This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.41 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 69,288 (2.2%) Reads that were too short: 4,810 (0.2%) Reads written (passing filters): 64,478 (2.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 18,145,828 bp (1.9%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 69288 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4675 777.6 0 4675 7 1005 777.6 0 1005 8 9831 777.6 0 9831 9 41983 777.6 0 41983 10 51 777.6 0 51 11 11 777.6 0 11 12 10 777.6 0 10 13 3 777.6 0 3 14 3 777.6 0 3 15 12 777.6 0 12 16 2 777.6 0 2 17 3 777.6 0 3 18 3 777.6 0 3 19 10 777.6 0 10 20 10 777.6 0 10 21 9 777.6 0 9 22 7 777.6 0 7 23 6 777.6 0 6 24 5 777.6 0 5 25 10 777.6 0 10 26 3 777.6 0 3 27 7 777.6 0 7 28 2 777.6 0 2 29 10 777.6 0 10 30 4 777.6 0 4 31 4 777.6 0 4 32 3 777.6 0 3 33 50 777.6 0 50 34 10 777.6 0 10 35 11 777.6 0 11 36 98 777.6 0 98 37 12 777.6 0 12 38 27 777.6 0 27 39 12 777.6 0 12 40 18 777.6 0 18 41 5 777.6 0 5 42 12 777.6 0 12 43 20 777.6 0 20 44 93 777.6 0 93 45 115 777.6 0 115 46 3 777.6 0 3 47 4 777.6 0 4 48 11 777.6 0 11 49 8 777.6 0 8 50 8 777.6 0 8 51 9 777.6 0 9 52 13 777.6 0 13 53 7 777.6 0 7 54 8 777.6 0 8 55 5 777.6 0 5 56 9 777.6 0 9 57 17 777.6 0 17 58 162 777.6 0 162 59 47 777.6 0 47 60 97 777.6 0 97 61 10 777.6 0 10 62 16 777.6 0 16 63 37 777.6 0 37 64 7 777.6 0 7 65 2 777.6 0 2 66 6 777.6 0 6 67 5 777.6 0 5 68 3 777.6 0 3 69 8 777.6 0 8 70 27 777.6 0 27 71 11 777.6 0 11 72 9 777.6 0 9 73 10 777.6 0 10 74 9 777.6 0 9 75 19 777.6 0 19 76 470 777.6 0 470 77 194 777.6 0 194 78 217 777.6 0 217 79 16 777.6 0 16 80 331 777.6 0 331 81 81 777.6 0 81 82 75 777.6 0 75 83 18 777.6 0 18 84 11 777.6 0 11 85 10 777.6 0 10 86 11 777.6 0 11 87 79 777.6 0 79 88 22 777.6 0 22 89 8 777.6 0 8 90 25 777.6 0 25 91 34 777.6 0 34 92 11 777.6 0 11 93 5 777.6 0 5 94 12 777.6 0 12 95 18 777.6 0 18 96 31 777.6 0 31 97 76 777.6 0 76 98 109 777.6 0 109 99 26 777.6 0 26 100 107 777.6 0 107 101 218 777.6 0 218 102 254 777.6 0 254 103 12 777.6 0 12 104 38 777.6 0 38 105 16 777.6 0 16 106 10 777.6 0 10 107 12 777.6 0 12 108 17 777.6 0 17 109 14 777.6 0 14 110 164 777.6 0 164 111 111 777.6 0 111 112 408 777.6 0 408 113 13 777.6 0 13 114 3 777.6 0 3 116 17 777.6 0 17 117 16 777.6 0 16 118 32 777.6 0 32 119 17 777.6 0 17 120 14 777.6 0 14 121 15 777.6 0 15 122 20 777.6 0 20 123 9 777.6 0 9 124 10 777.6 0 10 125 7 777.6 0 7 126 6 777.6 0 6 127 18 777.6 0 18 128 13 777.6 0 13 129 18 777.6 0 18 130 12 777.6 0 12 131 16 777.6 0 16 132 22 777.6 0 22 133 19 777.6 0 19 134 17 777.6 0 17 135 19 777.6 0 19 136 17 777.6 0 17 137 19 777.6 0 19 138 22 777.6 0 22 139 19 777.6 0 19 140 17 777.6 0 17 141 14 777.6 0 14 142 34 777.6 0 34 143 20 777.6 0 20 144 19 777.6 0 19 145 7 777.6 0 7 146 12 777.6 0 12 147 26 777.6 0 26 148 14 777.6 0 14 149 16 777.6 0 16 150 10 777.6 0 10 151 21 777.6 0 21 152 24 777.6 0 24 153 8 777.6 0 8 154 14 777.6 0 14 155 19 777.6 0 19 156 16 777.6 0 16 157 78 777.6 0 78 158 18 777.6 0 18 159 28 777.6 0 28 160 61 777.6 0 61 161 30 777.6 0 30 162 42 777.6 0 42 163 37 777.6 0 37 164 22 777.6 0 22 165 30 777.6 0 30 166 32 777.6 0 32 167 43 777.6 0 43 168 221 777.6 0 221 169 67 777.6 0 67 170 33 777.6 0 33 171 31 777.6 0 31 172 9 777.6 0 9 173 5 777.6 0 5 174 7 777.6 0 7 175 18 777.6 0 18 176 17 777.6 0 17 177 23 777.6 0 23 178 21 777.6 0 21 179 13 777.6 0 13 