This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 106.62 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 136,968 (4.3%) Reads that were too short: 64,583 (2.0%) Reads written (passing filters): 72,385 (2.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 18,940,390 bp (2.0%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 136968 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 836 777.6 0 836 7 760 777.6 0 760 8 1458 777.6 0 1458 9 42089 777.6 0 42089 10 389 777.6 0 389 11 7227 777.6 0 7227 12 22 777.6 0 22 13 13 777.6 0 13 14 12 777.6 0 12 15 14 777.6 0 14 16 8 777.6 0 8 17 7 777.6 0 7 18 13 777.6 0 13 19 7 777.6 0 7 20 11 777.6 0 11 21 11 777.6 0 11 22 11 777.6 0 11 23 13 777.6 0 13 24 13 777.6 0 13 25 5 777.6 0 5 26 10 777.6 0 10 27 13 777.6 0 13 28 23 777.6 0 23 29 17 777.6 0 17 30 6 777.6 0 6 31 7 777.6 0 7 32 5 777.6 0 5 33 12 777.6 0 12 34 10 777.6 0 10 35 12 777.6 0 12 36 13 777.6 0 13 37 10 777.6 0 10 38 11 777.6 0 11 39 12 777.6 0 12 40 4 777.6 0 4 41 8 777.6 0 8 42 18 777.6 0 18 43 9 777.6 0 9 44 18 777.6 0 18 45 8 777.6 0 8 46 15 777.6 0 15 47 15 777.6 0 15 48 6 777.6 0 6 49 9 777.6 0 9 50 13 777.6 0 13 51 8 777.6 0 8 52 56 777.6 0 56 53 19 777.6 0 19 54 27 777.6 0 27 55 53 777.6 0 53 56 67 777.6 0 67 57 47 777.6 0 47 58 42 777.6 0 42 59 15 777.6 0 15 60 10 777.6 0 10 61 14 777.6 0 14 62 26 777.6 0 26 63 7 777.6 0 7 64 22 777.6 0 22 65 16 777.6 0 16 66 10 777.6 0 10 67 10 777.6 0 10 68 6 777.6 0 6 69 6 777.6 0 6 70 25 777.6 0 25 71 14 777.6 0 14 72 16 777.6 0 16 73 16 777.6 0 16 74 13 777.6 0 13 75 3 777.6 0 3 76 11 777.6 0 11 77 7 777.6 0 7 78 19 777.6 0 19 79 178 777.6 0 178 80 182 777.6 0 182 81 78 777.6 0 78 82 16 777.6 0 16 83 140 777.6 0 140 84 41 777.6 0 41 85 228 777.6 0 228 86 8 777.6 0 8 87 16 777.6 0 16 88 7 777.6 0 7 89 22 777.6 0 22 90 6 777.6 0 6 91 11 777.6 0 11 92 10 777.6 0 10 93 12 777.6 0 12 94 17 777.6 0 17 95 26 777.6 0 26 96 20 777.6 0 20 97 38 777.6 0 38 98 47 777.6 0 47 99 90 777.6 0 90 100 197 777.6 0 197 101 50 777.6 0 50 102 47 777.6 0 47 103 92 777.6 0 92 104 57 777.6 0 57 105 53 777.6 0 53 106 88 777.6 0 88 107 70 777.6 0 70 108 93 777.6 0 93 109 56 777.6 0 56 110 95 777.6 0 95 111 101 777.6 0 101 112 209 777.6 0 209 113 3639 777.6 0 3639 114 4233 777.6 0 4233 115 2789 777.6 0 2789 116 39 777.6 0 39 117 13 777.6 0 13 118 10 777.6 0 10 119 21 777.6 0 21 120 10 777.6 0 10 121 20 777.6 0 20 122 16 777.6 0 16 123 11 777.6 0 11 124 14 777.6 0 14 125 10 777.6 0 10 126 12 777.6 0 12 127 22 777.6 0 22 128 32 777.6 0 32 129 474 777.6 0 474 130 268 777.6 0 268 131 97 777.6 0 97 132 19 777.6 0 19 133 11 777.6 0 11 134 8 777.6 0 8 135 18 777.6 0 18 136 28 777.6 0 28 137 17 777.6 0 17 138 19 777.6 0 19 139 26 777.6 0 26 140 30 777.6 0 30 141 64 777.6 0 64 142 29 777.6 0 29 143 129 777.6 0 129 144 29 777.6 0 29 145 23 777.6 0 23 146 38 777.6 0 38 147 30 777.6 0 30 148 25 777.6 0 25 149 48 777.6 0 48 150 23 777.6 0 23 151 42 777.6 0 42 152 23 777.6 0 23 153 41 777.6 0 41 154 188 777.6 0 188 155 397 777.6 0 397 156 656 777.6 0 656 157 193 777.6 0 193 158 691 777.6 0 691 159 16 777.6 0 16 160 9 777.6 0 9 161 7 777.6 0 7 162 9 777.6 0 9 163 12 777.6 0 12 164 12 777.6 0 12 165 14 777.6 0 14 166 28 777.6 0 28 167 332 777.6 0 332 168 79 777.6 0 79 169 163 777.6 0 163 170 8 777.6 0 8 171 12 777.6 0 12 172 15 777.6 0 15 173 12 777.6 0 12 174 17 777.6 0 17 175 10 777.6 0 10 176 8 777.6 0 8 177 9 777.6 0 9 178 4 777.6 0 4 179 14 777.6 0 14 180 12 777.6 0 12 181 18 777.6 0 18 182 9 777.6 0 9 183 16 777.6 0 16 184 7 777.6 0 7 185 7 777.6 0 7 186 9 777.6 0 9 187 7 777.6 0 7 188 13 777.6 0 13 189 