This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.83 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 95,541 (3.0%) Reads that were too short: 64,221 (2.0%) Reads written (passing filters): 31,320 (1.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 6,823,696 bp (0.7%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 95541 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 93 777.6 0 93 7 198 777.6 0 198 8 7201 777.6 0 7201 9 4216 777.6 0 4216 10 5 777.6 0 5 11 3 777.6 0 3 12 33 777.6 0 33 13 5 777.6 0 5 14 6 777.6 0 6 15 4 777.6 0 4 16 3 777.6 0 3 17 8 777.6 0 8 18 4 777.6 0 4 19 3 777.6 0 3 20 8 777.6 0 8 21 6 777.6 0 6 22 4 777.6 0 4 23 3 777.6 0 3 24 1 777.6 0 1 25 2 777.6 0 2 26 6 777.6 0 6 27 4 777.6 0 4 28 10 777.6 0 10 29 6 777.6 0 6 30 5 777.6 0 5 31 14 777.6 0 14 32 5 777.6 0 5 33 12 777.6 0 12 34 11 777.6 0 11 35 7 777.6 0 7 36 12 777.6 0 12 37 50 777.6 0 50 38 14 777.6 0 14 39 19 777.6 0 19 40 15 777.6 0 15 41 4 777.6 0 4 42 7 777.6 0 7 43 9 777.6 0 9 44 7 777.6 0 7 45 7 777.6 0 7 46 8 777.6 0 8 47 11 777.6 0 11 48 8 777.6 0 8 49 9 777.6 0 9 50 14 777.6 0 14 51 41 777.6 0 41 52 35 777.6 0 35 53 4 777.6 0 4 54 16 777.6 0 16 55 9 777.6 0 9 56 200 777.6 0 200 57 124 777.6 0 124 58 80 777.6 0 80 59 97 777.6 0 97 60 3 777.6 0 3 61 17 777.6 0 17 62 19 777.6 0 19 63 16 777.6 0 16 64 37 777.6 0 37 65 14 777.6 0 14 66 12 777.6 0 12 67 43 777.6 0 43 68 12 777.6 0 12 69 55 777.6 0 55 70 103 777.6 0 103 71 53 777.6 0 53 72 13 777.6 0 13 73 16 777.6 0 16 74 16 777.6 0 16 75 40 777.6 0 40 76 57 777.6 0 57 77 28 777.6 0 28 78 32 777.6 0 32 79 58 777.6 0 58 80 56 777.6 0 56 81 36 777.6 0 36 82 55 777.6 0 55 83 65 777.6 0 65 84 56 777.6 0 56 85 66 777.6 0 66 86 72 777.6 0 72 87 69 777.6 0 69 88 139 777.6 0 139 89 1548 777.6 0 1548 90 1287 777.6 0 1287 91 1888 777.6 0 1888 92 24 777.6 0 24 93 28 777.6 0 28 94 50 777.6 0 50 95 58 777.6 0 58 96 127 777.6 0 127 97 42 777.6 0 42 98 67 777.6 0 67 99 104 777.6 0 104 100 367 777.6 0 367 101 80 777.6 0 80 102 178 777.6 0 178 103 17 777.6 0 17 104 13 777.6 0 13 105 28 777.6 0 28 106 17 777.6 0 17 107 12 777.6 0 12 108 26 777.6 0 26 109 15 777.6 0 15 110 72 777.6 0 72 111 16 777.6 0 16 112 75 777.6 0 75 113 17 777.6 0 17 114 33 777.6 0 33 115 57 777.6 0 57 116 44 777.6 0 44 117 46 777.6 0 46 118 19 777.6 0 19 119 23 777.6 0 23 120 59 777.6 0 59 121 29 777.6 0 29 122 48 777.6 0 48 123 13 777.6 0 13 124 11 777.6 0 11 125 31 777.6 0 31 126 24 777.6 0 24 127 27 777.6 0 27 128 28 777.6 0 28 129 143 777.6 0 143 130 26 777.6 0 26 131 38 777.6 0 38 132 617 777.6 0 617 133 248 777.6 0 248 134 570 777.6 0 570 135 61 777.6 0 61 136 18 777.6 0 18 137 41 777.6 0 41 138 29 777.6 0 29 139 38 777.6 0 38 140 69 777.6 0 69 141 28 777.6 0 28 142 22 777.6 0 22 143 23 777.6 0 23 144 24 777.6 0 24 145 29 777.6 0 29 146 30 777.6 0 30 147 82 777.6 0 82 148 796 777.6 0 796 149 1044 777.6 0 1044 150 494 777.6 0 494 151 18 777.6 0 18 152 12 777.6 0 12 153 20 777.6 0 20 154 16 777.6 0 16 155 33 777.6 0 33 156 31 777.6 0 31 157 73 777.6 0 73 158 38 777.6 0 38 159 30 777.6 0 30 160 54 777.6 0 54 161 20 777.6 0 20 162 32 777.6 0 32 163 19 777.6 0 19 164 36 777.6 0 36 165 80 777.6 0 80 166 87 777.6 0 87 167 92 777.6 0 92 168 599 777.6 0 599 169 188 777.6 0 188 170 98 777.6 0 98 171 20 777.6 0 20 172 25 777.6 0 25 173 50 777.6 0 50 174 61 777.6 0 61 175 117 777.6 0 117 176 2127 777.6 0 2127 177 364 777.6 0 364 178 1068 777.6 0 1068 179 33 777.6 0 33 180 36 777.6 0 36 181 31 777.6 0 31 182 22 777.6 0 22 183 30 777.6 0 30 184 67 777.6 0 67 185 69 777.6 0 69 186 68 777.6 