This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.26 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 75,685 (2.4%) Reads that were too short: 2,770 (0.1%) Reads written (passing filters): 72,915 (2.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 18,890,292 bp (2.0%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 75685 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 422 777.6 0 422 7 14144 777.6 0 14144 8 13782 777.6 0 13782 9 29 777.6 0 29 10 17 777.6 0 17 11 70 777.6 0 70 12 48 777.6 0 48 13 6 777.6 0 6 14 14 777.6 0 14 15 7 777.6 0 7 16 3 777.6 0 3 17 5 777.6 0 5 18 15 777.6 0 15 19 9 777.6 0 9 20 7 777.6 0 7 21 14 777.6 0 14 22 14 777.6 0 14 23 8 777.6 0 8 24 4 777.6 0 4 25 9 777.6 0 9 26 10 777.6 0 10 27 17 777.6 0 17 28 60 777.6 0 60 29 12 777.6 0 12 30 6 777.6 0 6 31 12 777.6 0 12 32 5 777.6 0 5 33 10 777.6 0 10 34 9 777.6 0 9 35 12 777.6 0 12 36 14 777.6 0 14 37 33 777.6 0 33 38 73 777.6 0 73 39 27 777.6 0 27 40 163 777.6 0 163 41 27 777.6 0 27 42 32 777.6 0 32 43 246 777.6 0 246 44 50 777.6 0 50 45 41 777.6 0 41 46 16 777.6 0 16 47 77 777.6 0 77 48 47 777.6 0 47 49 124 777.6 0 124 50 111 777.6 0 111 51 135 777.6 0 135 52 224 777.6 0 224 53 439 777.6 0 439 54 582 777.6 0 582 55 17287 777.6 0 17287 56 5734 777.6 0 5734 57 11032 777.6 0 11032 58 51 777.6 0 51 59 12 777.6 0 12 60 2 777.6 0 2 61 9 777.6 0 9 62 4 777.6 0 4 63 4 777.6 0 4 64 2 777.6 0 2 65 6 777.6 0 6 66 3 777.6 0 3 67 7 777.6 0 7 68 18 777.6 0 18 69 9 777.6 0 9 70 9 777.6 0 9 71 10 777.6 0 10 72 12 777.6 0 12 73 9 777.6 0 9 74 10 777.6 0 10 75 13 777.6 0 13 76 4 777.6 0 4 77 9 777.6 0 9 78 35 777.6 0 35 79 13 777.6 0 13 80 23 777.6 0 23 81 37 777.6 0 37 82 49 777.6 0 49 83 68 777.6 0 68 84 87 777.6 0 87 85 2408 777.6 0 2408 86 1088 777.6 0 1088 87 1037 777.6 0 1037 88 16 777.6 0 16 89 15 777.6 0 15 90 3 777.6 0 3 91 5 777.6 0 5 92 8 777.6 0 8 93 23 777.6 0 23 94 6 777.6 0 6 95 3 777.6 0 3 96 6 777.6 0 6 97 6 777.6 0 6 98 9 777.6 0 9 99 8 777.6 0 8 100 12 777.6 0 12 101 4 777.6 0 4 102 75 777.6 0 75 103 14 777.6 0 14 104 20 777.6 0 20 105 6 777.6 0 6 106 7 777.6 0 7 107 5 777.6 0 5 108 4 777.6 0 4 109 7 777.6 0 7 110 3 777.6 0 3 111 3 777.6 0 3 112 12 777.6 0 12 113 3 777.6 0 3 114 6 777.6 0 6 116 6 777.6 0 6 117 10 777.6 0 10 118 63 777.6 0 63 119 28 777.6 0 28 120 73 777.6 0 73 121 8 777.6 0 8 122 11 777.6 0 11 123 12 777.6 0 12 124 12 777.6 0 12 125 9 777.6 0 9 126 10 777.6 0 10 127 11 777.6 0 11 128 8 777.6 0 8 129 34 777.6 0 34 130 14 777.6 0 14 131 15 777.6 0 15 132 23 777.6 0 23 133 41 777.6 0 41 134 21 777.6 0 21 135 13 777.6 0 13 136 20 777.6 0 20 137 41 777.6 0 41 138 172 777.6 0 172 139 97 777.6 0 97 140 47 777.6 0 47 141 15 777.6 0 15 142 11 777.6 0 11 143 4 777.6 0 4 144 24 777.6 0 24 145 7 777.6 0 7 146 14 777.6 0 14 147 12 777.6 0 12 148 49 777.6 0 49 149 42 777.6 0 42 150 16 777.6 0 16 151 49 777.6 0 49 152 11 777.6 0 11 153 7 777.6 0 7 154 15 777.6 0 15 155 10 777.6 0 10 156 11 777.6 0 11 157 54 777.6 0 54 158 74 777.6 0 74 159 72 777.6 0 72 160 16 777.6 0 16 161 9 777.6 0 9 162 20 777.6 0 20 163 25 777.6 0 25 164 55 777.6 0 55 165 22 777.6 0 22 166 27 777.6 0 27 167 41 777.6 0 41 168 48 777.6 0 48 169 22 777.6 0 22 170 18 777.6 0 18 171 12 777.6 0 12 172 10 777.6 0 10 173 8 777.6 0 8 174 11 777.6 0 11 175 