26 777.6 0 26 190 11 777.6 0 11 191 22 777.6 0 22 192 19 777.6 0 19 193 7 777.6 0 7 194 9 777.6 0 9 195 12 777.6 0 12 196 14 777.6 0 14 197 19 777.6 0 19 198 20 777.6 0 20 199 30 777.6 0 30 200 429 777.6 0 429 201 115 777.6 0 115 202 41 777.6 0 41 203 9 777.6 0 9 204 55 777.6 0 55 205 15 777.6 0 15 206 8 777.6 0 8 207 17 777.6 0 17 208 16 777.6 0 16 209 21 777.6 0 21 210 18 777.6 0 18 211 62 777.6 0 62 212 40 777.6 0 40 213 51 777.6 0 51 214 35 777.6 0 35 215 24 777.6 0 24 216 363 777.6 0 363 217 47 777.6 0 47 218 68 777.6 0 68 219 12 777.6 0 12 220 6 777.6 0 6 221 8 777.6 0 8 222 10 777.6 0 10 223 10 777.6 0 10 224 23 777.6 0 23 225 13 777.6 0 13 226 26 777.6 0 26 227 19 777.6 0 19 228 33 777.6 0 33 229 42 777.6 0 42 230 27 777.6 0 27 231 31 777.6 0 31 232 28 777.6 0 28 233 85 777.6 0 85 234 16 777.6 0 16 235 23 777.6 0 23 236 33 777.6 0 33 237 17 777.6 0 17 238 7 777.6 0 7 239 12 777.6 0 12 240 5 777.6 0 5 241 11 777.6 0 11 242 5 777.6 0 5 243 16 777.6 0 16 244 12 777.6 0 12 245 10 777.6 0 10 246 11 777.6 0 11 247 18 777.6 0 18 248 17 777.6 0 17 249 10 777.6 0 10 250 8 777.6 0 8 251 28 777.6 0 28 252 36 777.6 0 36 253 22 777.6 0 22 254 37 777.6 0 37 255 126 777.6 0 126 256 131 777.6 0 131 257 55 777.6 0 55 258 30 777.6 0 30 259 26 777.6 0 26 260 9 777.6 0 9 261 15 777.6 0 15 262 79 777.6 0 79 263 32 777.6 0 32 264 35 777.6 0 35 265 8 777.6 0 8 266 10 777.6 0 10 267 16 777.6 0 16 268 24 777.6 0 24 269 9 777.6 0 9 270 70 777.6 0 70 271 32 777.6 0 32 272 38 777.6 0 38 273 38 777.6 0 38 274 39 777.6 0 39 275 16 777.6 0 16 276 24 777.6 0 24 277 23 777.6 0 23 278 38 777.6 0 38 279 47 777.6 0 47 280 66 777.6 0 66 281 105 777.6 0 105 282 97 777.6 0 97 283 88 777.6 0 88 284 264 777.6 0 264 285 365 777.6 0 365 286 91 777.6 0 91 287 104 777.6 0 104 288 165 777.6 0 165 289 134 777.6 0 134 290 86 777.6 0 86 291 394 777.6 0 394 292 423 777.6 0 423 293 431 777.6 0 431 294 214 777.6 0 214 295 306 777.6 0 306 296 987 777.6 0 987 297 18214 777.6 0 18214 298 26524 777.6 0 26524 299 6081 777.6 0 6081 300 6807 777.6 0 6807 301 50 777.6 0 50 Finished in 3.78 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 72,385 Reads with adapters: 44,476 (61.4%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 44,475 (61.4%) Total basepairs processed: 18,940,390 bp Total written (filtered): 12,101,840 bp (63.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 44476 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 7 0.0 3 0 0 0 2 5 18 21 0.0 3 0 0 21 19 583 0.0 3 0 565 11 0 7 20 43745 0.0 4 43096 632 14 2 1 21 89 0.0 4 23 63 1 1 1 22 6 0.0 4 0 0 4 1 1 23 19 0.0 4 0 0 1 17 1 24 1 0.0 4 0 0 0 1 26 1 0.0 4 1 48 1 0.0 4 1 49 1 0.0 4 1 217 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.87 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 44,475 Reads with adapters: 1,288 (2.9%) Reads written (passing filters): 44,475 (100.0%) Total basepairs processed: 12,101,840 bp Total written (filtered): 11,918,736 bp (98.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1288 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 3.4% G: 90.8% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 694.9 0 3 4 7 173.7 0 7 5 2 43.4 0 2 6 39 10.9 0 39 9 48 0.2 0 4 44 10 237 0.0 1 7 230 11 269 0.0 1 0 269 12 1 0.0 1 0 1 13 1 0.0 1 0 1 150 1 0.0 1 0 1 209 4 0.0 1 4 223 27 0.0 1 23 4 224 5 0.0 1 4 1 238 90 0.0 1 83 7 239 4 0.0 1 4 241 1 0.0 1 1 242 2 0.0 1 2 260 15 0.0 1 14 1 261 2 0.0 1 2 262 21 0.0 1 18 3 263 1 0.0 1 1 264 16 0.0 1 16 265 3 0.0 1 3 266 2 0.0 1 2 267 450 0.0 1 426 24 268 33 0.0 1 33 269 3 0.0 1 3 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.