This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 168.29 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 113,633 (2.1%) Reads that were too short: 8,216 (0.2%) Reads written (passing filters): 105,417 (2.0%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 28,369,670 bp (1.8%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 113633 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 761 1302.2 0 761 7 1350 1302.2 0 1350 8 3026 1302.2 0 3026 9 79657 1302.2 0 79657 10 117 1302.2 0 117 11 14 1302.2 0 14 12 12 1302.2 0 12 13 8 1302.2 0 8 14 3 1302.2 0 3 15 6 1302.2 0 6 16 14 1302.2 0 14 17 12 1302.2 0 12 18 11 1302.2 0 11 19 12 1302.2 0 12 20 10 1302.2 0 10 21 9 1302.2 0 9 22 12 1302.2 0 12 23 12 1302.2 0 12 24 18 1302.2 0 18 25 10 1302.2 0 10 26 7 1302.2 0 7 27 8 1302.2 0 8 28 13 1302.2 0 13 29 27 1302.2 0 27 30 21 1302.2 0 21 31 25 1302.2 0 25 32 132 1302.2 0 132 33 67 1302.2 0 67 34 116 1302.2 0 116 35 19 1302.2 0 19 36 24 1302.2 0 24 37 106 1302.2 0 106 38 67 1302.2 0 67 39 21 1302.2 0 21 40 22 1302.2 0 22 41 15 1302.2 0 15 42 31 1302.2 0 31 43 8 1302.2 0 8 44 9 1302.2 0 9 45 10 1302.2 0 10 46 6 1302.2 0 6 47 8 1302.2 0 8 48 7 1302.2 0 7 49 6 1302.2 0 6 50 12 1302.2 0 12 51 9 1302.2 0 9 52 98 1302.2 0 98 53 31 1302.2 0 31 54 75 1302.2 0 75 55 3 1302.2 0 3 56 11 1302.2 0 11 57 29 1302.2 0 29 58 24 1302.2 0 24 59 352 1302.2 0 352 60 2769 1302.2 0 2769 61 350 1302.2 0 350 62 152 1302.2 0 152 63 3 1302.2 0 3 64 3 1302.2 0 3 65 8 1302.2 0 8 66 9 1302.2 0 9 67 5 1302.2 0 5 68 4 1302.2 0 4 69 4 1302.2 0 4 70 8 1302.2 0 8 71 10 1302.2 0 10 72 31 1302.2 0 31 73 71 1302.2 0 71 74 3 1302.2 0 3 75 4 1302.2 0 4 76 5 1302.2 0 5 77 8 1302.2 0 8 78 3 1302.2 0 3 79 7 1302.2 0 7 80 5 1302.2 0 5 81 19 1302.2 0 19 82 11 1302.2 0 11 83 18 1302.2 0 18 84 59 1302.2 0 59 85 21 1302.2 0 21 86 38 1302.2 0 38 87 3 1302.2 0 3 88 10 1302.2 0 10 89 5 1302.2 0 5 90 19 1302.2 0 19 91 11 1302.2 0 11 92 19 1302.2 0 19 93 61 1302.2 0 61 94 62 1302.2 0 62 95 89 1302.2 0 89 96 16 1302.2 0 16 97 25 1302.2 0 25 98 8 1302.2 0 8 99 11 1302.2 0 11 100 10 1302.2 0 10 101 12 1302.2 0 12 102 15 1302.2 0 15 103 25 1302.2 0 25 104 31 1302.2 0 31 105 16 1302.2 0 16 106 14 1302.2 0 14 107 15 1302.2 0 15 108 65 1302.2 0 65 109 27 1302.2 0 27 110 37 1302.2 0 37 111 17 1302.2 0 17 112 42 1302.2 0 42 113 16 1302.2 0 16 114 8 1302.2 0 8 115 24 1302.2 0 24 116 13 1302.2 0 13 117 56 1302.2 0 56 118 36 1302.2 0 36 119 49 1302.2 0 49 120 56 1302.2 0 56 121 34 1302.2 0 34 122 40 1302.2 0 40 123 35 1302.2 0 35 