This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 168.19 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 51,198 (1.0%) Reads that were too short: 21,818 (0.4%) Reads written (passing filters): 29,380 (0.6%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 5,457,842 bp (0.3%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 51198 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 32 1302.2 0 32 7 65 1302.2 0 65 8 992 1302.2 0 992 9 3640 1302.2 0 3640 10 15 1302.2 0 15 11 6 1302.2 0 6 12 10 1302.2 0 10 13 12 1302.2 0 12 14 3 1302.2 0 3 15 2 1302.2 0 2 16 9 1302.2 0 9 17 6 1302.2 0 6 18 10 1302.2 0 10 19 13 1302.2 0 13 20 6 1302.2 0 6 21 10 1302.2 0 10 22 5 1302.2 0 5 23 10 1302.2 0 10 24 11 1302.2 0 11 25 7 1302.2 0 7 26 10 1302.2 0 10 27 10 1302.2 0 10 28 11 1302.2 0 11 29 8 1302.2 0 8 30 10 1302.2 0 10 31 11 1302.2 0 11 32 13 1302.2 0 13 33 12 1302.2 0 12 34 15 1302.2 0 15 35 6 1302.2 0 6 36 9 1302.2 0 9 37 15 1302.2 0 15 38 39 1302.2 0 39 39 16 1302.2 0 16 40 20 1302.2 0 20 41 7 1302.2 0 7 42 11 1302.2 0 11 43 18 1302.2 0 18 44 18 1302.2 0 18 45 9 1302.2 0 9 46 63 1302.2 0 63 47 31 1302.2 0 31 48 10 1302.2 0 10 49 14 1302.2 0 14 50 15 1302.2 0 15 51 20 1302.2 0 20 52 13 1302.2 0 13 53 14 1302.2 0 14 54 16 1302.2 0 16 55 19 1302.2 0 19 56 12 1302.2 0 12 57 13 1302.2 0 13 58 7 1302.2 0 7 59 6 1302.2 0 6 60 10 1302.2 0 10 61 7 1302.2 0 7 62 6 1302.2 0 6 63 32 1302.2 0 32 64 8 1302.2 0 8 65 30 1302.2 0 30 66 17 1302.2 0 17 67 18 1302.2 0 18 68 16 1302.2 0 16 69 7 1302.2 0 7 70 12 1302.2 0 12 71 17 1302.2 0 17 72 23 1302.2 0 23 73 16 1302.2 0 16 74 43 1302.2 0 43 75 26 1302.2 0 26 76 25 1302.2 0 25 77 22 1302.2 0 22 78 57 1302.2 0 57 79 34 1302.2 0 34 80 38 1302.2 0 38 81 21 1302.2 0 21 82 20 1302.2 0 20 83 36 1302.2 0 36 84 19 1302.2 0 19 85 30 1302.2 0 30 86 24 1302.2 0 24 87 15 1302.2 0 15 88 26 1302.2 0 26 89 13 1302.2 0 13 90 12 1302.2 0 12 91 24 1302.2 0 24 92 24 1302.2 0 24 93 43 1302.2 0 43 94 100 1302.2 0 100 95 480 1302.2 0 480 96 179 1302.2 0 179 97 371 1302.2 0 371 98 285 1302.2 0 285 99 452 1302.2 0 452 100 266 1302.2 0 266 101 112 1302.2 0 112 102 176 1302.2 0 176 103 31 1302.2 0 31 104 37 1302.2 0 37 105 38 1302.2 0 38 106 55 1302.2 0 55 107 58 1302.2 0 58 108 60 1302.2 0 60 109 141 1302.2 0 141 110 112 1302.2 0 112 111 400 1302.2 0 400 112 7703 1302.2 0 7703 113 304 1302.2 0 304 114 18 1302.2 0 18 115 38 1302.2 0 38 116 41 1302.2 0 41 117 88 1302.2 0 88 118 35 1302.2 0 35 119 23 1302.2 0 23 120 119 1302.2 0 119 121 65 1302.2 0 65 122 67 1302.2 0 67 123 39 1302.2 0 39 124 26 1302.2 0 26 125 49 1302.2 0 49 126 69 1302.2 0 69 127 70 1302.2 0 70 128 37 1302.2 0 37 129 51 1302.2 0 51 130 71 1302.2 0 71 131 59 1302.2 0 59 132 162 1302.2 0 162 133 200 1302.2 0 200 134 324 1302.2 0 324 135 170 1302.2 0 170 136 206 1302.2 0 206 137 104 1302.2 0 104 138 83 1302.2 0 83 139 87 1302.2 0 87 140 214 1302.2 0 214 141 54 1302.2 0 54 142 62 1302.2 0 62 143 18 1302.2 0 18 144 45 1302.2 0 45 145 50 1302.2 0 50 146 151 1302.2 0 151 147 103 1302.2 0 103 148 72 1302.2 0 72 149 55 1302.2 0 55 150 36 1302.2 0 36 151 38 1302.2 0 38 152 20 1302.2 0 20 153 21 1302.2 0 21 154 28 1302.2 0 28 155 60 1302.2 0 60 156 182 1302.2 0 182 157 93 1302.2 0 93 158 55 1302.2 0 55 159 74 1302.2 0 74 160 74 1302.2 0 74 161 62 1302.2 0 62 162 67 1302.2 0 67 163 45 1302.2 0 45 164 72 1302.2 0 72 165 65 1302.2 0 65 166 265 1302.2 0 265 167 138 1302.2 0 138 168 192 1302.2 0 192 169 126 1302.2 0 126 170 64 1302.2 0 64 171 46 1302.2 0 46 172 54 1302.2 0 54 173 172 1302.2 0 172 174 160 1302.2 0 160 175 206 1302.2 0 206 176 149 1302.2 0 149 177 44 1302.2 0 44 178 50 1302.2 0 50 179 228 1302.2 0 228 180 183 1302.2 0 183 181 440 1302.2 0 440 182 236 1302.2 