This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 103.41 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 61,235 (1.9%) Reads that were too short: 13,526 (0.4%) Reads written (passing filters): 47,709 (1.5%) Total basepairs processed: 958,672,960 bp Total written (filtered): 12,541,015 bp (1.3%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 61235 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 288 777.6 0 288 7 1274 777.6 0 1274 8 34871 777.6 0 34871 9 38 777.6 0 38 10 2 777.6 0 2 11 2 777.6 0 2 12 1 777.6 0 1 13 1 777.6 0 1 14 4 777.6 0 4 15 1 777.6 0 1 16 4 777.6 0 4 17 2 777.6 0 2 18 25 777.6 0 25 19 17 777.6 0 17 20 20 777.6 0 20 21 3 777.6 0 3 22 9 777.6 0 9 23 11 777.6 0 11 24 4 777.6 0 4 25 2 777.6 0 2 26 4 777.6 0 4 27 5 777.6 0 5 28 3 777.6 0 3 29 12 777.6 0 12 30 7 777.6 0 7 31 7 777.6 0 7 32 5 777.6 0 5 33 7 777.6 0 7 34 7 777.6 0 7 35 14 777.6 0 14 36 200 777.6 0 200 37 78 777.6 0 78 38 166 777.6 0 166 39 5 777.6 0 5 40 4 777.6 0 4 41 8 777.6 0 8 42 70 777.6 0 70 43 58 777.6 0 58 44 126 777.6 0 126 45 85 777.6 0 85 46 8 777.6 0 8 47 6 777.6 0 6 49 6 777.6 0 6 50 3 777.6 0 3 51 3 777.6 0 3 52 5 777.6 0 5 53 4 777.6 0 4 54 1 777.6 0 1 55 3 777.6 0 3 56 5 777.6 0 5 57 4 777.6 0 4 58 11 777.6 0 11 59 3 777.6 0 3 60 3 777.6 0 3 61 2 777.6 0 2 62 2 777.6 0 2 63 5 777.6 0 5 64 2 777.6 0 2 65 1 777.6 0 1 66 2 777.6 0 2 67 3 777.6 0 3 68 6 777.6 0 6 69 2 777.6 0 2 70 3 777.6 0 3 71 19 777.6 0 19 72 1 777.6 0 1 73 4 777.6 0 4 74 3 777.6 0 3 75 5 777.6 0 5 76 1 777.6 0 1 77 5 777.6 0 5 78 2 777.6 0 2 79 15 777.6 0 15 80 6 777.6 0 6 81 6 777.6 0 6 82 10 777.6 0 10 83 12 777.6 0 12 84 9 777.6 0 9 85 2 777.6 0 2 86 4 777.6 0 4 87 7 777.6 0 7 88 20 777.6 0 20 89 31 777.6 0 31 90 20 777.6 0 20 91 70 777.6 0 70 92 22 777.6 0 22 93 69 777.6 0 69 94 341 777.6 0 341 95 91 777.6 0 91 96 35 777.6 0 35 97 52 777.6 0 52 98 34 777.6 0 34 99 107 777.6 0 107 100 61 777.6 0 61 101 37 777.6 0 37 102 7 777.6 0 7 103 6 777.6 0 6 104 21 777.6 0 21 105 14 777.6 0 14 106 10 777.6 0 10 107 16 777.6 0 16 108 31 777.6 0 31 109 29 777.6 0 29 110 12 777.6 0 12 111 18 777.6 0 18 112 10 777.6 0 10 113 29 777.6 0 29 114 19 777.6 0 19 115 22 777.6 0 22 116 33 777.6 0 33 117 38 777.6 0 38 118 19 777.6 0 19 119 28 777.6 0 28 120 96 777.6 0 96 121 76 777.6 0 76 122 96 777.6 0 96 123 40 777.6 0 40 124 41 777.6 0 41 125 40 777.6 0 40 126 36 777.6 0 36 127 45 777.6 0 45 128 53 777.6 0 53 129 139 777.6 0 139 130 103 777.6 0 103 131 118 777.6 0 118 132 300 777.6 0 300 133 99 777.6 0 99 134 141 777.6 0 141 135 783 777.6 0 783 136 539 777.6 0 539 137 773 777.6 0 773 138 93 777.6 0 93 139 119 777.6 0 119 140 114 777.6 0 114 141 94 777.6 0 94 142 279 777.6 0 279 143 101 777.6 0 101 144 128 777.6 0 128 145 110 777.6 0 110 146 52 777.6 0 52 147 52 777.6 0 52 148 102 777.6 0 102 149 223 777.6 0 223 150 68 777.6 0 68 151 41 777.6 0 41 152 36 777.6 0 36 153 40 777.6 0 40 154 42 777.6 0 42 155 52 777.6 0 52 156 91 777.6 0 91 157 42 777.6 0 42 158 62 777.6 0 62 159 86 777.6 0 86 160 38 777.6 0 38 161 44 777.6 0 44 162 27 777.6 0 27 163 65 777.6 0 65 164 55 777.6 0 55 165 67 777.6 0 67 166 94 777.6 0 94 167 104 777.6 0 104 168 93 777.6 0 93 169 32 777.6 0 32 170 24 777.6 0 24 171 27 777.6 0 27 172 46 777.6 0 46 173 58 777.6 0 58 174 66 777.6 0 66 175 34 777.6 0 34 176 19 777.6 0 19 177 28 777.6 0 28 178 40 777.6 0 40 179 64 777.6 0 64 180 108 777.6 0 108 181 90 777.6 0 90 182 77 777.6 0 77 183 72 777.6 0 72 184 96 777.6 