This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.39 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 473,805 (14.9%) Reads that were too short: 44,795 (1.4%) Reads written (passing filters): 429,010 (13.5%) Total basepairs processed: 958,672,960 bp Total written (filtered): 93,628,818 bp (9.8%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 473805 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1364 777.6 0 1364 7 59425 777.6 0 59425 8 123 777.6 0 123 9 2357 777.6 0 2357 10 27 777.6 0 27 11 27 777.6 0 27 12 17 777.6 0 17 13 21 777.6 0 21 14 30 777.6 0 30 15 21 777.6 0 21 16 35 777.6 0 35 17 26 777.6 0 26 18 14 777.6 0 14 19 49 777.6 0 49 20 50 777.6 0 50 21 31 777.6 0 31 22 42 777.6 0 42 23 34 777.6 0 34 24 27 777.6 0 27 25 25 777.6 0 25 26 24 777.6 0 24 27 25 777.6 0 25 28 27 777.6 0 27 29 21 777.6 0 21 30 20 777.6 0 20 31 23 777.6 0 23 32 29 777.6 0 29 33 32 777.6 0 32 34 27 777.6 0 27 35 40 777.6 0 40 36 51 777.6 0 51 37 20 777.6 0 20 38 40 777.6 0 40 39 22 777.6 0 22 40 39 777.6 0 39 41 62 777.6 0 62 42 64 777.6 0 64 43 65 777.6 0 65 44 59 777.6 0 59 45 65 777.6 0 65 46 48 777.6 0 48 47 84 777.6 0 84 48 44 777.6 0 44 49 81 777.6 0 81 50 297 777.6 0 297 51 277 777.6 0 277 52 354 777.6 0 354 53 61 777.6 0 61 54 39 777.6 0 39 55 55 777.6 0 55 56 41 777.6 0 41 57 36 777.6 0 36 58 75 777.6 0 75 59 227 777.6 0 227 60 697 777.6 0 697 61 643 777.6 0 643 62 395 777.6 0 395 63 101 777.6 0 101 64 70 777.6 0 70 65 265 777.6 0 265 66 218 777.6 0 218 67 346 777.6 0 346 68 574 777.6 0 574 69 931 777.6 0 931 70 1049 777.6 0 1049 71 2003 777.6 0 2003 72 4088 777.6 0 4088 73 945 777.6 0 945 74 913 777.6 0 913 75 2098 777.6 0 2098 76 1158 777.6 0 1158 77 873 777.6 0 873 78 1905 777.6 0 1905 79 1295 777.6 0 1295 80 2808 777.6 0 2808 81 1327 777.6 0 1327 82 2491 777.6 0 2491 83 2857 777.6 0 2857 84 4111 777.6 0 4111 85 72611 777.6 0 72611 86 90907 777.6 0 90907 87 117564 777.6 0 117564 88 475 777.6 0 475 89 25 777.6 0 25 90 21 777.6 0 21 91 14 777.6 0 14 92 30 777.6 0 30 93 34 777.6 0 34 94 16 777.6 0 16 95 16 777.6 0 16 96 13 777.6 0 13 97 8 777.6 0 8 98 10 777.6 0 10 99 18 777.6 0 18 100 6 777.6 0 6 101 18 777.6 0 18 102 8 777.6 0 8 103 8 777.6 0 8 104 11 777.6 0 11 105 10 777.6 0 10 106 12 777.6 0 12 107 9 777.6 0 9 108 6 777.6 0 6 109 8 777.6 0 8 110 5 777.6 0 5 111 10 777.6 0 10 112 16 777.6 0 16 113 9 777.6 0 9 114 11 777.6 0 11 115 7 777.6 0 7 116 15 777.6 0 15 117 13 777.6 0 13 118 11 777.6 0 11 119 24 777.6 0 24 120 13 777.6 0 13 121 18 777.6 0 18 122 122 777.6 0 122 123 27 777.6 0 27 124 31 777.6 0 31 125 21 777.6 0 21 126 14 777.6 0 14 127 13 777.6 0 13 128 15 777.6 0 15 129 36 777.6 0 36 130 130 777.6 0 130 131 31 777.6 0 31 132 47 777.6 0 47 133 31 777.6 0 31 134 33 777.6 0 33 135 79 777.6 0 79 136 58 777.6 0 58 137 48 777.6 0 48 138 33 777.6 0 33 139 49 777.6 0 49 140 45 777.6 0 45 141 33 777.6 0 33 142 22 777.6 0 22 143 41 777.6 0 41 144 