This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.53 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 421,199 (13.2%) Reads that were too short: 19,088 (0.6%) Reads written (passing filters): 402,111 (12.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 69,539,199 bp (7.3%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 421199 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 402 777.6 0 402 7 32236 777.6 0 32236 8 9808 777.6 0 9808 9 10 777.6 0 10 10 10 777.6 0 10 11 94 777.6 0 94 12 28 777.6 0 28 13 14 777.6 0 14 14 16 777.6 0 16 15 4 777.6 0 4 16 8 777.6 0 8 17 14 777.6 0 14 18 9 777.6 0 9 19 9 777.6 0 9 20 8 777.6 0 8 21 7 777.6 0 7 22 10 777.6 0 10 23 7 777.6 0 7 24 13 777.6 0 13 25 16 777.6 0 16 26 13 777.6 0 13 27 17 777.6 0 17 28 11 777.6 0 11 29 4 777.6 0 4 30 2 777.6 0 2 31 15 777.6 0 15 32 19 777.6 0 19 33 9 777.6 0 9 34 22 777.6 0 22 35 6 777.6 0 6 36 12 777.6 0 12 37 12 777.6 0 12 38 93 777.6 0 93 39 64 777.6 0 64 40 15 777.6 0 15 41 8 777.6 0 8 42 17 777.6 0 17 43 50 777.6 0 50 44 18 777.6 0 18 45 9 777.6 0 9 46 15 777.6 0 15 47 14 777.6 0 14 48 6 777.6 0 6 49 5 777.6 0 5 50 10 777.6 0 10 51 10 777.6 0 10 52 6 777.6 0 6 53 10 777.6 0 10 54 11 777.6 0 11 55 10 777.6 0 10 56 9 777.6 0 9 57 13 777.6 0 13 58 9 777.6 0 9 59 15 777.6 0 15 60 8 777.6 0 8 61 6 777.6 0 6 62 9 777.6 0 9 63 9 777.6 0 9 64 90 777.6 0 90 65 75 777.6 0 75 66 96 777.6 0 96 67 9 777.6 0 9 68 11 777.6 0 11 69 48 777.6 0 48 70 18 777.6 0 18 71 35 777.6 0 35 72 30 777.6 0 30 73 22 777.6 0 22 74 18 777.6 0 18 75 28 777.6 0 28 76 29 777.6 0 29 77 63 777.6 0 63 78 117 777.6 0 117 79 54 777.6 0 54 80 142 777.6 0 142 81 134 777.6 0 134 82 141 777.6 0 141 83 175 777.6 0 175 84 142 777.6 0 142 85 247 777.6 0 247 86 57 777.6 0 57 87 43 777.6 0 43 88 146 777.6 0 146 89 293 777.6 0 293 90 297 777.6 0 297 91 231 777.6 0 231 92 67 777.6 0 67 93 657 777.6 0 657 94 91 777.6 0 91 95 223 777.6 0 223 96 141 777.6 0 141 97 70 777.6 0 70 98 116 777.6 0 116 99 218 777.6 0 218 100 224 777.6 0 224 101 679 777.6 0 679 102 831 777.6 0 831 103 147 777.6 0 147 104 183 777.6 0 183 105 228 777.6 0 228 106 180 777.6 0 180 107 148 777.6 0 148 108 51 777.6 0 51 109 82 777.6 0 82 110 278 777.6 0 278 111 668 777.6 0 668 112 341 777.6 0 341 113 231 777.6 0 231 114 259 777.6 0 259 115 600 777.6 0 600 116 147 777.6 0 147 117 79 777.6 0 79 118 137 777.6 0 137 119 150 777.6 0 150 120 92 777.6 0 92 121 116 777.6 0 116 122 305 777.6 0 305 123 211 777.6 0 211 124 431 777.6 0 431 125 731 777.6 0 731 126 192 777.6 0 192 127 381 777.6 0 381 128 231 777.6 0 231 129 257 777.6 0 257 130 356 777.6 0 356 131 353 777.6 0 353 132 434 777.6 0 434 133 183 777.6 0 183 134 198 777.6 0 198 135 378 777.6 0 378 136 446 777.6 0 446 137 776 777.6 0 776 138 1363 777.6 0 1363 139 1846 777.6 0 1846 140 3939 777.6 0 3939 141 4535 777.6 0 4535 142 123289 777.6 0 123289 143 147864 777.6 0 147864 144 39158 777.6 0 39158 145 399 777.6 0 399 146 227 777.6 0 227 147 371 777.6 0 371 148 247 777.6 0 247 149 317 777.6 0 317 150 131 777.6 0 131 151 106 777.6 0 106 152 131 777.6 0 131 153 116 777.6 0 116 154 94 777.6 0 94 155 403 777.6 0 403 156 192 777.6 0 192 157 367 777.6 0 367 158 316 777.6 0 316 159 177 777.6 0 177 160 251 777.6 0 251 161 325 777.6 0 325 162 6101 777.6 0 6101 163 1351 777.6 0 1351 164 2776 777.6 0 2776 165 126 777.6 0 126 166 126 777.6 0 126 167 308 777.6 0 308 168 243 777.6 0 243 169 359 777.6 0 359 170 104 777.6 0 104 171 85 777.6 0 85 172 44 777.6 0 44 173 90 777.6 0 90 174 97 777.6 0 97 175 179 777.6 0 179 176 118 777.6 0 118 177 182 777.6 0 182 178 223 777.6 0 223 179 180 777.6 0 180 180 172 