This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.14 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 107,972 (3.4%) Reads that were too short: 73,935 (2.3%) Reads written (passing filters): 34,037 (1.1%) Total basepairs processed: 958,672,960 bp Total written (filtered): 7,482,116 bp (0.8%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 107972 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 72 777.6 0 72 7 177 777.6 0 177 8 7161 777.6 0 7161 9 4014 777.6 0 4014 10 15 777.6 0 15 11 2 777.6 0 2 12 23 777.6 0 23 13 2 777.6 0 2 14 1 777.6 0 1 15 1 777.6 0 1 18 5 777.6 0 5 19 3 777.6 0 3 20 1 777.6 0 1 21 373 777.6 0 373 22 20 777.6 0 20 23 4 777.6 0 4 24 2 777.6 0 2 26 2 777.6 0 2 27 4 777.6 0 4 28 3 777.6 0 3 29 3 777.6 0 3 30 1 777.6 0 1 31 4 777.6 0 4 32 5 777.6 0 5 33 9 777.6 0 9 34 1 777.6 0 1 35 5 777.6 0 5 36 6 777.6 0 6 37 50 777.6 0 50 38 13 777.6 0 13 39 15 777.6 0 15 40 6 777.6 0 6 41 2 777.6 0 2 42 1 777.6 0 1 43 11 777.6 0 11 44 3 777.6 0 3 45 3 777.6 0 3 46 9 777.6 0 9 47 9 777.6 0 9 48 14 777.6 0 14 49 15 777.6 0 15 50 3 777.6 0 3 51 7 777.6 0 7 52 10 777.6 0 10 53 7 777.6 0 7 54 6 777.6 0 6 55 10 777.6 0 10 56 107 777.6 0 107 57 53 777.6 0 53 58 3256 777.6 0 3256 59 263 777.6 0 263 60 8 777.6 0 8 61 12 777.6 0 12 62 17 777.6 0 17 63 13 777.6 0 13 64 56 777.6 0 56 65 13 777.6 0 13 66 7 777.6 0 7 67 11 777.6 0 11 68 8 777.6 0 8 69 29 777.6 0 29 70 172 777.6 0 172 71 62 777.6 0 62 72 13 777.6 0 13 73 15 777.6 0 15 74 18 777.6 0 18 75 22 777.6 0 22 76 78 777.6 0 78 77 17 777.6 0 17 78 20 777.6 0 20 79 34 777.6 0 34 80 35 777.6 0 35 81 26 777.6 0 26 82 40 777.6 0 40 83 34 777.6 0 34 84 51 777.6 0 51 85 47 777.6 0 47 86 43 777.6 0 43 87 28 777.6 0 28 88 97 777.6 0 97 89 1912 777.6 0 1912 90 1347 777.6 0 1347 91 1629 777.6 0 1629 92 15 777.6 0 15 93 6 777.6 0 6 94 5 777.6 0 5 95 31 777.6 0 31 96 42 777.6 0 42 97 30 777.6 0 30 98 27 777.6 0 27 99 32 777.6 0 32 100 240 777.6 0 240 101 48 777.6 0 48 102 231 777.6 0 231 103 19 777.6 0 19 104 9 777.6 0 9 105 38 777.6 0 38 106 15 777.6 0 15 107 9 777.6 0 9 108 16 777.6 0 16 109 7 777.6 0 7 110 63 777.6 0 63 111 41 777.6 0 41 112 70 777.6 0 70 113 14 777.6 0 14 114 28 777.6 0 28 115 54 777.6 0 54 116 48 777.6 0 48 117 61 777.6 0 61 118 25 777.6 0 25 119 26 777.6 0 26 120 36 777.6 0 36 121 16 777.6 0 16 122 43 777.6 0 43 123 16 777.6 0 16 124 17 777.6 0 17 125 23 777.6 0 23 126 38 777.6 0 38 127 21 777.6 0 21 128 12 777.6 0 12 129 122 777.6 0 122 130 20 777.6 0 20 131 25 777.6 0 25 132 681 777.6 0 681 133 261 777.6 0 261 134 633 777.6 0 633 135 40 777.6 0 40 136 14 777.6 0 14 137 26 777.6 0 26 138 19 777.6 0 19 139 26 777.6 0 26 140 63 777.6 0 63 141 30 777.6 0 30 142 20 777.6 0 20 143 15 777.6 0 15 144 10 777.6 0 10 145 22 777.6 0 22 146 17 777.6 0 17 147 93 777.6 0 93 148 680 777.6 0 680 149 921 777.6 0 921 150 617 777.6 0 617 151 13 777.6 0 13 152 6 777.6 0 6 153 13 777.6 0 13 154 11 777.6 0 11 155 17 777.6 0 17 156 12 777.6 0 12 157 36 777.6 0 36 158 28 777.6 0 28 159 28 777.6 0 28 160 33 777.6 0 33 161 24 777.6 0 24 162 23 777.6 0 23 163 17 777.6 0 17 164 16 777.6 0 16 165 66 777.6 0 66 166 77 777.6 0 77 167 91 777.6 0 91 168 562 777.6 0 562 169 185 777.6 0 185 170 99 777.6 0 99 171 14 777.6 0 14 172 24 777.6 0 24 173 42 777.6 0 42 174 64 777.6 0 64 175 137 777.6 0 137 176 2536 777.6 0 2536 177 443 777.6 0 443 178 1332 777.6 0 1332 179 29 777.6 0 29 180 16 777.6 0 16 181 11 777.6 0 11 182 20 777.6 0 20 183 22 777.6 0 22 184 41 777.6 0 41 185 46 777.6 0 46 186 36 777.6 0 36 187 16 777.6 0 