This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 166.26 s (31 us/read; 1.92 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 82,953 (1.6%) Reads that were too short: 12,879 (0.2%) Reads written (passing filters): 70,074 (1.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 16,570,502 bp (1.0%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 82953 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 878 1302.2 0 878 7 605 1302.2 0 605 8 760 1302.2 0 760 9 41534 1302.2 0 41534 10 58 1302.2 0 58 11 37 1302.2 0 37 12 21 1302.2 0 21 13 27 1302.2 0 27 14 51 1302.2 0 51 15 28 1302.2 0 28 16 26 1302.2 0 26 17 32 1302.2 0 32 18 55 1302.2 0 55 19 28 1302.2 0 28 20 24 1302.2 0 24 21 39 1302.2 0 39 22 31 1302.2 0 31 23 32 1302.2 0 32 24 19 1302.2 0 19 25 18 1302.2 0 18 26 34 1302.2 0 34 27 26 1302.2 0 26 28 30 1302.2 0 30 29 26 1302.2 0 26 30 30 1302.2 0 30 31 38 1302.2 0 38 32 25 1302.2 0 25 33 21 1302.2 0 21 34 22 1302.2 0 22 35 15 1302.2 0 15 36 17 1302.2 0 17 37 27 1302.2 0 27 38 33 1302.2 0 33 39 41 1302.2 0 41 40 35 1302.2 0 35 41 43 1302.2 0 43 42 69 1302.2 0 69 43 63 1302.2 0 63 44 116 1302.2 0 116 45 27 1302.2 0 27 46 25 1302.2 0 25 47 17 1302.2 0 17 48 36 1302.2 0 36 49 16 1302.2 0 16 50 164 1302.2 0 164 51 37 1302.2 0 37 52 45 1302.2 0 45 53 28 1302.2 0 28 54 33 1302.2 0 33 55 24 1302.2 0 24 56 19 1302.2 0 19 57 19 1302.2 0 19 58 27 1302.2 0 27 59 20 1302.2 0 20 60 8 1302.2 0 8 61 17 1302.2 0 17 62 25 1302.2 0 25 63 21 1302.2 0 21 64 11 1302.2 0 11 65 27 1302.2 0 27 66 44 1302.2 0 44 67 23 1302.2 0 23 68 21 1302.2 0 21 69 22 1302.2 0 22 70 20 1302.2 0 20 71 16 1302.2 0 16 72 20 1302.2 0 20 73 93 1302.2 0 93 74 116 1302.2 0 116 75 172 1302.2 0 172 76 25 1302.2 0 25 77 36 1302.2 0 36 78 16 1302.2 0 16 79 60 1302.2 0 60 80 69 1302.2 0 69 81 268 1302.2 0 268 82 58 1302.2 0 58 83 49 1302.2 0 49 84 145 1302.2 0 145 85 48 1302.2 0 48 86 370 1302.2 0 370 87 96 1302.2 0 96 88 48 1302.2 0 48 89 42 1302.2 0 42 90 51 1302.2 0 51 91 77 1302.2 0 77 92 410 1302.2 0 410 93 164 1302.2 0 164 94 398 1302.2 0 398 95 114 1302.2 0 114 96 263 1302.2 0 263 97 63 1302.2 0 63 98 38 1302.2 0 38 99 21 1302.2 0 21 100 30 1302.2 0 30 101 36 1302.2 0 36 102 18 1302.2 0 18 103 25 1302.2 0 25 104 17 1302.2 0 17 105 16 1302.2 0 16 106 30 1302.2 0 30 107 20 1302.2 0 20 108 40 1302.2 0 40 109 18 1302.2 0 18 110 21 1302.2 0 21 111 22 1302.2 0 22 112 33 1302.2 0 33 113 106 1302.2 0 106 114 52 1302.2 0 52 115 46 1302.2 0 46 116 18 1302.2 0 18 117 53 1302.2 0 53 118 31 1302.2 0 31 119 118 1302.2 0 118 120 111 1302.2 0 111 121 91 1302.2 0 91 122 97 1302.2 0 97 123 97 1302.2 0 97 124 489 1302.2 0 489 125 131 1302.2 0 131 126 83 1302.2 0 83 127 121 1302.2 0 121 128 68 1302.2 0 68 129 86 1302.2 0 86 130 177 1302.2 0 177 131 78 1302.2 0 78 132 79 1302.2 0 79 133 62 1302.2 0 62 134 56 1302.2 0 56 135 79 1302.2 0 79 136 40 1302.2 0 40 137 77 1302.2 0 77 138 93 1302.2 0 93 139 86 1302.2 0 86 140 65 1302.2 0 65 141 87 1302.2 0 87 142 61 1302.2 0 61 143 50 1302.2 0 50 144 40 1302.2 0 40 145 54 1302.2 0 54 146 93 1302.2 0 93 147 79 1302.2 0 79 148 52 1302.2 0 52 149 29 1302.2 0 29 150 25 1302.2 0 25 151 31 1302.2 0 31 152 29 1302.2 0 29 153 37 1302.2 0 37 154 43 1302.2 0 43 155 72 1302.2 0 72 156 49 1302.2 0 49 157 62 1302.2 0 62 158 114 1302.2 0 114 159 94 1302.2 0 94 160 87 1302.2 0 87 161 104 1302.2 0 104 162 75 1302.2 0 75 163 85 1302.2 0 85 164 109 1302.2 0 109 165 130 1302.2 0 130 166 137 1302.2 0 137 167 166 1302.2 0 166 168 117 1302.2 0 117 169 78 1302.2 0 78 170 143 1302.2 0 143 171 76 1302.2 0 76 172 162 1302.2 0 162 173 138 1302.2 0 138 174 661 1302.2 0 661 175 568 1302.2 0 568 176 212 1302.2 0 212 177 108 1302.2 0 108 178 142 