This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 174.48 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 96,539 (1.8%) Reads that were too short: 14,904 (0.3%) Reads written (passing filters): 81,635 (1.5%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 20,297,739 bp (1.3%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 96539 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1360 1302.2 0 1360 7 41958 1302.2 0 41958 8 126 1302.2 0 126 9 3979 1302.2 0 3979 10 12 1302.2 0 12 11 12 1302.2 0 12 12 14 1302.2 0 14 13 11 1302.2 0 11 14 7 1302.2 0 7 15 8 1302.2 0 8 16 26 1302.2 0 26 17 59 1302.2 0 59 18 54 1302.2 0 54 19 14 1302.2 0 14 20 21 1302.2 0 21 21 13 1302.2 0 13 22 11 1302.2 0 11 23 11 1302.2 0 11 24 13 1302.2 0 13 25 11 1302.2 0 11 26 13 1302.2 0 13 27 19 1302.2 0 19 28 13 1302.2 0 13 29 22 1302.2 0 22 30 15 1302.2 0 15 31 6 1302.2 0 6 32 13 1302.2 0 13 33 13 1302.2 0 13 34 10 1302.2 0 10 35 8 1302.2 0 8 36 9 1302.2 0 9 37 17 1302.2 0 17 38 15 1302.2 0 15 39 11 1302.2 0 11 40 83 1302.2 0 83 41 19 1302.2 0 19 42 19 1302.2 0 19 43 16 1302.2 0 16 44 14 1302.2 0 14 45 14 1302.2 0 14 46 23 1302.2 0 23 47 101 1302.2 0 101 48 437 1302.2 0 437 49 15 1302.2 0 15 50 15 1302.2 0 15 51 12 1302.2 0 12 52 14 1302.2 0 14 53 16 1302.2 0 16 54 14 1302.2 0 14 55 25 1302.2 0 25 56 281 1302.2 0 281 57 226 1302.2 0 226 58 314 1302.2 0 314 59 30 1302.2 0 30 60 11 1302.2 0 11 61 13 1302.2 0 13 62 9 1302.2 0 9 63 25 1302.2 0 25 64 12 1302.2 0 12 65 12 1302.2 0 12 66 18 1302.2 0 18 67 22 1302.2 0 22 68 20 1302.2 0 20 69 9 1302.2 0 9 70 18 1302.2 0 18 71 15 1302.2 0 15 72 12 1302.2 0 12 73 11 1302.2 0 11 74 21 1302.2 0 21 75 18 1302.2 0 18 76 21 1302.2 0 21 77 27 1302.2 0 27 78 79 1302.2 0 79 79 88 1302.2 0 88 80 237 1302.2 0 237 81 570 1302.2 0 570 82 375 1302.2 0 375 83 156 1302.2 0 156 84 216 1302.2 0 216 85 72 1302.2 0 72 86 61 1302.2 0 61 87 88 1302.2 0 88 88 53 1302.2 0 53 89 107 1302.2 0 107 90 99 1302.2 0 99 91 99 1302.2 0 99 92 112 1302.2 0 112 93 215 1302.2 0 215 94 3474 1302.2 0 3474 95 4500 1302.2 0 4500 96 6365 1302.2 0 6365 97 289 1302.2 0 289 98 178 1302.2 0 178 99 50 1302.2 0 50 100 148 1302.2 0 148 101 295 1302.2 0 295 102 58 1302.2 0 58 103 67 1302.2 0 67 104 20 1302.2 0 20 105 20 1302.2 0 20 106 32 1302.2 0 32 107 49 1302.2 0 49 108 48 1302.2 0 48 109 50 1302.2 0 50 110 367 1302.2 0 367 111 101 1302.2 0 101 112 247 1302.2 0 247 113 45 1302.2 0 45 114 35 1302.2 0 35 115 182 1302.2 0 182 116 78 1302.2 0 78 117 86 1302.2 0 86 118 148 1302.2 0 148 119 168 1302.2 0 168 120 124 1302.2 0 124 121 74 1302.2 0 74 122 73 1302.2 0 73 123 107 1302.2 0 107 124 98 1302.2 0 98 125 81 1302.2 0 81 126 98 1302.2 0 98 127 81 1302.2 0 81 128 77 1302.2 0 77 129 51 1302.2 0 51 130 90 1302.2 0 90 131 267 1302.2 0 267 132 364 1302.2 0 364 133 383 1302.2 0 383 134 128 1302.2 0 128 135 97 1302.2 0 97 136 185 1302.2 0 185 137 347 1302.2 0 347 138 356 1302.2 0 356 139 486 1302.2 0 486 140 302 1302.2 0 302 141 140 1302.2 0 140 142 76 1302.2 0 76 143 73 1302.2 0 73 144 97 1302.2 0 97 145 413 1302.2 0 413 146 340 1302.2 0 340 147 87 1302.2 0 87 148 56 1302.2 0 56 149 43 1302.2 0 43 150 55 1302.2 0 55 151 43 1302.2 0 43 152 36 1302.2 0 36 153 30 1302.2 0 30 154 66 1302.2 0 66 155 62 1302.2 0 62 156 99 1302.2 0 99 157 166 1302.2 0 166 158 174 1302.2 0 174 159 153 1302.2 0 153 160 159 1302.2 0 159 161 91 1302.2 0 91 162 63 1302.2 0 63 163 75 1302.2 0 75 164 75 1302.2 0 75 165 129 1302.2 0 129 166 157 1302.2 0 157 167 135 1302.2 0 135 168 112 1302.2 0 112 169 89 1302.2 0 89 170 154 1302.2 0 154 171 90 1302.2 0 90 172 105 1302.2 0 105 173 61 1302.2 0 61 174 135 1302.2 0 135 175 98 1302.2 0 98 176 88 1302.2 0 88 177 57 1302.2 0 57 178 35 1302.2 0 35 179 90 1302.2 0 90 180 173 1302.2 0 173 181 236 1302.2 0 236 182 154 1302.2 