This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 177.03 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 166,675 (3.1%) Reads that were too short: 75,010 (1.4%) Reads written (passing filters): 91,665 (1.7%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 17,561,870 bp (1.1%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 166675 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 265 1302.2 0 265 7 21036 1302.2 0 21036 8 80 1302.2 0 80 9 10 1302.2 0 10 10 7 1302.2 0 7 11 3 1302.2 0 3 12 4 1302.2 0 4 13 4 1302.2 0 4 14 13 1302.2 0 13 15 14 1302.2 0 14 16 8 1302.2 0 8 17 4 1302.2 0 4 18 39 1302.2 0 39 19 13 1302.2 0 13 20 20 1302.2 0 20 21 5 1302.2 0 5 22 2 1302.2 0 2 23 6 1302.2 0 6 24 3 1302.2 0 3 25 4 1302.2 0 4 26 10 1302.2 0 10 27 15 1302.2 0 15 28 27 1302.2 0 27 29 11 1302.2 0 11 30 17 1302.2 0 17 31 11 1302.2 0 11 32 7 1302.2 0 7 33 6 1302.2 0 6 34 12 1302.2 0 12 35 13 1302.2 0 13 36 18 1302.2 0 18 37 69 1302.2 0 69 38 59 1302.2 0 59 39 8 1302.2 0 8 40 13 1302.2 0 13 41 11 1302.2 0 11 42 21 1302.2 0 21 43 121 1302.2 0 121 44 96 1302.2 0 96 45 184 1302.2 0 184 46 4 1302.2 0 4 47 12 1302.2 0 12 48 5 1302.2 0 5 49 15 1302.2 0 15 50 13 1302.2 0 13 51 6 1302.2 0 6 52 6 1302.2 0 6 53 8 1302.2 0 8 54 10 1302.2 0 10 55 8 1302.2 0 8 56 14 1302.2 0 14 57 145 1302.2 0 145 58 19 1302.2 0 19 59 7 1302.2 0 7 60 4 1302.2 0 4 61 11 1302.2 0 11 62 4 1302.2 0 4 63 3 1302.2 0 3 64 4 1302.2 0 4 65 4 1302.2 0 4 66 4 1302.2 0 4 67 6 1302.2 0 6 68 6 1302.2 0 6 69 4 1302.2 0 4 70 4 1302.2 0 4 71 20 1302.2 0 20 72 16 1302.2 0 16 73 54 1302.2 0 54 74 59 1302.2 0 59 75 401 1302.2 0 401 76 246 1302.2 0 246 77 198 1302.2 0 198 78 330 1302.2 0 330 79 870 1302.2 0 870 80 635 1302.2 0 635 81 311 1302.2 0 311 82 992 1302.2 0 992 83 1010 1302.2 0 1010 84 295 1302.2 0 295 85 151 1302.2 0 151 86 128 1302.2 0 128 87 198 1302.2 0 198 88 208 1302.2 0 208 89 309 1302.2 0 309 90 187 1302.2 0 187 91 1351 1302.2 0 1351 92 462 1302.2 0 462 93 523 1302.2 0 523 94 1019 1302.2 0 1019 95 367 1302.2 0 367 96 314 1302.2 0 314 97 599 1302.2 0 599 98 305 1302.2 0 305 99 415 1302.2 0 415 100 120 1302.2 0 120 101 107 1302.2 0 107 102 39 1302.2 0 39 103 48 1302.2 0 48 104 91 1302.2 0 91 105 65 1302.2 0 65 106 67 1302.2 0 67 107 114 1302.2 0 114 108 255 1302.2 0 255 109 280 1302.2 0 280 110 189 1302.2 0 189 111 212 1302.2 0 212 112 201 1302.2 0 201 113 343 1302.2 0 343 114 320 1302.2 0 320 115 330 1302.2 0 330 116 432 1302.2 0 432 117 498 1302.2 0 498 118 197 1302.2 0 197 119 193 1302.2 0 193 120 787 1302.2 0 787 121 611 1302.2 0 611 122 658 1302.2 0 658 123 503 1302.2 0 503 124 288 1302.2 0 288 125 472 1302.2 0 472 126 386 1302.2 0 386 127 437 1302.2 0 437 128 646 1302.2 0 646 129 1045 1302.2 0 1045 130 1022 1302.2 0 1022 131 499 1302.2 0 499 132 502 1302.2 0 502 133 513 1302.2 0 513 134 744 1302.2 0 744 135 3909 1302.2 0 3909 136 2413 1302.2 0 2413 137 2538 1302.2 0 2538 138 546 1302.2 0 546 139 463 1302.2 0 463 140 706 1302.2 0 706 141 552 1302.2 0 552 142 603 1302.2 0 603 143 594 1302.2 0 594 144 616 1302.2 0 616 145 621 1302.2 0 621 146 613 1302.2 0 613 147 443 1302.2 0 443 148 372 1302.2 0 372 149 366 1302.2 0 366 150 480 1302.2 0 480 151 349 1302.2 0 349 152 292 1302.2 0 292 153 316 1302.2 0 316 154 546 1302.2 0 546 155 746 1302.2 0 746 156 863 1302.2 0 863 157 473 1302.2 0 473 158 495 1302.2 0 495 159 428 1302.2 0 428 160 438 1302.2 0 438 161 534 1302.2 0 534 162 419 1302.2 0 419 163 532 1302.2 0 532 164 579 1302.2 0 579 165 754 1302.2 0 754 166 945 1302.2 0 945 167 935 1302.2 0 935 168 789 1302.2 0 789 169 290 1302.2 0 290 170 224 1302.2 0 224 171 220 1302.2 0 220 172 436 1302.2 0 436 173 398 1302.2 0 398 174 522 1302.2 0 522 175 307 1302.2 0 307 176 317 1302.2 0 317 177 293 1302.2 0 293 178 484 1302.2 0 484 179 603 1302.2 0 603 180 850 1302.2 0 