This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 103.70 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 192,325 (6.0%) Reads that were too short: 8,977 (0.3%) Reads written (passing filters): 183,348 (5.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 38,729,368 bp (4.0%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 192325 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 121 777.6 0 121 7 2800 777.6 0 2800 8 303 777.6 0 303 9 7301 777.6 0 7301 10 27 777.6 0 27 11 21 777.6 0 21 12 9 777.6 0 9 13 16 777.6 0 16 14 15 777.6 0 15 15 7 777.6 0 7 16 12 777.6 0 12 17 5 777.6 0 5 18 14 777.6 0 14 19 9 777.6 0 9 20 7 777.6 0 7 21 10 777.6 0 10 22 8 777.6 0 8 23 12 777.6 0 12 24 10 777.6 0 10 25 6 777.6 0 6 26 9 777.6 0 9 27 6 777.6 0 6 28 11 777.6 0 11 29 13 777.6 0 13 30 16 777.6 0 16 31 9 777.6 0 9 32 9 777.6 0 9 33 10 777.6 0 10 34 16 777.6 0 16 35 24 777.6 0 24 36 59 777.6 0 59 37 12 777.6 0 12 38 15 777.6 0 15 39 10 777.6 0 10 40 107 777.6 0 107 41 11 777.6 0 11 42 18 777.6 0 18 43 15 777.6 0 15 44 17 777.6 0 17 45 9 777.6 0 9 46 12 777.6 0 12 47 18 777.6 0 18 48 16 777.6 0 16 49 16 777.6 0 16 50 8 777.6 0 8 51 28 777.6 0 28 52 20 777.6 0 20 53 28 777.6 0 28 54 135 777.6 0 135 55 51 777.6 0 51 56 9 777.6 0 9 57 16 777.6 0 16 58 11 777.6 0 11 59 10 777.6 0 10 60 16 777.6 0 16 61 31 777.6 0 31 62 19 777.6 0 19 63 51 777.6 0 51 64 117 777.6 0 117 65 94 777.6 0 94 66 50 777.6 0 50 67 60 777.6 0 60 68 51 777.6 0 51 69 77 777.6 0 77 70 62 777.6 0 62 71 67 777.6 0 67 72 37 777.6 0 37 73 29 777.6 0 29 74 45 777.6 0 45 75 65 777.6 0 65 76 152 777.6 0 152 77 283 777.6 0 283 78 835 777.6 0 835 79 1451 777.6 0 1451 80 3684 777.6 0 3684 81 86818 777.6 0 86818 82 8589 777.6 0 8589 83 18223 777.6 0 18223 84 100 777.6 0 100 85 63 777.6 0 63 86 79 777.6 0 79 87 102 777.6 0 102 88 15 777.6 0 15 89 13 777.6 0 13 90 14 777.6 0 14 91 12 777.6 0 12 92 21 777.6 0 21 93 20 777.6 0 20 94 12 777.6 0 12 95 15 777.6 0 15 96 24 777.6 0 24 97 18 777.6 0 18 98 73 777.6 0 73 99 88 777.6 0 88 100 19 777.6 0 19 101 12 777.6 0 12 102 101 777.6 0 101 103 72 777.6 0 72 104 85 777.6 0 85 105 102 777.6 0 102 106 197 777.6 0 197 107 164 777.6 0 164 108 228 777.6 0 228 109 466 777.6 0 466 110 84 777.6 0 84 111 109 777.6 0 109 112 276 777.6 0 276 113 171 777.6 0 171 114 141 777.6 0 141 115 303 777.6 0 303 116 158 777.6 0 158 117 317 777.6 0 317 118 185 777.6 0 185 119 297 777.6 0 297 120 387 777.6 0 387 121 695 777.6 0 695 122 14120 777.6 0 14120 123 11329 777.6 0 11329 124 13024 777.6 0 13024 125 99 777.6 0 99 126 79 777.6 0 79 127 24 777.6 0 24 128 26 777.6 0 26 129 75 777.6 0 75 130 54 777.6 0 54 131 74 777.6 0 74 132 22 777.6 0 22 133 26 777.6 0 26 134 40 777.6 0 40 135 45 777.6 0 45 136 31 777.6 0 31 137 39 777.6 0 39 138 40 777.6 0 40 139 45 777.6 0 45 140 37 777.6 0 37 141 34 777.6 0 34 142 43 777.6 0 43 143 121 777.6 0 121 144 626 777.6 0 626 145 403 777.6 0 403 146 295 777.6 0 295 147 48 777.6 0 48 148 21 777.6 0 21 149 17 777.6 0 17 150 27 777.6 0 27 151 53 777.6 0 53 152 146 777.6 0 146 153 57 777.6 0 57 154 35 777.6 0 35 155 62 777.6 0 62 156 42 777.6 0 42 157 25 777.6 0 25 158 42 777.6 0 42 159 56 777.6 0 56 160 31 777.6 0 31 161 20 777.6 0 20 162 30 777.6 0 30 163 30 777.6 0 30 164 38 777.6 0 38 165 29 777.6 0 29 166 37 777.6 0 37 167 26 777.6 0 26 168 42 777.6 0 42 169 32 777.6 0 32 170 56 777.6 0 56 171 176 777.6 0 176 172 71 777.6 0 71 173 43 777.6 0 43 174 14 777.6 0 14 175 26 777.6 0 26 176 27 777.6 0 27 177 29 777.6 0 29 178 56 777.6 0 56 179 63 777.6 0 63 180 