This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 103.68 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 132,051 (4.1%) Reads that were too short: 13,929 (0.4%) Reads written (passing filters): 118,122 (3.7%) Total basepairs processed: 958,672,960 bp Total written (filtered): 21,771,349 bp (2.3%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 132051 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 88 777.6 0 88 7 468 777.6 0 468 8 13833 777.6 0 13833 9 31 777.6 0 31 10 10 777.6 0 10 11 8 777.6 0 8 12 10 777.6 0 10 13 13 777.6 0 13 14 11 777.6 0 11 15 13 777.6 0 13 16 7 777.6 0 7 17 8 777.6 0 8 18 27 777.6 0 27 19 13 777.6 0 13 20 22 777.6 0 22 21 16 777.6 0 16 22 11 777.6 0 11 23 11 777.6 0 11 24 12 777.6 0 12 25 12 777.6 0 12 26 7 777.6 0 7 27 13 777.6 0 13 28 13 777.6 0 13 29 9 777.6 0 9 30 14 777.6 0 14 31 12 777.6 0 12 32 11 777.6 0 11 33 12 777.6 0 12 34 8 777.6 0 8 35 11 777.6 0 11 36 11 777.6 0 11 37 18 777.6 0 18 38 15 777.6 0 15 39 11 777.6 0 11 40 13 777.6 0 13 41 15 777.6 0 15 42 10 777.6 0 10 43 10 777.6 0 10 44 13 777.6 0 13 45 11 777.6 0 11 46 18 777.6 0 18 47 10 777.6 0 10 48 25 777.6 0 25 49 13 777.6 0 13 50 71 777.6 0 71 51 48 777.6 0 48 52 11 777.6 0 11 53 26 777.6 0 26 54 41 777.6 0 41 55 25 777.6 0 25 56 73 777.6 0 73 57 16 777.6 0 16 58 35 777.6 0 35 59 19 777.6 0 19 60 61 777.6 0 61 61 27 777.6 0 27 62 54 777.6 0 54 63 56 777.6 0 56 64 124 777.6 0 124 65 168 777.6 0 168 66 293 777.6 0 293 67 628 777.6 0 628 68 124 777.6 0 124 69 185 777.6 0 185 70 443 777.6 0 443 71 215 777.6 0 215 72 155 777.6 0 155 73 315 777.6 0 315 74 243 777.6 0 243 75 420 777.6 0 420 76 209 777.6 0 209 77 323 777.6 0 323 78 350 777.6 0 350 79 896 777.6 0 896 80 14524 777.6 0 14524 81 10127 777.6 0 10127 82 20528 777.6 0 20528 83 141 777.6 0 141 84 23 777.6 0 23 85 13 777.6 0 13 86 12 777.6 0 12 87 16 777.6 0 16 88 14 777.6 0 14 89 7 777.6 0 7 90 32 777.6 0 32 91 29 777.6 0 29 92 93 777.6 0 93 93 78 777.6 0 78 94 31 777.6 0 31 95 60 777.6 0 60 96 25 777.6 0 25 97 154 777.6 0 154 98 20 777.6 0 20 99 23 777.6 0 23 100 32 777.6 0 32 101 34 777.6 0 34 102 7 777.6 0 7 103 14 777.6 0 14 104 24 777.6 0 24 105 24 777.6 0 24 106 17 777.6 0 17 107 21 777.6 0 21 108 22 777.6 0 22 109 9 777.6 0 9 110 19 777.6 0 19 111 24 777.6 0 24 112 11 777.6 0 11 113 29 777.6 0 29 114 12 777.6 0 12 115 10 777.6 0 10 116 45 777.6 0 45 117 68 777.6 0 68 118 55 777.6 0 55 119 161 777.6 0 161 120 93 777.6 0 93 121 127 777.6 0 127 122 41 777.6 0 41 123 41 777.6 0 41 124 39 777.6 0 39 125 270 777.6 0 270 126 30 777.6 0 30 127 170 777.6 0 170 128 68 777.6 0 68 129 374 777.6 0 374 130 28 777.6 0 28 131 55 777.6 0 55 132 25 777.6 0 25 133 23 777.6 0 23 134 29 777.6 0 29 135 17 777.6 0 17 136 21 777.6 0 21 137 35 777.6 0 35 138 33 777.6 0 33 139 38 777.6 0 38 140 21 777.6 0 21 141 29 777.6 0 29 142 36 777.6 0 36 143 32 777.6 0 32 144 23 777.6 0 23 145 82 777.6 0 82 146 83 777.6 0 83 147 35 777.6 0 35 148 257 777.6 0 257 149 71 777.6 0 71 150 240 777.6 0 240 151 14 777.6 0 14 152 21 777.6 0 21 153 37 777.6 0 37 154 21 777.6 0 21 155 26 777.6 0 26 156 19 777.6 0 19 157 27 777.6 0 27 158 17 777.6 0 17 159 34 777.6 0 34 160 25 777.6 0 25 161 29 777.6 0 29 162 36 777.6 0 36 163 42 777.6 0 42 164 66 777.6 0 66 165 37 777.6 0 37 166 40 777.6 0 40 167 75 777.6 0 75 168 70 777.6 0 70 169 50 777.6 0 50 170 76 777.6 0 76 171 138 777.6 0 138 172 85 777.6 0 85 173 105 777.6 0 105 174 221 777.6 0 221 175 372 777.6 0 372 176 117 777.6 0 117 177 173 777.6 0 173 178 225 777.6 0 225 179 168 777.6 0 168 180 181 777.6 0 181 181 267 777.6 0 267 182 