This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 105.05 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 238,602 (7.5%) Reads that were too short: 105,781 (3.3%) Reads written (passing filters): 132,821 (4.2%) Total basepairs processed: 958,672,960 bp Total written (filtered): 21,265,604 bp (2.2%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 238602 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 103 777.6 0 103 7 2309 777.6 0 2309 8 21 777.6 0 21 9 13 777.6 0 13 10 22 777.6 0 22 11 22 777.6 0 22 12 11 777.6 0 11 13 14 777.6 0 14 14 13 777.6 0 13 15 11 777.6 0 11 16 8 777.6 0 8 17 9 777.6 0 9 18 15 777.6 0 15 19 19 777.6 0 19 20 8 777.6 0 8 21 14 777.6 0 14 22 16 777.6 0 16 23 16 777.6 0 16 24 14 777.6 0 14 25 17 777.6 0 17 26 24 777.6 0 24 27 17 777.6 0 17 28 22 777.6 0 22 29 21 777.6 0 21 30 14 777.6 0 14 31 19 777.6 0 19 32 12 777.6 0 12 33 14 777.6 0 14 34 22 777.6 0 22 35 12 777.6 0 12 36 20 777.6 0 20 37 14 777.6 0 14 38 23 777.6 0 23 39 12 777.6 0 12 40 17 777.6 0 17 41 15 777.6 0 15 42 23 777.6 0 23 43 26 777.6 0 26 44 20 777.6 0 20 45 13 777.6 0 13 46 24 777.6 0 24 47 20 777.6 0 20 48 15 777.6 0 15 49 18 777.6 0 18 50 18 777.6 0 18 51 15 777.6 0 15 52 13 777.6 0 13 53 14 777.6 0 14 54 15 777.6 0 15 55 22 777.6 0 22 56 30 777.6 0 30 57 17 777.6 0 17 58 27 777.6 0 27 59 13 777.6 0 13 60 68 777.6 0 68 61 17 777.6 0 17 62 25 777.6 0 25 63 18 777.6 0 18 64 15 777.6 0 15 65 22 777.6 0 22 66 17 777.6 0 17 67 24 777.6 0 24 68 23 777.6 0 23 69 15 777.6 0 15 70 30 777.6 0 30 71 19 777.6 0 19 72 17 777.6 0 17 73 20 777.6 0 20 74 131 777.6 0 131 75 33 777.6 0 33 76 43 777.6 0 43 77 38 777.6 0 38 78 59 777.6 0 59 79 74 777.6 0 74 80 103 777.6 0 103 81 235 777.6 0 235 82 234 777.6 0 234 83 321 777.6 0 321 84 338 777.6 0 338 85 295 777.6 0 295 86 252 777.6 0 252 87 577 777.6 0 577 88 817 777.6 0 817 89 162 777.6 0 162 90 728 777.6 0 728 91 593 777.6 0 593 92 92 777.6 0 92 93 612 777.6 0 612 94 1295 777.6 0 1295 95 2264 777.6 0 2264 96 383 777.6 0 383 97 187 777.6 0 187 98 303 777.6 0 303 99 163 777.6 0 163 100 163 777.6 0 163 101 447 777.6 0 447 102 368 777.6 0 368 103 242 777.6 0 242 104 672 777.6 0 672 105 570 777.6 0 570 106 304 777.6 0 304 107 3890 777.6 0 3890 108 696 777.6 0 696 109 403 777.6 0 403 110 313 777.6 0 313 111 355 777.6 0 355 112 282 777.6 0 282 113 294 777.6 0 294 114 442 777.6 0 442 115 178 777.6 0 178 116 320 777.6 0 320 117 249 777.6 0 249 118 225 777.6 0 225 119 567 777.6 0 567 120 999 777.6 0 999 121 849 777.6 0 849 122 592 777.6 0 592 123 494 777.6 0 494 124 499 777.6 0 499 125 614 777.6 0 614 126 816 777.6 0 816 127 755 777.6 0 755 128 854 777.6 0 854 129 752 777.6 0 752 130 377 777.6 0 377 131 502 777.6 0 502 132 586 777.6 0 586 133 2384 777.6 0 2384 134 9370 777.6 0 9370 135 7418 777.6 0 7418 136 9529 777.6 0 9529 137 991 777.6 0 991 138 411 777.6 0 411 139 595 777.6 0 595 140 1144 777.6 0 1144 141 631 777.6 0 631 142 613 777.6 0 613 143 350 777.6 0 350 144 429 777.6 0 429 145 1199 777.6 0 1199 146 1000 777.6 0 1000 147 1482 777.6 0 1482 148 2919 777.6 0 2919 149 720 777.6 0 720 150 551 777.6 0 551 151 443 777.6 0 443 152 1162 777.6 0 1162 153 699 777.6 0 699 154 1320 777.6 0 1320 155 1762 777.6 0 1762 156 2491 777.6 0 2491 157 4966 777.6 0 4966 158 13234 777.6 0 13234 159 2589 777.6 0 2589 160 1128 777.6 0 1128 161 713 777.6 0 713 162 641 777.6 0 641 163 471 777.6 0 471 164 542 777.6 0 542 165 920 777.6 0 920 166 861 777.6 0 861 167 671 777.6 0 671 168 389 777.6 0 389 169 493 777.6 0 493 170 282 777.6 0 282 171 503 777.6 0 503 172 481 777.6 0 481 173 1545 777.6 0 1545 174 1714 777.6 