124 66 1302.2 0 66 125 40 1302.2 0 40 126 45 1302.2 0 45 127 262 1302.2 0 262 128 54 1302.2 0 54 129 87 1302.2 0 87 130 30 1302.2 0 30 131 63 1302.2 0 63 132 73 1302.2 0 73 133 80 1302.2 0 80 134 51 1302.2 0 51 135 36 1302.2 0 36 136 44 1302.2 0 44 137 46 1302.2 0 46 138 41 1302.2 0 41 139 82 1302.2 0 82 140 101 1302.2 0 101 141 93 1302.2 0 93 142 145 1302.2 0 145 143 272 1302.2 0 272 144 4558 1302.2 0 4558 145 1029 1302.2 0 1029 146 2311 1302.2 0 2311 147 30 1302.2 0 30 148 56 1302.2 0 56 149 11 1302.2 0 11 150 14 1302.2 0 14 151 126 1302.2 0 126 152 70 1302.2 0 70 153 71 1302.2 0 71 154 33 1302.2 0 33 155 44 1302.2 0 44 156 93 1302.2 0 93 157 175 1302.2 0 175 158 59 1302.2 0 59 159 88 1302.2 0 88 160 27 1302.2 0 27 161 48 1302.2 0 48 162 25 1302.2 0 25 163 40 1302.2 0 40 164 42 1302.2 0 42 165 33 1302.2 0 33 166 67 1302.2 0 67 167 53 1302.2 0 53 168 26 1302.2 0 26 169 30 1302.2 0 30 170 87 1302.2 0 87 171 113 1302.2 0 113 172 252 1302.2 0 252 173 197 1302.2 0 197 174 41 1302.2 0 41 175 29 1302.2 0 29 176 44 1302.2 0 44 177 108 1302.2 0 108 178 127 1302.2 0 127 179 165 1302.2 0 165 180 72 1302.2 0 72 181 91 1302.2 0 91 182 51 1302.2 0 51 183 55 1302.2 0 55 184 47 1302.2 0 47 185 106 1302.2 0 106 186 40 1302.2 0 40 187 26 1302.2 0 26 188 17 1302.2 0 17 189 40 1302.2 0 40 190 461 1302.2 0 461 191 96 1302.2 0 96 192 141 1302.2 0 141 193 36 1302.2 0 36 194 47 1302.2 0 47 195 45 1302.2 0 45 196 125 1302.2 0 125 197 284 1302.2 0 284 198 299 1302.2 0 299 199 76 1302.2 0 76 200 36 1302.2 0 36 201 40 1302.2 0 40 202 18 1302.2 0 18 203 23 1302.2 0 23 204 25 1302.2 0 25 205 64 1302.2 0 64 206 52 1302.2 0 52 207 250 1302.2 0 250 208 56 1302.2 0 56 209 24 1302.2 0 24 210 14 1302.2 0 14 211 26 1302.2 0 26 212 44 1302.2 0 44 213 63 1302.2 0 63 214 46 1302.2 0 46 215 39 1302.2 0 39 216 39 1302.2 0 39 217 350 1302.2 0 350 218 62 1302.2 0 62 219 448 1302.2 0 448 220 64 1302.2 0 64 221 23 1302.2 0 23 222 27 1302.2 0 27 223 24 1302.2 0 24 224 21 1302.2 0 21 225 65 1302.2 0 65 226 87 1302.2 0 87 227 93 1302.2 0 93 228 60 1302.2 0 60 229 78 1302.2 0 78 230 33 1302.2 0 33 231 32 1302.2 0 32 232 26 1302.2 0 26 233 20 1302.2 0 20 234 20 1302.2 0 20 235 32 1302.2 0 32 236 284 1302.2 0 284 237 179 1302.2 0 179 238 115 1302.2 0 115 239 17 1302.2 0 17 240 46 1302.2 0 46 241 42 1302.2 0 42 242 28 1302.2 0 28 243 9 1302.2 0 9 244 13 1302.2 0 13 245 30 1302.2 0 30 246 67 1302.2 0 67 247 88 1302.2 0 88 248 96 1302.2 0 96 249 29 1302.2 0 29 250 9 1302.2 0 9 251 14 1302.2 0 14 252 44 1302.2 0 44 253 39 