 0 2 50 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 56 0.0 4 0 0 0 0 56 128 16 0.0 4 0 0 0 0 16 137 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 173 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 5,208 Reads with adapters: 25 (0.5%) Reads written (passing filters): 5,208 (100.0%) Total basepairs processed: 1,418,413 bp Total written (filtered): 1,417,539 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 25 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.0% C: 0.0% G: 88.0% T: 8.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 11 21 0.0 1 0 21 12 1 0.0 1 0 1 152 1 0.0 1 0 1 210 1 0.0 1 0 1 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.