This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 108.63 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 663,506 (20.8%) Reads that were too short: 34,551 (1.1%) Reads written (passing filters): 628,955 (19.7%) Total basepairs processed: 958,672,960 bp Total written (filtered): 177,320,098 bp (18.5%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 663506 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2728 777.6 0 2728 7 48218 777.6 0 48218 8 5951 777.6 0 5951 9 165948 777.6 0 165948 10 3498 777.6 0 3498 11 4507 777.6 0 4507 12 75862 777.6 0 75862 13 133961 777.6 0 133961 14 149903 777.6 0 149903 15 305 777.6 0 305 16 16 777.6 0 16 17 8 777.6 0 8 18 10 777.6 0 10 19 12 777.6 0 12 20 7 777.6 0 7 21 5 777.6 0 5 22 11 777.6 0 11 23 7 777.6 0 7 24 16 777.6 0 16 25 11 777.6 0 11 26 20 777.6 0 20 27 19 777.6 0 19 28 18 777.6 0 18 29 11 777.6 0 11 30 15 777.6 0 15 31 11 777.6 0 11 32 20 777.6 0 20 33 11 777.6 0 11 34 17 777.6 0 17 35 15 777.6 0 15 36 58 777.6 0 58 37 18 777.6 0 18 38 240 777.6 0 240 39 10 777.6 0 10 40 33 777.6 0 33 41 6 777.6 0 6 42 7 777.6 0 7 43 57 777.6 0 57 44 13 777.6 0 13 45 18 777.6 0 18 46 5 777.6 0 5 47 5 777.6 0 5 48 4 777.6 0 4 49 6 777.6 0 6 50 6 777.6 0 6 51 8 777.6 0 8 52 11 777.6 0 11 53 5 777.6 0 5 54 6 777.6 0 6 55 2 777.6 0 2 56 10 777.6 0 10 57 7 777.6 0 7 58 14 777.6 0 14 59 9 777.6 0 9 60 8 777.6 0 8 61 7 777.6 0 7 62 6 777.6 0 6 63 9 777.6 0 9 64 6 777.6 0 6 65 4 777.6 0 4 66 5 777.6 0 5 67 9 777.6 0 9 68 5 777.6 0 5 69 3 777.6 0 3 70 4 777.6 0 4 71 5 777.6 0 5 72 23 777.6 0 23 73 254 777.6 0 254 74 35 777.6 0 35 75 49 777.6 0 49 76 30 777.6 0 30 77 90 777.6 0 90 78 52 777.6 0 52 79 108 777.6 0 108 80 64 777.6 0 64 81 129 777.6 0 129 82 56 777.6 0 56 83 92 777.6 0 92 84 84 777.6 0 84 85 45 777.6 0 45 86 108 777.6 0 108 87 109 777.6 0 109 88 39 777.6 0 39 89 135 777.6 0 135 90 418 777.6 0 418 91 319 777.6 0 319 92 66 777.6 0 66 93 188 777.6 0 188 94 412 777.6 0 412 95 257 777.6 0 257 96 406 777.6 0 406 97 253 777.6 0 253 98 101 777.6 0 101 99 41 777.6 0 41 100 102 777.6 0 102 101 137 777.6 0 137 102 83 777.6 0 83 103 129 777.6 0 129 104 233 777.6 0 233 105 205 777.6 0 205 106 1643 777.6 0 1643 107 442 777.6 0 442 108 174 777.6 0 174 109 86 777.6 0 86 110 377 777.6 0 377 111 228 777.6 0 228 112 292 777.6 0 292 113 311 777.6 0 311 114 113 777.6 0 113 115 120 777.6 0 120 116 83 777.6 0 83 117 62 777.6 0 62 118 69 777.6 0 69 119 152 777.6 0 152 120 572 777.6 0 572 121 245 777.6 0 245 122 407 777.6 0 407 123 149 777.6 0 149 124 127 777.6 0 127 125 185 777.6 0 185 126 578 777.6 0 578 127 1843 777.6 0 1843 128 1533 777.6 0 1533 129 784 777.6 0 784 130 161 777.6 0 161 131 109 777.6 0 109 132 123 777.6 0 123 133 185 777.6 0 185 134 297 777.6 0 297 135 568 777.6 0 568 136 348 777.6 0 348 137 285 777.6 0 285 138 393 777.6 0 393 139 686 777.6 0 686 140 942 777.6 0 942 141 432 777.6 0 432 142 360 777.6 0 360 143 391 777.6 0 391 144 132 777.6 0 132 145 317 777.6 0 317 146 664 777.6 0 664 147 220 777.6 0 220 148 335 777.6 0 335 149 85 777.6 0 85 150 121 777.6 0 121 151 598 777.6 0 598 152 349 777.6 0 349 153 734 777.6 0 734 154 375 777.6 0 375 155 366 777.6 0 366 156 141 777.6 0 141 157 172 777.6 0 172 158 1028 777.6 0 1028 159 406 777.6 0 406 160 476 777.6 0 476 161 629 777.6 0 629 162 271 777.6 0 271 163 272 777.6 0 272 164 193 