221 777.6 0 221 183 302 777.6 0 302 184 201 777.6 0 201 185 288 777.6 0 288 186 600 777.6 0 600 187 6051 777.6 0 6051 188 20343 777.6 0 20343 189 4814 777.6 0 4814 190 8472 777.6 0 8472 191 582 777.6 0 582 192 306 777.6 0 306 193 620 777.6 0 620 194 426 777.6 0 426 195 1494 777.6 0 1494 196 517 777.6 0 517 197 25 777.6 0 25 198 35 777.6 0 35 199 38 777.6 0 38 200 147 777.6 0 147 201 191 777.6 0 191 202 22 777.6 0 22 203 33 777.6 0 33 204 87 777.6 0 87 205 88 777.6 0 88 206 813 777.6 0 813 207 440 777.6 0 440 208 974 777.6 0 974 209 36 777.6 0 36 210 47 777.6 0 47 211 138 777.6 0 138 212 231 777.6 0 231 213 420 777.6 0 420 214 69 777.6 0 69 215 31 777.6 0 31 216 28 777.6 0 28 217 26 777.6 0 26 218 29 777.6 0 29 219 25 777.6 0 25 220 23 777.6 0 23 221 148 777.6 0 148 222 22 777.6 0 22 223 93 777.6 0 93 224 51 777.6 0 51 225 43 777.6 0 43 226 39 777.6 0 39 227 33 777.6 0 33 228 47 777.6 0 47 229 57 777.6 0 57 230 37 777.6 0 37 231 20 777.6 0 20 232 63 777.6 0 63 233 35 777.6 0 35 234 24 777.6 0 24 235 40 777.6 0 40 236 35 777.6 0 35 237 42 777.6 0 42 238 26 777.6 0 26 239 35 777.6 0 35 240 52 777.6 0 52 241 143 777.6 0 143 242 60 777.6 0 60 243 18 777.6 0 18 244 28 777.6 0 28 245 36 777.6 0 36 246 17 777.6 0 17 247 19 777.6 0 19 248 82 777.6 0 82 249 27 777.6 0 27 250 36 777.6 0 36 251 60 777.6 0 60 252 60 777.6 0 60 253 52 777.6 0 52 254 24 777.6 0 24 255 50 777.6 0 50 256 44 777.6 0 44 257 66 777.6 0 66 258 48 777.6 0 48 259 28 777.6 0 28 260 27 777.6 0 27 261 46 777.6 0 46 262 46 777.6 0 46 263 52 777.6 0 52 264 51 777.6 0 51 265 46 777.6 0 46 266 39 777.6 0 39 267 33 777.6 0 33 268 114 777.6 0 114 269 111 777.6 0 111 270 63 777.6 0 63 271 608 777.6 0 608 272 1713 777.6 0 1713 273 1152 777.6 0 1152 274 275 777.6 0 275 275 66 777.6 0 66 276 45 777.6 0 45 277 43 777.6 0 43 278 21 777.6 0 21 279 17 777.6 0 17 280 30 777.6 0 30 281 94 777.6 0 94 282 274 777.6 0 274 283 132 777.6 0 132 284 266 777.6 0 266 285 22 777.6 0 22 286 21 777.6 0 21 287 26 777.6 0 26 288 48 777.6 0 48 289 55 777.6 0 55 290 70 777.6 0 70 291 255 777.6 0 255 292 638 777.6 0 638 293 565 777.6 0 565 294 786 777.6 0 786 295 124 777.6 0 124 296 52 777.6 0 52 297 73 777.6 0 73 298 178 777.6 0 178 299 115 777.6 0 115 300 99 777.6 0 99 301 38 777.6 0 38 Finished in 5.59 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 118,122 Reads with adapters: 14,261 (12.1%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 14,248 (12.1%) Total basepairs processed: 21,771,349 bp Total written (filtered): 3,885,585 bp (17.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14261 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 12 0.0 3 0 0 0 1 11 18 4 0.0 3 0 0 4 19 125 0.0 3 0 123 2 20 14022 0.0 4 13813 199 7 2 1 21 43 0.0 4 6 34 0 3 22 3 0.0 4 1 0 2 23 8 0.0 4 1 0 0 7 27 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 39 2 0.0 4 0 0 0 0 2 55 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 73 24 0.0 4 0 0 0 0 24 80 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 155 6 0.0 4 0 0 0 0 6 162 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.59 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 14,248 Reads with adapters: 108 (0.8%) Reads written (passing filters): 14,248 (100.0%) Total basepairs processed: 3,885,585 bp Total written (filtered): 3,884,155 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 108 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 99.1% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 55.7 0 1 5 1 13.9 0 1 10 3 0.0 1 0 3 11 102 0.0 1 0 102 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.