This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 177.62 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 74,076 (1.4%) Reads that were too short: 20,851 (0.4%) Reads written (passing filters): 53,225 (1.0%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 11,833,376 bp (0.7%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 74076 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 166 1302.2 0 166 7 656 1302.2 0 656 8 20733 1302.2 0 20733 9 37 1302.2 0 37 10 14 1302.2 0 14 11 11 1302.2 0 11 12 13 1302.2 0 13 13 16 1302.2 0 16 14 9 1302.2 0 9 15 9 1302.2 0 9 16 9 1302.2 0 9 17 145 1302.2 0 145 18 54 1302.2 0 54 19 9 1302.2 0 9 20 24 1302.2 0 24 21 13 1302.2 0 13 22 16 1302.2 0 16 23 10 1302.2 0 10 24 8 1302.2 0 8 25 11 1302.2 0 11 26 11 1302.2 0 11 27 6 1302.2 0 6 28 14 1302.2 0 14 29 15 1302.2 0 15 30 11 1302.2 0 11 31 14 1302.2 0 14 32 11 1302.2 0 11 33 12 1302.2 0 12 34 27 1302.2 0 27 35 14 1302.2 0 14 36 10 1302.2 0 10 37 62 1302.2 0 62 38 174 1302.2 0 174 39 23 1302.2 0 23 40 11 1302.2 0 11 41 8 1302.2 0 8 42 10 1302.2 0 10 43 12 1302.2 0 12 44 21 1302.2 0 21 45 15 1302.2 0 15 46 14 1302.2 0 14 47 13 1302.2 0 13 48 18 1302.2 0 18 49 11 1302.2 0 11 50 5 1302.2 0 5 51 12 1302.2 0 12 52 9 1302.2 0 9 53 19 1302.2 0 19 54 28 1302.2 0 28 55 8 1302.2 0 8 56 14 1302.2 0 14 57 15 1302.2 0 15 58 17 1302.2 0 17 59 33 1302.2 0 33 60 27 1302.2 0 27 61 28 1302.2 0 28 62 7 1302.2 0 7 63 17 1302.2 0 17 64 24 1302.2 0 24 65 25 1302.2 0 25 66 31 1302.2 0 31 67 58 1302.2 0 58 68 53 1302.2 0 53 69 88 1302.2 0 88 70 203 1302.2 0 203 71 1938 1302.2 0 1938 72 1028 1302.2 0 1028 73 999 1302.2 0 999 74 19 1302.2 0 19 75 12 1302.2 0 12 76 12 1302.2 0 12 77 57 1302.2 0 57 78 217 1302.2 0 217 79 248 1302.2 0 248 80 48 1302.2 0 48 81 39 1302.2 0 39 82 32 1302.2 0 32 83 35 1302.2 0 35 84 31 1302.2 0 31 85 42 1302.2 0 42 86 18 1302.2 0 18 87 54 1302.2 0 54 88 50 1302.2 0 50 89 31 1302.2 0 31 90 8 1302.2 0 8 91 69 1302.2 0 69 92 110 1302.2 0 110 93 1102 1302.2 0 1102 94 469 1302.2 0 469 95 528 1302.2 0 528 96 560 1302.2 0 560 97 299 1302.2 0 299 98 3078 1302.2 0 3078 99 373 1302.2 0 373 100 120 1302.2 0 120 101 88 1302.2 0 88 102 31 1302.2 0 31 103 20 1302.2 0 20 104 31 1302.2 0 31 105 28 1302.2 0 28 106 20 1302.2 0 20 107 26 1302.2 0 26 108 65 1302.2 0 65 109 44 1302.2 0 44 110 26 1302.2 0 26 111 35 1302.2 0 35 112 34 1302.2 0 34 113 30 1302.2 0 30 114 73 1302.2 0 73 115 140 1302.2 0 140 116 178 1302.2 0 178 117 202 1302.2 0 202 118 122 1302.2 0 122 119 93 1302.2 0 93 120 114 1302.2 0 114 121 88 1302.2 0 88 122 113 1302.2 0 113 123 143 1302.2 0 143 124 310 1302.2 0 310 125 649 1302.2 0 649 126 134 1302.2 0 134 127 113 1302.2 0 113 128 123 1302.2 0 123 129 414 1302.2 0 414 130 328 1302.2 0 328 131 181 1302.2 0 181 132 309 1302.2 0 309 133 132 1302.2 0 132 134 211 1302.2 0 211 135 324 1302.2 0 324 136 335 1302.2 0 335 137 260 1302.2 0 260 138 178 1302.2 0 178 139 110 1302.2 0 110 140 68 1302.2 0 68 141 68 1302.2 0 68 142 78 1302.2 0 78 143 148 1302.2 0 148 144 220 1302.2 0 220 145 207 1302.2 0 207 146 144 1302.2 0 144 147 44 1302.2 0 44 148 39 1302.2 0 39 149 34 1302.2 0 34 150 62 1302.2 0 62 151 66 1302.2 0 66 152 69 1302.2 0 69 153 120 1302.2 0 120 154 194 1302.2 0 194 155 409 1302.2 0 409 156 312 1302.2 0 312 157 171 1302.2 0 171 158 194 1302.2 0 194 159 136 1302.2 0 136 160 98 1302.2 0 98 161 103 1302.2 0 103 162 112 1302.2 0 112 163 227 1302.2 0 227 164 245 1302.2 0 245 165 376 1302.2 0 376 166 223 1302.2 0 223 167 223 1302.2 0 223 168 199 1302.2 0 199 169 100 1302.2 0 100 170 102 1302.2 0 102 171 172 1302.2 0 172 172 323 1302.2 0 323 173 234 1302.2 0 234 174 227 1302.2 0 227 175 134 1302.2 0 134 176 99 1302.2 0 99 177 116 1302.2 0 116 178 207 1302.2 0 207 179 280 1302.2 0 280 180 312 