This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 179.85 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 2,932,302 (55.0%) Reads that were too short: 994,616 (18.6%) Reads written (passing filters): 1,937,686 (36.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 322,970,836 bp (20.1%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 2932302 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 205 1302.2 0 205 7 661 1302.2 0 661 8 302 1302.2 0 302 9 14150 1302.2 0 14150 10 25 1302.2 0 25 11 3 1302.2 0 3 12 2 1302.2 0 2 13 8 1302.2 0 8 14 7 1302.2 0 7 15 9 1302.2 0 9 16 7 1302.2 0 7 17 13 1302.2 0 13 18 11 1302.2 0 11 19 4 1302.2 0 4 20 4 1302.2 0 4 21 6 1302.2 0 6 22 5 1302.2 0 5 23 7 1302.2 0 7 24 6 1302.2 0 6 25 8 1302.2 0 8 26 4 1302.2 0 4 27 36 1302.2 0 36 28 13 1302.2 0 13 29 6 1302.2 0 6 30 6 1302.2 0 6 31 17 1302.2 0 17 32 14 1302.2 0 14 33 22 1302.2 0 22 34 43 1302.2 0 43 35 97 1302.2 0 97 36 4608 1302.2 0 4608 37 81 1302.2 0 81 38 22 1302.2 0 22 39 4 1302.2 0 4 40 4 1302.2 0 4 41 33 1302.2 0 33 42 59 1302.2 0 59 43 79 1302.2 0 79 44 3 1302.2 0 3 45 7 1302.2 0 7 46 10 1302.2 0 10 47 2 1302.2 0 2 48 7 1302.2 0 7 49 4 1302.2 0 4 50 14 1302.2 0 14 51 16 1302.2 0 16 52 51 1302.2 0 51 53 6 1302.2 0 6 54 2 1302.2 0 2 55 8 1302.2 0 8 56 8 1302.2 0 8 57 5 1302.2 0 5 58 13 1302.2 0 13 59 4 1302.2 0 4 60 7 1302.2 0 7 61 7 1302.2 0 7 62 8 1302.2 0 8 63 10 1302.2 0 10 64 4 1302.2 0 4 65 6 1302.2 0 6 66 4 1302.2 0 4 67 19 1302.2 0 19 68 89 1302.2 0 89 69 93 1302.2 0 93 70 123 1302.2 0 123 71 174 1302.2 0 174 72 224 1302.2 0 224 73 277 1302.2 0 277 74 214 1302.2 0 214 75 474 1302.2 0 474 76 371 1302.2 0 371 77 2278 1302.2 0 2278 78 7363 1302.2 0 7363 79 10558 1302.2 0 10558 80 13833 1302.2 0 13833 81 18929 1302.2 0 18929 82 19427 1302.2 0 19427 83 18441 1302.2 0 18441 84 19268 1302.2 0 19268 85 25885 1302.2 0 25885 86 20131 1302.2 0 20131 87 9198 1302.2 0 9198 88 9017 1302.2 0 9017 89 19486 1302.2 0 19486 90 8446 1302.2 0 8446 91 40674 1302.2 0 40674 92 5116 1302.2 0 5116 93 19487 1302.2 0 19487 94 12435 1302.2 0 12435 95 38231 1302.2 0 38231 96 17663 1302.2 0 17663 97 8352 1302.2 0 8352 98 3219 1302.2 0 3219 99 6289 1302.2 0 6289 100 5290 1302.2 0 5290 101 13343 1302.2 0 13343 102 12453 1302.2 0 12453 103 16627 1302.2 0 16627 104 10667 1302.2 0 10667 105 37489 1302.2 0 37489 106 26711 1302.2 0 26711 107 24024 1302.2 0 24024 108 11956 1302.2 0 11956 109 11730 1302.2 0 11730 110 10933 1302.2 0 10933 111 25477 1302.2 0 25477 112 18789 1302.2 0 18789 113 27788 1302.2 0 27788 114 18554 1302.2 0 18554 115 24014 1302.2 0 24014 116 7990 1302.2 0 7990 117 12631 1302.2 0 12631 118 13152 1302.2 0 13152 119 16846 1302.2 0 16846 120 18677 1302.2 0 18677 121 20084 1302.2 0 20084 122 14778 1302.2 0 14778 123 18359 1302.2 0 18359 124 18953 1302.2 0 18953 125 22493 1302.2 0 22493 126 28599 1302.2 0 28599 127 21722 1302.2 0 21722 128 17128 1302.2 