This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 169.33 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 113,114 (2.1%) Reads that were too short: 27,362 (0.5%) Reads written (passing filters): 85,752 (1.6%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 16,581,767 bp (1.0%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 113114 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 50 1302.2 0 50 7 97 1302.2 0 97 8 3821 1302.2 0 3821 9 55 1302.2 0 55 10 77 1302.2 0 77 11 21 1302.2 0 21 12 36 1302.2 0 36 13 16 1302.2 0 16 14 14 1302.2 0 14 15 13 1302.2 0 13 16 9 1302.2 0 9 17 6 1302.2 0 6 18 14 1302.2 0 14 19 19 1302.2 0 19 20 11 1302.2 0 11 21 12 1302.2 0 12 22 12 1302.2 0 12 23 9 1302.2 0 9 24 12 1302.2 0 12 25 17 1302.2 0 17 26 21 1302.2 0 21 27 13 1302.2 0 13 28 19 1302.2 0 19 29 17 1302.2 0 17 30 36 1302.2 0 36 31 16 1302.2 0 16 32 20 1302.2 0 20 33 16 1302.2 0 16 34 13 1302.2 0 13 35 69 1302.2 0 69 36 34 1302.2 0 34 37 18 1302.2 0 18 38 34 1302.2 0 34 39 12 1302.2 0 12 40 8 1302.2 0 8 41 19 1302.2 0 19 42 25 1302.2 0 25 43 9 1302.2 0 9 44 14 1302.2 0 14 45 11 1302.2 0 11 46 25 1302.2 0 25 47 10 1302.2 0 10 48 24 1302.2 0 24 49 24 1302.2 0 24 50 12 1302.2 0 12 51 22 1302.2 0 22 52 12 1302.2 0 12 53 17 1302.2 0 17 54 74 1302.2 0 74 55 114 1302.2 0 114 56 12 1302.2 0 12 57 10 1302.2 0 10 58 14 1302.2 0 14 59 12 1302.2 0 12 60 14 1302.2 0 14 61 26 1302.2 0 26 62 13 1302.2 0 13 63 36 1302.2 0 36 64 112 1302.2 0 112 65 38 1302.2 0 38 66 43 1302.2 0 43 67 27 1302.2 0 27 68 10 1302.2 0 10 69 32 1302.2 0 32 70 16 1302.2 0 16 71 48 1302.2 0 48 72 29 1302.2 0 29 73 66 1302.2 0 66 74 54 1302.2 0 54 75 130 1302.2 0 130 76 225 1302.2 0 225 77 258 1302.2 0 258 78 350 1302.2 0 350 79 624 1302.2 0 624 80 1069 1302.2 0 1069 81 16578 1302.2 0 16578 82 5524 1302.2 0 5524 83 9288 1302.2 0 9288 84 755 1302.2 0 755 85 474 1302.2 0 474 86 419 1302.2 0 419 87 512 1302.2 0 512 88 446 1302.2 0 446 89 412 1302.2 0 412 90 48 1302.2 0 48 91 136 1302.2 0 136 92 439 1302.2 0 439 93 146 1302.2 0 146 94 249 1302.2 0 249 95 131 1302.2 0 131 96 68 1302.2 0 68 97 92 1302.2 0 92 98 171 1302.2 0 171 99 270 1302.2 0 270 100 78 1302.2 0 78 101 57 1302.2 0 57 102 88 1302.2 0 88 103 77 1302.2 0 77 104 81 1302.2 0 81 105 34 1302.2 0 34 106 35 1302.2 0 35 107 19 1302.2 0 19 108 56 1302.2 0 56 109 196 1302.2 0 196 110 78 1302.2 0 78 111 80 1302.2 0 80 112 136 1302.2 0 136 113 72 1302.2 0 72 114 88 1302.2 0 88 115 134 1302.2 0 134 116 127 1302.2 0 127 117 277 1302.2 0 277 118 428 1302.2 0 428 119 303 1302.2 0 303 120 741 1302.2 0 741 121 547 1302.2 0 547 122 2134 1302.2 0 2134 123 6087 1302.2 0 6087 124 8338 1302.2 