48 777.6 0 48 145 35 777.6 0 35 146 52 777.6 0 52 147 46 777.6 0 46 148 84 777.6 0 84 149 682 777.6 0 682 150 680 777.6 0 680 151 387 777.6 0 387 152 637 777.6 0 637 153 616 777.6 0 616 154 652 777.6 0 652 155 552 777.6 0 552 156 119 777.6 0 119 157 78 777.6 0 78 158 94 777.6 0 94 159 106 777.6 0 106 160 147 777.6 0 147 161 250 777.6 0 250 162 463 777.6 0 463 163 125 777.6 0 125 164 159 777.6 0 159 165 310 777.6 0 310 166 177 777.6 0 177 167 342 777.6 0 342 168 334 777.6 0 334 169 237 777.6 0 237 170 457 777.6 0 457 171 242 777.6 0 242 172 388 777.6 0 388 173 879 777.6 0 879 174 1082 777.6 0 1082 175 12366 777.6 0 12366 176 8109 777.6 0 8109 177 13891 777.6 0 13891 178 67 777.6 0 67 179 23 777.6 0 23 180 23 777.6 0 23 181 80 777.6 0 80 182 36 777.6 0 36 183 55 777.6 0 55 184 27 777.6 0 27 185 19 777.6 0 19 186 56 777.6 0 56 187 18 777.6 0 18 188 9 777.6 0 9 189 23 777.6 0 23 190 45 777.6 0 45 191 41 777.6 0 41 192 21 777.6 0 21 193 31 777.6 0 31 194 16 777.6 0 16 195 21 777.6 0 21 196 55 777.6 0 55 197 33 777.6 0 33 198 31 777.6 0 31 199 23 777.6 0 23 200 34 777.6 0 34 201 48 777.6 0 48 202 35 777.6 0 35 203 61 777.6 0 61 204 90 777.6 0 90 205 95 777.6 0 95 206 1156 777.6 0 1156 207 55 777.6 0 55 208 89 777.6 0 89 209 97 777.6 0 97 210 220 777.6 0 220 211 504 777.6 0 504 212 698 777.6 0 698 213 18485 777.6 0 18485 214 4237 777.6 0 4237 215 10144 777.6 0 10144 216 63 777.6 0 63 217 56 777.6 0 56 218 38 777.6 0 38 219 20 777.6 0 20 220 29 777.6 0 29 221 23 777.6 0 23 222 30 777.6 0 30 223 48 777.6 0 48 224 26 777.6 0 26 225 22 777.6 0 22 226 29 777.6 0 29 227 24 777.6 0 24 228 49 777.6 0 49 229 35 777.6 0 35 230 58 777.6 0 58 231 322 777.6 0 322 232 211 777.6 0 211 233 423 777.6 0 423 234 99 777.6 0 99 235 86 777.6 0 86 236 19 777.6 0 19 237 28 777.6 0 28 238 33 777.6 0 33 239 19 777.6 0 19 240 24 777.6 0 24 241 54 777.6 0 54 242 55 777.6 0 55 243 103 777.6 0 103 244 56 777.6 0 56 245 30 777.6 0 30 246 23 777.6 0 23 247 13 777.6 0 13 248 23 777.6 0 23 249 49 777.6 0 49 250 111 777.6 0 111 251 211 777.6 0 211 252 203 777.6 0 203 253 937 777.6 0 937 254 540 777.6 0 540 255 664 777.6 0 664 256 376 777.6 0 376 257 118 777.6 0 118 258 392 777.6 0 392 259 67 777.6 0 67 260 17 777.6 0 17 261 21 777.6 0 21 262 14 777.6 0 14 263 29 777.6 0 29 264 11 777.6 0 11 265 18 777.6 0 18 266 20 777.6 0 20 267 10 777.6 0 10 268 12 777.6 0 12 269 10 777.6 0 10 270 31 777.6 0 31 271 30 777.6 0 30 272 19 777.6 0 19 273 10 777.6 0 10 274 10 777.6 0 10 275 12 777.6 0 12 276 31 777.6 0 31 277 38 777.6 0 38 278 342 777.6 0 342 279 53 777.6 0 53 280 18 777.6 0 18 281 19 777.6 0 19 282 55 777.6 0 55 283 54 777.6 0 54 284 24 777.6 0 24 285 89 777.6 0 89 286 45 777.6 0 45 287 50 777.6 0 50 288 19 777.6 0 19 289 30 777.6 0 30 290 34 777.6 0 34 291 62 777.6 0 62 292 61 777.6 0 61 293 74 777.6 0 74 294 45 777.6 0 45 295 44 777.6 0 44 296 94 777.6 0 94 297 405 777.6 0 405 298 644 777.6 0 644 299 341 777.6 0 341 300 32 777.6 0 32 301 13 777.6 0 13 Finished in 19.46 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 429,010 Reads with adapters: 6,422 (1.5%) Reads that were too short: 1,117 (0.3%) Reads written (passing filters): 5,305 (1.2%) Total basepairs processed: 93,628,818 bp Total written (filtered): 1,088,698 bp (1.