850 181 830 1302.2 0 830 182 718 1302.2 0 718 183 651 1302.2 0 651 184 687 1302.2 0 687 185 551 1302.2 0 551 186 465 1302.2 0 465 187 211 1302.2 0 211 188 307 1302.2 0 307 189 478 1302.2 0 478 190 777 1302.2 0 777 191 876 1302.2 0 876 192 846 1302.2 0 846 193 519 1302.2 0 519 194 406 1302.2 0 406 195 420 1302.2 0 420 196 1535 1302.2 0 1535 197 816 1302.2 0 816 198 279 1302.2 0 279 199 142 1302.2 0 142 200 244 1302.2 0 244 201 913 1302.2 0 913 202 2413 1302.2 0 2413 203 1833 1302.2 0 1833 204 1338 1302.2 0 1338 205 475 1302.2 0 475 206 283 1302.2 0 283 207 470 1302.2 0 470 208 1210 1302.2 0 1210 209 1401 1302.2 0 1401 210 1214 1302.2 0 1214 211 1587 1302.2 0 1587 212 1295 1302.2 0 1295 213 1180 1302.2 0 1180 214 694 1302.2 0 694 215 1244 1302.2 0 1244 216 1316 1302.2 0 1316 217 910 1302.2 0 910 218 528 1302.2 0 528 219 293 1302.2 0 293 220 450 1302.2 0 450 221 940 1302.2 0 940 222 1760 1302.2 0 1760 223 740 1302.2 0 740 224 865 1302.2 0 865 225 978 1302.2 0 978 226 763 1302.2 0 763 227 551 1302.2 0 551 228 612 1302.2 0 612 229 459 1302.2 0 459 230 319 1302.2 0 319 231 285 1302.2 0 285 232 638 1302.2 0 638 233 704 1302.2 0 704 234 743 1302.2 0 743 235 956 1302.2 0 956 236 905 1302.2 0 905 237 465 1302.2 0 465 238 301 1302.2 0 301 239 247 1302.2 0 247 240 340 1302.2 0 340 241 663 1302.2 0 663 242 901 1302.2 0 901 243 1471 1302.2 0 1471 244 1144 1302.2 0 1144 245 647 1302.2 0 647 246 397 1302.2 0 397 247 321 1302.2 0 321 248 238 1302.2 0 238 249 344 1302.2 0 344 250 549 1302.2 0 549 251 903 1302.2 0 903 252 697 1302.2 0 697 253 516 1302.2 0 516 254 476 1302.2 0 476 255 641 1302.2 0 641 256 523 1302.2 0 523 257 305 1302.2 0 305 258 298 1302.2 0 298 259 352 1302.2 0 352 260 308 1302.2 0 308 261 341 1302.2 0 341 262 392 1302.2 0 392 263 566 1302.2 0 566 264 932 1302.2 0 932 265 1108 1302.2 0 1108 266 1604 1302.2 0 1604 267 499 1302.2 0 499 268 268 1302.2 0 268 269 596 1302.2 0 596 270 673 1302.2 0 673 271 1115 1302.2 0 1115 272 822 1302.2 0 822 273 659 1302.2 0 659 274 562 1302.2 0 562 275 628 1302.2 0 628 276 544 1302.2 0 544 277 630 1302.2 0 630 278 739 1302.2 0 739 279 607 1302.2 0 607 280 562 1302.2 0 562 281 462 1302.2 0 462 282 429 1302.2 0 429 283 509 1302.2 0 509 284 574 1302.2 0 574 285 431 1302.2 0 431 286 808 1302.2 0 808 287 1163 1302.2 0 1163 288 732 1302.2 0 732 289 486 1302.2 0 486 290 660 1302.2 0 660 291 686 1302.2 0 686 292 722 1302.2 0 722 293 478 1302.2 0 478 294 520 1302.2 0 520 295 752 1302.2 0 752 296 992 1302.2 0 992 297 794 1302.2 0 794 298 1541 1302.2 0 1541 299 1914 1302.2 0 1914 300 1031 1302.2 0 1031 301 80 1302.2 0 80 Finished in 3.86 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 91,665 Reads with adapters: 21,207 (23.1%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 21,185 (23.1%) Total basepairs processed: 17,561,870 bp Total written (filtered): 5,863,117 bp (33.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 21207 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 5 0.0 3 0 0 5 16 197 0.0 3 0 173 10 14 17 20797 0.0 3 20055 652 69 21 18 88 0.0 3 7 38 37 6 19 49 0.0 3 0 0 27 22 20 14 0.0 3 0 0 0 14 25 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 1 44 3 0.0 3 0 0 0 3 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 52 5 0.0 3 0 0 0 5 53 2 0.0 3 0 0 0 2 55 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.98 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 21,185 Reads with adapters: 2,260 (10.7%) Reads written (passing filters): 21,185 (100.0%) Total basepairs processed: 5,863,117 bp Total written (filtered): 5,856,303 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2260 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.3% C: 60.8% G: 5.4% T: 15.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2232 331.0 0 2232 4 24 82.8 0 24 5 2 20.7 0 2 6 2 5.2 0 2