1302.2 0 39 254 78 1302.2 0 78 255 113 1302.2 0 113 256 138 1302.2 0 138 257 33 1302.2 0 33 258 14 1302.2 0 14 259 20 1302.2 0 20 260 155 1302.2 0 155 261 304 1302.2 0 304 262 260 1302.2 0 260 263 186 1302.2 0 186 264 79 1302.2 0 79 265 116 1302.2 0 116 266 82 1302.2 0 82 267 99 1302.2 0 99 268 30 1302.2 0 30 269 26 1302.2 0 26 270 64 1302.2 0 64 271 82 1302.2 0 82 272 69 1302.2 0 69 273 17 1302.2 0 17 274 30 1302.2 0 30 275 15 1302.2 0 15 276 22 1302.2 0 22 277 48 1302.2 0 48 278 49 1302.2 0 49 279 32 1302.2 0 32 280 31 1302.2 0 31 281 33 1302.2 0 33 282 67 1302.2 0 67 283 453 1302.2 0 453 284 196 1302.2 0 196 285 1212 1302.2 0 1212 286 67 1302.2 0 67 287 41 1302.2 0 41 288 48 1302.2 0 48 289 17 1302.2 0 17 290 47 1302.2 0 47 291 69 1302.2 0 69 292 77 1302.2 0 77 293 106 1302.2 0 106 294 26 1302.2 0 26 295 20 1302.2 0 20 296 8 1302.2 0 8 297 4 1302.2 0 4 298 4 1302.2 0 4 299 6 1302.2 0 6 300 8 1302.2 0 8 301 18 1302.2 0 18 Finished in 5.24 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 105,417 Reads with adapters: 84,503 (80.2%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 84,498 (80.2%) Total basepairs processed: 28,369,670 bp Total written (filtered): 22,990,825 bp (81.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 84503 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 5 3 18 41 0.0 3 0 0 40 1 19 1355 0.0 3 0 1317 22 0 16 20 82831 0.0 4 81582 1159 44 13 33 21 180 0.0 4 37 124 2 14 3 22 42 0.0 4 0 0 20 1 21 23 28 0.0 4 2 0 0 21 5 24 3 0.0 4 0 0 0 2 1 25 2 0.0 4 1 0 0 0 1 27 1 0.0 4 0 0 0 1 37 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 0 2 120 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.55 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 84,498 Reads with adapters: 7,523 (8.9%) Reads written (passing filters): 84,498 (100.0%) Total basepairs processed: 22,990,825 bp Total written (filtered): 21,388,703 bp (93.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7523 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 81.3% C: 1.3% G: 17.2% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 1320.3 0 12 4 6 330.1 0 6 5 1 82.5 0 1 6 62 20.6 0 62 7 1 5.2 0 1 8 3 1.3 0 3 9 188 0.3 0 8 180 10 432 0.1 1 15 417 11 634 0.0 1 4 630 12 2 0.0 1 0 2 13 1 0.0 1 0 1 150 4 0.0 1 1 3 223 1 0.0 1 1 224 1 0.0 1 1 233 1 0.0 1 1 243 1 0.0 1 1 254 2 0.0 1 1 1 256 19 0.0 1 18 1 257 5701 0.0 1 5182 519 258 306 0.0 1 286 20 259 86 0.0 1 81 5 260 56 0.0 1 48 8 261 1 0.0 1 1 265 1 0.0 1 1 271 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.