0 236 183 138 1302.2 0 138 184 117 1302.2 0 117 185 248 1302.2 0 248 186 322 1302.2 0 322 187 145 1302.2 0 145 188 31 1302.2 0 31 189 28 1302.2 0 28 190 39 1302.2 0 39 191 66 1302.2 0 66 192 138 1302.2 0 138 193 695 1302.2 0 695 194 651 1302.2 0 651 195 524 1302.2 0 524 196 473 1302.2 0 473 197 247 1302.2 0 247 198 122 1302.2 0 122 199 320 1302.2 0 320 200 298 1302.2 0 298 201 392 1302.2 0 392 202 286 1302.2 0 286 203 100 1302.2 0 100 204 124 1302.2 0 124 205 159 1302.2 0 159 206 169 1302.2 0 169 207 188 1302.2 0 188 208 148 1302.2 0 148 209 108 1302.2 0 108 210 104 1302.2 0 104 211 136 1302.2 0 136 212 221 1302.2 0 221 213 139 1302.2 0 139 214 112 1302.2 0 112 215 225 1302.2 0 225 216 270 1302.2 0 270 217 376 1302.2 0 376 218 178 1302.2 0 178 219 227 1302.2 0 227 220 507 1302.2 0 507 221 692 1302.2 0 692 222 1263 1302.2 0 1263 223 215 1302.2 0 215 224 54 1302.2 0 54 225 125 1302.2 0 125 226 153 1302.2 0 153 227 215 1302.2 0 215 228 260 1302.2 0 260 229 181 1302.2 0 181 230 115 1302.2 0 115 231 55 1302.2 0 55 232 39 1302.2 0 39 233 64 1302.2 0 64 234 78 1302.2 0 78 235 70 1302.2 0 70 236 115 1302.2 0 115 237 228 1302.2 0 228 238 124 1302.2 0 124 239 225 1302.2 0 225 240 54 1302.2 0 54 241 64 1302.2 0 64 242 99 1302.2 0 99 243 150 1302.2 0 150 244 145 1302.2 0 145 245 225 1302.2 0 225 246 171 1302.2 0 171 247 132 1302.2 0 132 248 73 1302.2 0 73 249 53 1302.2 0 53 250 21 1302.2 0 21 251 39 1302.2 0 39 252 81 1302.2 0 81 253 100 1302.2 0 100 254 361 1302.2 0 361 255 605 1302.2 0 605 256 1234 1302.2 0 1234 257 347 1302.2 0 347 258 262 1302.2 0 262 259 155 1302.2 0 155 260 102 1302.2 0 102 261 66 1302.2 0 66 262 431 1302.2 0 431 263 2477 1302.2 0 2477 264 2506 1302.2 0 2506 265 177 1302.2 0 177 266 276 1302.2 0 276 267 222 1302.2 0 222 268 306 1302.2 0 306 269 112 1302.2 0 112 270 207 1302.2 0 207 271 46 1302.2 0 46 272 55 1302.2 0 55 273 95 1302.2 0 95 274 83 1302.2 0 83 275 114 1302.2 0 114 276 60 1302.2 0 60 277 50 1302.2 0 50 278 53 1302.2 0 53 279 116 1302.2 0 116 280 187 1302.2 0 187 281 147 1302.2 0 147 282 70 1302.2 0 70 283 73 1302.2 0 73 284 91 1302.2 0 91 285 105 1302.2 0 105 286 205 1302.2 0 205 287 191 1302.2 0 191 288 35 1302.2 0 35 289 20 1302.2 0 20 290 34 1302.2 0 34 291 37 1302.2 0 37 292 63 1302.2 0 63 293 70 1302.2 0 70 294 113 1302.2 0 113 295 158 1302.2 0 158 296 32 1302.2 0 32 297 16 1302.2 0 16 298 18 1302.2 0 18 299 37 1302.2 0 37 300 46 1302.2 0 46 301 97 1302.2 0 97 Finished in 1.41 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 29,380 Reads with adapters: 3,762 (12.8%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 3,756 (12.8%) Total basepairs processed: 5,457,842 bp Total written (filtered): 1,020,372 bp (18.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3762 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 2 0.0 3 0 0 2 19 82 0.0 3 0 76 3 0 3 20 3603 0.0 4 3555 44 2 1 1 21 15 0.0 4 1 10 0 2 2 22 1 0.0 4 0 0 0 0 1 23 19 0.0 4 0 0 0 17 2 24 20 0.0 4 0 1 0 1 18 31 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 127 11 0.0 4 0 0 0 0 11 130 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 3,756 Reads with adapters: 517 (13.8%) Reads written (passing filters): 3,756 (100.0%) Total basepairs processed: 1,020,372 bp Total written (filtered): 899,319 bp (88.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 517 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 81.8% C: 8.3% G: 9.7% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 58.7 0 1 4 5 14.7 0 5 5 4 3.7 0 4 6 6 0.9 0 6 9 2 0.0 0 0 2 10 19 0.0 1 0 19 11 14 0.0 1 0 14 256 41 0.0 1 40 1 257 200 0.0 1 184 16 258 10 0.0 1 10 261 205 0.0 1 193 12 262 7 0.0 1 6 1 263 2 0.0 1 2 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.