1302.2 0 312 181 250 1302.2 0 250 182 175 1302.2 0 175 183 192 1302.2 0 192 184 207 1302.2 0 207 185 170 1302.2 0 170 186 84 1302.2 0 84 187 51 1302.2 0 51 188 53 1302.2 0 53 189 128 1302.2 0 128 190 620 1302.2 0 620 191 658 1302.2 0 658 192 1378 1302.2 0 1378 193 449 1302.2 0 449 194 349 1302.2 0 349 195 184 1302.2 0 184 196 182 1302.2 0 182 197 134 1302.2 0 134 198 96 1302.2 0 96 199 83 1302.2 0 83 200 84 1302.2 0 84 201 101 1302.2 0 101 202 201 1302.2 0 201 203 386 1302.2 0 386 204 607 1302.2 0 607 205 222 1302.2 0 222 206 201 1302.2 0 201 207 86 1302.2 0 86 208 102 1302.2 0 102 209 131 1302.2 0 131 210 363 1302.2 0 363 211 662 1302.2 0 662 212 525 1302.2 0 525 213 233 1302.2 0 233 214 148 1302.2 0 148 215 238 1302.2 0 238 216 312 1302.2 0 312 217 298 1302.2 0 298 218 214 1302.2 0 214 219 110 1302.2 0 110 220 153 1302.2 0 153 221 247 1302.2 0 247 222 411 1302.2 0 411 223 194 1302.2 0 194 224 289 1302.2 0 289 225 319 1302.2 0 319 226 355 1302.2 0 355 227 336 1302.2 0 336 228 207 1302.2 0 207 229 141 1302.2 0 141 230 67 1302.2 0 67 231 59 1302.2 0 59 232 93 1302.2 0 93 233 187 1302.2 0 187 234 159 1302.2 0 159 235 197 1302.2 0 197 236 239 1302.2 0 239 237 199 1302.2 0 199 238 104 1302.2 0 104 239 60 1302.2 0 60 240 52 1302.2 0 52 241 93 1302.2 0 93 242 158 1302.2 0 158 243 199 1302.2 0 199 244 170 1302.2 0 170 245 194 1302.2 0 194 246 96 1302.2 0 96 247 56 1302.2 0 56 248 38 1302.2 0 38 249 76 1302.2 0 76 250 115 1302.2 0 115 251 250 1302.2 0 250 252 172 1302.2 0 172 253 270 1302.2 0 270 254 214 1302.2 0 214 255 305 1302.2 0 305 256 191 1302.2 0 191 257 133 1302.2 0 133 258 99 1302.2 0 99 259 103 1302.2 0 103 260 149 1302.2 0 149 261 386 1302.2 0 386 262 303 1302.2 0 303 263 279 1302.2 0 279 264 366 1302.2 0 366 265 391 1302.2 0 391 266 294 1302.2 0 294 267 103 1302.2 0 103 268 45 1302.2 0 45 269 47 1302.2 0 47 270 115 1302.2 0 115 271 296 1302.2 0 296 272 445 1302.2 0 445 273 251 1302.2 0 251 274 114 1302.2 0 114 275 129 1302.2 0 129 276 91 1302.2 0 91 277 190 1302.2 0 190 278 325 1302.2 0 325 279 170 1302.2 0 170 280 112 1302.2 0 112 281 98 1302.2 0 98 282 121 1302.2 0 121 283 143 1302.2 0 143 284 301 1302.2 0 301 285 347 1302.2 0 347 286 225 1302.2 0 225 287 92 1302.2 0 92 288 62 1302.2 0 62 289 114 1302.2 0 114 290 290 1302.2 0 290 291 465 1302.2 0 465 292 237 1302.2 0 237 293 146 1302.2 0 146 294 55 1302.2 0 55 295 64 1302.2 0 64 296 87 1302.2 0 87 297 264 1302.2 0 264 298 294 1302.2 0 294 299 321 1302.2 0 321 300 317 1302.2 0 317 301 168 1302.2 0 168 Finished in 2.63 s (49 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 53,225 Reads with adapters: 21,318 (40.1%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 21,308 (40.0%) Total basepairs processed: 11,833,376 bp Total written (filtered): 5,817,188 bp (49.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21318 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 14 0.0 3 0 0 0 0 14 18 15 0.0 3 0 0 12 1 2 19 395 0.0 3 0 384 4 1 6 20 20809 0.0 4 20459 330 11 7 2 21 46 0.0 4 9 35 0 1 1 22 8 0.0 4 0 0 1 0 7 23 6 0.0 4 0 0 0 6 24 5 0.0 4 0 0 0 0 5 29 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 1 112 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 21,308 Reads with adapters: 591 (2.8%) Reads written (passing filters): 21,308 (100.0%) Total basepairs processed: 5,817,188 bp Total written (filtered): 5,808,675 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 591 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 1.9% G: 94.6% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 332.9 0 2 4 74 83.2 0 74 5 1 20.8 0 1 10 40 0.0 1 3 37 11 461 0.0 1 0 461 12 1 0.0 1 0 1 105 1 0.0 1 0 1 151 1 0.0 1 0 1 202 2 0.0 1 2 224 1 0.0 1 1 259 5 0.0 1 5 272 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.