This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.94 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 57,980 (1.8%) Reads that were too short: 5,997 (0.2%) Reads written (passing filters): 51,983 (1.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 11,979,455 bp (1.2%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 57980 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 778 777.6 0 778 7 29819 777.6 0 29819 8 46 777.6 0 46 9 40 777.6 0 40 10 13 777.6 0 13 11 11 777.6 0 11 12 10 777.6 0 10 13 14 777.6 0 14 14 13 777.6 0 13 15 14 777.6 0 14 16 15 777.6 0 15 17 14 777.6 0 14 18 21 777.6 0 21 19 16 777.6 0 16 20 18 777.6 0 18 21 11 777.6 0 11 22 18 777.6 0 18 23 10 777.6 0 10 24 23 777.6 0 23 25 14 777.6 0 14 26 19 777.6 0 19 27 14 777.6 0 14 28 15 777.6 0 15 29 10 777.6 0 10 30 10 777.6 0 10 31 11 777.6 0 11 32 10 777.6 0 10 33 17 777.6 0 17 34 15 777.6 0 15 35 17 777.6 0 17 36 13 777.6 0 13 37 1263 777.6 0 1263 38 14 777.6 0 14 39 15 777.6 0 15 40 17 777.6 0 17 41 19 777.6 0 19 42 68 777.6 0 68 43 38 777.6 0 38 44 55 777.6 0 55 45 10 777.6 0 10 46 7 777.6 0 7 47 15 777.6 0 15 48 10 777.6 0 10 49 6 777.6 0 6 50 9 777.6 0 9 51 9 777.6 0 9 52 11 777.6 0 11 53 8 777.6 0 8 54 12 777.6 0 12 55 14 777.6 0 14 56 16 777.6 0 16 57 5 777.6 0 5 58 8 777.6 0 8 59 10 777.6 0 10 60 10 777.6 0 10 61 10 777.6 0 10 62 13 777.6 0 13 63 6 777.6 0 6 64 12 777.6 0 12 65 15 777.6 0 15 66 24 777.6 0 24 67 14 777.6 0 14 68 8 777.6 0 8 69 7 777.6 0 7 70 4 777.6 0 4 71 9 777.6 0 9 72 6 777.6 0 6 73 9 777.6 0 9 74 17 777.6 0 17 75 29 777.6 0 29 76 9 777.6 0 9 77 18 777.6 0 18 78 4 777.6 0 4 79 69 777.6 0 69 80 32 777.6 0 32 81 6 777.6 0 6 82 8 777.6 0 8 83 7 777.6 0 7 84 6 777.6 0 6 85 8 777.6 0 8 86 11 777.6 0 11 87 5 777.6 0 5 88 4 777.6 0 4 89 23 777.6 0 23 90 14 777.6 0 14 91 28 777.6 0 28 92 322 777.6 0 322 93 382 777.6 0 382 94 285 777.6 0 285 95 6 777.6 0 6 96 16 777.6 0 16 97 11 777.6 0 11 98 5 777.6 0 5 99 16 777.6 0 16 100 7 777.6 0 7 101 4 777.6 0 4 102 14 777.6 0 14 103 11 777.6 0 11 104 8 777.6 0 8 105 11 777.6 0 11 106 14 777.6 0 14 107 17 777.6 0 17 108 17 777.6 0 17 109 17 777.6 0 17 110 45 777.6 0 45 111 12 777.6 0 12 112 16 777.6 0 16 113 30 777.6 0 30 114 116 777.6 0 116 115 17 777.6 0 17 116 19 777.6 0 19 117 16 777.6 0 16 118 25 777.6 0 25 119 22 777.6 0 22 120 28 777.6 0 28 121 32 777.6 0 32 122 51 777.6 0 51 123 1058 777.6 0 1058 124 636 777.6 0 636 125 655 777.6 0 655 126 6 777.6 0 6 127 14 777.6 0 14 128 10 777.6 0 10 129 16 777.6 0 16 130 12 777.6 0 12 131 70 777.6 0 70 132 14 777.6 0 14 133 14 777.6 0 14 134 7 777.6 0 7 135 14 777.6 0 14 136 8 777.6 0 8 137 9 777.6 0 9 138 17 777.6 0 17 139 13 777.6 0 13 140 7 777.6 0 7 141 17 777.6 0 17 142 6 777.6 0 6 143 9 777.6 0 9 144 25 777.6 0 25 145 7 777.6 0 7 146 12 777.6 0 12 147 65 777.6 0 65 148 41 777.6 0 41 149 6 777.6 0 6 150 4 777.6 0 4 151 13 777.6 0 13 152 3 777.6 0 3 153 7 777.6 0 7 154 12 777.6 0 12 155 7 777.6 0 7 156 7 777.6 0 7 157 47 777.6 0 47 158 4 777.6 0 4 159 28 777.6 0 28 160 40 777.6 0 40 161 16 777.6 0 16 162 10 777.6 0 10 163 8 777.6 0 8 164 12 777.6 0 12 165 14 777.6 0 14 166 18 777.6 0 18 167 26 777.6 0 26 168 12 777.6 0 12 169 21 777.6 0 21 170 44 777.6 0 44 171 17 777.6 0 17 172 33 777.6 0 33 173 41 777.6 0 41 174 223 777.6 0 223 175 354 777.6 0 354 176 130 777.6 0 130 177 70 777.6 0 70 178 37 777.6 0 37 179 83 