This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 168.60 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 145,576 (2.7%) Reads that were too short: 23,673 (0.4%) Reads written (passing filters): 121,903 (2.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 30,898,769 bp (1.9%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 145576 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 807 1302.2 0 807 7 1591 1302.2 0 1591 8 83040 1302.2 0 83040 9 191 1302.2 0 191 10 5 1302.2 0 5 11 3 1302.2 0 3 12 2 1302.2 0 2 13 11 1302.2 0 11 14 8 1302.2 0 8 15 27 1302.2 0 27 16 5 1302.2 0 5 17 7 1302.2 0 7 18 14 1302.2 0 14 19 31 1302.2 0 31 20 50 1302.2 0 50 21 6 1302.2 0 6 22 8 1302.2 0 8 23 6 1302.2 0 6 24 6 1302.2 0 6 25 24 1302.2 0 24 26 23 1302.2 0 23 27 34 1302.2 0 34 28 2 1302.2 0 2 29 4 1302.2 0 4 30 4 1302.2 0 4 31 3 1302.2 0 3 32 2 1302.2 0 2 33 4 1302.2 0 4 34 4 1302.2 0 4 35 4 1302.2 0 4 36 6 1302.2 0 6 37 6 1302.2 0 6 38 1 1302.2 0 1 39 3 1302.2 0 3 40 4 1302.2 0 4 41 5 1302.2 0 5 42 1 1302.2 0 1 43 18 1302.2 0 18 44 45 1302.2 0 45 45 34 1302.2 0 34 46 9 1302.2 0 9 47 5 1302.2 0 5 48 3 1302.2 0 3 49 1 1302.2 0 1 50 5 1302.2 0 5 51 2 1302.2 0 2 52 5 1302.2 0 5 53 6 1302.2 0 6 55 4 1302.2 0 4 56 3 1302.2 0 3 57 3 1302.2 0 3 58 2 1302.2 0 2 59 9 1302.2 0 9 60 22 1302.2 0 22 61 4 1302.2 0 4 62 2 1302.2 0 2 63 4 1302.2 0 4 64 4 1302.2 0 4 65 8 1302.2 0 8 66 6 1302.2 0 6 67 10 1302.2 0 10 68 19 1302.2 0 19 69 15 1302.2 0 15 70 91 1302.2 0 91 71 118 1302.2 0 118 72 44 1302.2 0 44 73 59 1302.2 0 59 74 166 1302.2 0 166 75 132 1302.2 0 132 76 266 1302.2 0 266 77 1440 1302.2 0 1440 78 788 1302.2 0 788 79 2438 1302.2 0 2438 80 381 1302.2 0 381 81 374 1302.2 0 374 82 546 1302.2 0 546 83 799 1302.2 0 799 84 433 1302.2 0 433 85 60 1302.2 0 60 86 52 1302.2 0 52 87 38 1302.2 0 38 88 83 1302.2 0 83 89 18 1302.2 0 18 90 86 1302.2 0 86 91 312 1302.2 0 312 92 675 1302.2 0 675 93 199 1302.2 0 199 94 139 1302.2 0 139 95 83 1302.2 0 83 96 85 1302.2 0 85 97 239 1302.2 0 239 98 145 1302.2 0 145 99 42 1302.2 0 42 100 16 1302.2 0 16 101 16 1302.2 0 16 102 12 1302.2 0 12 103 19 1302.2 0 19 104 42 1302.2 0 42 105 15 1302.2 0 15 106 12 1302.2 0 12 107 46 1302.2 0 46 108 93 1302.2 0 93 109 26 1302.2 0 26 110 25 1302.2 0 25 111 26 1302.2 0 26 112 20 1302.2 0 20 113 97 1302.2 0 97 114 139 1302.2 0 139 115 171 1302.2 0 171 116 220 1302.2 0 220 117 141 1302.2 0 141 118 61 1302.2 0 61 119 45 1302.2 0 45 120 59 1302.2 0 59 121 118 1302.2 0 118 122 153 1302.2 0 153 123 255 1302.2 0 255 124 273 1302.2 0 273 125 121 1302.2 0 121 126 140 1302.2 0 140 127 90 1302.2 0 90 128 267 1302.2 0 267 129 193 1302.2 0 193 130 144 1302.2 0 144 131 59 1302.2 0 59 132 59 1302.2 0 59 133 80 1302.2 0 80 134 222 1302.2 0 222 135 600 1302.2 0 600 136 633 1302.2 0 633 137 387 1302.2 0 387 138 1148 1302.2 0 1148 139 179 1302.2 0 179 140 76 1302.2 0 76 141 104 1302.2 0 104 142 143 1302.2 0 143 143 402 1302.2 0 402 144 118 1302.2 0 118 145 77 1302.2 0 77 146 76 1302.2 0 76 147 92 1302.2 0 92 148 74 1302.2 0 74 149 54 1302.2 0 54 150 159 1302.2 0 159 151 69 1302.2 0 69 152 106 1302.2 0 106 153 63 1302.2 0 63 154 103 1302.2 0 103 155 190 1302.2 0 190 156 168 1302.2 0 168 157 89 1302.2 0 89 158 66 1302.2 0 66 159 63 1302.2 0 63 160 84 1302.2 0 84 161 97 1302.2 0 97 162 106 1302.2 0 106 163 152 1302.2 0 152 164 198 1302.2 0 198 165 139 1302.2 0 139 166 188 1302.2 0 188 167 196 1302.2 0 196 168 148 1302.2 0 148 169 80 1302.2 0 80 170 77 1302.2 0 77 171 240 1302.2 0 240 172 284 1302.2 0 284 173 242 1302.2 0 242 174 545 1302.2 0 545 175 1816 1302.2 0 1816 176 241 1302.2 0 241 177 285 1302.2 0 285 178 230 1302.2 0 230 179 159 