0 8338 125 280 1302.2 0 280 126 314 1302.2 0 314 127 141 1302.2 0 141 128 141 1302.2 0 141 129 182 1302.2 0 182 130 287 1302.2 0 287 131 217 1302.2 0 217 132 164 1302.2 0 164 133 270 1302.2 0 270 134 212 1302.2 0 212 135 283 1302.2 0 283 136 255 1302.2 0 255 137 242 1302.2 0 242 138 227 1302.2 0 227 139 250 1302.2 0 250 140 157 1302.2 0 157 141 154 1302.2 0 154 142 162 1302.2 0 162 143 312 1302.2 0 312 144 631 1302.2 0 631 145 228 1302.2 0 228 146 711 1302.2 0 711 147 198 1302.2 0 198 148 146 1302.2 0 146 149 112 1302.2 0 112 150 96 1302.2 0 96 151 343 1302.2 0 343 152 258 1302.2 0 258 153 305 1302.2 0 305 154 259 1302.2 0 259 155 303 1302.2 0 303 156 370 1302.2 0 370 157 331 1302.2 0 331 158 247 1302.2 0 247 159 312 1302.2 0 312 160 236 1302.2 0 236 161 271 1302.2 0 271 162 257 1302.2 0 257 163 286 1302.2 0 286 164 275 1302.2 0 275 165 265 1302.2 0 265 166 235 1302.2 0 235 167 253 1302.2 0 253 168 319 1302.2 0 319 169 159 1302.2 0 159 170 204 1302.2 0 204 171 262 1302.2 0 262 172 379 1302.2 0 379 173 271 1302.2 0 271 174 103 1302.2 0 103 175 93 1302.2 0 93 176 164 1302.2 0 164 177 377 1302.2 0 377 178 420 1302.2 0 420 179 345 1302.2 0 345 180 200 1302.2 0 200 181 221 1302.2 0 221 182 837 1302.2 0 837 183 323 1302.2 0 323 184 757 1302.2 0 757 185 206 1302.2 0 206 186 131 1302.2 0 131 187 123 1302.2 0 123 188 192 1302.2 0 192 189 423 1302.2 0 423 190 661 1302.2 0 661 191 245 1302.2 0 245 192 136 1302.2 0 136 193 126 1302.2 0 126 194 157 1302.2 0 157 195 226 1302.2 0 226 196 417 1302.2 0 417 197 616 1302.2 0 616 198 202 1302.2 0 202 199 79 1302.2 0 79 200 139 1302.2 0 139 201 265 1302.2 0 265 202 363 1302.2 0 363 203 245 1302.2 0 245 204 173 1302.2 0 173 205 252 1302.2 0 252 206 178 1302.2 0 178 207 166 1302.2 0 166 208 273 1302.2 0 273 209 267 1302.2 0 267 210 252 1302.2 0 252 211 281 1302.2 0 281 212 222 1302.2 0 222 213 239 1302.2 0 239 214 260 1302.2 0 260 215 279 1302.2 0 279 216 336 1302.2 0 336 217 347 1302.2 0 347 218 171 1302.2 0 171 219 140 1302.2 0 140 220 174 1302.2 0 174 221 312 1302.2 0 312 222 608 1302.2 0 608 223 370 1302.2 0 370 224 249 1302.2 0 249 225 224 1302.2 0 224 226 235 1302.2 0 235 227 176 1302.2 0 176 228 168 1302.2 0 168 229 122 1302.2 0 122 230 134 1302.2 0 134 231 117 1302.2 0 117 232 157 1302.2 0 157 233 287 1302.2 0 287 234 274 1302.2 0 274 235 112 1302.2 0 112 236 121 1302.2 0 121 237 140 1302.2 0 140 238 168 1302.2 0 168 239 171 1302.2 0 171 240 667 1302.2 0 667 241 381 1302.2 0 381 242 709 1302.2 0 709 243 407 1302.2 0 407 244 258 1302.2 0 258 245 263 1302.2 0 263 246 182 1302.2 0 182 247 75 1302.2 0 75 248 78 1302.2 0 78 249 171 1302.2 0 171 250 312 1302.2 0 