This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.50 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 458,823 (14.4%) Reads that were too short: 23,326 (0.7%) Reads written (passing filters): 435,497 (13.7%) Total basepairs processed: 958,672,960 bp Total written (filtered): 90,957,526 bp (9.5%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 458823 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1521 777.6 0 1521 7 164864 777.6 0 164864 8 99 777.6 0 99 9 1329 777.6 0 1329 10 47 777.6 0 47 11 13 777.6 0 13 12 9 777.6 0 9 13 42 777.6 0 42 14 29 777.6 0 29 15 31 777.6 0 31 16 14 777.6 0 14 17 14 777.6 0 14 18 15 777.6 0 15 19 21 777.6 0 21 20 16 777.6 0 16 21 14 777.6 0 14 22 12 777.6 0 12 23 18 777.6 0 18 24 25 777.6 0 25 25 37 777.6 0 37 26 29 777.6 0 29 27 230 777.6 0 230 28 299 777.6 0 299 29 213 777.6 0 213 30 22 777.6 0 22 31 41 777.6 0 41 32 14 777.6 0 14 33 20 777.6 0 20 34 20 777.6 0 20 35 23 777.6 0 23 36 170 777.6 0 170 37 28 777.6 0 28 38 11 777.6 0 11 39 20 777.6 0 20 40 14 777.6 0 14 41 12 777.6 0 12 42 18 777.6 0 18 43 19 777.6 0 19 44 24 777.6 0 24 45 21 777.6 0 21 46 20 777.6 0 20 47 16 777.6 0 16 48 17 777.6 0 17 49 14 777.6 0 14 50 33 777.6 0 33 51 44 777.6 0 44 52 85 777.6 0 85 53 26 777.6 0 26 54 14 777.6 0 14 55 10 777.6 0 10 56 19 777.6 0 19 57 50 777.6 0 50 58 116 777.6 0 116 59 383 777.6 0 383 60 28 777.6 0 28 61 11 777.6 0 11 62 10 777.6 0 10 63 18 777.6 0 18 64 11 777.6 0 11 65 23 777.6 0 23 66 28 777.6 0 28 67 19 777.6 0 19 68 22 777.6 0 22 69 13 777.6 0 13 70 12 777.6 0 12 71 19 777.6 0 19 72 23 777.6 0 23 73 27 777.6 0 27 74 259 777.6 0 259 75 44 777.6 0 44 76 59 777.6 0 59 77 80 777.6 0 80 78 66 777.6 0 66 79 65 777.6 0 65 80 93 777.6 0 93 81 207 777.6 0 207 82 96 777.6 0 96 83 102 777.6 0 102 84 82 777.6 0 82 85 67 777.6 0 67 86 97 777.6 0 97 87 289 777.6 0 289 88 79 777.6 0 79 89 83 777.6 0 83 90 339 777.6 0 339 91 258 777.6 0 258 92 178 777.6 0 178 93 93 777.6 0 93 94 131 777.6 0 131 95 229 777.6 0 229 96 391 777.6 0 391 97 833 777.6 0 833 98 197 777.6 0 197 99 49 777.6 0 49 100 85 777.6 0 85 101 130 777.6 0 130 102 166 777.6 0 166 103 317 777.6 0 317 104 348 777.6 0 348 105 410 777.6 0 410 106 752 777.6 0 752 107 1796 777.6 0 1796 108 23462 777.6 0 23462 109 9113 777.6 0 9113 110 18226 777.6 0 18226 111 124 777.6 0 124 112 83 777.6 0 83 113 119 777.6 0 119 114 97 777.6 0 97 115 120 777.6 0 120 116 74 777.6 0 74 117 134 777.6 0 134 118 199 777.6 0 199 119 110 777.6 0 110 120 174 777.6 0 174 121 211 777.6 0 211 122 127 777.6 0 127 123 120 777.6 0 120 124 195 777.6 0 195 125 148 777.6 0 148 126 140 777.6 0 140 127 329 777.6 0 329 128 518 777.6 0 518 129 333 777.6 0 333 130 167 777.6 0 167 131 196 777.6 0 196 132 162 777.6 0 162 133 338 777.6 0 338 134 8798 777.6 0 8798 135 1483 777.6 0 1483 136 2415 777.6 0 2415 137 212 777.6 0 212 138 285 777.6 0 285 139 180 777.6 0 180 140 373 777.6 0 373 141 400 777.6 0 400 142 371 777.6 0 371 143 362 777.6 0 362 144 491 777.6 0 491 145 492 777.6 0 492 146 943 777.6 0 943 147 1947 777.6 0 1947 148 655 777.6 0 655 149 1006 777.6 0 1006 150 981 777.6 0 981 151 669 777.6 0 669 152 775 777.6 0 775 153 1171 777.6 0 1171 154 1862 777.6 0 1862 155 11716 777.6 0 11716 156 4106 777.6 0 4106 157 4990 777.6 0 4990 158 1882 777.6 0 1882 159 2298 777.6 0 2298 160 36066 777.6 0 36066 161 57762 777.6 0 57762 162 49695 777.6 0 49695 163 357 777.6 0 357 164 269 777.6 0 269 165 181 777.6 0 181 166 261 777.6 0 261 167 198 777.6 0 198 168 166 777.6 0 166 169 121 777.6 0 121 170 121 777.6 0 121 171 151 777.6 0 151 172 68 777.6 0 68 173 68 777.6 0 68 174 219 777.6 0 219 175 536 777.6 0 536 176 377 777.6 0 377 177 217 777.6 0 217 178 109 777.6 0 109 179 73 777.6 0 73 180 139 777.6 0 139 181 129 777.6 0 129 182 248 777.6 0 248 183 256 777.6 0 256 184 244 777.6 0 244 185 121 777.6 0 121 186 166 777.6 0 166 187 170 777.6 0 170 188 142 777.6 0 142 189 68 777.6 0 68 190 53 777.6 0 53 191 103 777.6 0 103 192 209 777.6 0 209 193 288 