1302.2 0 142 179 162 1302.2 0 162 180 402 1302.2 0 402 181 364 1302.2 0 364 182 183 1302.2 0 183 183 136 1302.2 0 136 184 143 1302.2 0 143 185 126 1302.2 0 126 186 129 1302.2 0 129 187 104 1302.2 0 104 188 86 1302.2 0 86 189 48 1302.2 0 48 190 43 1302.2 0 43 191 60 1302.2 0 60 192 231 1302.2 0 231 193 216 1302.2 0 216 194 625 1302.2 0 625 195 276 1302.2 0 276 196 86 1302.2 0 86 197 59 1302.2 0 59 198 361 1302.2 0 361 199 3549 1302.2 0 3549 200 5941 1302.2 0 5941 201 193 1302.2 0 193 202 63 1302.2 0 63 203 40 1302.2 0 40 204 84 1302.2 0 84 205 120 1302.2 0 120 206 185 1302.2 0 185 207 99 1302.2 0 99 208 64 1302.2 0 64 209 52 1302.2 0 52 210 78 1302.2 0 78 211 85 1302.2 0 85 212 98 1302.2 0 98 213 83 1302.2 0 83 214 86 1302.2 0 86 215 84 1302.2 0 84 216 105 1302.2 0 105 217 90 1302.2 0 90 218 116 1302.2 0 116 219 374 1302.2 0 374 220 711 1302.2 0 711 221 279 1302.2 0 279 222 72 1302.2 0 72 223 44 1302.2 0 44 224 107 1302.2 0 107 225 157 1302.2 0 157 226 197 1302.2 0 197 227 173 1302.2 0 173 228 195 1302.2 0 195 229 98 1302.2 0 98 230 97 1302.2 0 97 231 55 1302.2 0 55 232 48 1302.2 0 48 233 35 1302.2 0 35 234 39 1302.2 0 39 235 60 1302.2 0 60 236 129 1302.2 0 129 237 95 1302.2 0 95 238 247 1302.2 0 247 239 246 1302.2 0 246 240 1300 1302.2 0 1300 241 232 1302.2 0 232 242 177 1302.2 0 177 243 48 1302.2 0 48 244 59 1302.2 0 59 245 131 1302.2 0 131 246 199 1302.2 0 199 247 217 1302.2 0 217 248 241 1302.2 0 241 249 138 1302.2 0 138 250 56 1302.2 0 56 251 43 1302.2 0 43 252 27 1302.2 0 27 253 48 1302.2 0 48 254 138 1302.2 0 138 255 183 1302.2 0 183 256 409 1302.2 0 409 257 546 1302.2 0 546 258 489 1302.2 0 489 259 63 1302.2 0 63 260 22 1302.2 0 22 261 22 1302.2 0 22 262 53 1302.2 0 53 263 455 1302.2 0 455 264 45 1302.2 0 45 265 61 1302.2 0 61 266 93 1302.2 0 93 267 185 1302.2 0 185 268 139 1302.2 0 139 269 110 1302.2 0 110 270 44 1302.2 0 44 271 34 1302.2 0 34 272 36 1302.2 0 36 273 42 1302.2 0 42 274 61 1302.2 0 61 275 61 1302.2 0 61 276 45 1302.2 0 45 277 32 1302.2 0 32 278 28 1302.2 0 28 279 56 1302.2 0 56 280 109 1302.2 0 109 281 89 1302.2 0 89 282 55 1302.2 0 55 283 41 1302.2 0 41 284 50 1302.2 0 50 285 137 1302.2 0 137 286 165 1302.2 0 165 287 108 1302.2 0 108 288 86 1302.2 0 86 289 40 1302.2 0 40 290 57 1302.2 0 57 291 60 1302.2 0 60 292 81 1302.2 0 81 293 104 1302.2 0 104 294 94 1302.2 0 94 295 58 1302.2 0 58 296 33 1302.2 0 33 297 45 1302.2 0 45 298 45 1302.2 0 45 299 25 1302.2 0 25 300 20 1302.2 0 20 301 19 1302.2 0 19 Finished in 3.11 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 70,074 Reads with adapters: 43,383 (61.9%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 43,361 (61.9%) Total basepairs processed: 16,570,502 bp Total written (filtered): 11,924,140 bp (72.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 43383 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 16 0.0 3 0 0 13 3 16 335 0.0 3 0 298 21 16 17 42110 0.0 3 40599 1340 130 41 18 176 0.0 3 3 86 80 7 19 146 0.0 3 3 9 19 115 20 553 0.0 3 490 19 0 44 21 2 0.0 3 0 1 1 22 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 95 8 0.0 3 0 0 0 8 96 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 105 5 0.0 3 0 0 0 5 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.99 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 43,361 Reads with adapters: 3,745 (8.6%) Reads written (passing filters): 43,361 (100.0%) Total basepairs processed: 11,924,140 bp Total written (filtered): 11,912,455 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3745 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.7% C: 6.4% G: 32.1% T: 16.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3297 677.5 0 3297 4 447 169.4 0 447 6 1 10.6 0 1