This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 170.78 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 178,405 (3.3%) Reads that were too short: 62,330 (1.2%) Reads written (passing filters): 116,075 (2.2%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 25,356,825 bp (1.6%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 178405 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 194 1302.2 0 194 7 413 1302.2 0 413 8 612 1302.2 0 612 9 33772 1302.2 0 33772 10 83 1302.2 0 83 11 36 1302.2 0 36 12 49 1302.2 0 49 13 47 1302.2 0 47 14 36 1302.2 0 36 15 42 1302.2 0 42 16 36 1302.2 0 36 17 50 1302.2 0 50 18 36 1302.2 0 36 19 45 1302.2 0 45 20 22 1302.2 0 22 21 54 1302.2 0 54 22 46 1302.2 0 46 23 31 1302.2 0 31 24 59 1302.2 0 59 25 52 1302.2 0 52 26 61 1302.2 0 61 27 40 1302.2 0 40 28 48 1302.2 0 48 29 40 1302.2 0 40 30 54 1302.2 0 54 31 79 1302.2 0 79 32 125 1302.2 0 125 33 96 1302.2 0 96 34 57 1302.2 0 57 35 366 1302.2 0 366 36 363 1302.2 0 363 37 496 1302.2 0 496 38 355 1302.2 0 355 39 67 1302.2 0 67 40 95 1302.2 0 95 41 116 1302.2 0 116 42 196 1302.2 0 196 43 6105 1302.2 0 6105 44 86 1302.2 0 86 45 31 1302.2 0 31 46 53 1302.2 0 53 47 49 1302.2 0 49 48 34 1302.2 0 34 49 34 1302.2 0 34 50 40 1302.2 0 40 51 37 1302.2 0 37 52 61 1302.2 0 61 53 35 1302.2 0 35 54 22 1302.2 0 22 55 40 1302.2 0 40 56 58 1302.2 0 58 57 32 1302.2 0 32 58 39 1302.2 0 39 59 53 1302.2 0 53 60 61 1302.2 0 61 61 35 1302.2 0 35 62 43 1302.2 0 43 63 40 1302.2 0 40 64 50 1302.2 0 50 65 45 1302.2 0 45 66 25 1302.2 0 25 67 44 1302.2 0 44 68 57 1302.2 0 57 69 260 1302.2 0 260 70 118 1302.2 0 118 71 225 1302.2 0 225 72 44 1302.2 0 44 73 41 1302.2 0 41 74 161 1302.2 0 161 75 94 1302.2 0 94 76 504 1302.2 0 504 77 209 1302.2 0 209 78 647 1302.2 0 647 79 191 1302.2 0 191 80 496 1302.2 0 496 81 446 1302.2 0 446 82 491 1302.2 0 491 83 119 1302.2 0 119 84 425 1302.2 0 425 85 174 1302.2 0 174 86 135 1302.2 0 135 87 708 1302.2 0 708 88 555 1302.2 0 555 89 597 1302.2 0 597 90 2552 1302.2 0 2552 91 202 1302.2 0 202 92 550 1302.2 0 550 93 447 1302.2 0 447 94 444 1302.2 0 444 95 471 1302.2 0 471 96 947 1302.2 0 947 97 2537 1302.2 0 2537 98 4647 1302.2 0 4647 99 7248 1302.2 0 7248 100 2514 1302.2 0 2514 101 1487 1302.2 0 1487 102 213 1302.2 0 213 103 144 1302.2 0 144 104 783 1302.2 0 783 105 315 1302.2 0 315 106 201 1302.2 0 201 107 373 1302.2 0 373 108 242 1302.2 0 242 109 474 1302.2 0 474 110 785 1302.2 0 785 111 1041 1302.2 0 1041 112 1952 1302.2 0 1952 113 424 1302.2 0 424 114 207 1302.2 0 207 115 359 1302.2 0 359 116 288 1302.2 0 288 117 155 1302.2 0 155 118 404 1302.2 0 404 119 226 1302.2 0 226 120 324 1302.2 0 324 121 446 1302.2 0 446 122 322 1302.2 0 322 123 290 1302.2 0 290 124 284 1302.2 0 284 125 321 1302.2 0 321 126 281 1302.2 0 281 127 372 1302.2 0 372 128 409 1302.2 0 409 129 640 1302.2 0 640 130 429 1302.2 0 429 131 546 1302.2 0 546 132 547 1302.2 0 547 133 673 1302.2 0 673 134 524 1302.2 0 524 135 464 1302.2 0 464 136 360 1302.2 0 360 137 320 1302.2 0 320 138 282 1302.2 0 282 139 648 1302.2 0 648 140 354 1302.2 0 354 141 479 1302.2 0 479 142 317 1302.2 0 317 143 370 1302.2 0 370 144 341 1302.2 0 341 145 292 1302.2 0 292 146 296 1302.2 0 296 147 407 1302.2 0 407 148 569 1302.2 0 569 149 422 1302.2 0 422 150 193 1302.2 0 193 151 213 1302.2 0 213 152 231 1302.2 0 231 153 225 1302.2 0 225 154 389 1302.2 0 389 155 577 1302.2 0 577 156 361 1302.2 0 361 157 417 1302.2 0 417 158 278 1302.2 0 278 159 547 1302.2 0 547 160 340 1302.2 0 340 161 336 1302.2 0 336 162 293 1302.2 0 293 163 296 1302.2 0 296 164 394 1302.2 0 394 165 350 1302.2 0 350 166 426 1302.2 0 426 167 432 1302.2 0 432 168 655 1302.2 0 655 169 257 1302.2 0 257 170 280 1302.2 0 280 171 360 1302.2 0 360 172 289 1302.2 0 289 173 323 1302.2 0 323 174 203 1302.2 0 203 175 242 1302.2 0 242 176 219 1302.2 0 219 177 338 1302.2 0 338 178 314 1302.2 0 314 179 271 1302.2 0 271 180 213 1302.2 0 213 181 218 1302.2 0 218 182 356 1302.2 0 356 183 406 1302.2 0 406 184 389 1302.2 0 389 185 367 1302.2 0 367 186 205 1302.2 0 205 187 259 1302.2 0 259 188 246 1302.2 0 246 