2 777.6 0 2 176 7 777.6 0 7 177 8 777.6 0 8 178 8 777.6 0 8 179 6 777.6 0 6 180 11 777.6 0 11 181 26 777.6 0 26 182 22 777.6 0 22 183 29 777.6 0 29 184 29 777.6 0 29 185 21 777.6 0 21 186 9 777.6 0 9 187 57 777.6 0 57 188 45 777.6 0 45 189 97 777.6 0 97 190 62 777.6 0 62 191 15 777.6 0 15 192 134 777.6 0 134 193 3 777.6 0 3 194 5 777.6 0 5 195 9 777.6 0 9 196 5 777.6 0 5 197 2 777.6 0 2 198 2 777.6 0 2 199 10 777.6 0 10 200 13 777.6 0 13 201 7 777.6 0 7 202 5 777.6 0 5 203 5 777.6 0 5 204 9 777.6 0 9 205 8 777.6 0 8 206 17 777.6 0 17 207 16 777.6 0 16 208 16 777.6 0 16 209 12 777.6 0 12 210 5 777.6 0 5 211 3 777.6 0 3 212 8 777.6 0 8 213 15 777.6 0 15 214 16 777.6 0 16 215 30 777.6 0 30 216 22 777.6 0 22 217 32 777.6 0 32 218 59 777.6 0 59 219 395 777.6 0 395 220 35 777.6 0 35 221 24 777.6 0 24 222 9 777.6 0 9 223 29 777.6 0 29 224 10 777.6 0 10 225 21 777.6 0 21 226 22 777.6 0 22 227 10 777.6 0 10 228 8 777.6 0 8 229 4 777.6 0 4 230 10 777.6 0 10 231 8 777.6 0 8 232 5 777.6 0 5 233 11 777.6 0 11 234 31 777.6 0 31 235 11 777.6 0 11 236 21 777.6 0 21 237 5 777.6 0 5 238 11 777.6 0 11 239 4 777.6 0 4 240 30 777.6 0 30 241 4 777.6 0 4 242 6 777.6 0 6 243 13 777.6 0 13 244 21 777.6 0 21 245 12 777.6 0 12 246 12 777.6 0 12 247 6 777.6 0 6 248 3 777.6 0 3 249 8 777.6 0 8 250 13 777.6 0 13 251 93 777.6 0 93 252 54 777.6 0 54 253 44 777.6 0 44 254 76 777.6 0 76 255 54 777.6 0 54 256 13 777.6 0 13 257 18 777.6 0 18 258 13 777.6 0 13 259 51 777.6 0 51 260 6 777.6 0 6 261 5 777.6 0 5 262 12 777.6 0 12 263 9 777.6 0 9 264 14 777.6 0 14 265 12 777.6 0 12 266 20 777.6 0 20 267 22 777.6 0 22 268 33 777.6 0 33 269 48 777.6 0 48 270 8 777.6 0 8 271 5 777.6 0 5 272 7 777.6 0 7 273 8 777.6 0 8 274 14 777.6 0 14 275 9 777.6 0 9 276 77 777.6 0 77 277 54 777.6 0 54 278 101 777.6 0 101 279 26 777.6 0 26 280 30 777.6 0 30 281 47 777.6 0 47 282 51 777.6 0 51 283 29 777.6 0 29 284 23 777.6 0 23 285 34 777.6 0 34 286 15 777.6 0 15 287 31 777.6 0 31 288 11 777.6 0 11 289 32 777.6 0 32 290 15 777.6 0 15 291 47 777.6 0 47 292 32 777.6 0 32 293 15 777.6 0 15 294 16 777.6 0 16 295 27 777.6 0 27 296 75 777.6 0 75 297 59 777.6 0 59 298 58 777.6 0 58 299 41 777.6 0 41 300 57 777.6 0 57 301 29 777.6 0 29 Finished in 3.75 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 72,915 Reads with adapters: 14,126 (19.4%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 14,098 (19.3%) Total basepairs processed: 18,890,292 bp Total written (filtered): 3,902,584 bp (20.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14126 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 68 0.0 3 0 62 3 3 17 13905 0.0 3 13325 504 63 13 18 60 0.0 3 14 33 10 3 19 38 0.0 3 0 0 26 12 20 7 0.0 3 3 0 1 3 28 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 143 5 0.0 3 0 0 0 5 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.63 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 14,098 Reads with adapters: 995 (7.1%) Reads written (passing filters): 14,098 (100.0%) Total basepairs processed: 3,902,584 bp Total written (filtered): 3,899,582 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 995 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.7% C: 69.8% G: 2.7% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 980 220.3 0 980 4 14 55.1 0 14 6 1 3.4 0 1