This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 161.59 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 155,248 (2.9%) Reads that were too short: 15,176 (0.3%) Reads written (passing filters): 140,072 (2.6%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 25,504,761 bp (1.6%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 155248 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 219 1302.2 0 219 7 923 1302.2 0 923 8 208 1302.2 0 208 9 6492 1302.2 0 6492 10 35 1302.2 0 35 11 28 1302.2 0 28 12 16 1302.2 0 16 13 11 1302.2 0 11 14 86 1302.2 0 86 15 40 1302.2 0 40 16 13 1302.2 0 13 17 6 1302.2 0 6 18 10 1302.2 0 10 19 15 1302.2 0 15 20 14 1302.2 0 14 21 15 1302.2 0 15 22 9 1302.2 0 9 23 16 1302.2 0 16 24 32 1302.2 0 32 25 11 1302.2 0 11 26 11 1302.2 0 11 27 14 1302.2 0 14 28 9 1302.2 0 9 29 18 1302.2 0 18 30 13 1302.2 0 13 31 12 1302.2 0 12 32 11 1302.2 0 11 33 26 1302.2 0 26 34 25 1302.2 0 25 35 13 1302.2 0 13 36 19 1302.2 0 19 37 71 1302.2 0 71 38 12 1302.2 0 12 39 26 1302.2 0 26 40 51 1302.2 0 51 41 108 1302.2 0 108 42 14 1302.2 0 14 43 58 1302.2 0 58 44 17 1302.2 0 17 45 39 1302.2 0 39 46 16 1302.2 0 16 47 12 1302.2 0 12 48 19 1302.2 0 19 49 15 1302.2 0 15 50 17 1302.2 0 17 51 15 1302.2 0 15 52 25 1302.2 0 25 53 13 1302.2 0 13 54 25 1302.2 0 25 55 13 1302.2 0 13 56 37 1302.2 0 37 57 28 1302.2 0 28 58 84 1302.2 0 84 59 53 1302.2 0 53 60 13 1302.2 0 13 61 34 1302.2 0 34 62 11 1302.2 0 11 63 21 1302.2 0 21 64 10 1302.2 0 10 65 16 1302.2 0 16 66 6 1302.2 0 6 67 9 1302.2 0 9 68 14 1302.2 0 14 69 10 1302.2 0 10 70 23 1302.2 0 23 71 12 1302.2 0 12 72 10 1302.2 0 10 73 8 1302.2 0 8 74 19 1302.2 0 19 75 20 1302.2 0 20 76 20 1302.2 0 20 77 132 1302.2 0 132 78 34 1302.2 0 34 79 46 1302.2 0 46 80 49 1302.2 0 49 81 124 1302.2 0 124 82 61 1302.2 0 61 83 519 1302.2 0 519 84 275 1302.2 0 275 85 246 1302.2 0 246 86 52 1302.2 0 52 87 165 1302.2 0 165 88 52 1302.2 0 52 89 91 1302.2 0 91 90 121 1302.2 0 121 91 187 1302.2 0 187 92 223 1302.2 0 223 93 198 1302.2 0 198 94 403 1302.2 0 403 95 58 1302.2 0 58 96 128 1302.2 0 128 97 437 1302.2 0 437 98 90 1302.2 0 90 99 149 1302.2 0 149 100 82 1302.2 0 82 101 50 1302.2 0 50 102 178 1302.2 0 178 103 97 1302.2 0 97 104 118 1302.2 0 118 105 97 1302.2 0 97 106 130 1302.2 0 130 107 318 1302.2 0 318 108 522 1302.2 0 522 109 426 1302.2 0 426 110 694 1302.2 0 694 111 733 1302.2 0 733 112 1178 1302.2 0 1178 113 2139 1302.2 0 2139 114 33909 1302.2 0 33909 115 5752 1302.2 0 5752 116 37044 1302.2 0 37044 117 310 1302.2 0 310 118 115 1302.2 0 115 119 59 1302.2 0 59 120 55 1302.2 0 55 121 46 1302.2 0 46 122 126 1302.2 0 126 123 82 1302.2 0 82 124 94 1302.2 0 94 125 72 1302.2 0 72 126 132 1302.2 0 132 127 64 1302.2 0 64 128 458 1302.2 0 458 129 333 1302.2 0 333 130 183 1302.2 0 183 131 217 1302.2 0 217 132 79 1302.2 0 79 133 114 1302.2 0 114 134 83 1302.2 0 83 135 182 1302.2 0 182 136 316 1302.2 0 316 137 261 1302.2 0 261 138 272 1302.2 0 272 139 146 1302.2 0 146 140 455 1302.2 0 455 141 1060 1302.2 0 1060 142 1290 1302.2 0 1290 143 339 1302.2 0 339 144 216 1302.2 0 216 145 363 1302.2 0 363 146 429 1302.2 0 429 147 7147 1302.2 0 7147 148 992 1302.2 0 992 149 5007 1302.2 0 5007 150 305 1302.2 0 305 151 281 1302.2 0 281 152 468 1302.2 0 468 153 6430 1302.2 0 6430 154 1359 1302.2 0 1359 155 4074 1302.2 0 4074 156 906 1302.2 0 906 157 792 1302.2 0 792 158 313 1302.2 0 313 159 93 1302.2 0 93 160 173 1302.2 0 173 161 194 1302.2 0 194 162 282 1302.2 0 282 163 216 1302.2 0 216 164 238 1302.2 0 238 165 276 1302.2 0 276 166 194 1302.2 0 194 167 258 1302.2 0 258 168 812 1302.2 0 812 169 522 1302.2 0 522 170 362 1302.2 0 362 171 154 1302.2 0 154 172 92 1302.2 0 92 173 55 1302.2 0 55 174 69 1302.2 0 69 175 131 1302.2 0 131 176 179 1302.2 0 179 177 60 1302.2 0 60 178 88 1302.2 0 88 179 92 1302.2 0 92 180 108 1302.2 0 108 181 300 1302.2 0 300 182 255 1302.2 