This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 180.36 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 955,084 (17.9%) Reads that were too short: 446,565 (8.4%) Reads written (passing filters): 508,519 (9.5%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 81,095,509 bp (5.1%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 955084 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 65 1302.2 0 65 7 2792 1302.2 0 2792 8 19 1302.2 0 19 9 47 1302.2 0 47 10 38 1302.2 0 38 11 24 1302.2 0 24 12 17 1302.2 0 17 13 44 1302.2 0 44 14 25 1302.2 0 25 15 21 1302.2 0 21 16 16 1302.2 0 16 17 21 1302.2 0 21 18 23 1302.2 0 23 19 23 1302.2 0 23 20 20 1302.2 0 20 21 14 1302.2 0 14 22 28 1302.2 0 28 23 30 1302.2 0 30 24 21 1302.2 0 21 25 34 1302.2 0 34 26 47 1302.2 0 47 27 41 1302.2 0 41 28 34 1302.2 0 34 29 21 1302.2 0 21 30 29 1302.2 0 29 31 25 1302.2 0 25 32 15 1302.2 0 15 33 28 1302.2 0 28 34 24 1302.2 0 24 35 15 1302.2 0 15 36 27 1302.2 0 27 37 26 1302.2 0 26 38 21 1302.2 0 21 39 21 1302.2 0 21 40 18 1302.2 0 18 41 22 1302.2 0 22 42 31 1302.2 0 31 43 38 1302.2 0 38 44 22 1302.2 0 22 45 36 1302.2 0 36 46 40 1302.2 0 40 47 38 1302.2 0 38 48 29 1302.2 0 29 49 38 1302.2 0 38 50 25 1302.2 0 25 51 74 1302.2 0 74 52 16 1302.2 0 16 53 16 1302.2 0 16 54 21 1302.2 0 21 55 27 1302.2 0 27 56 202 1302.2 0 202 57 1323 1302.2 0 1323 58 78 1302.2 0 78 59 33 1302.2 0 33 60 32 1302.2 0 32 61 20 1302.2 0 20 62 39 1302.2 0 39 63 29 1302.2 0 29 64 29 1302.2 0 29 65 19 1302.2 0 19 66 30 1302.2 0 30 67 64 1302.2 0 64 68 71 1302.2 0 71 69 33 1302.2 0 33 70 45 1302.2 0 45 71 49 1302.2 0 49 72 985 1302.2 0 985 73 812 1302.2 0 812 74 1149 1302.2 0 1149 75 1393 1302.2 0 1393 76 3005 1302.2 0 3005 77 2675 1302.2 0 2675 78 5868 1302.2 0 5868 79 1477 1302.2 0 1477 80 1871 1302.2 0 1871 81 2656 1302.2 0 2656 82 2516 1302.2 0 2516 83 1780 1302.2 0 1780 84 860 1302.2 0 860 85 2194 1302.2 0 2194 86 730 1302.2 0 730 87 2667 1302.2 0 2667 88 3292 1302.2 0 3292 89 1408 1302.2 0 1408 90 4402 1302.2 0 4402 91 7576 1302.2 0 7576 92 647 1302.2 0 647 93 5126 1302.2 0 5126 94 5978 1302.2 0 5978 95 5144 1302.2 0 5144 96 4007 1302.2 0 4007 97 2103 1302.2 0 2103 98 1573 1302.2 0 1573 99 986 1302.2 0 986 100 922 1302.2 0 922 101 1856 1302.2 0 1856 102 2163 1302.2 0 2163 103 1442 1302.2 0 1442 104 3843 1302.2 0 3843 105 3425 1302.2 0 3425 106 2032 1302.2 0 2032 107 14305 1302.2 0 14305 108 4179 1302.2 0 4179 109 2064 1302.2 0 2064 110 1565 1302.2 0 1565 111 3265 1302.2 0 3265 112 2173 1302.2 0 2173 113 3019 1302.2 0 3019 114 3052 1302.2 0 3052 115 1224 1302.2 0 1224 116 2182 1302.2 0 2182 117 1056 1302.2 0 1056 118 1085 1302.2 0 1085 119 2608 1302.2 0 2608 120 4295 1302.2 0 4295 121 3660 1302.2 0 3660 122 4339 1302.2 0 4339 123 3855 1302.2 0 3855 124 2195 1302.2 0 2195 125 3385 1302.2 0 3385 126 4929 1302.2 0 4929 127 5140 1302.2 0 5140 128 5357 1302.2 0 5357 129 4599 1302.2 0 4599 130 2057 1302.2 0 2057 131 2137 1302.2 0 2137 132 1930 1302.2 0 1930 133 5035 1302.2 0 5035 134 7931 1302.2 0 7931 135 13449 1302.2 