48 777.6 0 48 181 69 777.6 0 69 182 1089 777.6 0 1089 183 474 777.6 0 474 184 1038 777.6 0 1038 185 42 777.6 0 42 186 12 777.6 0 12 187 15 777.6 0 15 188 25 777.6 0 25 189 86 777.6 0 86 190 206 777.6 0 206 191 18 777.6 0 18 192 16 777.6 0 16 193 17 777.6 0 17 194 23 777.6 0 23 195 44 777.6 0 44 196 70 777.6 0 70 197 83 777.6 0 83 198 20 777.6 0 20 199 19 777.6 0 19 200 19 777.6 0 19 201 26 777.6 0 26 202 38 777.6 0 38 203 41 777.6 0 41 204 26 777.6 0 26 205 49 777.6 0 49 206 44 777.6 0 44 207 83 777.6 0 83 208 20 777.6 0 20 209 26 777.6 0 26 210 35 777.6 0 35 211 38 777.6 0 38 212 36 777.6 0 36 213 16 777.6 0 16 214 38 777.6 0 38 215 39 777.6 0 39 216 39 777.6 0 39 217 30 777.6 0 30 218 25 777.6 0 25 219 12 777.6 0 12 220 26 777.6 0 26 221 46 777.6 0 46 222 82 777.6 0 82 223 48 777.6 0 48 224 33 777.6 0 33 225 40 777.6 0 40 226 32 777.6 0 32 227 20 777.6 0 20 228 22 777.6 0 22 229 21 777.6 0 21 230 11 777.6 0 11 231 9 777.6 0 9 232 21 777.6 0 21 233 53 777.6 0 53 234 74 777.6 0 74 235 15 777.6 0 15 236 24 777.6 0 24 237 29 777.6 0 29 238 27 777.6 0 27 239 47 777.6 0 47 240 867 777.6 0 867 241 241 777.6 0 241 242 437 777.6 0 437 243 30 777.6 0 30 244 21 777.6 0 21 245 28 777.6 0 28 246 13 777.6 0 13 247 15 777.6 0 15 248 8 777.6 0 8 249 34 777.6 0 34 250 46 777.6 0 46 251 72 777.6 0 72 252 49 777.6 0 49 253 36 777.6 0 36 254 43 777.6 0 43 255 40 777.6 0 40 256 19 777.6 0 19 257 22 777.6 0 22 258 15 777.6 0 15 259 50 777.6 0 50 260 48 777.6 0 48 261 246 777.6 0 246 262 188 777.6 0 188 263 136 777.6 0 136 264 169 777.6 0 169 265 157 777.6 0 157 266 94 777.6 0 94 267 26 777.6 0 26 268 47 777.6 0 47 269 44 777.6 0 44 270 100 777.6 0 100 271 41 777.6 0 41 272 38 777.6 0 38 273 34 777.6 0 34 274 52 777.6 0 52 275 118 777.6 0 118 276 83 777.6 0 83 277 45 777.6 0 45 278 45 777.6 0 45 279 23 777.6 0 23 280 71 777.6 0 71 281 38 777.6 0 38 282 62 777.6 0 62 283 252 777.6 0 252 284 28 777.6 0 28 285 49 777.6 0 49 286 43 777.6 0 43 287 52 777.6 0 52 288 127 777.6 0 127 289 65 777.6 0 65 290 323 777.6 0 323 291 1106 777.6 0 1106 292 362 777.6 0 362 293 75 777.6 0 75 294 82 777.6 0 82 295 114 777.6 0 114 296 546 777.6 0 546 297 166 777.6 0 166 298 85 777.6 0 85 299 99 777.6 0 99 300 63 777.6 0 63 301 34 777.6 0 34 Finished in 9.39 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 183,348 Reads with adapters: 10,366 (5.7%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 10,362 (5.7%) Total basepairs processed: 38,729,368 bp Total written (filtered): 2,817,968 bp (7.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10366 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 1 3 18 6 0.0 3 0 0 5 0 1 19 160 0.0 3 0 155 1 1 3 20 7334 0.0 4 7204 123 5 1 1 21 56 0.0 4 6 49 0 1 22 2780 0.0 4 2721 45 1 0 13 23 11 0.0 4 3 5 0 1 2 25 2 0.0 4 0 0 0 1 1 28 1 0.0 4 1 72 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 99 5 0.0 4 0 0 0 0 5 144 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 10,362 Reads with adapters: 194 (1.9%) Reads written (passing filters): 10,362 (100.0%) Total basepairs processed: 2,817,968 bp Total written (filtered): 2,804,408 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 194 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 3.6% G: 92.8% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 5 1 10.1 0 1 6 2 2.5 0 2 9 11 0.0 0 0 11 10 56 0.0 1 2 54 11 78 0.0 1 0 78 223 2 0.0 1 2 224 1 0.0 1 1 238 3 0.0 1 3 239 1 0.0 1 1 262 1 0.0 1 1 263 1 0.0 1 1 264 1 0.0 1 1 267 34 0.0 1 31 3 268 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.