0 17128 129 9604 1302.2 0 9604 130 16487 1302.2 0 16487 131 9225 1302.2 0 9225 132 19289 1302.2 0 19289 133 23213 1302.2 0 23213 134 22110 1302.2 0 22110 135 18735 1302.2 0 18735 136 13270 1302.2 0 13270 137 11674 1302.2 0 11674 138 10844 1302.2 0 10844 139 12325 1302.2 0 12325 140 14893 1302.2 0 14893 141 18582 1302.2 0 18582 142 11086 1302.2 0 11086 143 12379 1302.2 0 12379 144 11780 1302.2 0 11780 145 18258 1302.2 0 18258 146 21881 1302.2 0 21881 147 14584 1302.2 0 14584 148 21288 1302.2 0 21288 149 17545 1302.2 0 17545 150 20937 1302.2 0 20937 151 12578 1302.2 0 12578 152 17575 1302.2 0 17575 153 20880 1302.2 0 20880 154 20228 1302.2 0 20228 155 14785 1302.2 0 14785 156 13894 1302.2 0 13894 157 10725 1302.2 0 10725 158 13701 1302.2 0 13701 159 12391 1302.2 0 12391 160 14923 1302.2 0 14923 161 17219 1302.2 0 17219 162 20544 1302.2 0 20544 163 21931 1302.2 0 21931 164 19186 1302.2 0 19186 165 15845 1302.2 0 15845 166 11638 1302.2 0 11638 167 9737 1302.2 0 9737 168 9463 1302.2 0 9463 169 12507 1302.2 0 12507 170 12666 1302.2 0 12666 171 9830 1302.2 0 9830 172 16570 1302.2 0 16570 173 12272 1302.2 0 12272 174 24916 1302.2 0 24916 175 11553 1302.2 0 11553 176 9643 1302.2 0 9643 177 6646 1302.2 0 6646 178 10828 1302.2 0 10828 179 11967 1302.2 0 11967 180 15099 1302.2 0 15099 181 13775 1302.2 0 13775 182 9618 1302.2 0 9618 183 11127 1302.2 0 11127 184 10174 1302.2 0 10174 185 12869 1302.2 0 12869 186 15316 1302.2 0 15316 187 13285 1302.2 0 13285 188 12153 1302.2 0 12153 189 10189 1302.2 0 10189 190 13818 1302.2 0 13818 191 16283 1302.2 0 16283 192 17204 1302.2 0 17204 193 14890 1302.2 0 14890 194 10336 1302.2 0 10336 195 5119 1302.2 0 5119 196 6226 1302.2 0 6226 197 7180 1302.2 0 7180 198 13056 1302.2 0 13056 199 21270 1302.2 0 21270 200 10441 1302.2 0 10441 201 7790 1302.2 0 7790 202 7457 1302.2 0 7457 203 8478 1302.2 0 8478 204 8698 1302.2 0 8698 205 10638 1302.2 0 10638 206 10706 1302.2 0 10706 207 9119 1302.2 0 9119 208 8392 1302.2 0 8392 209 8049 1302.2 0 8049 210 7913 1302.2 0 7913 211 9632 1302.2 0 9632 212 9646 1302.2 0 9646 213 13656 1302.2 0 13656 214 10200 1302.2 0 10200 215 11793 1302.2 0 11793 216 9881 1302.2 0 9881 217 9533 1302.2 0 9533 218 12616 1302.2 0 12616 219 14039 1302.2 0 14039 220 10723 1302.2 0 10723 221 7211 1302.2 0 7211 222 8537 1302.2 0 8537 223 7819 1302.2 0 7819 224 11722 1302.2 0 11722 225 12701 1302.2 0 12701 226 11553 1302.2 0 11553 227 13173 1302.2 0 13173 228 10574 1302.2 0 10574 229 12368 1302.2 0 12368 230 13267 1302.2 0 13267 231 11682 1302.2 0 11682 232 6868 1302.2 0 6868 233 7072 1302.2 0 7072 234 9850 1302.2 0 9850 235 10383 1302.2 0 10383 236 12255 1302.2 0 12255 237 12410 1302.2 0 12410 238 12182 1302.2 0 12182 239 13627 1302.2 0 13627 240 10282 1302.2 0 10282 241 7942 1302.2 0 7942 242 7589 1302.2 0 7589 243 7320 1302.2 0 7320 244 7411 1302.2 0 7411 245 10195 1302.2 0 10195 246 12205 1302.2 0 12205 247 