This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 108.19 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 617,589 (19.4%) Reads that were too short: 25,054 (0.8%) Reads written (passing filters): 592,535 (18.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 167,591,373 bp (17.5%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 617589 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3399 777.6 0 3399 7 44517 777.6 0 44517 8 7456 777.6 0 7456 9 167175 777.6 0 167175 10 2749 777.6 0 2749 11 3959 777.6 0 3959 12 64521 777.6 0 64521 13 130713 777.6 0 130713 14 135925 777.6 0 135925 15 260 777.6 0 260 16 9 777.6 0 9 17 6 777.6 0 6 18 7 777.6 0 7 19 4 777.6 0 4 20 10 777.6 0 10 21 7 777.6 0 7 22 4 777.6 0 4 23 9 777.6 0 9 24 13 777.6 0 13 25 9 777.6 0 9 26 8 777.6 0 8 27 7 777.6 0 7 28 8 777.6 0 8 29 6 777.6 0 6 30 11 777.6 0 11 31 10 777.6 0 10 32 4 777.6 0 4 33 6 777.6 0 6 34 4 777.6 0 4 35 6 777.6 0 6 36 45 777.6 0 45 37 19 777.6 0 19 38 297 777.6 0 297 39 7 777.6 0 7 40 25 777.6 0 25 41 4 777.6 0 4 42 17 777.6 0 17 43 19 777.6 0 19 44 14 777.6 0 14 45 11 777.6 0 11 46 14 777.6 0 14 47 12 777.6 0 12 48 4 777.6 0 4 49 5 777.6 0 5 50 3 777.6 0 3 51 3 777.6 0 3 53 4 777.6 0 4 54 4 777.6 0 4 55 2 777.6 0 2 56 2 777.6 0 2 57 7 777.6 0 7 58 4 777.6 0 4 59 6 777.6 0 6 60 1 777.6 0 1 61 7 777.6 0 7 62 4 777.6 0 4 63 1 777.6 0 1 64 3 777.6 0 3 65 4 777.6 0 4 66 8 777.6 0 8 67 8 777.6 0 8 68 2 777.6 0 2 69 4 777.6 0 4 70 4 777.6 0 4 71 13 777.6 0 13 72 5 777.6 0 5 73 3 777.6 0 3 74 4 777.6 0 4 75 1 777.6 0 1 76 4 777.6 0 4 77 2 777.6 0 2 78 14 777.6 0 14 79 20 777.6 0 20 80 32 777.6 0 32 81 28 777.6 0 28 82 21 777.6 0 21 83 50 777.6 0 50 84 31 777.6 0 31 85 21 777.6 0 21 86 34 777.6 0 34 87 132 777.6 0 132 88 13 777.6 0 13 89 112 777.6 0 112 90 512 777.6 0 512 91 545 777.6 0 545 92 30 777.6 0 30 93 68 777.6 0 68 94 195 777.6 0 195 95 417 777.6 0 417 96 361 777.6 0 361 97 231 777.6 0 231 98 79 777.6 0 79 99 44 777.6 0 44 100 79 777.6 0 79 101 106 777.6 0 106 102 46 777.6 0 46 103 159 777.6 0 159 104 188 777.6 0 188 105 153 777.6 0 153 106 1431 777.6 0 1431 107 399 777.6 0 399 108 129 777.6 0 129 109 118 777.6 0 118 110 367 777.6 0 367 111 152 777.6 0 152 112 188 777.6 0 188 113 197 777.6 0 197 114 65 777.6 0 65 115 88 777.6 0 88 116 72 777.6 0 72 117 33 777.6 0 33 118 51 777.6 0 51 119 117 777.6 0 117 120 498 777.6 0 498 121 216 777.6 0 216 122 374 777.6 0 374 123 92 777.6 0 92 124 116 777.6 0 116 125 136 777.6 0 136 126 481 777.6 0 481 127 1852 777.6 0 1852 128 1440 777.6 0 1440 129 655 777.6 0 655 130 126 777.6 0 126 131 82 777.6 0 82 132 135 777.6 0 135 133 177 777.6 0 177 134 288 777.6 0 288 135 581 777.6 0 581 136 385 777.6 0 385 137 267 777.6 0 