189 427 1302.2 0 427 190 440 1302.2 0 440 191 404 1302.2 0 404 192 427 1302.2 0 427 193 480 1302.2 0 480 194 342 1302.2 0 342 195 745 1302.2 0 745 196 482 1302.2 0 482 197 490 1302.2 0 490 198 222 1302.2 0 222 199 202 1302.2 0 202 200 530 1302.2 0 530 201 997 1302.2 0 997 202 1151 1302.2 0 1151 203 1017 1302.2 0 1017 204 444 1302.2 0 444 205 447 1302.2 0 447 206 801 1302.2 0 801 207 1469 1302.2 0 1469 208 1009 1302.2 0 1009 209 974 1302.2 0 974 210 403 1302.2 0 403 211 509 1302.2 0 509 212 579 1302.2 0 579 213 442 1302.2 0 442 214 581 1302.2 0 581 215 484 1302.2 0 484 216 468 1302.2 0 468 217 409 1302.2 0 409 218 247 1302.2 0 247 219 202 1302.2 0 202 220 573 1302.2 0 573 221 737 1302.2 0 737 222 577 1302.2 0 577 223 628 1302.2 0 628 224 417 1302.2 0 417 225 394 1302.2 0 394 226 326 1302.2 0 326 227 429 1302.2 0 429 228 331 1302.2 0 331 229 486 1302.2 0 486 230 436 1302.2 0 436 231 673 1302.2 0 673 232 525 1302.2 0 525 233 619 1302.2 0 619 234 590 1302.2 0 590 235 903 1302.2 0 903 236 447 1302.2 0 447 237 997 1302.2 0 997 238 543 1302.2 0 543 239 772 1302.2 0 772 240 808 1302.2 0 808 241 1781 1302.2 0 1781 242 1116 1302.2 0 1116 243 671 1302.2 0 671 244 324 1302.2 0 324 245 217 1302.2 0 217 246 260 1302.2 0 260 247 237 1302.2 0 237 248 371 1302.2 0 371 249 771 1302.2 0 771 250 1130 1302.2 0 1130 251 512 1302.2 0 512 252 246 1302.2 0 246 253 227 1302.2 0 227 254 208 1302.2 0 208 255 188 1302.2 0 188 256 168 1302.2 0 168 257 324 1302.2 0 324 258 434 1302.2 0 434 259 780 1302.2 0 780 260 1482 1302.2 0 1482 261 2355 1302.2 0 2355 262 1731 1302.2 0 1731 263 659 1302.2 0 659 264 387 1302.2 0 387 265 531 1302.2 0 531 266 241 1302.2 0 241 267 239 1302.2 0 239 268 324 1302.2 0 324 269 414 1302.2 0 414 270 668 1302.2 0 668 271 463 1302.2 0 463 272 513 1302.2 0 513 273 498 1302.2 0 498 274 788 1302.2 0 788 275 896 1302.2 0 896 276 430 1302.2 0 430 277 339 1302.2 0 339 278 274 1302.2 0 274 279 262 1302.2 0 262 280 342 1302.2 0 342 281 404 1302.2 0 404 282 476 1302.2 0 476 283 495 1302.2 0 495 284 285 1302.2 0 285 285 376 1302.2 0 376 286 427 1302.2 0 427 287 369 1302.2 0 369 288 603 1302.2 0 603 289 758 1302.2 0 758 290 615 1302.2 0 615 291 558 1302.2 0 558 292 491 1302.2 0 491 293 394 1302.2 0 394 294 408 1302.2 0 408 295 582 1302.2 0 582 296 521 1302.2 0 521 297 1173 1302.2 0 1173 298 1929 1302.2 0 1929 299 1004 1302.2 0 1004 300 1204 1302.2 0 1204 301 1610 1302.2 0 1610 Finished in 5.17 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 116,075 Reads with adapters: 34,763 (29.9%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 34,744 (29.9%) Total basepairs processed: 25,356,825 bp Total written (filtered): 9,545,077 bp (37.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 34763 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 15 0.0 3 0 0 15 16 350 0.0 3 0 325 18 7 17 34157 0.0 3 32881 1128 115 33 18 99 0.0 3 11 64 17 7 19 35 0.0 3 1 0 11 23 20 12 0.0 3 0 0 0 12 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 1 34 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 1 53 1 0.0 3 1 54 1 0.0 3 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 81 8 0.0 3 0 0 0 8 84 6 0.0 3 0 0 0 6 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 95 5 0.0 3 0 0 0 5 96 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 1 2 100 1 0.0 3 0 0 0 1 103 4 0.0 3 0 0 0 4 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 3 0.0 3 0 0 1 2 145 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 148 3 0.0 3 0 0 0 3 150 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 3 0.0 3 0 0 0 3 214 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.54 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 34,744 Reads with adapters: 1,233 (3.5%) Reads written (passing filters): 34,744 (100.0%) Total basepairs processed: 9,545,077 bp Total written (filtered): 9,541,177 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1233 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.1% C: 17.3% G: 40.6% T: 13.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1033 542.9 0 1033 4 199 135.7 0 199 5 1 33.9 0 1