180 15 777.6 0 15 181 21 777.6 0 21 182 45 777.6 0 45 183 34 777.6 0 34 184 44 777.6 0 44 185 27 777.6 0 27 186 29 777.6 0 29 187 35 777.6 0 35 188 30 777.6 0 30 189 33 777.6 0 33 190 13 777.6 0 13 191 13 777.6 0 13 192 18 777.6 0 18 193 28 777.6 0 28 194 27 777.6 0 27 195 42 777.6 0 42 196 39 777.6 0 39 197 25 777.6 0 25 198 36 777.6 0 36 199 86 777.6 0 86 200 136 777.6 0 136 201 92 777.6 0 92 202 38 777.6 0 38 203 44 777.6 0 44 204 159 777.6 0 159 205 55 777.6 0 55 206 52 777.6 0 52 207 102 777.6 0 102 208 36 777.6 0 36 209 25 777.6 0 25 210 12 777.6 0 12 211 20 777.6 0 20 212 36 777.6 0 36 213 42 777.6 0 42 214 74 777.6 0 74 215 69 777.6 0 69 216 57 777.6 0 57 217 44 777.6 0 44 218 61 777.6 0 61 219 52 777.6 0 52 220 43 777.6 0 43 221 98 777.6 0 98 222 98 777.6 0 98 223 104 777.6 0 104 224 137 777.6 0 137 225 63 777.6 0 63 226 51 777.6 0 51 227 42 777.6 0 42 228 62 777.6 0 62 229 57 777.6 0 57 230 37 777.6 0 37 231 23 777.6 0 23 232 33 777.6 0 33 233 33 777.6 0 33 234 14 777.6 0 14 235 16 777.6 0 16 236 39 777.6 0 39 237 46 777.6 0 46 238 39 777.6 0 39 239 40 777.6 0 40 240 39 777.6 0 39 241 52 777.6 0 52 242 35 777.6 0 35 243 30 777.6 0 30 244 36 777.6 0 36 245 34 777.6 0 34 246 22 777.6 0 22 247 15 777.6 0 15 248 43 777.6 0 43 249 72 777.6 0 72 250 40 777.6 0 40 251 30 777.6 0 30 252 22 777.6 0 22 253 59 777.6 0 59 254 41 777.6 0 41 255 50 777.6 0 50 256 27 777.6 0 27 257 52 777.6 0 52 258 77 777.6 0 77 259 54 777.6 0 54 260 53 777.6 0 53 261 23 777.6 0 23 262 19 777.6 0 19 263 25 777.6 0 25 264 25 777.6 0 25 265 31 777.6 0 31 266 21 777.6 0 21 267 33 777.6 0 33 268 75 777.6 0 75 269 87 777.6 0 87 270 44 777.6 0 44 271 17 777.6 0 17 272 34 777.6 0 34 273 21 777.6 0 21 274 25 777.6 0 25 275 47 777.6 0 47 276 38 777.6 0 38 277 39 777.6 0 39 278 23 777.6 0 23 279 23 777.6 0 23 280 33 777.6 0 33 281 67 777.6 0 67 282 46 777.6 0 46 283 43 777.6 0 43 284 20 777.6 0 20 285 17 777.6 0 17 286 51 777.6 0 51 287 48 777.6 0 48 288 49 777.6 0 49 289 155 777.6 0 155 290 62 777.6 0 62 291 70 777.6 0 70 292 33 777.6 0 33 293 37 777.6 0 37 294 114 777.6 0 114 295 106 777.6 0 106 296 108 777.6 0 108 297 30 777.6 0 30 298 48 777.6 0 48 299 45 777.6 0 45 300 25 777.6 0 25 301 17 777.6 0 17 Finished in 3.53 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 64,478 Reads with adapters: 13,720 (21.3%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 13,696 (21.2%) Total basepairs processed: 18,145,828 bp Total written (filtered): 3,782,253 bp (20.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13720 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 55 0.0 3 0 52 2 1 17 9182 0.0 3 8844 308 20 10 18 4365 0.0 3 4187 148 21 9 19 50 0.0 3 2 9 32 7 20 24 0.0 3 0 0 3 21 21 2 0.0 3 0 0 0 2 34 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 1 164 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 280 1 0.0 3 0 0 0 1 282 5 0.0 3 0 0 0 5 283 9 0.0 3 0 0 0 9 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 13,696 Reads with adapters: 596 (4.4%) Reads written (passing filters): 13,696 (100.0%) Total basepairs processed: 3,782,253 bp Total written (filtered): 3,780,385 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 596 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.4% C: 34.1% G: 14.1% T: 16.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 530 214.0 0 530 4 53 53.5 0 53 5 12 13.4 0 12 6 1 3.3 0 1