0 13449 136 11230 1302.2 0 11230 137 3774 1302.2 0 3774 138 2754 1302.2 0 2754 139 3571 1302.2 0 3571 140 5767 1302.2 0 5767 141 3929 1302.2 0 3929 142 4331 1302.2 0 4331 143 1975 1302.2 0 1975 144 2568 1302.2 0 2568 145 6300 1302.2 0 6300 146 5904 1302.2 0 5904 147 6441 1302.2 0 6441 148 4121 1302.2 0 4121 149 3545 1302.2 0 3545 150 3296 1302.2 0 3296 151 2459 1302.2 0 2459 152 5415 1302.2 0 5415 153 3758 1302.2 0 3758 154 5308 1302.2 0 5308 155 6683 1302.2 0 6683 156 5382 1302.2 0 5382 157 7278 1302.2 0 7278 158 6316 1302.2 0 6316 159 6005 1302.2 0 6005 160 5984 1302.2 0 5984 161 4284 1302.2 0 4284 162 3935 1302.2 0 3935 163 2817 1302.2 0 2817 164 3541 1302.2 0 3541 165 4779 1302.2 0 4779 166 5748 1302.2 0 5748 167 3891 1302.2 0 3891 168 2493 1302.2 0 2493 169 3046 1302.2 0 3046 170 2037 1302.2 0 2037 171 3251 1302.2 0 3251 172 2890 1302.2 0 2890 173 7813 1302.2 0 7813 174 9872 1302.2 0 9872 175 7764 1302.2 0 7764 176 3430 1302.2 0 3430 177 1596 1302.2 0 1596 178 1757 1302.2 0 1757 179 2062 1302.2 0 2062 180 4821 1302.2 0 4821 181 6274 1302.2 0 6274 182 5259 1302.2 0 5259 183 3339 1302.2 0 3339 184 2993 1302.2 0 2993 185 5161 1302.2 0 5161 186 7548 1302.2 0 7548 187 5128 1302.2 0 5128 188 3364 1302.2 0 3364 189 1958 1302.2 0 1958 190 3433 1302.2 0 3433 191 7601 1302.2 0 7601 192 5703 1302.2 0 5703 193 5803 1302.2 0 5803 194 3075 1302.2 0 3075 195 2382 1302.2 0 2382 196 2080 1302.2 0 2080 197 1832 1302.2 0 1832 198 3985 1302.2 0 3985 199 4741 1302.2 0 4741 200 3520 1302.2 0 3520 201 4503 1302.2 0 4503 202 3103 1302.2 0 3103 203 4186 1302.2 0 4186 204 4007 1302.2 0 4007 205 4324 1302.2 0 4324 206 3757 1302.2 0 3757 207 4222 1302.2 0 4222 208 3176 1302.2 0 3176 209 2139 1302.2 0 2139 210 4884 1302.2 0 4884 211 3899 1302.2 0 3899 212 3379 1302.2 0 3379 213 3636 1302.2 0 3636 214 3383 1302.2 0 3383 215 3980 1302.2 0 3980 216 2941 1302.2 0 2941 217 3106 1302.2 0 3106 218 5182 1302.2 0 5182 219 6139 1302.2 0 6139 220 5844 1302.2 0 5844 221 4828 1302.2 0 4828 222 2105 1302.2 0 2105 223 1726 1302.2 0 1726 224 4188 1302.2 0 4188 225 4285 1302.2 0 4285 226 4182 1302.2 0 4182 227 5005 1302.2 0 5005 228 5233 1302.2 0 5233 229 4274 1302.2 0 4274 230 3998 1302.2 0 3998 231 3582 1302.2 0 3582 232 5108 1302.2 0 5108 233 4013 1302.2 0 4013 234 2929 1302.2 0 2929 235 4444 1302.2 0 4444 236 4258 1302.2 0 4258 237 3290 1302.2 0 3290 238 2925 1302.2 0 2925 239 3503 1302.2 0 3503 240 3480 1302.2 0 3480 241 3579 1302.2 0 3579 242 3195 1302.2 0 3195 243 2559 1302.2 0 2559 244 3485 1302.2 0 3485 245 5992 1302.2 0 5992 246 6612 1302.2 0 6612 247 5314 1302.2 0 5314 248 3620 1302.2 0 3620 249 1848 1302.2 0 1848 250 1991 1302.2 0 1991 251 2799 1302.2 0 2799 252 3770 1302.2 0 3770 253 3788 1302.2 0 3788 254 4539 1302.2 0 4539 255 7488 1302.2 0 7488 256 9680 1302.2 0 9680 257 7758 1302.2 0 7758 258 5726 1302.2 0 5726 259 3580 1302.2 0 3580 260 3799 1302.2 0 3799 261 2921 1302.2 0 2921 262 2285 1302.2 0 2285 263 2461 1302.2 0 2461 264 2438 1302.2 0 2438 265 4366 1302.2 0 4366 266 7741 1302.2 0 7741 267 