This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.41 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 103,134 (3.2%) Reads that were too short: 30,834 (1.0%) Reads written (passing filters): 72,300 (2.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 16,335,614 bp (1.7%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 103134 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 473 777.6 0 473 7 7678 777.6 0 7678 8 359 777.6 0 359 9 26529 777.6 0 26529 10 33 777.6 0 33 11 14 777.6 0 14 12 14 777.6 0 14 13 11 777.6 0 11 14 9 777.6 0 9 15 24 777.6 0 24 16 9 777.6 0 9 17 14 777.6 0 14 18 18 777.6 0 18 19 12 777.6 0 12 20 19 777.6 0 19 21 17 777.6 0 17 22 47 777.6 0 47 23 29 777.6 0 29 24 203 777.6 0 203 25 20 777.6 0 20 26 27 777.6 0 27 27 48 777.6 0 48 28 601 777.6 0 601 29 459 777.6 0 459 30 720 777.6 0 720 31 684 777.6 0 684 32 53 777.6 0 53 33 472 777.6 0 472 34 23 777.6 0 23 35 7 777.6 0 7 36 18 777.6 0 18 37 17 777.6 0 17 38 61 777.6 0 61 39 14 777.6 0 14 40 13 777.6 0 13 41 12 777.6 0 12 42 12 777.6 0 12 43 12 777.6 0 12 44 13 777.6 0 13 45 19 777.6 0 19 46 12 777.6 0 12 47 13 777.6 0 13 48 14 777.6 0 14 49 9 777.6 0 9 50 80 777.6 0 80 51 21 777.6 0 21 52 44 777.6 0 44 53 13 777.6 0 13 54 20 777.6 0 20 55 10 777.6 0 10 56 13 777.6 0 13 57 21 777.6 0 21 58 22 777.6 0 22 59 148 777.6 0 148 60 6 777.6 0 6 61 9 777.6 0 9 62 20 777.6 0 20 63 14 777.6 0 14 64 18 777.6 0 18 65 19 777.6 0 19 66 21 777.6 0 21 67 60 777.6 0 60 68 382 777.6 0 382 69 299 777.6 0 299 70 160 777.6 0 160 71 9 777.6 0 9 72 13 777.6 0 13 73 26 777.6 0 26 74 41 777.6 0 41 75 36 777.6 0 36 76 19 777.6 0 19 77 33 777.6 0 33 78 86 777.6 0 86 79 245 777.6 0 245 80 188 777.6 0 188 81 191 777.6 0 191 82 85 777.6 0 85 83 94 777.6 0 94 84 156 777.6 0 156 85 438 777.6 0 438 86 558 777.6 0 558 87 134 777.6 0 134 88 201 777.6 0 201 89 306 777.6 0 306 90 65 777.6 0 65 91 68 777.6 0 68 92 31 777.6 0 31 93 70 777.6 0 70 94 325 777.6 0 325 95 876 777.6 0 876 96 556 777.6 0 556 97 135 777.6 0 135 98 53 777.6 0 53 99 61 777.6 0 61 100 266 777.6 0 266 101 253 777.6 0 253 102 138 777.6 0 138 103 73 777.6 0 73 104 23 777.6 0 23 105 28 777.6 0 28 106 49 777.6 0 49 107 44 777.6 0 44 108 25 777.6 0 25 109 27 777.6 0 27 110 75 777.6 0 75 111 63 777.6 0 63 112 49 777.6 0 49 113 86 777.6 0 86 114 84 777.6 0 84 115 58 777.6 0 58 116 147 777.6 0 147 117 262 777.6 0 262 118 291 777.6 0 291 119 176 777.6 0 176 120 135 777.6 0 135 121 123 777.6 0 123 122 135 777.6 0 135 123 92 777.6 0 92 124 139 777.6 0 139 125 226 777.6 0 226 126 206 777.6 0 206 127 202 777.6 0 202 128 110 777.6 0 110 129 105 777.6 0 105 130 106 777.6 0 106 131 208 777.6 0 208 132 310 777.6 0 310 133 358 777.6 0 358 134 317 777.6 0 317 135 356 777.6 0 356 136 324 777.6 0 324 137 409 777.6 0 409 138 216 777.6 0 216 139 206 777.6 0 206 140 162 777.6 0 162 141 149 777.6 0 149 142 86 777.6 0 86 143 56 777.6 0 56 144 107 777.6 0 107 145 174 777.6 0 174 146 252 777.6 0 252 147 160 777.6 0 160 148 134 777.6 0 134 149 95 777.6 0 95 150 111 777.6 0 111 151 194 777.6 0 194 152 210 777.6 0 210 153 134 777.6 0 134 154 280 777.6 0 280 155 624 777.6 0 624 156 196 777.6 0 196 157 334 777.6 0 334 158 222 777.6 0 222 159 138 777.6 0 138 160 106 777.6 0 106 161 104 777.6 0 104 162 110 777.6 0 110 163 90 777.6 0 90 164 188 777.6 0 188 165 118 777.6 0 118 166 165 777.6 0 165 167 407 777.6 0 407 168 138 777.6 0 138 169 168 777.6 0 168 170 200 777.6 0 200 171 75 777.6 0 75 172 64 777.6 0 64 173 142 777.6 0 142 174 491 777.6 0 491 175 238 777.6 0 238 176 198 777.6 0 198 177 263 777.6 0 263 178 284 777.6 0 284 179 307 777.6 0 307 180 1963 777.6 0 1963 181 1256 777.6 0 1256 182 725 777.6 0 725 183 196 777.6 0 196 184 200 777.6 0 200 185 233 777.6 0 233 186 286 777.6 0 286 187 221 777.6 0 221 188 99 777.6 0 99 189 61 777.6 0 61 190 188 777.6 0 188 191 264 777.6 0 264 192 320 777.6 0 320 193 302 777.6 0 302 194 931 777.6 0 