19036 1302.2 0 19036 248 15071 1302.2 0 15071 249 12073 1302.2 0 12073 250 9719 1302.2 0 9719 251 10819 1302.2 0 10819 252 8710 1302.2 0 8710 253 10369 1302.2 0 10369 254 8915 1302.2 0 8915 255 11533 1302.2 0 11533 256 12747 1302.2 0 12747 257 15731 1302.2 0 15731 258 11074 1302.2 0 11074 259 10862 1302.2 0 10862 260 6522 1302.2 0 6522 261 6229 1302.2 0 6229 262 6096 1302.2 0 6096 263 5840 1302.2 0 5840 264 7664 1302.2 0 7664 265 8247 1302.2 0 8247 266 10516 1302.2 0 10516 267 14604 1302.2 0 14604 268 11514 1302.2 0 11514 269 11732 1302.2 0 11732 270 8324 1302.2 0 8324 271 7814 1302.2 0 7814 272 7610 1302.2 0 7610 273 10142 1302.2 0 10142 274 10750 1302.2 0 10750 275 9669 1302.2 0 9669 276 7859 1302.2 0 7859 277 6989 1302.2 0 6989 278 8067 1302.2 0 8067 279 11981 1302.2 0 11981 280 12354 1302.2 0 12354 281 6872 1302.2 0 6872 282 9407 1302.2 0 9407 283 6091 1302.2 0 6091 284 10655 1302.2 0 10655 285 9852 1302.2 0 9852 286 11628 1302.2 0 11628 287 12337 1302.2 0 12337 288 9175 1302.2 0 9175 289 10081 1302.2 0 10081 290 6857 1302.2 0 6857 291 9887 1302.2 0 9887 292 8466 1302.2 0 8466 293 12127 1302.2 0 12127 294 10536 1302.2 0 10536 295 10740 1302.2 0 10740 296 7055 1302.2 0 7055 297 5834 1302.2 0 5834 298 3599 1302.2 0 3599 299 3870 1302.2 0 3870 300 5820 1302.2 0 5820 301 17178 1302.2 0 17178 Finished in 80.99 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 1,937,686 Reads with adapters: 1,107 (0.1%) Reads that were too short: 347 (0.0%) Reads written (passing filters): 760 (0.0%) Total basepairs processed: 322,970,836 bp Total written (filtered): 187,231 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1107 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 0 5 18 2 0.0 3 0 0 0 0 2 19 14 0.0 3 0 11 0 0 3 20 478 0.0 4 463 8 0 1 6 21 9 0.0 4 1 4 0 1 3 22 115 0.0 4 103 1 0 0 11 23 2 0.0 4 0 0 0 0 2 24 4 0.0 4 0 0 0 0 4 25 3 0.0 4 0 0 0 1 2 26 1 0.0 4 0 0 0 0 1 27 2 0.0 4 0 0 0 0 2 28 1 0.0 4 0 0 0 0 1 29 2 0.0 4 0 0 0 0 2 30 2 0.0 4 0 0 0 0 2 31 3 0.0 4 0 0 0 0 3 32 5 0.0 4 0 0 0 0 5 33 7 0.0 4 0 0 0 1 6 35 3 0.0 4 0 0 0 0 3 36 6 0.0 4 0 0 0 0 6 38 6 0.0 4 0 0 0 0 6 39 2 0.0 4 0 0 0 1 1 41 4 0.0 4 0 0 0 1 3 42 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 45 1 0.0 4 0 0 0 0 1 46 3 0.0 4 0 0 0 0 3 47 4 0.0 4 0 0 0 0 4 48 4 0.0 4 0 0 0 0 4 49 3 0.0 4 0 0 0 1 2 50 7 0.0 4 0 0 0 0 7 51 5 0.0 4 0 0 0 0 5 52 3 0.0 4 0 0 0 0 3 53 2 0.0 4 0 0 0 0 2 54 4 0.0 4 0 0 0 1 3 55 5 0.0 4 0 0 0 1 4 56 1 0.0 4 0 0 0 0 1 57 6 0.0 4 0 0 0 1 5 58 5 0.0 4 0 0 0 0 5 59 5 0.0 4 0 0 0 0 5 60 4 0.0 4 0 0 0 0 4 61 3 0.0 4 0 0 0 0 3 62 1 0.0 4 0 0 0 0 1 63 6 0.0 4 0 0 0 0 6 64 3 0.0 4 0 0 0 0 3 65 1 0.0 4 0 0 0 0 1 66 6 0.0 4 0 0 0 0 6 67 3 0.0 4 0 0 0 0 3 68 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 70 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 4 0.0 4 0 0 0 0 4 74 2 0.0 4 0 0 0 0 2 75 3 0.0 4 0 0 0 0 3 76 6 0.0 4 0 0 0 0 6 77 2 