777.6 0 288 194 258 777.6 0 258 195 288 777.6 0 288 196 87 777.6 0 87 197 43 777.6 0 43 198 54 777.6 0 54 199 212 777.6 0 212 200 218 777.6 0 218 201 105 777.6 0 105 202 104 777.6 0 104 203 137 777.6 0 137 204 202 777.6 0 202 205 153 777.6 0 153 206 214 777.6 0 214 207 209 777.6 0 209 208 205 777.6 0 205 209 127 777.6 0 127 210 80 777.6 0 80 211 173 777.6 0 173 212 177 777.6 0 177 213 96 777.6 0 96 214 157 777.6 0 157 215 149 777.6 0 149 216 213 777.6 0 213 217 104 777.6 0 104 218 144 777.6 0 144 219 223 777.6 0 223 220 610 777.6 0 610 221 276 777.6 0 276 222 540 777.6 0 540 223 268 777.6 0 268 224 60 777.6 0 60 225 240 777.6 0 240 226 191 777.6 0 191 227 211 777.6 0 211 228 260 777.6 0 260 229 280 777.6 0 280 230 278 777.6 0 278 231 369 777.6 0 369 232 269 777.6 0 269 233 156 777.6 0 156 234 212 777.6 0 212 235 352 777.6 0 352 236 392 777.6 0 392 237 194 777.6 0 194 238 97 777.6 0 97 239 88 777.6 0 88 240 67 777.6 0 67 241 109 777.6 0 109 242 43 777.6 0 43 243 57 777.6 0 57 244 61 777.6 0 61 245 78 777.6 0 78 246 205 777.6 0 205 247 261 777.6 0 261 248 316 777.6 0 316 249 168 777.6 0 168 250 94 777.6 0 94 251 114 777.6 0 114 252 161 777.6 0 161 253 143 777.6 0 143 254 197 777.6 0 197 255 376 777.6 0 376 256 896 777.6 0 896 257 825 777.6 0 825 258 456 777.6 0 456 259 202 777.6 0 202 260 151 777.6 0 151 261 371 777.6 0 371 262 363 777.6 0 363 263 145 777.6 0 145 264 56 777.6 0 56 265 89 777.6 0 89 266 84 777.6 0 84 267 290 777.6 0 290 268 449 777.6 0 449 269 206 777.6 0 206 270 160 777.6 0 160 271 82 777.6 0 82 272 90 777.6 0 90 273 255 777.6 0 255 274 113 777.6 0 113 275 158 777.6 0 158 276 92 777.6 0 92 277 105 777.6 0 105 278 143 777.6 0 143 279 237 777.6 0 237 280 542 777.6 0 542 281 700 777.6 0 700 282 959 777.6 0 959 283 571 777.6 0 571 284 270 777.6 0 270 285 224 777.6 0 224 286 232 777.6 0 232 287 219 777.6 0 219 288 311 777.6 0 311 289 300 777.6 0 300 290 159 777.6 0 159 291 95 777.6 0 95 292 55 777.6 0 55 293 161 777.6 0 161 294 351 777.6 0 351 295 362 777.6 0 362 296 179 777.6 0 179 297 296 777.6 0 296 298 104 777.6 0 104 299 72 777.6 0 72 300 232 777.6 0 232 301 254 777.6 0 254 Finished in 18.54 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 435,497 Reads with adapters: 167,680 (38.5%) Reads that were too short: 137 (0.0%) Reads written (passing filters): 167,543 (38.5%) Total basepairs processed: 90,957,526 bp Total written (filtered): 46,142,071 bp (50.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 167680 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 26 0.0 3 0 0 17 9 16 1012 0.0 3 0 868 85 59 17 164023 0.0 3 158801 4417 642 163 18 333 0.0 3 13 291 19 10 19 227 0.0 3 0 0 149 78 20 318 0.0 3 0 0 0 318 26 1 0.0 3 1 27 1 0.0 3 1 29 1 0.0 3 0 0 0 1 31 1102 0.0 3 0 0 0 1102 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 34 435 0.0 3 0 0 3 432 37 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 57 10 0.0 3 0 0 0 10 58 2 0.0 3 0 0 0 2 60 11 0.0 3 0 0 0 11 61 1 0.0 3 0 0 0 1 63 4 0.0 3 0 0 0 4 64 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 91 4 0.0 3 0 0 0 4 92 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 6 0.0 3 0 0 0 6 120 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 43 0.0 3 0 0 0 43 133 11 0.0 3 0 0 0 11 134 4 0.0 3 0 0 0 4 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 159 29 0.0 3 0 0 0 29 160 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 6 0.0 3 0 0 0 6 181 7 0.0 3 0 0 1 6 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.80 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 167,543 Reads with adapters: 19,612 (11.7%) Reads written (passing filters): 167,543 (100.0%) Total basepairs processed: 46,142,071 bp Total written (filtered): 46,082,077 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 19612 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.6% C: 47.7% G: 7.5% T: 29.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 18478 2617.9 0 18478 4 1118 654.5 0 1118 5 8 163.6 0 8 6 8 40.9 0 8