0 68 187 20 777.6 0 20 188 9 777.6 0 9 189 11 777.6 0 11 190 15 777.6 0 15 191 20 777.6 0 20 192 28 777.6 0 28 193 94 777.6 0 94 194 64 777.6 0 64 195 29 777.6 0 29 196 33 777.6 0 33 197 24 777.6 0 24 198 45 777.6 0 45 199 49 777.6 0 49 200 34 777.6 0 34 201 19 777.6 0 19 202 35 777.6 0 35 203 59 777.6 0 59 204 53 777.6 0 53 205 86 777.6 0 86 206 68 777.6 0 68 207 248 777.6 0 248 208 292 777.6 0 292 209 103 777.6 0 103 210 94 777.6 0 94 211 49 777.6 0 49 212 55 777.6 0 55 213 70 777.6 0 70 214 68 777.6 0 68 215 99 777.6 0 99 216 154 777.6 0 154 217 131 777.6 0 131 218 198 777.6 0 198 219 330 777.6 0 330 220 5011 777.6 0 5011 221 2528 777.6 0 2528 222 2748 777.6 0 2748 223 28 777.6 0 28 224 53 777.6 0 53 225 107 777.6 0 107 226 201 777.6 0 201 227 97 777.6 0 97 228 58 777.6 0 58 229 56 777.6 0 56 230 61 777.6 0 61 231 50 777.6 0 50 232 37 777.6 0 37 233 30 777.6 0 30 234 27 777.6 0 27 235 48 777.6 0 48 236 47 777.6 0 47 237 55 777.6 0 55 238 25 777.6 0 25 239 101 777.6 0 101 240 88 777.6 0 88 241 162 777.6 0 162 242 38 777.6 0 38 243 32 777.6 0 32 244 85 777.6 0 85 245 94 777.6 0 94 246 103 777.6 0 103 247 48 777.6 0 48 248 41 777.6 0 41 249 71 777.6 0 71 250 37 777.6 0 37 251 38 777.6 0 38 252 28 777.6 0 28 253 86 777.6 0 86 254 93 777.6 0 93 255 260 777.6 0 260 256 374 777.6 0 374 257 187 777.6 0 187 258 73 777.6 0 73 259 69 777.6 0 69 260 60 777.6 0 60 261 69 777.6 0 69 262 54 777.6 0 54 263 63 777.6 0 63 264 70 777.6 0 70 265 115 777.6 0 115 266 253 777.6 0 253 267 129 777.6 0 129 268 883 777.6 0 883 269 1064 777.6 0 1064 270 870 777.6 0 870 271 423 777.6 0 423 272 216 777.6 0 216 273 157 777.6 0 157 274 860 777.6 0 860 275 523 777.6 0 523 276 368 777.6 0 368 277 59 777.6 0 59 278 115 777.6 0 115 279 265 777.6 0 265 280 490 777.6 0 490 281 1272 777.6 0 1272 282 1164 777.6 0 1164 283 18885 777.6 0 18885 284 5839 777.6 0 5839 285 9696 777.6 0 9696 286 1177 777.6 0 1177 287 52 777.6 0 52 288 146 777.6 0 146 289 662 777.6 0 662 290 1510 777.6 0 1510 291 145 777.6 0 145 292 46 777.6 0 46 293 168 777.6 0 168 294 119 777.6 0 119 295 78 777.6 0 78 296 209 777.6 0 209 297 38 777.6 0 38 298 106 777.6 0 106 299 128 777.6 0 128 300 64 777.6 0 64 301 44 777.6 0 44 Finished in 1.44 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 31,320 Reads with adapters: 7,492 (23.9%) Reads that were too short: 16 (0.1%) Reads written (passing filters): 7,476 (23.9%) Total basepairs processed: 6,823,696 bp Total written (filtered): 2,048,040 bp (30.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7492 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 64 0.0 3 0 59 3 2 17 7232 0.0 3 7011 189 28 4 18 31 0.0 3 2 23 3 3 19 33 0.0 3 0 0 25 8 20 18 0.0 3 0 0 0 18 24 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 36 0.0 3 0 0 0 36 45 3 0.0 3 3 46 14 0.0 3 0 0 0 14 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 76 1 0.0 3 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 102 6 0.0 3 0 0 0 6 105 17 0.0 3 0 0 0 17 118 1 0.0 3 0 0 0 1 124 5 0.0 3 0 0 0 5 135 4 0.0 3 0 0 0 4 138 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 1 1 185 1 0.0 3 0 0 1 202 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 7,476 Reads with adapters: 248 (3.3%) Reads written (passing filters): 7,476 (100.0%) Total basepairs processed: 2,048,040 bp Total written (filtered): 2,047,205 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 248 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.1% C: 8.1% G: 19.8% T: 37.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 168 116.8 0 168 4 69 29.2 0 69 5 11 7.3 0 11