This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.37 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 373,781 (11.7%) Reads that were too short: 7,622 (0.2%) Reads written (passing filters): 366,159 (11.5%) Total basepairs processed: 958,672,960 bp Total written (filtered): 69,042,129 bp (7.2%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 373781 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 101 777.6 0 101 7 134 777.6 0 134 8 4018 777.6 0 4018 9 7112 777.6 0 7112 10 21 777.6 0 21 11 22 777.6 0 22 12 18 777.6 0 18 13 24 777.6 0 24 14 25 777.6 0 25 15 43 777.6 0 43 16 17 777.6 0 17 17 43 777.6 0 43 18 33 777.6 0 33 19 58 777.6 0 58 20 16 777.6 0 16 21 18 777.6 0 18 22 16 777.6 0 16 23 24 777.6 0 24 24 15 777.6 0 15 25 122 777.6 0 122 26 299 777.6 0 299 27 110 777.6 0 110 28 13 777.6 0 13 29 17 777.6 0 17 30 17 777.6 0 17 31 18 777.6 0 18 32 38 777.6 0 38 33 15 777.6 0 15 34 24 777.6 0 24 35 49 777.6 0 49 36 64 777.6 0 64 37 3352 777.6 0 3352 38 174 777.6 0 174 39 20 777.6 0 20 40 21 777.6 0 21 41 9 777.6 0 9 42 19 777.6 0 19 43 12 777.6 0 12 44 23 777.6 0 23 45 14 777.6 0 14 46 15 777.6 0 15 47 22 777.6 0 22 48 11 777.6 0 11 49 6 777.6 0 6 50 13 777.6 0 13 51 16 777.6 0 16 52 16 777.6 0 16 53 12 777.6 0 12 54 17 777.6 0 17 55 31 777.6 0 31 56 63 777.6 0 63 57 21 777.6 0 21 58 60 777.6 0 60 59 14 777.6 0 14 60 22 777.6 0 22 61 28 777.6 0 28 62 13 777.6 0 13 63 14 777.6 0 14 64 11 777.6 0 11 65 25 777.6 0 25 66 24 777.6 0 24 67 39 777.6 0 39 68 35 777.6 0 35 69 45 777.6 0 45 70 461 777.6 0 461 71 307 777.6 0 307 72 870 777.6 0 870 73 24 777.6 0 24 74 13 777.6 0 13 75 17 777.6 0 17 76 63 777.6 0 63 77 50 777.6 0 50 78 50 777.6 0 50 79 66 777.6 0 66 80 73 777.6 0 73 81 56 777.6 0 56 82 432 777.6 0 432 83 469 777.6 0 469 84 330 777.6 0 330 85 73 777.6 0 73 86 501 777.6 0 501 87 42 777.6 0 42 88 30 777.6 0 30 89 52 777.6 0 52 90 31 777.6 0 31 91 45 777.6 0 45 92 130 777.6 0 130 93 169 777.6 0 169 94 79 777.6 0 79 95 225 777.6 0 225 96 513 777.6 0 513 97 363 777.6 0 363 98 470 777.6 0 470 99 435 777.6 0 435 100 221 777.6 0 221 101 371 777.6 0 371 102 223 777.6 0 223 103 328 777.6 0 328 104 103 777.6 0 103 105 144 777.6 0 144 106 281 777.6 0 281 107 376 777.6 0 376 108 740 777.6 0 740 109 1055 777.6 0 1055 110 1623 777.6 0 1623 111 3234 777.6 0 3234 112 4230 777.6 0 4230 113 127707 777.6 0 127707 114 76361 777.6 0 76361 115 70822 777.6 0 70822 116 427 777.6 0 427 117 117 777.6 0 117 118 179 777.6 0 179 119 86 777.6 0 86 120 110 777.6 0 110 121 265 777.6 0 265 122 425 777.6 0 425 123 127 777.6 0 127 124 177 777.6 0 177 125 271 777.6 0 271 126 141 777.6 0 141 127 213 777.6 0 213 128 190 777.6 0 190 129 245 777.6 0 245 130 365 777.6 0 365 131 247 777.6 0 247 132 318 777.6 0 318 133 655 777.6 0 655 134 7864 777.6 0 7864 135 23496 777.6 0 23496 136 4258 777.6 0 4258 137 8493 777.6 0 8493 138 118 777.6 0 118 139 191 777.6 0 191 140 263 777.6 0 263 141 70 777.6 0 70 142 31 777.6 0 31 143 252 777.6 0 252 144 123 777.6 0 123 145 150 777.6 0 150 146 41 777.6 0 41 147 41 777.6 0 41 148 75 777.6 0 75 149 42 777.6 0 42 150 57 777.6 0 57 151 38 777.6 0 38 152 39 777.6 0 39 153 47 777.6 0 47 154 54 777.6 0 54 155 53 777.6 0 53 156 44 777.6 0 44 157 50 777.6 0 50 158 225 777.6 0 225 159 207 777.6 0 207 160 175 777.6 0 175 161 55 777.6 0 55 162 42 777.6 0 42 163 39 777.6 0 39 164 78 777.6 0 78 165 64 777.6 0 64 166 64 777.6 0 64 167 58 777.6 0 58 168 83 777.6 0 83 169 32 777.6 0 32 170 41 777.6 0 41 171 30 777.6 0 30 172 34 777.6 0 34 173 55 777.6 0 55 174 15 777.6 0 15 175 16 777.6 0 16 176 27 777.6 0 27 177 144 777.6 0 144 178 99 777.6 0 99 179 1136 777.6 0 1136 180 869 777.6 0 869 181 41 777.6 0 41 182 270 777.6 0 270 183 42 777.6 0 42 184 70 777.6 0 70 185 65 