0 154 183 140 1302.2 0 140 184 126 1302.2 0 126 185 141 1302.2 0 141 186 168 1302.2 0 168 187 224 1302.2 0 224 188 110 1302.2 0 110 189 32 1302.2 0 32 190 55 1302.2 0 55 191 150 1302.2 0 150 192 499 1302.2 0 499 193 284 1302.2 0 284 194 871 1302.2 0 871 195 141 1302.2 0 141 196 58 1302.2 0 58 197 51 1302.2 0 51 198 118 1302.2 0 118 199 104 1302.2 0 104 200 80 1302.2 0 80 201 31 1302.2 0 31 202 32 1302.2 0 32 203 106 1302.2 0 106 204 268 1302.2 0 268 205 600 1302.2 0 600 206 485 1302.2 0 485 207 315 1302.2 0 315 208 87 1302.2 0 87 209 68 1302.2 0 68 210 89 1302.2 0 89 211 162 1302.2 0 162 212 225 1302.2 0 225 213 260 1302.2 0 260 214 190 1302.2 0 190 215 153 1302.2 0 153 216 117 1302.2 0 117 217 183 1302.2 0 183 218 219 1302.2 0 219 219 168 1302.2 0 168 220 175 1302.2 0 175 221 71 1302.2 0 71 222 44 1302.2 0 44 223 104 1302.2 0 104 224 191 1302.2 0 191 225 154 1302.2 0 154 226 179 1302.2 0 179 227 225 1302.2 0 225 228 263 1302.2 0 263 229 231 1302.2 0 231 230 115 1302.2 0 115 231 99 1302.2 0 99 232 86 1302.2 0 86 233 63 1302.2 0 63 234 122 1302.2 0 122 235 183 1302.2 0 183 236 222 1302.2 0 222 237 147 1302.2 0 147 238 195 1302.2 0 195 239 186 1302.2 0 186 240 124 1302.2 0 124 241 44 1302.2 0 44 242 37 1302.2 0 37 243 70 1302.2 0 70 244 134 1302.2 0 134 245 124 1302.2 0 124 246 78 1302.2 0 78 247 133 1302.2 0 133 248 56 1302.2 0 56 249 55 1302.2 0 55 250 20 1302.2 0 20 251 36 1302.2 0 36 252 98 1302.2 0 98 253 170 1302.2 0 170 254 190 1302.2 0 190 255 106 1302.2 0 106 256 148 1302.2 0 148 257 185 1302.2 0 185 258 113 1302.2 0 113 259 108 1302.2 0 108 260 39 1302.2 0 39 261 153 1302.2 0 153 262 203 1302.2 0 203 263 254 1302.2 0 254 264 149 1302.2 0 149 265 200 1302.2 0 200 266 185 1302.2 0 185 267 236 1302.2 0 236 268 171 1302.2 0 171 269 64 1302.2 0 64 270 24 1302.2 0 24 271 100 1302.2 0 100 272 400 1302.2 0 400 273 210 1302.2 0 210 274 157 1302.2 0 157 275 127 1302.2 0 127 276 89 1302.2 0 89 277 55 1302.2 0 55 278 83 1302.2 0 83 279 155 1302.2 0 155 280 189 1302.2 0 189 281 93 1302.2 0 93 282 70 1302.2 0 70 283 83 1302.2 0 83 284 140 1302.2 0 140 285 148 1302.2 0 148 286 177 1302.2 0 177 287 184 1302.2 0 184 288 289 1302.2 0 289 289 83 1302.2 0 83 290 90 1302.2 0 90 291 123 1302.2 0 123 292 171 1302.2 0 171 293 223 1302.2 0 223 294 128 1302.2 0 128 295 66 1302.2 0 66 296 51 1302.2 0 51 297 85 1302.2 0 85 298 160 1302.2 0 160 299 181 1302.2 0 181 300 153 1302.2 0 153 301 152 1302.2 0 152 Finished in 4.19 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 81,635 Reads with adapters: 42,710 (52.3%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 42,692 (52.3%) Total basepairs processed: 20,297,739 bp Total written (filtered): 11,697,503 bp (57.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42710 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 11 0.0 3 0 0 11 19 472 0.0 3 0 454 14 3 1 20 42034 0.0 4 41343 663 18 7 3 21 113 0.0 4 18 94 0 0 1 22 19 0.0 4 0 1 12 0 6 23 27 0.0 4 0 0 1 24 2 24 4 0.0 4 2 0 0 2 25 2 0.0 4 1 0 1 40 3 0.0 4 0 0 0 0 3 48 3 0.0 4 0 0 0 0 3 68 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 134 1 0.0 4 0 0 0 0 1 141 11 0.0 4 0 0 0 0 11 232 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.79 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 42,692 Reads with adapters: 365 (0.9%) Reads written (passing filters): 42,692 (100.0%) Total basepairs processed: 11,697,503 bp Total written (filtered): 11,692,548 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 365 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.3% C: 1.4% G: 93.2% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 667.1 0 9 4 3 166.8 0 3 5 2 41.7 0 2 6 1 10.4 0 1 11 333 0.0 1 0 333 12 12 0.0 1 0 12 152 2 0.0 1 1 1 256 1 0.0 1 1 260 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.