1302.2 0 159 180 295 1302.2 0 295 181 203 1302.2 0 203 182 427 1302.2 0 427 183 218 1302.2 0 218 184 152 1302.2 0 152 185 120 1302.2 0 120 186 86 1302.2 0 86 187 134 1302.2 0 134 188 259 1302.2 0 259 189 436 1302.2 0 436 190 1368 1302.2 0 1368 191 2178 1302.2 0 2178 192 1951 1302.2 0 1951 193 1933 1302.2 0 1933 194 214 1302.2 0 214 195 150 1302.2 0 150 196 206 1302.2 0 206 197 154 1302.2 0 154 198 87 1302.2 0 87 199 178 1302.2 0 178 200 163 1302.2 0 163 201 239 1302.2 0 239 202 861 1302.2 0 861 203 370 1302.2 0 370 204 258 1302.2 0 258 205 213 1302.2 0 213 206 65 1302.2 0 65 207 76 1302.2 0 76 208 207 1302.2 0 207 209 279 1302.2 0 279 210 207 1302.2 0 207 211 202 1302.2 0 202 212 156 1302.2 0 156 213 73 1302.2 0 73 214 69 1302.2 0 69 215 112 1302.2 0 112 216 102 1302.2 0 102 217 88 1302.2 0 88 218 77 1302.2 0 77 219 55 1302.2 0 55 220 134 1302.2 0 134 221 148 1302.2 0 148 222 50 1302.2 0 50 223 40 1302.2 0 40 224 42 1302.2 0 42 225 146 1302.2 0 146 226 145 1302.2 0 145 227 151 1302.2 0 151 228 76 1302.2 0 76 229 113 1302.2 0 113 230 59 1302.2 0 59 231 58 1302.2 0 58 232 91 1302.2 0 91 233 95 1302.2 0 95 234 59 1302.2 0 59 235 45 1302.2 0 45 236 94 1302.2 0 94 237 100 1302.2 0 100 238 54 1302.2 0 54 239 44 1302.2 0 44 240 52 1302.2 0 52 241 48 1302.2 0 48 242 63 1302.2 0 63 243 34 1302.2 0 34 244 37 1302.2 0 37 245 39 1302.2 0 39 246 69 1302.2 0 69 247 102 1302.2 0 102 248 110 1302.2 0 110 249 115 1302.2 0 115 250 109 1302.2 0 109 251 84 1302.2 0 84 252 83 1302.2 0 83 253 60 1302.2 0 60 254 130 1302.2 0 130 255 146 1302.2 0 146 256 125 1302.2 0 125 257 184 1302.2 0 184 258 218 1302.2 0 218 259 536 1302.2 0 536 260 751 1302.2 0 751 261 1066 1302.2 0 1066 262 1523 1302.2 0 1523 263 683 1302.2 0 683 264 1051 1302.2 0 1051 265 178 1302.2 0 178 266 169 1302.2 0 169 267 57 1302.2 0 57 268 101 1302.2 0 101 269 128 1302.2 0 128 270 236 1302.2 0 236 271 151 1302.2 0 151 272 116 1302.2 0 116 273 78 1302.2 0 78 274 90 1302.2 0 90 275 148 1302.2 0 148 276 218 1302.2 0 218 277 267 1302.2 0 267 278 108 1302.2 0 108 279 67 1302.2 0 67 280 69 1302.2 0 69 281 196 1302.2 0 196 282 177 1302.2 0 177 283 246 1302.2 0 246 284 588 1302.2 0 588 285 1742 1302.2 0 1742 286 3049 1302.2 0 3049 287 115 1302.2 0 115 288 195 1302.2 0 195 289 730 1302.2 0 730 290 396 1302.2 0 396 291 253 1302.2 0 253 292 47 1302.2 0 47 293 33 1302.2 0 33 294 70 1302.2 0 70 295 187 1302.2 0 187 296 288 1302.2 0 288 297 354 1302.2 0 354 298 30 1302.2 0 30 299 36 1302.2 0 36 300 33 1302.2 0 33 301 95 1302.2 0 95 Finished in 5.62 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 121,903 Reads with adapters: 85,217 (69.9%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 85,199 (69.9%) Total basepairs processed: 30,898,769 bp Total written (filtered): 23,508,451 bp (76.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 85217 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 9 0.0 2 0 0 0 9 15 37 0.0 3 0 0 24 13 16 709 0.0 3 0 583 44 82 17 83182 0.0 3 80702 2181 233 66 18 402 0.0 3 37 189 142 34 19 616 0.0 3 2 0 403 211 20 213 0.0 3 1 0 1 211 21 9 0.0 3 0 0 0 9 30 5 0.0 3 1 0 0 4 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.78 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 85,199 Reads with adapters: 2,443 (2.9%) Reads written (passing filters): 85,199 (100.0%) Total basepairs processed: 23,508,451 bp Total written (filtered): 23,500,457 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2443 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.6% C: 12.0% G: 22.5% T: 24.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1839 1331.2 0 1839 4 547 332.8 0 547 5 54 83.2 0 54 6 2 20.8 0 2 7 1 5.2 0 1