267 138 217 777.6 0 217 139 453 777.6 0 453 140 610 777.6 0 610 141 361 777.6 0 361 142 320 777.6 0 320 143 231 777.6 0 231 144 120 777.6 0 120 145 238 777.6 0 238 146 745 777.6 0 745 147 202 777.6 0 202 148 215 777.6 0 215 149 60 777.6 0 60 150 94 777.6 0 94 151 786 777.6 0 786 152 375 777.6 0 375 153 782 777.6 0 782 154 271 777.6 0 271 155 254 777.6 0 254 156 111 777.6 0 111 157 151 777.6 0 151 158 706 777.6 0 706 159 336 777.6 0 336 160 308 777.6 0 308 161 580 777.6 0 580 162 178 777.6 0 178 163 243 777.6 0 243 164 136 777.6 0 136 165 233 777.6 0 233 166 262 777.6 0 262 167 208 777.6 0 208 168 123 777.6 0 123 169 74 777.6 0 74 170 155 777.6 0 155 171 81 777.6 0 81 172 123 777.6 0 123 173 212 777.6 0 212 174 521 777.6 0 521 175 180 777.6 0 180 176 125 777.6 0 125 177 77 777.6 0 77 178 60 777.6 0 60 179 201 777.6 0 201 180 192 777.6 0 192 181 323 777.6 0 323 182 232 777.6 0 232 183 222 777.6 0 222 184 168 777.6 0 168 185 255 777.6 0 255 186 215 777.6 0 215 187 137 777.6 0 137 188 92 777.6 0 92 189 73 777.6 0 73 190 186 777.6 0 186 191 306 777.6 0 306 192 554 777.6 0 554 193 151 777.6 0 151 194 259 777.6 0 259 195 73 777.6 0 73 196 64 777.6 0 64 197 108 777.6 0 108 198 295 777.6 0 295 199 223 777.6 0 223 200 116 777.6 0 116 201 116 777.6 0 116 202 162 777.6 0 162 203 281 777.6 0 281 204 286 777.6 0 286 205 99 777.6 0 99 206 258 777.6 0 258 207 296 777.6 0 296 208 239 777.6 0 239 209 190 777.6 0 190 210 119 777.6 0 119 211 139 777.6 0 139 212 145 777.6 0 145 213 157 777.6 0 157 214 314 777.6 0 314 215 258 777.6 0 258 216 194 777.6 0 194 217 167 777.6 0 167 218 155 777.6 0 155 219 180 777.6 0 180 220 384 777.6 0 384 221 167 777.6 0 167 222 114 777.6 0 114 223 108 777.6 0 108 224 420 777.6 0 420 225 426 777.6 0 426 226 285 777.6 0 285 227 235 777.6 0 235 228 215 777.6 0 215 229 141 777.6 0 141 230 110 777.6 0 110 231 138 777.6 0 138 232 113 777.6 0 113 233 208 777.6 0 208 234 271 777.6 0 271 235 362 777.6 0 362 236 357 777.6 0 357 237 162 777.6 0 162 238 111 777.6 0 111 239 88 777.6 0 88 240 217 777.6 0 217 241 225 777.6 0 225 242 241 777.6 0 241 243 297 777.6 0 297 244 255 777.6 0 255 245 248 777.6 0 248 246 191 777.6 0 191 247 187 777.6 0 187 248 98 777.6 0 98 249 85 777.6 0 85 250 39 777.6 0 39 251 185 777.6 0 185 252 261 777.6 0 261 253 349 777.6 0 349 254 202 777.6 0 202 255 210 777.6 0 210 256 311 777.6 0 311 257 247 777.6 0 247 258 145 777.6 0 145 259 97 777.6 0 97 260 138 777.6 0 138 261 261 777.6 0 261 262 544 777.6 0 544 263 387 777.6 0 387 264 225 777.6 0 225 265 196 777.6 0 196 266 239 777.6 0 239 267 861 777.6 0 861 268 494 777.6 0 494 269 285 777.6 0 285 270 96 777.6 0 96 271 125 777.6 0 125 272 162 777.6 0 162 273 148 777.6 0 148 274 255 777.6 0 255 