312 251 514 1302.2 0 514 252 559 1302.2 0 559 253 340 1302.2 0 340 254 284 1302.2 0 284 255 188 1302.2 0 188 256 128 1302.2 0 128 257 97 1302.2 0 97 258 75 1302.2 0 75 259 114 1302.2 0 114 260 129 1302.2 0 129 261 175 1302.2 0 175 262 557 1302.2 0 557 263 555 1302.2 0 555 264 688 1302.2 0 688 265 430 1302.2 0 430 266 244 1302.2 0 244 267 161 1302.2 0 161 268 162 1302.2 0 162 269 194 1302.2 0 194 270 286 1302.2 0 286 271 264 1302.2 0 264 272 241 1302.2 0 241 273 147 1302.2 0 147 274 142 1302.2 0 142 275 193 1302.2 0 193 276 325 1302.2 0 325 277 427 1302.2 0 427 278 338 1302.2 0 338 279 186 1302.2 0 186 280 154 1302.2 0 154 281 149 1302.2 0 149 282 182 1302.2 0 182 283 312 1302.2 0 312 284 199 1302.2 0 199 285 269 1302.2 0 269 286 122 1302.2 0 122 287 132 1302.2 0 132 288 178 1302.2 0 178 289 243 1302.2 0 243 290 496 1302.2 0 496 291 1039 1302.2 0 1039 292 557 1302.2 0 557 293 297 1302.2 0 297 294 272 1302.2 0 272 295 286 1302.2 0 286 296 580 1302.2 0 580 297 613 1302.2 0 613 298 356 1302.2 0 356 299 222 1302.2 0 222 300 168 1302.2 0 168 301 127 1302.2 0 127 Finished in 3.65 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 85,752 Reads with adapters: 4,107 (4.8%) Reads that were too short: 152 (0.2%) Reads written (passing filters): 3,955 (4.6%) Total basepairs processed: 16,581,767 bp Total written (filtered): 1,076,205 bp (6.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4107 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 1 4 16 34 0.0 3 0 21 2 11 17 3700 0.0 3 3583 99 14 4 18 59 0.0 3 2 8 43 6 19 43 0.0 3 0 0 18 25 20 13 0.0 3 1 0 0 12 22 2 0.0 3 0 0 0 2 25 2 0.0 3 0 0 0 2 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 1 34 5 0.0 3 0 0 0 5 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 9 0.0 3 0 0 0 9 50 32 0.0 3 0 0 0 32 51 16 0.0 3 0 0 0 16 52 5 0.0 3 0 0 0 5 53 6 0.0 3 0 0 0 6 64 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 3 0.0 3 0 0 0 3 72 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 161 11 0.0 3 0 0 0 11 162 36 0.0 3 0 0 0 36 163 9 0.0 3 0 0 0 9 164 15 0.0 3 0 0 1 14 165 58 0.0 3 0 0 2 56 166 7 0.0 3 0 0 0 7 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 209 4 0.0 3 0 0 0 4 215 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 3,955 Reads with adapters: 142 (3.6%) Reads written (passing filters): 3,955 (100.0%) Total basepairs processed: 1,076,205 bp Total written (filtered): 1,075,746 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 142 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.4% C: 20.4% G: 16.9% T: 18.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 112 61.8 0 112 4 28 15.4 0 28 5 1 3.9 0 1 6 1 1.0 0 1