0 255 183 315 1302.2 0 315 184 245 1302.2 0 245 185 275 1302.2 0 275 186 172 1302.2 0 172 187 192 1302.2 0 192 188 52 1302.2 0 52 189 45 1302.2 0 45 190 57 1302.2 0 57 191 96 1302.2 0 96 192 132 1302.2 0 132 193 104 1302.2 0 104 194 83 1302.2 0 83 195 38 1302.2 0 38 196 56 1302.2 0 56 197 80 1302.2 0 80 198 52 1302.2 0 52 199 45 1302.2 0 45 200 44 1302.2 0 44 201 60 1302.2 0 60 202 447 1302.2 0 447 203 188 1302.2 0 188 204 468 1302.2 0 468 205 217 1302.2 0 217 206 110 1302.2 0 110 207 73 1302.2 0 73 208 46 1302.2 0 46 209 58 1302.2 0 58 210 123 1302.2 0 123 211 86 1302.2 0 86 212 161 1302.2 0 161 213 172 1302.2 0 172 214 149 1302.2 0 149 215 145 1302.2 0 145 216 343 1302.2 0 343 217 240 1302.2 0 240 218 257 1302.2 0 257 219 112 1302.2 0 112 220 44 1302.2 0 44 221 61 1302.2 0 61 222 89 1302.2 0 89 223 57 1302.2 0 57 224 180 1302.2 0 180 225 247 1302.2 0 247 226 394 1302.2 0 394 227 315 1302.2 0 315 228 307 1302.2 0 307 229 157 1302.2 0 157 230 74 1302.2 0 74 231 51 1302.2 0 51 232 80 1302.2 0 80 233 94 1302.2 0 94 234 56 1302.2 0 56 235 135 1302.2 0 135 236 127 1302.2 0 127 237 93 1302.2 0 93 238 47 1302.2 0 47 239 27 1302.2 0 27 240 33 1302.2 0 33 241 30 1302.2 0 30 242 48 1302.2 0 48 243 48 1302.2 0 48 244 115 1302.2 0 115 245 203 1302.2 0 203 246 146 1302.2 0 146 247 112 1302.2 0 112 248 62 1302.2 0 62 249 55 1302.2 0 55 250 54 1302.2 0 54 251 149 1302.2 0 149 252 154 1302.2 0 154 253 184 1302.2 0 184 254 363 1302.2 0 363 255 394 1302.2 0 394 256 288 1302.2 0 288 257 99 1302.2 0 99 258 59 1302.2 0 59 259 43 1302.2 0 43 260 20 1302.2 0 20 261 23 1302.2 0 23 262 24 1302.2 0 24 263 38 1302.2 0 38 264 78 1302.2 0 78 265 94 1302.2 0 94 266 224 1302.2 0 224 267 173 1302.2 0 173 268 120 1302.2 0 120 269 76 1302.2 0 76 270 93 1302.2 0 93 271 119 1302.2 0 119 272 93 1302.2 0 93 273 118 1302.2 0 118 274 202 1302.2 0 202 275 353 1302.2 0 353 276 116 1302.2 0 116 277 84 1302.2 0 84 278 104 1302.2 0 104 279 148 1302.2 0 148 280 248 1302.2 0 248 281 413 1302.2 0 413 282 477 1302.2 0 477 283 352 1302.2 0 352 284 80 1302.2 0 80 285 88 1302.2 0 88 286 41 1302.2 0 41 287 112 1302.2 0 112 288 45 1302.2 0 45 289 238 1302.2 0 238 290 445 1302.2 0 445 291 442 1302.2 0 442 292 322 1302.2 0 322 293 284 1302.2 0 284 294 156 1302.2 0 156 295 138 1302.2 0 138 296 142 1302.2 0 142 297 29 1302.2 0 29 298 30 1302.2 0 30 299 62 1302.2 0 62 300 31 1302.2 0 31 301 32 1302.2 0 32 Finished in 5.65 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 140,072 Reads with adapters: 864 (0.6%) Reads that were too short: 74 (0.1%) Reads written (passing filters): 790 (0.6%) Total basepairs processed: 25,504,761 bp Total written (filtered): 209,053 bp (0.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 864 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 13 0.0 3 0 8 0 5 17 687 0.0 3 654 30 2 1 18 16 0.0 3 0 1 14 1 19 11 0.0 3 0 0 0 11 20 4 0.0 3 3 0 1 23 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 51 11 0.0 3 0 0 0 11 54 5 0.0 3 0 0 0 5 55 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 23 0.0 3 0 0 0 23 86 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 136 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 148 4 0.0 3 0 0 0 4 149 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 174 12 0.0 3 0 0 1 11 175 23 0.0 3 0 0 0 23 178 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 236 1 0.0 3 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (51 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 790 Reads with adapters: 79 (10.0%) Reads written (passing filters): 790 (100.0%) Total basepairs processed: 209,053 bp Total written (filtered): 208,807 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 79 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.8% C: 40.5% G: 13.9% T: 17.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 72 12.3 0 72 4 6 3.1 0 6 6 1 0.2 0 1