0.0 4 0 0 0 0 2 78 3 0.0 4 0 0 0 1 2 79 7 0.0 4 0 0 0 1 6 80 3 0.0 4 0 0 0 0 3 81 3 0.0 4 0 0 0 0 3 82 7 0.0 4 0 0 0 0 7 83 5 0.0 4 0 0 0 0 5 84 6 0.0 4 0 0 0 0 6 85 3 0.0 4 0 0 0 0 3 87 3 0.0 4 0 0 0 0 3 88 5 0.0 4 0 0 0 0 5 89 2 0.0 4 0 0 0 0 2 90 2 0.0 4 0 0 0 0 2 91 5 0.0 4 0 0 0 0 5 92 4 0.0 4 0 0 0 0 4 93 6 0.0 4 0 0 0 0 6 94 4 0.0 4 0 0 0 0 4 95 2 0.0 4 0 0 0 0 2 96 4 0.0 4 0 0 0 0 4 97 6 0.0 4 0 0 0 0 6 98 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 100 5 0.0 4 0 0 0 0 5 101 4 0.0 4 0 0 0 0 4 102 3 0.0 4 0 0 0 1 2 103 3 0.0 4 0 0 0 0 3 104 6 0.0 4 0 0 0 0 6 105 1 0.0 4 0 0 0 0 1 106 5 0.0 4 0 0 0 1 4 107 6 0.0 4 0 0 0 0 6 108 1 0.0 4 0 0 0 0 1 109 4 0.0 4 0 0 0 0 4 110 5 0.0 4 0 0 0 0 5 111 6 0.0 4 0 0 0 0 6 112 5 0.0 4 0 0 0 0 5 113 3 0.0 4 0 0 0 0 3 114 1 0.0 4 0 0 0 0 1 115 3 0.0 4 0 0 0 0 3 116 2 0.0 4 0 0 0 0 2 117 5 0.0 4 0 0 0 0 5 118 3 0.0 4 0 0 0 1 2 119 6 0.0 4 0 0 0 0 6 120 3 0.0 4 0 0 0 0 3 121 5 0.0 4 0 0 0 0 5 122 5 0.0 4 0 0 0 0 5 123 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 9 0.0 4 0 0 0 0 9 128 2 0.0 4 0 0 0 0 2 129 2 0.0 4 0 0 0 0 2 130 4 0.0 4 0 0 0 0 4 131 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 133 4 0.0 4 0 0 0 0 4 134 1 0.0 4 0 0 0 0 1 135 4 0.0 4 0 0 0 1 3 137 2 0.0 4 0 0 0 0 2 138 2 0.0 4 0 0 0 0 2 139 2 0.0 4 0 0 0 0 2 140 2 0.0 4 0 0 0 0 2 141 2 0.0 4 0 0 0 0 2 142 3 0.0 4 0 0 0 0 3 143 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 2 0.0 4 0 0 0 0 2 146 3 0.0 4 0 0 0 1 2 147 5 0.0 4 0 0 0 0 5 148 4 0.0 4 0 0 0 0 4 149 2 0.0 4 0 0 0 0 2 150 2 0.0 4 0 0 0 0 2 152 2 0.0 4 0 0 0 0 2 153 2 0.0 4 0 0 0 0 2 154 5 0.0 4 0 0 0 0 5 156 2 0.0 4 0 0 0 0 2 157 2 0.0 4 0 0 0 0 2 158 3 0.0 4 0 0 0 0 3 159 2 0.0 4 0 0 0 0 2 160 2 0.0 4 0 0 0 0 2 161 2 0.0 4 0 0 0 0 2 163 2 0.0 4 0 0 0 1 1 164 3 0.0 4 0 0 0 0 3 166 1 0.0 4 0 0 0 0 1 167 3 0.0 4 0 0 0 0 3 168 4 0.0 4 0 0 0 0 4 169 2 0.0 4 0 0 0 0 2 170 3 0.0 4 0 0 0 0 3 172 2 0.0 4 0 0 0 0 2 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 176 2 0.0 4 0 0 0 0 2 177 2 0.0 4 0 0 0 0 2 179 1 0.0 4 0 0 0 0 1 180 3 0.0 4 0 0 0 0 3 181 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 195 2 0.0 4 0 0 0 0 2 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 2 0.0 4 0 0 0 0 2 205 2 0.0 4 0 0 0 0 2 206 2 0.0 4 0 0 0 0 2 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 2 0.0 4 0 0 0 0 2 228 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 760 Reads with adapters: 90 (11.8%) Reads written (passing filters): 760 (100.0%) Total basepairs processed: 187,231 bp Total written (filtered): 165,530 bp (88.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 90 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 95.6% C: 0.0% G: 4.4% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 11.9 0 2 11 5 0.0 1 0 5 234 1 0.0 1 1 259 2 0.0 1 2 261 73 0.0 1 70 3 262 6 0.0 1 5 1 263 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.