0 1714 175 1068 777.6 0 1068 176 503 777.6 0 503 177 204 777.6 0 204 178 212 777.6 0 212 179 310 777.6 0 310 180 864 777.6 0 864 181 1069 777.6 0 1069 182 826 777.6 0 826 183 487 777.6 0 487 184 437 777.6 0 437 185 814 777.6 0 814 186 1327 777.6 0 1327 187 1017 777.6 0 1017 188 537 777.6 0 537 189 314 777.6 0 314 190 539 777.6 0 539 191 1259 777.6 0 1259 192 1075 777.6 0 1075 193 902 777.6 0 902 194 383 777.6 0 383 195 368 777.6 0 368 196 244 777.6 0 244 197 241 777.6 0 241 198 559 777.6 0 559 199 570 777.6 0 570 200 488 777.6 0 488 201 821 777.6 0 821 202 534 777.6 0 534 203 845 777.6 0 845 204 718 777.6 0 718 205 634 777.6 0 634 206 489 777.6 0 489 207 692 777.6 0 692 208 516 777.6 0 516 209 326 777.6 0 326 210 448 777.6 0 448 211 348 777.6 0 348 212 547 777.6 0 547 213 650 777.6 0 650 214 690 777.6 0 690 215 660 777.6 0 660 216 618 777.6 0 618 217 553 777.6 0 553 218 773 777.6 0 773 219 820 777.6 0 820 220 784 777.6 0 784 221 986 777.6 0 986 222 391 777.6 0 391 223 250 777.6 0 250 224 562 777.6 0 562 225 542 777.6 0 542 226 652 777.6 0 652 227 733 777.6 0 733 228 768 777.6 0 768 229 702 777.6 0 702 230 620 777.6 0 620 231 470 777.6 0 470 232 703 777.6 0 703 233 547 777.6 0 547 234 418 777.6 0 418 235 563 777.6 0 563 236 666 777.6 0 666 237 523 777.6 0 523 238 397 777.6 0 397 239 473 777.6 0 473 240 556 777.6 0 556 241 590 777.6 0 590 242 519 777.6 0 519 243 415 777.6 0 415 244 549 777.6 0 549 245 783 777.6 0 783 246 814 777.6 0 814 247 611 777.6 0 611 248 490 777.6 0 490 249 228 777.6 0 228 250 262 777.6 0 262 251 466 777.6 0 466 252 1176 777.6 0 1176 253 1230 777.6 0 1230 254 861 777.6 0 861 255 902 777.6 0 902 256 1259 777.6 0 1259 257 1066 777.6 0 1066 258 795 777.6 0 795 259 544 777.6 0 544 260 640 777.6 0 640 261 573 777.6 0 573 262 494 777.6 0 494 263 499 777.6 0 499 264 526 777.6 0 526 265 490 777.6 0 490 266 993 777.6 0 993 267 853 777.6 0 853 268 1302 777.6 0 1302 269 849 777.6 0 849 270 810 777.6 0 810 271 891 777.6 0 891 272 749 777.6 0 749 273 667 777.6 0 667 274 814 777.6 0 814 275 1032 777.6 0 1032 276 3961 777.6 0 3961 277 2821 777.6 0 2821 278 2060 777.6 0 2060 279 1615 777.6 0 1615 280 1188 777.6 0 1188 281 1112 777.6 0 1112 282 892 777.6 0 892 283 455 777.6 0 455 284 880 777.6 0 880 285 1165 777.6 0 1165 286 1158 777.6 0 1158 287 1023 777.6 0 1023 288 1035 777.6 0 1035 289 745 777.6 0 745 290 674 777.6 0 674 291 412 777.6 0 412 292 975 777.6 0 975 293 961 777.6 0 961 294 2015 777.6 0 2015 295 12273 777.6 0 12273 296 6754 777.6 0 6754 297 10606 777.6 0 10606 298 317 777.6 0 317 299 286 777.6 0 286 300 452 777.6 0 452 301 1037 777.6 0 1037 Finished in 5.71 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 132,821 Reads with adapters: 2,339 (1.8%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 2,322 (1.7%) Total basepairs processed: 21,265,604 bp Total written (filtered): 635,339 bp (3.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2339 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 9 0.0 3 0 8 0 0 1 20 2300 0.0 4 2093 201 6 21 2 0.0 4 1 1 23 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 45 6 0.0 4 0 0 0 0 6 55 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 102 3 0.0 4 0 0 0 0 3 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 2,322 Reads with adapters: 6 (0.3%) Reads written (passing filters): 2,322 (100.0%) Total basepairs processed: 635,339 bp Total written (filtered): 634,959 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 50.0% C: 0.0% G: 33.3% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 36.3 0 2 11 1 0.0 1 0 1 13 1 0.0 1 1 140 1 0.0 1 1 210 1 0.0 1 0 1