777.6 0 193 165 281 777.6 0 281 166 284 777.6 0 284 167 273 777.6 0 273 168 171 777.6 0 171 169 110 777.6 0 110 170 201 777.6 0 201 171 93 777.6 0 93 172 135 777.6 0 135 173 228 777.6 0 228 174 635 777.6 0 635 175 221 777.6 0 221 176 216 777.6 0 216 177 131 777.6 0 131 178 85 777.6 0 85 179 175 777.6 0 175 180 240 777.6 0 240 181 366 777.6 0 366 182 314 777.6 0 314 183 322 777.6 0 322 184 234 777.6 0 234 185 269 777.6 0 269 186 246 777.6 0 246 187 183 777.6 0 183 188 157 777.6 0 157 189 93 777.6 0 93 190 207 777.6 0 207 191 339 777.6 0 339 192 578 777.6 0 578 193 190 777.6 0 190 194 249 777.6 0 249 195 125 777.6 0 125 196 92 777.6 0 92 197 108 777.6 0 108 198 402 777.6 0 402 199 319 777.6 0 319 200 186 777.6 0 186 201 136 777.6 0 136 202 195 777.6 0 195 203 329 777.6 0 329 204 307 777.6 0 307 205 170 777.6 0 170 206 296 777.6 0 296 207 279 777.6 0 279 208 266 777.6 0 266 209 244 777.6 0 244 210 257 777.6 0 257 211 325 777.6 0 325 212 174 777.6 0 174 213 220 777.6 0 220 214 349 777.6 0 349 215 295 777.6 0 295 216 209 777.6 0 209 217 203 777.6 0 203 218 213 777.6 0 213 219 324 777.6 0 324 220 491 777.6 0 491 221 228 777.6 0 228 222 129 777.6 0 129 223 150 777.6 0 150 224 483 777.6 0 483 225 451 777.6 0 451 226 300 777.6 0 300 227 258 777.6 0 258 228 280 777.6 0 280 229 167 777.6 0 167 230 160 777.6 0 160 231 174 777.6 0 174 232 186 777.6 0 186 233 207 777.6 0 207 234 280 777.6 0 280 235 295 777.6 0 295 236 288 777.6 0 288 237 183 777.6 0 183 238 185 777.6 0 185 239 131 777.6 0 131 240 313 777.6 0 313 241 301 777.6 0 301 242 294 777.6 0 294 243 343 777.6 0 343 244 311 777.6 0 311 245 471 777.6 0 471 246 335 777.6 0 335 247 237 777.6 0 237 248 157 777.6 0 157 249 112 777.6 0 112 250 75 777.6 0 75 251 153 777.6 0 153 252 223 777.6 0 223 253 267 777.6 0 267 254 319 777.6 0 319 255 392 777.6 0 392 256 582 777.6 0 582 257 387 777.6 0 387 258 232 777.6 0 232 259 131 777.6 0 131 260 142 777.6 0 142 261 229 777.6 0 229 262 331 777.6 0 331 263 202 777.6 0 202 264 261 777.6 0 261 265 241 777.6 0 241 266 569 777.6 0 569 267 980 777.6 0 980 268 530 777.6 0 530 269 317 777.6 0 317 270 156 777.6 0 156 271 226 777.6 0 226 272 243 777.6 0 243 273 219 777.6 0 219 274 303 777.6 0 303 275 244 777.6 0 244 276 208 777.6 0 208 277 597 777.6 0 597 278 1208 777.6 0 1208 279 901 777.6 0 901 280 1249 777.6 0 1249 281 1734 777.6 0 1734 282 1139 777.6 0 1139 283 652 777.6 0 652 284 340 777.6 0 340 285 409 777.6 0 409 286 352 777.6 0 352 287 566 777.6 0 566 288 586 777.6 0 586 289 407 777.6 0 407 290 180 777.6 0 180 291 164 777.6 0 164 292 263 777.6 0 263 293 415 777.6 0 415 294 539 777.6 0 539 295 345 777.6 0 345 296 330 777.6 0 330 297 340 777.6 0 340 298 239 777.6 0 239 299 371 777.6 0 371 300 272 777.6 0 272 301 236 777.6 0 236 Finished in 33.65 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 628,955 Reads with adapters: 45,619 (7.3%) Reads that were too short: 625 (0.1%) Reads written (passing filters): 44,994 (7.2%) Total basepairs processed: 177,320,098 bp Total written (filtered): 12,171,986 bp (6.