777.6 0 65 186 29 777.6 0 29 187 13 777.6 0 13 188 39 777.6 0 39 189 49 777.6 0 49 190 73 777.6 0 73 191 56 777.6 0 56 192 39 777.6 0 39 193 26 777.6 0 26 194 26 777.6 0 26 195 25 777.6 0 25 196 43 777.6 0 43 197 67 777.6 0 67 198 35 777.6 0 35 199 18 777.6 0 18 200 26 777.6 0 26 201 52 777.6 0 52 202 78 777.6 0 78 203 123 777.6 0 123 204 80 777.6 0 80 205 112 777.6 0 112 206 105 777.6 0 105 207 58 777.6 0 58 208 47 777.6 0 47 209 94 777.6 0 94 210 58 777.6 0 58 211 91 777.6 0 91 212 73 777.6 0 73 213 59 777.6 0 59 214 82 777.6 0 82 215 99 777.6 0 99 216 120 777.6 0 120 217 98 777.6 0 98 218 41 777.6 0 41 219 25 777.6 0 25 220 30 777.6 0 30 221 40 777.6 0 40 222 46 777.6 0 46 223 137 777.6 0 137 224 122 777.6 0 122 225 186 777.6 0 186 226 153 777.6 0 153 227 70 777.6 0 70 228 49 777.6 0 49 229 42 777.6 0 42 230 25 777.6 0 25 231 25 777.6 0 25 232 29 777.6 0 29 233 47 777.6 0 47 234 123 777.6 0 123 235 80 777.6 0 80 236 43 777.6 0 43 237 31 777.6 0 31 238 22 777.6 0 22 239 48 777.6 0 48 240 23 777.6 0 23 241 53 777.6 0 53 242 47 777.6 0 47 243 72 777.6 0 72 244 105 777.6 0 105 245 96 777.6 0 96 246 62 777.6 0 62 247 32 777.6 0 32 248 30 777.6 0 30 249 16 777.6 0 16 250 68 777.6 0 68 251 70 777.6 0 70 252 82 777.6 0 82 253 98 777.6 0 98 254 117 777.6 0 117 255 96 777.6 0 96 256 35 777.6 0 35 257 27 777.6 0 27 258 42 777.6 0 42 259 22 777.6 0 22 260 31 777.6 0 31 261 19 777.6 0 19 262 40 777.6 0 40 263 66 777.6 0 66 264 56 777.6 0 56 265 126 777.6 0 126 266 66 777.6 0 66 267 41 777.6 0 41 268 28 777.6 0 28 269 63 777.6 0 63 270 169 777.6 0 169 271 198 777.6 0 198 272 132 777.6 0 132 273 556 777.6 0 556 274 50 777.6 0 50 275 45 777.6 0 45 276 39 777.6 0 39 277 45 777.6 0 45 278 90 777.6 0 90 279 68 777.6 0 68 280 84 777.6 0 84 281 172 777.6 0 172 282 252 777.6 0 252 283 66 777.6 0 66 284 48 777.6 0 48 285 40 777.6 0 40 286 39 777.6 0 39 287 26 777.6 0 26 288 39 777.6 0 39 289 29 777.6 0 29 290 106 777.6 0 106 291 117 777.6 0 117 292 135 777.6 0 135 293 65 777.6 0 65 294 45 777.6 0 45 295 56 777.6 0 56 296 66 777.6 0 66 297 79 777.6 0 79 298 113 777.6 0 113 299 62 777.6 0 62 300 62 777.6 0 62 301 9 777.6 0 9 Finished in 15.51 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 366,159 Reads with adapters: 4,680 (1.3%) Reads that were too short: 702 (0.2%) Reads written (passing filters): 3,978 (1.1%) Total basepairs processed: 69,042,129 bp Total written (filtered): 1,091,544 bp (1.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4680 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 33 0.0 3 0 27 5 1 17 3888 0.0 3 3826 50 10 2 18 4 0.0 3 0 1 2 1 19 11 0.0 3 0 0 3 8 31 1 0.0 3 0 1 33 6 0.0 3 0 0 0 6 47 1 0.0 3 0 0 0 1 48 1 0.0 3 1 49 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 58 8 0.0 3 0 0 0 8 59 1 0.0 3 0 0 1 67 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 4 0.0 3 0 0 0 4 99 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 310 0.0 3 0 1 12 297 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 7 0.0 3 0 0 0 7 116 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 174 2 0.0 3 0 0 0 2 175 72 0.0 3 0 0 2 70 176 199 0.0 3 0 0 1 198 177 59 0.0 3 0 0 0 59 179 4 0.0 3 0 0 0 4 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 11 0.0 3 0 0 0 11 183 8 0.0 3 0 0 0 8 184 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,978 Reads with adapters: 341 (8.6%) Reads written (passing filters): 3,978 (100.0%) Total basepairs processed: 1,091,544 bp Total written (filtered): 1,090,447 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 341 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.2% C: 15.8% G: 12.6% T: 14.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 277 62.2 0 277 4 59 15.5 0 59 5 2 3.9 0 2 6 2 1.0 0 2 8 1 0.1 0 1