777.6 0 83 180 99 777.6 0 99 181 104 777.6 0 104 182 26 777.6 0 26 183 28 777.6 0 28 184 100 777.6 0 100 185 53 777.6 0 53 186 86 777.6 0 86 187 81 777.6 0 81 188 90 777.6 0 90 189 15 777.6 0 15 190 3 777.6 0 3 191 19 777.6 0 19 192 42 777.6 0 42 193 80 777.6 0 80 194 268 777.6 0 268 195 118 777.6 0 118 196 18 777.6 0 18 197 13 777.6 0 13 198 544 777.6 0 544 199 3036 777.6 0 3036 200 8159 777.6 0 8159 201 147 777.6 0 147 202 9 777.6 0 9 203 7 777.6 0 7 204 12 777.6 0 12 205 20 777.6 0 20 206 31 777.6 0 31 207 15 777.6 0 15 208 12 777.6 0 12 209 8 777.6 0 8 210 4 777.6 0 4 211 14 777.6 0 14 212 13 777.6 0 13 213 19 777.6 0 19 214 184 777.6 0 184 215 11 777.6 0 11 216 12 777.6 0 12 217 14 777.6 0 14 218 13 777.6 0 13 219 74 777.6 0 74 220 117 777.6 0 117 221 49 777.6 0 49 222 170 777.6 0 170 223 110 777.6 0 110 224 45 777.6 0 45 225 23 777.6 0 23 226 16 777.6 0 16 227 19 777.6 0 19 228 26 777.6 0 26 229 13 777.6 0 13 230 13 777.6 0 13 231 13 777.6 0 13 232 7 777.6 0 7 233 9 777.6 0 9 234 12 777.6 0 12 235 10 777.6 0 10 236 28 777.6 0 28 237 50 777.6 0 50 238 101 777.6 0 101 239 244 777.6 0 244 240 1818 777.6 0 1818 241 1040 777.6 0 1040 242 140 777.6 0 140 243 7 777.6 0 7 244 6 777.6 0 6 245 15 777.6 0 15 246 37 777.6 0 37 247 34 777.6 0 34 248 58 777.6 0 58 249 19 777.6 0 19 250 12 777.6 0 12 251 8 777.6 0 8 252 10 777.6 0 10 253 10 777.6 0 10 254 14 777.6 0 14 255 12 777.6 0 12 256 26 777.6 0 26 257 67 777.6 0 67 258 92 777.6 0 92 259 14 777.6 0 14 260 23 777.6 0 23 261 247 777.6 0 247 262 12 777.6 0 12 263 13 777.6 0 13 264 11 777.6 0 11 265 16 777.6 0 16 266 13 777.6 0 13 267 27 777.6 0 27 268 15 777.6 0 15 269 14 777.6 0 14 270 5 777.6 0 5 271 3 777.6 0 3 272 3 777.6 0 3 273 7 777.6 0 7 274 9 777.6 0 9 275 26 777.6 0 26 276 10 777.6 0 10 277 4 777.6 0 4 278 7 777.6 0 7 279 14 777.6 0 14 280 20 777.6 0 20 281 10 777.6 0 10 282 8 777.6 0 8 283 10 777.6 0 10 284 10 777.6 0 10 285 15 777.6 0 15 286 64 777.6 0 64 287 166 777.6 0 166 288 99 777.6 0 99 289 21 777.6 0 21 290 22 777.6 0 22 291 10 777.6 0 10 292 11 777.6 0 11 293 13 777.6 0 13 294 11 777.6 0 11 295 6 777.6 0 6 296 7 777.6 0 7 297 5 777.6 0 5 298 2 777.6 0 2 299 4 777.6 0 4 300 10 777.6 0 10 301 8 777.6 0 8 Finished in 2.49 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 51,983 Reads with adapters: 30,446 (58.6%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 30,443 (58.6%) Total basepairs processed: 11,979,455 bp Total written (filtered): 8,341,791 bp (69.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30446 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 3 0.0 3 0 0 0 0 3 18 13 0.0 3 0 0 13 19 387 0.0 3 0 364 13 4 6 20 29953 0.0 4 29458 469 16 5 5 21 58 0.0 4 9 45 2 0 2 22 4 0.0 4 0 1 1 1 1 23 14 0.0 4 0 0 0 14 24 6 0.0 4 0 0 0 0 6 54 1 0.0 4 0 0 0 0 1 86 4 0.0 4 0 0 0 0 4 91 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.29 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 30,443 Reads with adapters: 184 (0.6%) Reads written (passing filters): 30,443 (100.0%) Total basepairs processed: 8,341,791 bp Total written (filtered): 8,330,175 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 184 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.0% C: 27.7% G: 64.7% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 15 475.7 0 15 11 114 0.0 1 1 113 12 9 0.0 1 4 5 152 2 0.0 1 0 2 225 39 0.0 1 38 1 226 5 0.0 1 5