931 195 2116 777.6 0 2116 196 268 777.6 0 268 197 287 777.6 0 287 198 671 777.6 0 671 199 565 777.6 0 565 200 804 777.6 0 804 201 806 777.6 0 806 202 4957 777.6 0 4957 203 1864 777.6 0 1864 204 113 777.6 0 113 205 177 777.6 0 177 206 190 777.6 0 190 207 95 777.6 0 95 208 61 777.6 0 61 209 58 777.6 0 58 210 77 777.6 0 77 211 122 777.6 0 122 212 162 777.6 0 162 213 194 777.6 0 194 214 147 777.6 0 147 215 92 777.6 0 92 216 102 777.6 0 102 217 107 777.6 0 107 218 141 777.6 0 141 219 208 777.6 0 208 220 122 777.6 0 122 221 71 777.6 0 71 222 92 777.6 0 92 223 212 777.6 0 212 224 153 777.6 0 153 225 98 777.6 0 98 226 99 777.6 0 99 227 214 777.6 0 214 228 167 777.6 0 167 229 131 777.6 0 131 230 155 777.6 0 155 231 98 777.6 0 98 232 55 777.6 0 55 233 46 777.6 0 46 234 95 777.6 0 95 235 189 777.6 0 189 236 150 777.6 0 150 237 110 777.6 0 110 238 137 777.6 0 137 239 170 777.6 0 170 240 164 777.6 0 164 241 74 777.6 0 74 242 76 777.6 0 76 243 90 777.6 0 90 244 77 777.6 0 77 245 148 777.6 0 148 246 121 777.6 0 121 247 150 777.6 0 150 248 177 777.6 0 177 249 1004 777.6 0 1004 250 299 777.6 0 299 251 116 777.6 0 116 252 116 777.6 0 116 253 115 777.6 0 115 254 88 777.6 0 88 255 72 777.6 0 72 256 142 777.6 0 142 257 517 777.6 0 517 258 306 777.6 0 306 259 311 777.6 0 311 260 382 777.6 0 382 261 173 777.6 0 173 262 100 777.6 0 100 263 197 777.6 0 197 264 286 777.6 0 286 265 247 777.6 0 247 266 463 777.6 0 463 267 221 777.6 0 221 268 196 777.6 0 196 269 202 777.6 0 202 270 78 777.6 0 78 271 151 777.6 0 151 272 116 777.6 0 116 273 168 777.6 0 168 274 725 777.6 0 725 275 390 777.6 0 390 276 572 777.6 0 572 277 382 777.6 0 382 278 327 777.6 0 327 279 168 777.6 0 168 280 172 777.6 0 172 281 77 777.6 0 77 282 77 777.6 0 77 283 45 777.6 0 45 284 51 777.6 0 51 285 98 777.6 0 98 286 128 777.6 0 128 287 229 777.6 0 229 288 193 777.6 0 193 289 196 777.6 0 196 290 234 777.6 0 234 291 611 777.6 0 611 292 1895 777.6 0 1895 293 392 777.6 0 392 294 250 777.6 0 250 295 216 777.6 0 216 296 180 777.6 0 180 297 231 777.6 0 231 298 203 777.6 0 203 299 2647 777.6 0 2647 300 938 777.6 0 938 301 633 777.6 0 633 Finished in 3.26 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 72,300 Reads with adapters: 30,275 (41.9%) Reads that were too short: 50 (0.1%) Reads written (passing filters): 30,225 (41.8%) Total basepairs processed: 16,335,614 bp Total written (filtered): 8,304,266 bp (50.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30275 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 12 0.0 3 0 0 5 7 16 325 0.0 3 0 300 20 5 17 26887 0.0 3 25911 867 82 27 18 2710 0.0 3 2552 134 17 7 19 41 0.0 3 1 8 12 20 20 33 0.0 3 1 0 10 22 21 142 0.0 3 128 7 1 6 22 1 0.0 3 1 27 1 0.0 3 1 29 1 0.0 3 1 32 1 0.0 3 0 0 0 1 36 6 0.0 3 0 0 0 6 38 1 0.0 3 0 0 0 1 39 12 0.0 3 0 0 1 11 55 1 0.0 3 0 0 0 1 57 6 0.0 3 0 0 0 6 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 95 2 0.0 3 0 0 0 2 103 6 0.0 3 0 0 0 6 106 4 0.0 3 0 0 0 4 107 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 4 0.0 3 0 0 0 4 120 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 6 0.0 3 0 0 2 4 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 1 157 1 0.0 3 0 0 1 158 1 0.0 3 0 0 0 1 160 19 0.0 3 0 0 0 19 161 2 0.0 3 0 0 0 2 163 10 0.0 3 0 0 1 9 164 2 0.0 3 0 0 0 2 176 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 262 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 1 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.36 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 30,225 Reads with adapters: 1,237 (4.1%) Reads written (passing filters): 30,225 (100.0%) Total basepairs processed: 8,304,266 bp Total written (filtered): 8,300,323 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1237 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.1% C: 15.5% G: 18.6% T: 23.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1007 472.3 0 1007 4 229 118.1 0 229 6 1 7.4 0 1