0 96 185 59 777.6 0 59 186 45 777.6 0 45 187 89 777.6 0 89 188 146 777.6 0 146 189 152 777.6 0 152 190 200 777.6 0 200 191 114 777.6 0 114 192 101 777.6 0 101 193 47 777.6 0 47 194 53 777.6 0 53 195 56 777.6 0 56 196 262 777.6 0 262 197 128 777.6 0 128 198 28 777.6 0 28 199 40 777.6 0 40 200 19 777.6 0 19 201 151 777.6 0 151 202 463 777.6 0 463 203 417 777.6 0 417 204 224 777.6 0 224 205 59 777.6 0 59 206 23 777.6 0 23 207 58 777.6 0 58 208 153 777.6 0 153 209 210 777.6 0 210 210 146 777.6 0 146 211 142 777.6 0 142 212 165 777.6 0 165 213 140 777.6 0 140 214 109 777.6 0 109 215 178 777.6 0 178 216 166 777.6 0 166 217 88 777.6 0 88 218 61 777.6 0 61 219 31 777.6 0 31 220 65 777.6 0 65 221 77 777.6 0 77 222 261 777.6 0 261 223 87 777.6 0 87 224 150 777.6 0 150 225 146 777.6 0 146 226 117 777.6 0 117 227 86 777.6 0 86 228 82 777.6 0 82 229 38 777.6 0 38 230 36 777.6 0 36 231 23 777.6 0 23 232 45 777.6 0 45 233 88 777.6 0 88 234 77 777.6 0 77 235 214 777.6 0 214 236 127 777.6 0 127 237 74 777.6 0 74 238 28 777.6 0 28 239 23 777.6 0 23 240 26 777.6 0 26 241 60 777.6 0 60 242 82 777.6 0 82 243 162 777.6 0 162 244 143 777.6 0 143 245 72 777.6 0 72 246 31 777.6 0 31 247 16 777.6 0 16 248 19 777.6 0 19 249 35 777.6 0 35 250 44 777.6 0 44 251 139 777.6 0 139 252 89 777.6 0 89 253 101 777.6 0 101 254 70 777.6 0 70 255 104 777.6 0 104 256 87 777.6 0 87 257 36 777.6 0 36 258 41 777.6 0 41 259 53 777.6 0 53 260 75 777.6 0 75 261 116 777.6 0 116 262 117 777.6 0 117 263 92 777.6 0 92 264 109 777.6 0 109 265 91 777.6 0 91 266 79 777.6 0 79 267 41 777.6 0 41 268 20 777.6 0 20 269 163 777.6 0 163 270 377 777.6 0 377 271 296 777.6 0 296 272 163 777.6 0 163 273 113 777.6 0 113 274 81 777.6 0 81 275 102 777.6 0 102 276 63 777.6 0 63 277 96 777.6 0 96 278 96 777.6 0 96 279 520 777.6 0 520 280 160 777.6 0 160 281 152 777.6 0 152 282 127 777.6 0 127 283 112 777.6 0 112 284 333 777.6 0 333 285 152 777.6 0 152 286 129 777.6 0 129 287 188 777.6 0 188 288 54 777.6 0 54 289 46 777.6 0 46 290 101 777.6 0 101 291 279 777.6 0 279 292 198 777.6 0 198 293 63 777.6 0 63 294 96 777.6 0 96 295 289 777.6 0 289 296 292 777.6 0 292 297 244 777.6 0 244 298 401 777.6 0 401 299 303 777.6 0 303 300 433 777.6 0 433 301 477 777.6 0 477 Finished in 2.38 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 47,709 Reads with adapters: 36,288 (76.1%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 36,287 (76.1%) Total basepairs processed: 12,541,015 bp Total written (filtered): 9,907,744 bp (79.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 36288 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 24 0.0 3 0 0 23 0 1 19 517 0.0 3 0 506 4 2 5 20 35637 0.0 4 35083 531 22 1 21 72 0.0 4 21 49 1 0 1 22 6 0.0 4 2 1 3 23 21 0.0 4 1 0 0 16 4 29 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 0 3 98 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.53 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 36,287 Reads with adapters: 1,073 (3.0%) Reads written (passing filters): 36,287 (100.0%) Total basepairs processed: 9,907,744 bp Total written (filtered): 9,864,701 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1073 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 14.4% G: 84.2% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 567.0 0 3 4 1 141.7 0 1 5 3 35.4 0 3 9 1 0.1 0 0 1 10 61 0.0 1 3 58 11 855 0.0 1 2 853 12 2 0.0 1 0 2 224 142 0.0 1 125 17 225 4 0.0 1 4 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.