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 45619 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 9 1 16 322 0.0 3 0 292 18 12 17 42480 0.0 3 41301 998 144 37 18 99 0.0 3 6 86 3 4 19 61 0.0 3 0 1 44 16 20 113 0.0 3 81 1 1 30 22 2 0.0 3 1 0 0 1 23 1 0.0 3 1 24 1 0.0 3 1 25 1 0.0 3 1 26 1 0.0 3 1 28 1 0.0 3 1 29 1 0.0 3 1 30 2 0.0 3 1 0 1 31 3 0.0 3 1 1 0 1 32 3 0.0 3 1 0 0 2 33 2 0.0 3 2 37 1 0.0 3 0 1 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 43 16 0.0 3 0 0 0 16 45 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 50 1 0.0 3 0 1 51 6 0.0 3 0 0 0 6 53 10 0.0 3 0 0 0 10 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 63 1 0.0 3 0 0 0 1 67 7 0.0 3 0 0 0 7 68 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 3 0.0 3 0 0 0 3 72 1 0.0 3 0 0 0 1 73 1 0.0 3 1 84 1 0.0 3 0 0 0 1 87 28 0.0 3 0 0 0 28 88 4 0.0 3 0 0 0 4 89 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 103 1 0.0 3 1 106 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 35 0.0 3 0 0 0 35 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 1 113 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 3 0.0 3 0 0 0 3 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 5 0.0 3 0 0 0 5 140 2 0.0 3 0 0 0 2 141 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 39 0.0 3 0 0 0 39 155 5 0.0 3 0 0 0 5 156 6 0.0 3 0 0 0 6 157 8 0.0 3 0 0 0 8 158 10 0.0 3 0 0 0 10 159 502 0.0 3 0 0 3 499 160 39 0.0 3 0 0 0 39 161 62 0.0 3 0 0 1 61 162 85 0.0 3 0 0 1 84 163 19 0.0 3 0 0 0 19 164 47 0.0 3 0 0 0 47 165 15 0.0 3 0 0 0 15 167 2 0.0 3 0 0 0 2 168 3 0.0 3 1 0 1 1 171 13 0.0 3 0 0 0 13 172 5 0.0 3 0 0 0 5 173 2 0.0 3 0 0 0 2 174 10 0.0 3 0 0 0 10 175 2 0.0 3 0 0 0 2 176 5 0.0 3 0 0 0 5 177 6 0.0 3 0 0 0 6 178 73 0.0 3 0 0 0 73 179 428 0.0 3 0 0 0 428 180 116 0.0 3 0 0 0 116 181 9 0.0 3 0 0 0 9 182 187 0.0 3 0 0 3 184 183 19 0.0 3 0 0 1 18 185 9 0.0 3 0 0 1 8 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 191 2 0.0 3 0 0 0 2 192 8 0.0 3 0 0 0 8 193 1 0.0 3 0 0 0 1 198 5 0.0 3 0 0 0 5 202 2 0.0 3 0 0 0 2 205 5 0.0 3 0 0 0 5 206 2 0.0 3 0 0 1 1 210 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 212 2 0.0 3 0 0 0 2 214 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 1 230 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 239 9 0.0 3 0 0 0 9 240 2 0.0 3 0 0 0 2 242 1 0.0 3 0 0 0 1 246 3 0.0 3 0 0 0 3 247 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 255 4 0.0 3 0 0 1 3 256 2 0.0 3 0 0 0 2 259 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 262 2 0.0 3 0 0 0 2 264 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 266 2 0.0 3 0 0 0 2 267 1 0.0 3 0 0 0 1 268 2 0.0 3 0 0 0 2 269 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 76 0.0 3 0 0 1 75 272 30 0.0 3 0 0 1 29 273 14 0.0 3 0 0 0 14 274 48 0.0 3 0 0 1 47 275 51 0.0 3 0 0 1 50 276 7 0.0 3 0 0 0 7 277 11 0.0 3 0 0 1 10 278 4 0.0 3 0 0 0 4 279 12 0.0 3 0 0 0 12 280 44 0.0 3 0 0 1 43 281 164 0.0 3 0 0 5 159 282 44 0.0 3 0 0 0 44 283 15 0.0 3 0 0 1 14 284 11 0.0 3 0 0 0 11 285 3 0.0 3 0 0 0 3 286 2 0.0 3 0 0 0 2 287 2 0.0 3 0 0 0 2 288 6 0.0 3 0 0 0 6 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.03 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 44,994 Reads with adapters: 3,027 (6.7%) Reads written (passing filters): 44,994 (100.0%) Total basepairs processed: 12,171,986 bp Total written (filtered): 12,162,657 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3027 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.2% C: 53.9% G: 9.1% T: 15.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2803 703.0 0 2803 4 203 175.8 0 203 5 19 43.9 0 19 6 1 11.0 0 1 7 1 2.7 0 1