777.6 0 172 181 94 777.6 0 94 182 110 777.6 0 110 183 137 777.6 0 137 184 259 777.6 0 259 185 252 777.6 0 252 186 163 777.6 0 163 187 145 777.6 0 145 188 201 777.6 0 201 189 300 777.6 0 300 190 471 777.6 0 471 191 185 777.6 0 185 192 109 777.6 0 109 193 177 777.6 0 177 194 112 777.6 0 112 195 84 777.6 0 84 196 87 777.6 0 87 197 140 777.6 0 140 198 356 777.6 0 356 199 207 777.6 0 207 200 55 777.6 0 55 201 37 777.6 0 37 202 57 777.6 0 57 203 226 777.6 0 226 204 470 777.6 0 470 205 330 777.6 0 330 206 198 777.6 0 198 207 144 777.6 0 144 208 96 777.6 0 96 209 191 777.6 0 191 210 258 777.6 0 258 211 188 777.6 0 188 212 189 777.6 0 189 213 165 777.6 0 165 214 318 777.6 0 318 215 353 777.6 0 353 216 251 777.6 0 251 217 279 777.6 0 279 218 252 777.6 0 252 219 167 777.6 0 167 220 68 777.6 0 68 221 42 777.6 0 42 222 87 777.6 0 87 223 305 777.6 0 305 224 600 777.6 0 600 225 170 777.6 0 170 226 162 777.6 0 162 227 244 777.6 0 244 228 135 777.6 0 135 229 159 777.6 0 159 230 161 777.6 0 161 231 80 777.6 0 80 232 73 777.6 0 73 233 122 777.6 0 122 234 353 777.6 0 353 235 265 777.6 0 265 236 166 777.6 0 166 237 159 777.6 0 159 238 96 777.6 0 96 239 104 777.6 0 104 240 82 777.6 0 82 241 59 777.6 0 59 242 76 777.6 0 76 243 157 777.6 0 157 244 280 777.6 0 280 245 344 777.6 0 344 246 211 777.6 0 211 247 131 777.6 0 131 248 83 777.6 0 83 249 57 777.6 0 57 250 69 777.6 0 69 251 80 777.6 0 80 252 119 777.6 0 119 253 266 777.6 0 266 254 234 777.6 0 234 255 186 777.6 0 186 256 150 777.6 0 150 257 143 777.6 0 143 258 276 777.6 0 276 259 110 777.6 0 110 260 109 777.6 0 109 261 145 777.6 0 145 262 135 777.6 0 135 263 171 777.6 0 171 264 189 777.6 0 189 265 236 777.6 0 236 266 405 777.6 0 405 267 205 777.6 0 205 268 734 777.6 0 734 269 218 777.6 0 218 270 126 777.6 0 126 271 91 777.6 0 91 272 136 777.6 0 136 273 157 777.6 0 157 274 136 777.6 0 136 275 95 777.6 0 95 276 100 777.6 0 100 277 87 777.6 0 87 278 133 777.6 0 133 279 330 777.6 0 330 280 184 777.6 0 184 281 124 777.6 0 124 282 92 777.6 0 92 283 100 777.6 0 100 284 173 777.6 0 173 285 167 777.6 0 167 286 183 777.6 0 183 287 113 777.6 0 113 288 118 777.6 0 118 289 206 777.6 0 206 290 139 777.6 0 139 291 151 777.6 0 151 292 461 777.6 0 461 293 265 777.6 0 265 294 227 777.6 0 227 295 238 777.6 0 238 296 153 777.6 0 153 297 142 777.6 0 142 298 294 777.6 0 294 299 174 777.6 0 174 300 354 777.6 0 354 301 296 777.6 0 296 Finished in 15.93 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 402,111 Reads with adapters: 32,417 (8.1%) Reads that were too short: 60 (0.0%) Reads written (passing filters): 32,357 (8.0%) Total basepairs processed: 69,539,199 bp Total written (filtered): 8,962,111 bp (12.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 32417 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 11 0.0 3 0 0 7 4 16 554 0.0 3 0 520 26 8 17 31596 0.0 3 30541 917 108 30 18 93 0.0 3 7 76 5 5 19 49 0.0 3 1 0 30 18 20 39 0.0 3 0 0 1 38 28 1 0.0 3 0 0 0 1 31 1 0.0 3 1 36 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 18 0.0 3 0 0 1 17 148 7 0.0 3 0 0 0 7 150 1 0.0 3 0 0 0 1 153 11 0.0 3 0 0 0 11 154 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.50 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 32,357 Reads with adapters: 3,916 (12.1%) Reads written (passing filters): 32,357 (100.0%) Total basepairs processed: 8,962,111 bp Total written (filtered): 8,950,245 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3916 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.0% C: 60.8% G: 10.4% T: 10.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3806 505.6 0 3806 4 104 126.4 0 104 5 4 31.6 0 4 6 2 7.9 0 2