16 188 6 777.6 0 6 189 13 777.6 0 13 190 18 777.6 0 18 191 14 777.6 0 14 192 19 777.6 0 19 193 69 777.6 0 69 194 30 777.6 0 30 195 15 777.6 0 15 196 13 777.6 0 13 197 16 777.6 0 16 198 35 777.6 0 35 199 40 777.6 0 40 200 13 777.6 0 13 201 17 777.6 0 17 202 16 777.6 0 16 203 37 777.6 0 37 204 38 777.6 0 38 205 51 777.6 0 51 206 56 777.6 0 56 207 310 777.6 0 310 208 247 777.6 0 247 209 126 777.6 0 126 210 74 777.6 0 74 211 34 777.6 0 34 212 44 777.6 0 44 213 71 777.6 0 71 214 39 777.6 0 39 215 99 777.6 0 99 216 135 777.6 0 135 217 114 777.6 0 114 218 196 777.6 0 196 219 408 777.6 0 408 220 5759 777.6 0 5759 221 2755 777.6 0 2755 222 3175 777.6 0 3175 223 31 777.6 0 31 224 27 777.6 0 27 225 96 777.6 0 96 226 213 777.6 0 213 227 99 777.6 0 99 228 50 777.6 0 50 229 44 777.6 0 44 230 51 777.6 0 51 231 36 777.6 0 36 232 17 777.6 0 17 233 26 777.6 0 26 234 21 777.6 0 21 235 29 777.6 0 29 236 52 777.6 0 52 237 64 777.6 0 64 238 62 777.6 0 62 239 93 777.6 0 93 240 75 777.6 0 75 241 123 777.6 0 123 242 33 777.6 0 33 243 19 777.6 0 19 244 40 777.6 0 40 245 55 777.6 0 55 246 71 777.6 0 71 247 49 777.6 0 49 248 33 777.6 0 33 249 20 777.6 0 20 250 24 777.6 0 24 251 15 777.6 0 15 252 26 777.6 0 26 253 82 777.6 0 82 254 78 777.6 0 78 255 207 777.6 0 207 256 219 777.6 0 219 257 154 777.6 0 154 258 43 777.6 0 43 259 39 777.6 0 39 260 38 777.6 0 38 261 74 777.6 0 74 262 33 777.6 0 33 263 72 777.6 0 72 264 75 777.6 0 75 265 96 777.6 0 96 266 183 777.6 0 183 267 102 777.6 0 102 268 178 777.6 0 178 269 278 777.6 0 278 270 277 777.6 0 277 271 160 777.6 0 160 272 116 777.6 0 116 273 110 777.6 0 110 274 1190 777.6 0 1190 275 744 777.6 0 744 276 671 777.6 0 671 277 52 777.6 0 52 278 98 777.6 0 98 279 194 777.6 0 194 280 385 777.6 0 385 281 1370 777.6 0 1370 282 1960 777.6 0 1960 283 29047 777.6 0 29047 284 7016 777.6 0 7016 285 11671 777.6 0 11671 286 765 777.6 0 765 287 44 777.6 0 44 288 78 777.6 0 78 289 64 777.6 0 64 290 160 777.6 0 160 291 42 777.6 0 42 292 28 777.6 0 28 293 96 777.6 0 96 294 77 777.6 0 77 295 53 777.6 0 53 296 72 777.6 0 72 297 10 777.6 0 10 298 12 777.6 0 12 299 9 777.6 0 9 300 6 777.6 0 6 301 29 777.6 0 29 Finished in 1.82 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 34,037 Reads with adapters: 4,164 (12.2%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 4,161 (12.2%) Total basepairs processed: 7,482,116 bp Total written (filtered): 1,125,526 bp (15.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4164 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 27 0.0 3 0 25 1 0 1 20 4036 0.0 4 3995 40 1 21 11 0.0 4 0 10 1 22 2 0.0 4 0 0 1 0 1 23 22 0.0 4 0 0 0 22 24 6 0.0 4 0 0 0 0 6 25 1 0.0 4 1 31 1 0.0 4 1 37 1 0.0 4 1 53 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 127 19 0.0 4 0 0 0 0 19 131 3 0.0 4 0 0 0 0 3 146 23 0.0 4 0 0 0 0 23 153 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 4,161 Reads with adapters: 3,264 (78.4%) Reads written (passing filters): 4,161 (100.0%) Total basepairs processed: 1,125,526 bp Total written (filtered): 256,660 bp (22.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3264 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.2% C: 0.2% G: 0.2% T: 99.4% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "T" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 4 16.3 0 4 9 1 0.0 0 0 1 10 2 0.0 1 0 2 11 2 0.0 1 0 2 52 2 0.0 1 2 250 1 0.0 1 1 265 1 0.0 1 1 266 16 0.0 1 15 1 267 3097 0.0 1 2969 128 268 107 0.0 1 105 2 269 21 0.0 1 21 270 10 0.0 1 10 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.