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6422 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 0 2 16 10 0.0 3 0 10 17 2510 0.0 3 2236 104 15 155 18 13 0.0 3 0 7 5 1 19 384 0.0 3 368 6 3 7 20 67 0.0 3 1 0 0 66 21 3 0.0 3 0 0 0 3 22 2 0.0 3 1 0 0 1 36 2 0.0 3 0 0 0 2 37 134 0.0 3 0 0 1 133 38 12 0.0 3 0 0 0 12 41 10 0.0 3 0 0 0 10 50 2 0.0 3 0 0 0 2 51 6 0.0 3 0 0 0 6 53 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 68 5 0.0 3 0 0 0 5 77 1 0.0 3 0 0 0 1 81 12 0.0 3 0 0 0 12 82 4 0.0 3 0 0 0 4 83 3 0.0 3 0 0 0 3 84 2 0.0 3 0 0 0 2 85 4 0.0 3 0 0 0 4 86 1196 0.0 3 0 0 4 1192 87 128 0.0 3 0 0 1 127 88 96 0.0 3 0 0 2 94 89 164 0.0 3 0 0 3 161 90 56 0.0 3 0 0 0 56 91 304 0.0 3 0 0 0 304 92 30 0.0 3 0 0 0 30 94 13 0.0 3 0 0 1 12 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 4 0.0 3 0 0 0 4 99 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 101 9 0.0 3 0 0 0 9 102 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 11 0.0 3 0 0 0 11 107 14 0.0 3 0 0 0 14 109 4 0.0 3 0 0 0 4 110 6 0.0 3 0 0 1 5 111 1 0.0 3 0 0 0 1 112 14 0.0 3 0 0 0 14 113 5 0.0 3 0 0 0 5 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 29 0.0 3 0 0 0 29 132 1 0.0 3 0 0 0 1 134 8 0.0 3 0 0 0 8 137 7 0.0 3 0 0 0 7 138 5 0.0 3 0 0 0 5 139 3 0.0 3 0 0 0 3 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 188 4 0.0 3 0 0 0 4 189 8 0.0 3 0 0 0 8 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 196 0.0 3 0 0 4 192 199 127 0.0 3 0 0 1 126 200 58 0.0 3 0 0 0 58 201 153 0.0 3 0 0 4 149 202 189 0.0 3 0 0 3 186 203 53 0.0 3 0 0 2 51 204 14 0.0 3 0 0 1 13 205 8 0.0 3 0 0 0 8 206 21 0.0 3 0 0 0 21 207 72 0.0 3 0 0 3 69 208 52 0.0 3 0 0 3 49 209 2 0.0 3 0 0 0 2 210 5 0.0 3 0 0 1 4 211 15 0.0 3 0 0 1 14 212 1 0.0 3 0 0 0 1 213 11 0.0 3 0 0 1 10 214 6 0.0 3 0 0 0 6 215 3 0.0 3 0 0 0 3 216 6 0.0 3 0 0 0 6 217 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 225 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 12 0.0 3 0 0 0 12 282 22 0.0 3 0 0 0 22 283 7 0.0 3 0 0 0 7 287 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 289 3 0.0 3 0 0 0 3 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (40 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 5,305 Reads with adapters: 391 (7.4%) Reads written (passing filters): 5,305 (100.0%) Total basepairs processed: 1,088,698 bp Total written (filtered): 1,087,500 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 391 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.1% C: 22.5% G: 24.0% T: 16.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 366 82.9 0 366 4 25 20.7 0 25