This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.92 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 62,707 (2.0%) Reads that were too short: 15,435 (0.5%) Reads written (passing filters): 47,272 (1.5%) Total basepairs processed: 958,672,960 bp Total written (filtered): 11,599,658 bp (1.2%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 62707 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 568 777.6 0 568 7 20352 777.6 0 20352 8 45 777.6 0 45 9 36 777.6 0 36 10 19 777.6 0 19 11 19 777.6 0 19 12 15 777.6 0 15 13 17 777.6 0 17 14 19 777.6 0 19 15 20 777.6 0 20 16 18 777.6 0 18 17 27 777.6 0 27 18 29 777.6 0 29 19 43 777.6 0 43 20 37 777.6 0 37 21 93 777.6 0 93 22 51 777.6 0 51 23 32 777.6 0 32 24 64 777.6 0 64 25 53 777.6 0 53 26 90 777.6 0 90 27 100 777.6 0 100 28 51 777.6 0 51 29 39 777.6 0 39 30 51 777.6 0 51 31 54 777.6 0 54 32 118 777.6 0 118 33 3321 777.6 0 3321 34 82 777.6 0 82 35 1430 777.6 0 1430 36 377 777.6 0 377 37 556 777.6 0 556 38 7 777.6 0 7 39 14 777.6 0 14 40 19 777.6 0 19 41 12 777.6 0 12 42 27 777.6 0 27 43 14 777.6 0 14 44 15 777.6 0 15 45 83 777.6 0 83 46 18 777.6 0 18 47 10 777.6 0 10 48 8 777.6 0 8 49 13 777.6 0 13 50 17 777.6 0 17 51 15 777.6 0 15 52 19 777.6 0 19 53 17 777.6 0 17 54 13 777.6 0 13 55 18 777.6 0 18 56 9 777.6 0 9 57 9 777.6 0 9 58 16 777.6 0 16 59 150 777.6 0 150 60 16 777.6 0 16 61 25 777.6 0 25 62 83 777.6 0 83 63 70 777.6 0 70 64 69 777.6 0 69 65 15 777.6 0 15 66 27 777.6 0 27 67 27 777.6 0 27 68 124 777.6 0 124 69 405 777.6 0 405 70 260 777.6 0 260 71 84 777.6 0 84 72 19 777.6 0 19 73 9 777.6 0 9 74 9 777.6 0 9 75 17 777.6 0 17 76 15 777.6 0 15 77 14 777.6 0 14 78 24 777.6 0 24 79 12 777.6 0 12 80 26 777.6 0 26 81 41 777.6 0 41 82 48 777.6 0 48 83 18 777.6 0 18 84 28 777.6 0 28 85 16 777.6 0 16 86 17 777.6 0 17 87 28 777.6 0 28 88 111 777.6 0 111 89 116 777.6 0 116 90 473 777.6 0 473 91 24 777.6 0 24 92 69 777.6 0 69 93 46 777.6 0 46 94 76 777.6 0 76 95 31 777.6 0 31 96 73 777.6 0 73 97 231 777.6 0 231 98 599 777.6 0 599 99 3867 777.6 0 3867 100 2948 777.6 0 2948 101 351 777.6 0 351 102 53 777.6 0 53 103 39 777.6 0 39 104 169 777.6 0 169 105 67 777.6 0 67 106 56 777.6 0 56 107 105 777.6 0 105 108 51 777.6 0 51 109 96 777.6 0 96 110 432 777.6 0 432 111 204 777.6 0 204 112 324 777.6 0 324 113 74 777.6 0 74 114 27 777.6 0 27 115 49 777.6 0 49 116 37 777.6 0 37 117 18 777.6 0 18 118 62 777.6 0 62 119 37 777.6 0 37 120 53 777.6 0 53 121 132 777.6 0 132 122 107 777.6 0 107 123 88 777.6 0 88 124 59 777.6 0 59 125 47 777.6 0 47 126 29 777.6 0 29 127 69 777.6 0 69 128 43 777.6 0 43 129 85 777.6 0 85 130 62 777.6 0 62 131 98 777.6 0 98 132 143 777.6 0 143 133 100 777.6 0 100 134 94 777.6 0 94 135 43 777.6 0 43 136 54 777.6 0 54 137 30 777.6 0 30 138 44 777.6 0 44 139 125 777.6 0 125 140 76 777.6 0 76 141 139 777.6 0 139 142 88 777.6 0 88 143 89 777.6 0 89 144 49 777.6 0 49 145 38 777.6 0 38 146 85 777.6 0 85 147 55 777.6 0 55 148 143 777.6 0 143 149 65 777.6 0 65 150 32 777.6 0 32 151 30 777.6 0 30 152 29 777.6 0 29 153 40 777.6 0 40 154 42 777.6 0 42 155 111 777.6 0 111 156 70 777.6 0 70 157 71 777.6 0 71 158 61 777.6 0 61 159 646 777.6 0 646 160 320 777.6 0 320 161 152 777.6 0 152 162 52 777.6 0 52 163 50 777.6 0 50 164 60 777.6 0 60 165 45 777.6 0 45 166 63 777.6 0 63 167 74 777.6 0 74 168 121 777.6 0 121 169 44 777.6 0 44 170 50 777.6 0 50 171 58 777.6 0 58 172 69 777.6 0 69 173 68 777.6 0 68 174 68 777.6 0 68 175 45 777.6 0 45 176 31 777.6 0 31 177 53 777.6 0 53 178 40 777.6 0 40 179 46 777.6 0 46 180 35 777.6 0 35 181 42 777.6 0 42 182 56 777.6 0 56 