5512 1302.2 0 5512 268 7429 1302.2 0 7429 269 4230 1302.2 0 4230 270 3094 1302.2 0 3094 271 3714 1302.2 0 3714 272 4143 1302.2 0 4143 273 4009 1302.2 0 4009 274 4429 1302.2 0 4429 275 4873 1302.2 0 4873 276 6861 1302.2 0 6861 277 6079 1302.2 0 6079 278 4961 1302.2 0 4961 279 7714 1302.2 0 7714 280 8959 1302.2 0 8959 281 5251 1302.2 0 5251 282 3551 1302.2 0 3551 283 2794 1302.2 0 2794 284 4581 1302.2 0 4581 285 5310 1302.2 0 5310 286 9526 1302.2 0 9526 287 7549 1302.2 0 7549 288 4740 1302.2 0 4740 289 3039 1302.2 0 3039 290 2203 1302.2 0 2203 291 2049 1302.2 0 2049 292 4039 1302.2 0 4039 293 5353 1302.2 0 5353 294 6701 1302.2 0 6701 295 7302 1302.2 0 7302 296 10526 1302.2 0 10526 297 5308 1302.2 0 5308 298 4293 1302.2 0 4293 299 5097 1302.2 0 5097 300 3263 1302.2 0 3263 301 2106 1302.2 0 2106 Finished in 18.62 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 508,519 Reads with adapters: 3,030 (0.6%) Reads that were too short: 119 (0.0%) Reads written (passing filters): 2,911 (0.6%) Total basepairs processed: 81,095,509 bp Total written (filtered): 784,400 bp (1.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3030 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 10 0.0 3 0 4 1 5 17 2738 0.0 3 2295 402 37 4 18 16 0.0 3 2 3 3 8 19 10 0.0 3 2 0 1 7 20 4 0.0 3 1 0 0 3 21 2 0.0 3 0 0 0 2 22 1 0.0 3 0 0 0 1 24 3 0.0 3 0 0 0 3 26 2 0.0 3 0 0 0 2 27 4 0.0 3 0 0 0 4 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 31 2 0.0 3 0 0 0 2 32 20 0.0 3 0 0 0 20 33 2 0.0 3 0 0 0 2 35 12 0.0 3 0 0 1 11 36 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 38 5 0.0 3 0 0 0 5 39 1 0.0 3 0 0 0 1 40 4 0.0 3 0 0 0 4 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 56 2 0.0 3 0 0 0 2 57 4 0.0 3 0 0 0 4 58 14 0.0 3 0 0 2 12 59 5 0.0 3 0 0 0 5 60 3 0.0 3 0 0 0 3 61 4 0.0 3 0 0 0 4 62 3 0.0 3 0 0 0 3 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 1 1 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 78 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 6 0.0 3 0 0 0 6 85 2 0.0 3 0 0 1 1 86 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 89 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 6 0.0 3 0 0 0 6 93 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 97 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 109 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 117 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 121 3 0.0 3 0 0 0 3 123 3 0.0 3 0 0 0 3 124 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 2 0.0 3 0 0 1 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 203 3 0.0 3 0 0 0 3 206 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 2,911 Reads with adapters: 256 (8.8%) Reads written (passing filters): 2,911 (100.0%) Total basepairs processed: 784,400 bp Total written (filtered): 783,623 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 256 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.9% C: 41.8% G: 14.8% T: 21.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 250 45.5 0 250 4 5 11.4 0 5 7 1 0.2 0 1