275 199 777.6 0 199 276 206 777.6 0 206 277 547 777.6 0 547 278 737 777.6 0 737 279 475 777.6 0 475 280 829 777.6 0 829 281 639 777.6 0 639 282 539 777.6 0 539 283 489 777.6 0 489 284 349 777.6 0 349 285 235 777.6 0 235 286 248 777.6 0 248 287 262 777.6 0 262 288 469 777.6 0 469 289 245 777.6 0 245 290 136 777.6 0 136 291 129 777.6 0 129 292 151 777.6 0 151 293 280 777.6 0 280 294 309 777.6 0 309 295 224 777.6 0 224 296 202 777.6 0 202 297 106 777.6 0 106 298 60 777.6 0 60 299 74 777.6 0 74 300 146 777.6 0 146 301 209 777.6 0 209 Finished in 35.45 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 592,535 Reads with adapters: 178,432 (30.1%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 178,403 (30.1%) Total basepairs processed: 167,591,373 bp Total written (filtered): 47,966,295 bp (28.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 178432 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 3 0.0 3 0 0 0 0 3 17 1 0.0 3 0 0 0 0 1 18 12 0.0 3 0 0 11 1 19 431 0.0 3 0 395 6 6 24 20 172021 0.0 4 169572 2364 64 13 8 21 367 0.0 4 52 308 7 22 35 0.0 4 5 0 24 3 3 23 139 0.0 4 74 0 0 61 4 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 1 26 2 0.0 4 2 30 1 0.0 4 0 1 42 1 0.0 4 0 1 43 2 0.0 4 2 45 13 0.0 4 0 0 0 0 13 66 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 120 7 0.0 4 0 0 0 0 7 121 49 0.0 4 0 0 0 0 49 122 4688 0.0 4 0 0 0 0 4688 123 9 0.0 4 0 0 0 0 9 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 563 0.0 4 0 0 0 0 563 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 141 16 0.0 4 0 0 0 0 16 146 1 0.0 4 0 0 0 0 1 147 3 0.0 4 0 0 0 0 3 148 23 0.0 4 0 0 0 0 23 150 2 0.0 4 0 0 0 0 2 177 2 0.0 4 0 0 0 0 2 199 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 223 5 0.0 4 0 0 0 0 5 227 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 258 3 0.0 4 0 0 0 0 3 261 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.49 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 178,403 Reads with adapters: 2,668 (1.5%) Reads written (passing filters): 178,403 (100.0%) Total basepairs processed: 47,966,295 bp Total written (filtered): 47,830,508 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2668 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 17.8% C: 2.0% G: 78.7% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 2787.5 0 11 4 12 696.9 0 12 5 2 174.2 0 2 6 3 43.6 0 3 9 294 0.7 0 9 285 10 1032 0.2 1 31 1001 11 864 0.0 1 1 863 12 2 0.0 1 0 2 13 4 0.0 1 0 4 27 1 0.0 1 0 1 125 1 0.0 1 0 1 129 1 0.0 1 1 138 1 0.0 1 1 150 5 0.0 1 0 5 151 1 0.0 1 0 1 160 1 0.0 1 1 189 1 0.0 1 0 1 208 2 0.0 1 1 1 257 285 0.0 1 267 18 258 15 0.0 1 13 2 259 3 0.0 1 3 260 3 0.0 1 3 261 118 0.0 1 112 6 262 4 0.0 1 4 263 1 0.0 1 1 266 1 0.0 1 1