183 71 777.6 0 71 184 57 777.6 0 57 185 62 777.6 0 62 186 37 777.6 0 37 187 46 777.6 0 46 188 49 777.6 0 49 189 64 777.6 0 64 190 59 777.6 0 59 191 76 777.6 0 76 192 75 777.6 0 75 193 82 777.6 0 82 194 64 777.6 0 64 195 173 777.6 0 173 196 71 777.6 0 71 197 103 777.6 0 103 198 48 777.6 0 48 199 33 777.6 0 33 200 85 777.6 0 85 201 101 777.6 0 101 202 179 777.6 0 179 203 150 777.6 0 150 204 84 777.6 0 84 205 318 777.6 0 318 206 288 777.6 0 288 207 618 777.6 0 618 208 243 777.6 0 243 209 312 777.6 0 312 210 89 777.6 0 89 211 105 777.6 0 105 212 91 777.6 0 91 213 76 777.6 0 76 214 90 777.6 0 90 215 92 777.6 0 92 216 67 777.6 0 67 217 71 777.6 0 71 218 57 777.6 0 57 219 35 777.6 0 35 220 91 777.6 0 91 221 107 777.6 0 107 222 103 777.6 0 103 223 176 777.6 0 176 224 93 777.6 0 93 225 76 777.6 0 76 226 63 777.6 0 63 227 88 777.6 0 88 228 67 777.6 0 67 229 86 777.6 0 86 230 88 777.6 0 88 231 74 777.6 0 74 232 112 777.6 0 112 233 105 777.6 0 105 234 122 777.6 0 122 235 314 777.6 0 314 236 120 777.6 0 120 237 319 777.6 0 319 238 100 777.6 0 100 239 118 777.6 0 118 240 179 777.6 0 179 241 941 777.6 0 941 242 501 777.6 0 501 243 226 777.6 0 226 244 94 777.6 0 94 245 84 777.6 0 84 246 62 777.6 0 62 247 324 777.6 0 324 248 315 777.6 0 315 249 1262 777.6 0 1262 250 503 777.6 0 503 251 69 777.6 0 69 252 51 777.6 0 51 253 26 777.6 0 26 254 61 777.6 0 61 255 51 777.6 0 51 256 51 777.6 0 51 257 56 777.6 0 56 258 78 777.6 0 78 259 143 777.6 0 143 260 777 777.6 0 777 261 694 777.6 0 694 262 321 777.6 0 321 263 70 777.6 0 70 264 59 777.6 0 59 265 73 777.6 0 73 266 43 777.6 0 43 267 29 777.6 0 29 268 47 777.6 0 47 269 62 777.6 0 62 270 103 777.6 0 103 271 69 777.6 0 69 272 79 777.6 0 79 273 115 777.6 0 115 274 136 777.6 0 136 275 147 777.6 0 147 276 76 777.6 0 76 277 54 777.6 0 54 278 67 777.6 0 67 279 44 777.6 0 44 280 56 777.6 0 56 281 97 777.6 0 97 282 130 777.6 0 130 283 162 777.6 0 162 284 54 777.6 0 54 285 62 777.6 0 62 286 44 777.6 0 44 287 35 777.6 0 35 288 47 777.6 0 47 289 137 777.6 0 137 290 100 777.6 0 100 291 70 777.6 0 70 292 63 777.6 0 63 293 47 777.6 0 47 294 51 777.6 0 51 295 60 777.6 0 60 296 85 777.6 0 85 297 113 777.6 0 113 298 146 777.6 0 146 299 104 777.6 0 104 300 134 777.6 0 134 301 109 777.6 0 109 Finished in 2.48 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 47,272 Reads with adapters: 20,835 (44.1%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 20,829 (44.1%) Total basepairs processed: 11,599,658 bp Total written (filtered): 5,705,285 bp (49.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 20835 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 6 0.0 3 0 0 6 19 235 0.0 3 0 220 12 1 2 20 20474 0.0 4 20209 254 9 0 2 21 67 0.0 4 12 53 0 2 22 16 0.0 4 0 0 14 2 23 8 0.0 4 0 1 0 7 24 1 0.0 4 1 32 2 0.0 4 0 0 0 0 2 50 4 0.0 4 0 0 0 0 4 52 2 0.0 4 1 0 0 0 1 54 3 0.0 4 3 72 2 0.0 4 0 0 0 0 2 81 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 99 4 0.0 4 0 0 0 0 4 127 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 20,829 Reads with adapters: 3,395 (16.3%) Reads written (passing filters): 20,829 (100.0%) Total basepairs processed: 5,705,285 bp Total written (filtered): 4,815,454 bp (84.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3395 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.4% C: 0.1% G: 99.1% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 325.5 0 6 11 87 0.0 1 0 87 12 1 0.0 1 0 1 20 7 0.0 1 6 1 24 2 0.0 1 2 191 1 0.0 1 0 1 225 5 0.0 1 5 268 1 0.0 1 1 269 15 0.0 1 15 270 3133 0.0 1 2993 140 271 137 0.0 1 131 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.